scholarly journals Effect of adding aqueous extract of Melissa officinalis leaves and some other antioxidants to Tris extender on post-cooling and post- cryopreservative plasma membrane and acrosome integrity percentages of Holstein bulls

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
T Abdulkareem ◽  
O Alzaidi
Keyword(s):  
Plasma Membrane ◽  
Vitamin E ◽  
Vitamin C ◽  
Aqueous Extract ◽  
The Other ◽  
Melissa Officinalis ◽  
Semen Characteristics ◽  
Holstein Bulls ◽  
Time Periods ◽  
Acrosome Integrity

This study was conducted to explore the effect of adding aqueous extract of Melissa officinalis leaves (AEMOL), some antioxidants to Tris extender on sperm’s plasma membrane and acrosome integrity for Holstein bulls following different preservation periods. The study was executed at the Department of Artificial Insemination, Abu-Ghraib belong to the Directorate of Animal Resource, Ministry of Agriculture, during the period from October 2015 to February 2016 using ten Holstein bulls of 2.5-3 years old. Semen was collected via artificial vagina in one ejaculate per bull per week for the 7-week experimental period. Pooled semen was equally divided into ten groups using Tris extender. In this experiment, AEMOL (0.062mg/100 ml; A2), WEMOL (0.062mg/100 ml) + 5 mM of vitamin C (A3), AEMOL (0.062mg/100 ml + 0.08 mM of Trolox (A4), AEMOL (0.062mg/100 ml + 100 IU of catalase (A5),100 IU of catalase (A6), 5 mM of vitamin C (A7), 0.08 mM of Trolox (A8), 0.2 mM of vitamin E (A9) and AEMOL (0.062mg/100 ml +0.2 mM of vitamin E (A10) and comparisons in response were made with the control group (Tris extender, A1). The effect of these additives on semen characteristics of Holstein bulls for different preservation periods (cooling at 5◦C, 48 h, 1, 2 and 3 months post cryopreservation, PC) were studied. The A2 group exhibited greater (P≤0.01) plasma membrane and acrosome integrity percentage as compared with A1 group at all preservation time periods. Greater (P≤ 0.01) plasma membrane and acrosome integrity percentage were noticed in comparison with the A1 group at all preservation time periods. On the other hand, A5 group exhibited greater (P≤ 0.01) plasma membrane and acrosome integrity percentage, as well as, Adding of vitamin E alone (A9) or combined with AEMOL (A10) to Tris extender had positive (P≤ 0.01) effect in improving all the semen characteristics at all preservation time periods. In conclusion, adding of AEMOL as alone or combined with the other synthetic antioxidants to Tris extenders had a crucial role in improving PC sperm's plasma membrane and acrosome integrity percentage of Holstein bulls. This was reflected positively in increasing pregnancy rates of the inseminated cows and owner's income consequently.

scholarly journals Effect of adding aqueous extract of Melissa officinalis leaves and some other antioxidants to milk–based extender on post-cooling and post- cryopreservative sperm’s individual motility and live sperm percentage of Holstein bulls

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
T Abdulkareem ◽  
O Alzaidi
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Aqueous Extract ◽  
Experimental Period ◽  
The Other ◽  
Melissa Officinalis ◽  
Holstein Bulls ◽  
Time Periods ◽  
Animal Resource ◽  
Live Sperm

