AbstractObjectiveTo evaluate a web-based nutritional knowledge questionnaire for primary-school children.DesignChildren's nutritional knowledge was assessed in five domains: healthy choices (twenty-seven items), estimated recommended portions/servings (eight items), nutrient content (five items), main food function (five items) and categorization of food items (eight items).SettingThe questionnaires were completed in school.SubjectsA convenience sample of 576 Belgian children (aged 7–12 years) from fourteen primary schools completed the questionnaire once, 386 completed the questionnaire twice.ResultsHealthy choices could be answered correctly by 73 % of the children, nutrients by 59 %, food categorization by 49 %, main function by 38 % and portion estimation by 36 %. Children's test–retest intra-class correlations were 0·75 for healthy choices, 0·33 for nutrients, 0·61 for food categorization, 0·44 for main function, 0·47 for portion estimation and 0·76 for the total scale. The intra-class correlation was lower in the youngest age group (grade 2: 0·51, grade 4: 0·65, grade 6: 0·66). The total score was significantly lower in the retest. The instrument was in general positively evaluated by the children.ConclusionsThe instrument is a promising, practical, inexpensive tool with acceptable test–retest reliability in fourth and sixth graders.