scholarly journals Examining the Transmission of Visible Light through Electrospun Nanofibrous PCL Scaffolds for Corneal Tissue Engineering

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3191
Author(s):  
Marcus Himmler ◽  
Dirk W. Schubert ◽  
Thomas A. Fuchsluger
Keyword(s):  
Tissue Engineering ◽  
Visible Light ◽  
Light Transmission ◽  
Fiber Diameter ◽  
Surrounding Medium ◽  
Wound Dressings ◽  
Human Cornea ◽  
Corneal Tissue ◽  
Nanofibrous Scaffolds ◽  
Vis Spectroscopy

The transparency of nanofibrous scaffolds is of highest interest for potential applications like corneal wound dressings in corneal tissue engineering. In this study, we provide a detailed analysis of light transmission through electrospun polycaprolactone (PCL) scaffolds. PCL scaffolds were produced via electrospinning, with fiber diameters in the range from (35 ± 13) nm to (167 ± 35) nm. Light transmission measurements were conducted using UV–vis spectroscopy in the range of visible light and analyzed with respect to the influence of scaffold thickness, fiber diameter, and surrounding medium. Contour plots were compiled for a straightforward access to light transmission values for arbitrary scaffold thicknesses. Depending on the fiber diameter, transmission values between 15% and 75% were observed for scaffold thicknesses of 10 µm. With a decreasing fiber diameter, light transmission could be improved, as well as with matching refractive indices of fiber material and medium. For corneal tissue engineering, scaffolds should be designed as thin as possible and fabricated from polymers with a matching refractive index to that of the human cornea. Concerning fiber diameter, smaller fiber diameters should be favored for maximizing graft transparency. Finally, a novel, semi-empirical formulation of light transmission through nanofibrous scaffolds is presented.

Nanofibrous scaffolds for corneal tissue engineering

2013 ◽  
Vol 91 ◽  
pp. 0-0
Author(s):  
T FUCHSLUGER ◽  
T BAHNERS ◽  
M CZUGALA ◽  
J GUTMANN ◽  
S SALEHI
Keyword(s):  
Tissue Engineering ◽  
Corneal Tissue ◽  
Nanofibrous Scaffolds

scholarly journals Prospects and Challenges of Translational Corneal Bioprinting

2020 ◽  
Vol 7 (3) ◽  
pp. 71 ◽  
Author(s):  
Matthias Fuest ◽  
Gary Hin-Fai Yam ◽  
Jodhbir S. Mehta ◽  
Daniela F. Duarte Campos
Keyword(s):  
Tissue Engineering ◽  
Ex Vivo ◽  
Corneal Transplantation ◽  
Human Cornea ◽  
Corneal Tissue ◽  
Full Thickness ◽  
Future Directions ◽  
Spatial Control

Corneal transplantation remains the ultimate treatment option for advanced stromal and endothelial disorders. Corneal tissue engineering has gained increasing interest in recent years, as it can bypass many complications of conventional corneal transplantation. The human cornea is an ideal organ for tissue engineering, as it is avascular and immune-privileged. Mimicking the complex mechanical properties, the surface curvature, and stromal cytoarchitecure of the in vivo corneal tissue remains a great challenge for tissue engineering approaches. For this reason, automated biofabrication strategies, such as bioprinting, may offer additional spatial control during the manufacturing process to generate full-thickness cell-laden 3D corneal constructs. In this review, we discuss recent advances in bioprinting and biomaterials used for in vitro and ex vivo corneal tissue engineering, corneal cell-biomaterial interactions after bioprinting, and future directions of corneal bioprinting aiming at engineering a full-thickness human cornea in the lab.

Electrospun hybrid nanofibrous meshes with adjustable performance for potential use in soft tissue engineering

2021 ◽  
pp. 004051752110639
Author(s):  
Ye Qi ◽  
Huiyuan Zhai ◽  
Yaning Sun ◽  
Hongxing Xu ◽  
Shaohua Wu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Soft Tissue ◽  
Fiber Diameter ◽  
Fiber Morphology ◽  
Surface Hydrophilicity ◽  
Nanofibrous Scaffolds ◽  
Soft Tissue Engineering ◽  
The Mean