This study was conducted to explore the effect of adding aqueous extract of Melissa officinalis leaves (AEMOL), some antioxidants and their combinations to the milk-based extenderfor increase on sperm's cells individual motility percentage on and live sperms percentage semen for Holstein bulls following different preservation periods. The study was executed at the Department of Artificial Insemination, Abu-Ghraib belong to the Directorate of Animal Resource, Ministry of Agriculture, during the period from October 2015 to February 2016 using ten Holstein bulls of 2.5-3 years old. Semen was collected via artificial vagina in one ejaculate per bull per week for the 7-week experimental period. Pooled semen was equally divided into ten groups within one experiment. In this experiment, AEMOL (0.062mg/100 ml; A2), WEMOL (0.031 mM) + 5 mM of vitamin C (A3), AEMOL (0.062mg/100 ml + 0.08 mM of Trolox (A4),AEMOL (0.062mg/100 ml + 100 IU of catalase (A5),100 IU of catalase (A6), 5 mM of vitamin C (A7), 0.08 mM of Trolox (A8), 0.2 mM of vitamin E (A9) and AEMOL (0.062mg/100 ml +0.2 mM of vitamin E (A10). The effect of these additives on semen characteristics of Holstein bulls for different preservation periods (cooling at 5◦C, 48 hrs., 1, 2 and 3 months post cryopreservation, PC) were studied. The A2 group exhibited greater (P≤ 0.01) sperm's cell individual motility, live sperm percentage, as compared with A1 group at all preservation periods. Greater sperm's cells individual motility, live sperm percentage noticed in A4 group in comparison with the A1 group at all preservation time periods. On the other hand, A5 group exhibited greater (P≤ 0.01) sperm's cell individual motility, live sperm percentage, as well as, adding of vitamin E alone (A9) or combined with AEMOL (A10) to milk-based extender had a positive (P≤ 0.01) effect in improving sperm's cell individual motility and live sperm percentage at all preservation time periods. In conclusion, adding of AEMOL as alone or combined with the other synthetic antioxidants to milk-based extenders had a crucial role in improving PC sperm's cell individual motility and live sperm percentage of Holstein bulls. This was reflected positively on increasing pregnancy rates of the inseminated cows.

scholarly journals EFFECT OF ADDING PENTOXIFYLLINE AND NITRIC OXIDE TO TRIS EXTENDER ON SOME POST-CRYOPRESERVED SEMEN ATTRIBUTES OF HOLSTEIN BULLS

2020 ◽  
Vol 51 (2) ◽  
pp. 619-628
Author(s):  
Mohammed & et al.
Keyword(s):  
Nitric Oxide ◽  
Plasma Membrane ◽  
Membrane Integrity ◽  
Control Group ◽  
Plasma Membrane Integrity ◽  
Abnormal Sperm ◽  
Semen Characteristics ◽  
Holstein Bulls ◽  
Time Periods ◽  
Live Sperm

This study was aimed to explore the effect of adding pentoxifylline (PTX) and nitric oxide (NO) to Tris extender on some post-cryopreserved semen attributes of Holstein bulls for different preservation periods (cooling at 5◦C, 48 hrs., 1, 2 and 3 months post cryopreservation, PC). Seven Holstein bulls of 2.5-3 years old were used in the current study during the period from 20th November, 2017 to 20th August, 2018. Pooled semen was equally divided into three groups within one experiment. PTX (0.192 g / 100 ml extender) and NO (0.018 g / 100 ml extender) were added to Tris extender and comparisons in response were made with the control group (Tris extender, C). The PTX group exhibited greater (P≤ 0.01) sperm's cell individual motility percentage as compared with the C group at cooling (5ºc) and 48 hr PC periods, while, PTX and NO groups were superior in these percentages at the remaining PC periods than C group. Excluding data of 2 months PC, greater (P≤ 0.01) live sperm percentage was observed in PTX and NO groups in comparison with the C group at all preservation periods. Lesser (P≤ 0.01) abnormal sperm percentage were noticed for PTX and NO groups as compared with the C group at all preservation time periods. The PTX and NO groups exhibited greater   (P≤ 0.01) acrosome and plasma membrane integrity percentages in comparison with the C group at all preservation time periods. In conclusion, adding PTX and NO to Tris extender enhanced some of  PC semen characteristics of Holstein bulls at different preservation periods.

