scholarly journals Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

10.3791/58166 ◽  
2018 ◽  
Author(s):  
Roberto Pech-Kú ◽  
J. Armando Muñoz-Sánchez ◽  
Miriam Monforte-González ◽  
Felipe Vázquez-Flota ◽  
Beatriz A. Rodas-Junco ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzymatic Activity ◽  
Plant Cell ◽  
Cell Suspensions ◽  
Caffeine Synthase ◽  
Caffeine Extraction

An Airlift Column Bioreactor Suitable for Large-Scale Cultivation of Plant Cell Suspensions

1984 ◽  
Vol 35 (4) ◽  
pp. 531-537 ◽  
Author(s):  
N. J. SMART ◽  
M. W. FOWLER
Keyword(s):  
Plant Cell ◽  
Large Scale ◽  
Cell Suspensions ◽  
Column Bioreactor ◽  
Large Scale Cultivation

Thymidine kinase activation of ganciclovir in recurrent malignant gliomas: a gene-marking and neuropathological study

2000 ◽  
Vol 92 (5) ◽  
pp. 804-811 ◽  
Author(s):  
Griffith R. Harsh ◽  
Thomas S. Deisboeck ◽  
David N. Louis ◽  
John Hilton ◽  
Michael Colvin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzymatic Activity ◽  
Thymidine Kinase ◽  
Tumor Cells ◽  
Malignant Gliomas ◽  
Retroviral Vectors ◽  
Content Type ◽  
Tumor Bed ◽  
Immunohistochemical Evidence ◽  
Fine Print

Object. The gene therapy paradigm of intratumoral activation of ganciclovir (GCV) following transduction of tumor cells by retroviral vectors bearing the thymidine kinase (tk) gene has produced dramatic remissions of malignant gliomas in animal models. In human trials, although the technique has been deemed safe, little antitumor effect has been demonstrated. To evaluate the basis of this inefficacy in human gliomas, the authors conducted a gene-marking trial involving neuropathological and biochemical studies of treated tumor specimens.Methods. Five patients with malignant recurrent gliomas underwent stereotactic biopsy sampling and intratumoral implantation procedures with three aliquots of 106 vector-producing cells (VPCs) in columns. After 5 days, the tumor was resected and the tumor bed reimplanted with VPCs, and a course of GCV was given. Patients received clinical and radiological follow up for 6 months. Tumor specimens were analyzed neuropathologically and for tk gene expression by anti-TK immunohistochemistry and TK enzymatic activity.Four patients tolerated the treatment well but experienced tumor progression. The other developed an abscess after the second operation and died. Increased TK enzymatic activity was demonstrated in the one tumor specimen analyzed. Immunohistochemical evidence of tk gene expression was limited to VPCs. Transduction of tumor cells was not seen. Viable tumor cells were seen near VPCs containing TK. The lymphocytic immune response was mild.Conclusions. Except for the risk of infection inherent in reoperation, this tk—GCV paradigm was both feasible and safe. Pathological studies indicated that limited dissemination of VPCs and vector from the infusion site and failure to transduce tumor cells with the tk gene are major barriers to efficacy.

The Digestive Function in Developing Fish Larvae and Fry. From Molecular Gene Expression to Enzymatic Activity

2018 ◽  
pp. 51-86 ◽  
Author(s):  
Manuel Yúfera ◽  
Francisco J. Moyano ◽  
Gonzalo Martínez-Rodríguez
Keyword(s):  
Gene Expression ◽  
Enzymatic Activity ◽  
Fish Larvae ◽  
Digestive Function

scholarly journals Caspase-8 promotes c-Rel–dependent inflammatory cytokine expression and resistance againstToxoplasma gondii

2019 ◽  
pp. 201820529 ◽  
Author(s):  
Alexandra A. DeLaney ◽  
Corbett T. Berry ◽  
David A. Christian ◽  
Andrew Hart ◽  
Elisabet Bjanes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzymatic Activity ◽  
Inflammatory Cytokine ◽  
Cytokine Expression ◽  
Cytokine Gene Expression ◽  
Caspase 8 ◽  
Cytokine Gene ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene ◽  
Monocyte Recruitment

Caspase-8 is a key integrator of cell survival and cell death decisions during infection and inflammation. Following engagement of tumor necrosis factor superfamily receptors or certain Toll-like receptors (TLRs), caspase-8 initiates cell-extrinsic apoptosis while inhibiting RIPK3-dependent programmed necrosis. In addition, caspase-8 has an important, albeit less well understood, role in cell-intrinsic inflammatory gene expression. Macrophages lacking caspase-8 or the adaptor FADD have defective inflammatory cytokine expression and inflammasome priming in response to bacterial infection or TLR stimulation. How caspase-8 regulates cytokine gene expression, and whether caspase-8–mediated gene regulation has a physiological role during infection, remain poorly defined. Here we demonstrate that both caspase-8 enzymatic activity and scaffolding functions contribute to inflammatory cytokine gene expression. Caspase-8 enzymatic activity was necessary for maximal expression ofIl1bandIl12b, but caspase-8 deficient cells exhibited a further decrease in expression of these genes. Furthermore, the ability of TLR stimuli to induce optimal IκB kinase phosphorylation and nuclear translocation of the nuclear factor kappa light chain enhancer of activated B cells family member c-Rel required caspase activity. Interestingly, overexpression of c-Rel was sufficient to restore expression of IL-12 and IL-1β in caspase-8–deficient cells. Moreover,Ripk3−/−Casp8−/−mice were unable to control infection by the intracellular parasiteToxoplasma gondii, which corresponded to defects in monocyte recruitment to the peritoneal cavity, and exogenous IL-12 restored monocyte recruitment and protection of caspase-8–deficient mice during acute toxoplasmosis. These findings provide insight into how caspase-8 controls inflammatory gene expression and identify a critical role for caspase-8 in host defense against eukaryotic pathogens.

scholarly journals Analysis of cell division parameters and cell cycle gene expression during the cultivation of Arabidopsis thaliana cell suspensions

2001 ◽  
Vol 52 (361) ◽  
pp. 1625-1633 ◽  
Author(s):  
Caroline Richard ◽  
Christine Granier ◽  
Dirk Inzé ◽  
Lieven De Veylder
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Arabidopsis Thaliana ◽  
Cell Division ◽  
Cell Suspensions ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression

Plant cell suspensions resistant to and recalcitrant to cryopreservation procedures

Cryobiology ◽  
1988 ◽  
Vol 25 (6) ◽  
pp. 534
Author(s):  
F. van Iren ◽  
J. Schripsema ◽  
E.W.M. Schrijne-Makers ◽  
R. van der Heijden ◽  
R. Verpoorte ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Suspensions
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