Electrospun nanofibrous scaffolds have gained extensive attention in the fields of soft tissue engineering and regenerative medicine. In this study, a series of biodegradable nanofibrous meshes were fabricated by electrospinning poly(ε-caprolactone) (PCL) and poly( p-dioxanone) (PPDO) blends with various mass ratios. All the as-developed PCL/PPDO nanofibrous meshes possessed smooth and highly aligned fiber morphology. The mean fiber diameter was 521.5 ± 76.6 nm for PCL meshes and 485.8 ± 88.9 nm for PPDO meshes, and the mean fiber diameter seemed to present a decreasing tendency with the increasing of the PPDO component. For pure PCL meshes, the contact angle was about 117.5 ± 1.6°, the weight loss ratio was roughly 0.2% after 10 weeks of degradation, and the tensile strength was 41.2 ± 2.3 MPa in the longitudinal direction and 4.2 ± 0.1 MPa in the transverse direction. It was found that the surface hydrophilicity and in vitro degradation properties of PCL/PPDO meshes apparently increased, but the mechanical properties of PCL/PPDO meshes obviously decreased when more PPDO component was introduced. The biological tests showed that 4:1 PCL/PPDO nanofibrous meshes and 1:1 PCL/PPDO nanofibrous meshes could obviously promote the adhesion and proliferation of human adipose derived mesenchymal stem cells more than pure PCL and PPDO meshes and 1:4 PCL/PPDO meshes. The results demonstrated that it is feasible to adjust the surface hydrophilicity, degradation profile, and mechanical properties as well as biological properties of as-obtained nanofibrous meshes by blending PCL and PPDO components. This study provides meaningful reference and guidance for the design and development of PCL/PPDO hybrid nanofibrous scaffolds for soft tissue engineering research and application.

Electrospun nanofibrous scaffolds and corneal tissue engineering

2014 ◽  
Vol 92 ◽  
pp. 0-0
Author(s):  
S SALEHI ◽  
T BAHNERS ◽  
J GUTMANN ◽  
T FUCHSLUGER
Keyword(s):  
Tissue Engineering ◽  
Corneal Tissue ◽  
Nanofibrous Scaffolds

scholarly journals Graphene oxide-modified electrospun polyvinyl alcohol nanofibrous scaffolds with potential as skin wound dressings

RSC Advances ◽  
2017 ◽  
Vol 7 (46) ◽  
pp. 28826-28836 ◽  
Author(s):  
Qiang Zhang ◽  
Qiaoyue Du ◽  
Yanan Zhao ◽  
Feixiang Chen ◽  
Zijian Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Polyvinyl Alcohol ◽  
Potential Application ◽  
Skin Wound ◽  
Wound Dressings ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds ◽  
Good Biocompatibility

Graphene oxide-modified electrospun polyvinyl alcohol nanofibrous scaffolds exhibit good biocompatibility and have potential application in skin tissue engineering.

scholarly journals Visible Light-Cured Antibacterial Collagen Hydrogel Containing Water-Solubilized Triclosan for Improved Wound Healing

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2270
Author(s):  
Longhao Jin ◽  
Kyeongsoon Park ◽  
Yihyun Yoon ◽  
Hyeon Soo Kim ◽  
Hyeon Ji Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Visible Light ◽  
Wound Dressing ◽  
Wound Dressings ◽  
Animal Testing ◽  
Normal Wound Healing ◽  
Collagen Hydrogel ◽  
Vis Spectroscopy ◽  
Clinical Potential

Infection is one of several factors that can delay normal wound healing. Antibacterial wound dressings can therefore promote normal wound healing. In this study, we prepared an antibacterial wound dressing, consisting of visible light-cured methacrylated collagen (ColMA) hydrogel and a 2-hydroxypropyl-beta-cyclodextrin (HP-β-CD)/triclosan (TCS) complex (CD-ic-TCS), and evaluated its wound healing effects in vivo. The 1H NMR spectra of ColMA and CD-ic-TCS revealed characteristic peaks at 1.73, 5.55, 5.94, 6.43, 6.64, 6.84, 6.95, 7.31, and 7.55 ppm, indicating successful preparation of the two material types. In addition, ultraviolet–visible (UV–vis) spectroscopy proved an inclusion complex formation between HP-β-CD and TCS, judging by a unique peak observed at 280 cm−1. Furthermore, ColMA/CD-ic-TCS exhibited an interconnected porous structure, controlled release of TCS, good biocompatibility, and antibacterial activity. By in vivo animal testing, we found that ColMA/CD-ic-TCS had a superior wound healing capacity, compared to the other hydrocolloids evaluated, due to synergistic interaction between ColMA and CD-ic-TCS. Together, our findings indicate that ColMA/CD-ic-TCS has a clinical potential as an antibacterial wound dressing.

scholarly journals Effects of Chitosan Alkali Pretreatment on the Preparation of Electrospun PCL/Chitosan Blend Nanofibrous Scaffolds for Tissue Engineering Application

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Fatemeh Roozbahani ◽  
Naznin Sultana ◽  
Ahmad Fauzi Ismail ◽  
Hamed Nouparvar
Keyword(s):  
Tissue Engineering ◽  
Molecular Weight ◽  
Treatment Time ◽  
Engineering Application ◽  
Electrospinning Process ◽  
Wound Dressings ◽  
Tissue Engineering Application ◽  
Sem Images ◽  
Nanofibrous Scaffolds ◽  
3D Network