The protective effect of vitamin C, vitamin E and selenium combination therapy on ethanol-induced duodenal mucosal injury

2004 ◽  
Vol 23 (8) ◽  
pp. 391-398 ◽  
Author(s):  
M Koyuturk ◽  
S Bolkent ◽  
S Ozdil ◽  
S Arbak ◽  
R Yanardag
Keyword(s):  
Nitric Oxide ◽  
Vitamin E ◽  
Nitric Oxide Synthase ◽  
Vitamin C ◽  
Protective Effect ◽  
Neuronal Nitric Oxide Synthase ◽  
Mucosal Injury ◽  
The Other ◽  
Absolute Ethanol ◽  
Oxide Synthase

In this study, the effect of a combination of vitamin C, vitamin E and selenium on ethanol-induced duodenal mucosal damage in rats was investigated morphologi-cally and biochemically. The duodenal mucosal injury was produced by oral administration of 1 mL of absolute ethanol to each rat. Animals received vitamin C (250 mg/kg), vitamin E (250 mg/kg) and selenium (0.5 mg/kg) for 3 days and absolute ethanol 1 hour after last antioxidant administration and were sacrificed 1 hour after absolute ethanol. Extreme degeneration in intestinal mucosa of rats given ethanol was observed morphologically. In addition, an increase in neuronal nitric oxide synthase immunoreactive areas was observed in the rats of the group given ethanol. On the other hand, a normal morphological appearance and a decrease in neuronal nitric oxide synthase immunoreactive areas were detected in the rats given ethanol+vitamin C+vitamin E+selenium. In the group to which ethanol was administered, an increase in serum cholesterol and a decrease in serum albumin levels were determined. On the other hand, in the group to which ethanol+vitamin C+vitamin E+selenium were administered, serum cholesterol value decreased, and the serum albumin level increased. As a result, we can say that the combination of vitamin C, vitamin E and selenium has a protective effect on ethanolinduced duodenal mucosal injury.

scholarly journals Effect of Vitamins and Amino Acids as Cryoprotectant Solution Supplementation for the Cryopreservation of Tambaqui Semen (Colossoma macropomum)

2018 ◽  
Vol 46 (1) ◽  
pp. 8 ◽  
Author(s):  
Júlia Trugilio Lopes ◽  
Mayara Setúbal Oliveira-Araújo ◽  
Renata Vieira Do Nascimento ◽  
Yasmin Maia Ferreira ◽  
Assis Rubens Montenegro ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Amino Acids ◽  
Vitamin E ◽  
Vitamin C ◽  
The Other ◽  
Control Treatment ◽  
Oxygen Species ◽  
Colossoma Macropomum ◽  
Progressive Motility ◽  
Cryoprotective Solution

Background: The addition of antioxidant substances to a cryoprotective solution can increase its protective capacity, shielding spermatozoa from the oxidative stress caused by the seminal cryopreservation process. However, there is no record of a seminal cryopreservation protocol of tambaqui (Colossoma macropomum) using antioxidants as a supplement to the cryoprotective solution. Thus, the objective of this study was to evaluate the effects of adding vitamin C, vitamin E, cysteine, and/or taurine to the seminal cryopreservation of tambaqui.Materials, Methods & Results: Pools of semen (n = 10) were diluted in cryoprotective solutions supplemented with: vitamin C (T1), vitamin E (T2), vitamin C + vitamin E (T3), cysteine (T4), taurine (T5), and taurine + cysteine (T6). The control treatment (T7) was not supplemented. Diluted semen was loaded in 0.5 mL straws, frozen in a dry-shipper, stored in a cryogenic cylinder, and then thawed in a water bath (45ºC for eight seconds). The quality of fresh and cryopreserved semen was evaluated by measuring total motility, progressive motility, curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity, and straightness using a computerized system of sperm analysis. Sperm membrane integrity parameters were analyzed using eosin-nigrosin staining, sperm morphology was assessed using pink bengal staining, and motility duration was measured by a digital timer. Data were analyzed using the statistical program SAS (2002) and the results were expressed as means ± standard error of the mean. The results showed that, in general, there was no significant increase in seminal quality when antioxidants were added to the cryoprotective solution. The T5 treatment promoted an increase (P < 0.05) in progressive motility when compared to T1 (6.33 ± 1.14% and 2.98 ± 0.88%, respectively). However, it did not differ significantly (P > 0.05) from the other treatments. Treatments T2 and T5 presented the highest values of VCL (34.74 ± 2.58 and 33.60 ± 1.81 μm.s-1, respectively). These were higher (P < 0.05) than T1 (26.31 ± 1.64 μm.s-1) but not different (P > 0.05) from the T7 control (30.87 ± 1.49 μm.s-1). The VSL and VAP results showed that T1 presented the lowest velocity (9.89 ± 1.75 and 15.06 ± 1.92 μm.s-1, respectively) compared to the other treatments (P < 0.05) that did not differ from each other. Combining the two vitamins (T3) or the two amino acids (T6) was not advantageous in relation to the use of only one of these antioxidants.Discussion: The present study reports, for the first time, results of the addition of antioxidants to the tambaqui seminal freezing medium. The addition of taurine and vitamin E, although not significantly different from the control treatment, resulted in a tendency to increase sperm kinetics. This effect may be due to the action of taurine as a regulator of Ca2+ transporters, which is necessary to trigger sperm activation, and to the ability of vitamin E to scavenge reactive oxygen species produced during lipid peroxidation. On the other hand, the reduced sperm quality observed when vitamin C was used may have been related to the toxicity caused by a high concentration of this vitamin. In addition, once the safe dose of antioxidants has been exceeded, the normal physiological functions of reactive oxygen species can be inhibited. Thus, it is concluded that the use of vitamin E and taurine promotes promising results of curvilinear velocity after thawing of sperm. Therefore, these treatments are recommended, as well as more tests to determine their optimal concentrations.

