scholarly journals Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

10.3791/57319 ◽  
2018 ◽  
Author(s):  
Suzan Wetzels ◽  
Mitchell Bijnen ◽  
Erwin Wijnands ◽  
Erik A.L. Biessen ◽  
Casper G. Schalkwijk ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Adipose Tissue ◽  
Immune Cells ◽  
Human Adipose Tissue

scholarly journals Characterization of immune cells in psoriatic adipose tissue

2014 ◽  
Vol 12 (1) ◽  
Author(s):  
Shawn Rose ◽  
Elena Stansky ◽  
Pradeep K Dagur ◽  
Leigh Samsel ◽  
Elizabeth Weiner ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Immune Cells

scholarly journals STAT4 contributes to adipose tissue inflammation and atherosclerosis

2015 ◽  
Vol 227 (1) ◽  
pp. 13-24 ◽  
Author(s):  
A D Dobrian ◽  
M A Hatcher ◽  
J J Brotman ◽  
E V Galkina ◽  
P Taghavie-Moghadam ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Cardiovascular Disease ◽  
Flow Cytometry ◽  
Adipose Tissue ◽  
Immune Cells ◽  
Indirect Effects ◽  
Western Diet ◽  
Plaque Burden ◽  
Adipose Tissue Inflammation ◽  
Similar Body

Adipose tissue (AT) inflammation is an emerging factor contributing to cardiovascular disease. STAT4 is a transcription factor expressed in adipocytes and in immune cells and contributes to AT inflammation and insulin resistance in obesity. The objective of this study was to determine the effect of STAT4 deficiency on visceral and peri-aortic AT inflammation in a model of atherosclerosis without obesity. Stat4−/−Apoe−/− mice and Apoe−/− controls were kept either on chow or Western diet for 12 weeks. Visceral and peri-aortic AT were collected and analyzed for immune composition by flow cytometry and for cytokine/chemokine expression by real-time PCR. Stat4−/−Apoe−/− and Apoe−/− mice had similar body weight, plasma glucose, and lipids. Western diet significantly increased macrophage, CD4+, CD8+, and NK cells in peri-aortic and visceral fat in Apoe−/− mice. In contrast, in Stat4−/−Apoe−/− mice, a Western diet failed to increase the percentage of immune cells infiltrating the AT. Also, IL12p40, TNFa, CCL5, CXCL10, and CX3CL1 were significantly reduced in the peri-aortic fat in Stat4−/−Apoe−/− mice. Importantly, Stat4−/−Apoe−/− mice on a Western diet had significantly reduced plaque burden vs Apoe−/− controls. In conclusion, STAT4 deletion reduces inflammation in peri-vascular and visceral AT and this may contribute via direct or indirect effects to reduced atheroma formation.

scholarly journals Viscoelastic characterization of diabetic and non-diabetic human adipose tissue

Biorheology ◽  
2020 ◽  
Vol 57 (1) ◽  
pp. 15-26
Author(s):  
Benjamin A. Juliar ◽  
Clarissa Strieder-Barboza ◽  
Monita Karmakar ◽  
Carmen G. Flesher ◽  
Nicki A. Baker ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Human Adipose Tissue

scholarly journals A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation

Processes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 88
Author(s):  
Ilaria Roato ◽  
Federico Mussano ◽  
Simone Reano ◽  
Filippo Boriani ◽  
Andrea Margara ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Stromal Vascular Fraction ◽  
Fold Increase ◽  
Human Adipose Tissue ◽  
Promising Tool ◽  
Filter Size ◽  
Tissue Recovery

This work aims to characterize a new method to recover low-manipulated human adipose tissue, enriched with adipose tissue-derived mesenchymal stem cells (ATD-MSCs) for autologous use in regenerative medicine applications. Lipoaspirated fat collected from patients was processed through Lipocell, a Class II-a medical device for dialysis of adipose tissue, by varying filter sizes and washing solutions. ATD-MSC yield was measured with flow cytometry after stromal vascular fraction (SVF) isolation in fresh and cultured samples. Purification from oil and blood was measured after centrifugation with spectrophotometer analysis. Extracellular matrix preservation was assessed through hematoxylin and eosin (H&E) staining and biochemical assay for total collagen, type-2 collagen, and glycosaminoglycans (GAGs) quantification. Flow cytometry showed a two-fold increase of ATD-MSC yield in treated samples in comparison with untreated lipoaspirate; no differences where reported when varying filter size. The association of dialysis and washing thoroughly removed blood and oil from samples. Tissue architecture and extracellular matrix integrity were unaltered after Lipocell processing. Dialysis procedure associated with Ringer’s lactate preserves the proliferation ability of ATD-MSCs in cell culture. The characterization of the product showed that Lipocell is an efficient method for purifying the tissue from undesired byproducts and preserving ATD-MSC vitality and extracellular matrix (ECM) integrity, resulting in a promising tool for regenerative medicine applications.

Effect of Temperature and Freezing On Human Adipose Tissue Material Properties Characterized by High-Rate Indentation-Puncture Testing

2021 ◽  
Author(s):  
Zhaonan Sun ◽  
Bronislaw Gepner ◽  
Sang-hyun Lee ◽  
Michelle Oyen ◽  
Josh Rigby ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Adipose Tissue ◽  
Material Properties ◽  
Injury Risk ◽  
Seat Belt ◽  
Human Adipose Tissue ◽  
High Rate ◽  
Effect Of Temperature ◽  
Frozen Tissue

Abstract The characterization of human subcutaneous adipose tissue (SAT) under high-rate loading is valuable for development of biofidelic finite element human body models (FE-HBMs) to predict seat belt-pelvis interaction and injury risk in vehicle crash simulations. While material characterization of SAT has been performed at 25°C or 37°C, the effect of temperature on mechanical properties of SAT under high-rate and large-deformation loading has not been investigated. Similarly, while freezing is the most common preservation technique for cadaveric specimens, the effect of freeze-thaw on the mechanical properties of SAT is also absent from the literature. Therefore, the aim of this study was to determine the effect of freezing and temperature on mechanical properties of human SAT. Fresh and previously frozen human SAT specimens were obtained and tested at 25°C and 37°C. High-rate indentation and puncture tests were performed, and indentation-puncture force-depth responses were obtained. While the chance of material failure was found to be different between temperatures and between fresh and previously frozen tissue, statistical analyses revealed that temperature and freezing did not change the shear modulus and failure characteristics of SAT. Therefore, the results of the current study indicated that SAT material properties characterized from either fresh or frozen tissue at either 25°C or 37°C could be used for enhancing the biofidelity of FE-HBMs.

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