Recently, nanofibrous scaffolds have been used in the field of biomedical engineering as wound dressings, tissue engineering scaffolds, and drug delivery applications. The electrospun nanofibrous scaffolds can be used as carriers for several types of drugs, genes, and growth factors. PCL is one of the most commonly applied synthetic polymers for medical use because of its biocompatibility and slow biodegradability. PCL is hydrophobic and has no cell recognition sites on its structure. Electrospinning of chitosan and PCL blend was investigated in formic acid/acetic acid as the solvent with different PCL/chitosan ratios. High viscosity of chitosan solutions makes difficulties in the electrospinning process. Strong hydrogen bonds in a 3D network in acidic condition prevent the movement of polymeric chains exposed to the electrical field. Consequently, the amount of chitosan in PCL/chitosan blend was limited and more challenging when the concentration of PCL increases. The treatment of chitosan in alkali condition under high temperature reduced its molecular weight. Longer treatment time further decreased the molecular weight of chitosan and hence its viscosity. Electrospinning of PCL/chitosan blend was possible at higher chitosan ratio, and SEM images showed a decrease in fiber diameter and narrower distribution with increase in the chitosan ratio.

scholarly journals Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

10.3791/56308 ◽  
2018 ◽  
Author(s):  
Rabab Sharif ◽  
Shrestha Priyadarsini ◽  
Tyler G. Rowsey ◽  
Jian-Xing Ma ◽  
Dimitrios Karamichos
Keyword(s):  
Tissue Engineering ◽  
Human Cornea ◽  
Corneal Tissue

scholarly journals Fabrication of Micro/Nanofibrous Scaffolds using a Robotic Manipulator and Their Application for Tissue Engineering

2020 ◽  
Author(s):  
Andrii Shynkarenko ◽  
Dekel Azulay ◽  
Sarka Hauzerova ◽  
Andrea Klapstova ◽  
Michal Moucka ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Dominant Role ◽  
Robotic Manipulator ◽  
Wound Dressings ◽  
Scanning Calorimetry ◽  
Tissue Engineering Scaffolds ◽  
Nanofibrous Scaffolds ◽  
Dimensional Network ◽  
Wide Range

Abstract Background: Nanofibrous materials currently find a wide range of medical and bioengineering applications including tissue-engineering scaffolds, sutures, and wound dressings. Recently, production of nanofibrous materials via Electrospinning has played a dominant role in this area. Here we introduce an alternative method, which we call the drawing method, which allows us to produce individual micro and nanofibers and to position them precisely into a two-dimensional network. Results: The creation of such nano or micro fibrous networks is enabled thanks to a special arm-like robotic manipulator that we have designed, including its control system software. In this work we produced and tested microfibrous scaffolds of precise geometry made of two different biodegradable polymers: Polycaprolactone and Polylactide – Polycaprolactone copolymer. The microfibrous networks produced thereby were analyzed using a scanning electronic microscope and tested in vitro for cell adhesion and proliferation. The crystallinity of the resulting manufactured polymeric structures was evaluated using differential scanning calorimetry. Conclusions: The mechanical drawing of individual microfibers presented in this article is a promising method to produce precisely oriented nano and microfibrous structures for technical as well as bioengineering applications. Our results indicate that the mechanical drawing of microfibers expands the possibilities for the preparation of tissue engineering scaffolds. Therefore, we believe that the range of applications of mechanical fiber drawing may soon expand.

ELECTROSPUN GELATIN NANOFIBROUS SCAFFOLDS – APPLICATIONS IN TISSUE ENGINEERING

2021 ◽  
Author(s):  
Katarina Virijević ◽  
◽  
Jelena Grujić ◽  
Milena Jovanović ◽  
Nikolina Kastratović ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fiber Diameter ◽  
Biomedical Application ◽  
Liquid Solution ◽  
Physical Structure ◽  
Electrospinning Process ◽  
Nanofibrous Scaffolds ◽  
Electrospun Gelatin ◽  
Native Skin

Electrospinning is highly used technique in the tissue engineering field, particularly in biomedical application [1]. The constricted concepts of this process are based on generate nonwoven nanofibers. The method involves high voltage electricity which is applied to the liquid solution and a collector, which lets the solution force out from a nozzle forming a jet. The jet formed fibers under influence of electrostatic forces concentrated and deposited these on the collector. Main objective of this study was to fabricate gelatin scaffolds with micro/nano-scale for successful wound dressing. Gelatin can mimic the chemical composition, physical structure and structure of the native skin extracellular matrix (ECM). However, the first and main principle in this study is the optimization of parameters of the electrospinning process. The used parameters have a crucial role in obtaining suitable fibers for further cell seeding and cell growth in vitro. With the use of series of various biocompatible polymers and solvents, solutions were tested in various electrospinning settings in order to produce microscale fibers. The scaffolds were analysed with scanning electron microscope images for fiber diameter measurement.

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