108 EFFECT OF DIMETHYLFORMAMIDE AND METHYLFORMAMIDE ON OXIDATIVE STATUS OF MANGALARGA STALLIONS CRYOPRESERVED SEMEN

2010 ◽  
Vol 22 (1) ◽  
pp. 213
Author(s):  
F. A. Oliveira Neto ◽  
M. Nichi ◽  
E. G. A. Perez ◽  
J. R. C. Gurgel ◽  
G. H. Ferreira ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Plasma Membrane ◽  
Membrane Integrity ◽  
The Other ◽  
Lipid Peroxidation Product ◽  
Oxygen Species ◽  
Mitochondrial Potential ◽  
Plasma Membrane Integrity ◽  
Peroxidation Product ◽  
Acrosome Integrity

Cryopreservation of equine semen has been widely studied by several research groups because of the large breed and individual variation in sperm freezability. A key factor in sperm cryopreservation is the high incidence of oxidative stress, an imbalance between reactive oxygen species (ROS) and antioxidant protection, which impairs sperm functionality by attacking plasma membrane, acrosome, mitochondria, and DNA. In order to study the resistance of equine spermatozoa to different reactive oxygen species (ROS), sperm samples from 4 Mangalarga stallions were collected using an artificial vagina. Samples were cryopreserved in extenders containing dimethylformamide (DMF) or methylformamide (MF). After thawing and washing, sperm samples were then incubated (1 h, 37°C) with 4 ROS inducer mechanisms: xanthine/xanthine oxidase (produces superoxide anion), hydrogen peroxide (4 mM), ascorbate/ferrous sulfate (4 mM; produced hidroxyl radical), and malondialdehyde (MDA, lipid peroxidation product). Samples were evaluated using the 3-3′ diamino benzidine (DAB) stain, as an indicator of mitochondrial activity; the eosin nigrosin staining, to evaluate plasma membrane integrity; the simple stain (fast green/Bengal rose), to assess acrosome integrity; and the measurement of thiobarbituric acid reactive substances (TBARS), a lipid peroxidation product. Statistical analysis was performed using the Student t-test and LSD test. Results showed that sperm mitochondrial potential of frozen-thawed samples in MF was highly susceptible to the attack of hydroxyl radical and hydrogen peroxide. No effect of ROS was observed on membrane and acrosome integrity. On the other hand, samples cryopreserved in DMF showed no differences in susceptibility to ROS. When evaluating the main effects of different extenders, results showed a higher protective effect of the MF extender on acrosome integrity and mitochondrial potential (MF: 12.1 ± 2.2 and 7.8 ± 2.3% v. DMF: 3.4 ± 0.7 and 1.1 ± 0.7%, respectively, P < 0.05). However, a negative effect of MF extender was observed regarding the percentage of sperm showing intact membrane and TBARS content (MF: 2.0 ± 0.8% and 517 ± 115 ng/106 sperm v. DMF: 20.6 ± 1.7% and 118 ± 44 ng/106 sperm, respectively, P < 0.05). A strong negative correlation was found between TBARS and plasma membrane integrity (r = -0.88; P = 0.004) for samples cryopreserved in DMF, whereas a positive correlation was found between TBARS and sperm with full mitochondrial potential (r = 0.73; P = 0.04). Results of the present study indicate that DMF may play a role in the protection of sperm against the attack of ROS. However, such action is apparently limited to the plasma membrane. On the other hand, the MF-supplemented extender exerts an intracellular protection. Therefore, the antioxidant therapy, especially hydrogen peroxide and hydroxyl radical scavengers, may be an alternative to improve the post-thaw quality of MF-supplemented cryopreserved semen in stallions, by increasing extracellular antioxidant capacity. The authors thank Nutricell for financial support and the media used in the present experiment.

scholarly journals EFFECT OF SWIM-UP AND GLASS WOOL TECHNIQUES, WITH ADDING ANTIOXIDANTS TO TRIS EXTENDER ON IMPROVING POST-CRYOPRESERVED TOTAL SPERM CHARACTERISTICS IN STRAW AND FREEZABILITY PERCENTAGE FOR LOW SEMEN QUALITY OF HOLSTEIN BULLS

2021 ◽  
Vol 52 (4) ◽  
pp. 885-895
Author(s):  
Hassan & et al.
Keyword(s):  
Superoxide Dismutase ◽  
Plasma Membrane ◽  
Vitamin E ◽  
Semen Quality ◽  
Glass Wool ◽  
Normal Morphology ◽  
Sperm Characteristics ◽  
Holstein Bulls ◽  
Sperm Separation

This study was conducted to investigate the effect of swim-up and glass wool as sperm separation techniques with adding vitamin E and superoxide dismutase (SOD) to Tris extender on improving post-cryopreserved total sperm characteristics in straw and freezability percentage for low semen quality of Holstein bulls. Low and good of semen were extended using Tris extender. Good semen quality (GSQ) was divided into 3 groups (L1; Tris extender, L2; 2 mM vitamin E, L3; 200 IU SOD) Low semen quality (LSQ) was divided into two main groups, and subdivided into 3 sub-groups (L4; Tris extender, L5; 2 mM vitamin E, L6; 200 IU SOD). In the second main group, swim-up and glass wool techniques were used with adding vitamin E and SOD and subdivided into 3 sub-groups with each technique, and referred to L7, L8 and L9 for swim-up technique and L10, L11 and L12 for glass wool technique. Sperms with good motile intact acrosome and plasma membrane normal morphology of sperm were obtained using the glass wool separation technique with adding vitamin E.

Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender

2017 ◽  
Author(s):  
T.K. S. Rao ◽  
T. K. Mohanty ◽  
M. Bhakat
Keyword(s):  
Quality Improvement ◽  
Vitamin E ◽  
Vitamin C ◽  
Dose Rate ◽  
Semen Quality ◽  
Poor Quality ◽  
Bull Semen ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
Vitamin E And C

Present study was aimed to improve poor quality crossbred bulls semen, as occurrence of poor quality ejaculates are one of the major problems in crossbred bulls. Antioxidants and its combination was tried to overcome such problems. Vitamin E, Vitamin C and Vitamin E+C were supplemented at dose rate of 1mg, 5 mM and 1mg + 5mM per ml respectively in split ejaculates to evaluate semen quality during preservation at refrigerated temperature and cryopreservation. The results showed that semen characteristics were better in antioxidant supplemented ejaculates. Total sperm abnormality were significantly lower (p>0.05) in antioxidant supplemented group. The seminal characteristics of crossbred bull semen showed significantly better (p>0.05) performance on preservation, when fortified with Vitamin E as compared to Vitamin C alone as well as Vitamin E and C in combination. The performance of semen additives, were supportive to semen characteristics especially for Vitamin E, moreover fortification with Vitamin E+C was slightly superior to Vitamin C. It can be concluded that fortification of Vitamin E has beneficial role in semen quality improvement followed by Vitamin E+C and Vitamin C.

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