Isolation and Characterization of Bifidobacteria from diverse ecosystems

2019 ◽  
Vol 8 (2) ◽  
pp. 43-48
Author(s):  
Halima Aurass Bahloul ◽  
Hadadji Miloud ◽  
Mebrouk Kihal
Keyword(s):  
Newborn Infants ◽  
Food Samples ◽  
High Concentration ◽  
Dominant Group ◽  
Human Gut ◽  
Important Place ◽  
Isolation And Characterization ◽  
Probiotic Strains ◽  
Commercial Milk

Bifidobacteria represent the largest group of human intes nal bacteria. They have an important place in human health and represent the dominant group of micro ora. For this reason, they are key members of the human gut microbiota, particularly during the first one to two years following birth. The aim of this study was to test the sensibility of Bifidobacterium isolated from some strains at different concentrations of salinity and high concentration of bile as a criterion to select probiotic strains. Following the behavior of strains of Bifidobacteria isolated from different ecosystems was considered in order to develop the therapeu c aliment food . Samples from newborn infants aged 3 months, bee intestine, fermented commercial milk and yoghurt of bifidus containing Bifidobacteria was used and the isolated strains belonged to -breve and -longum species. The results of resisitance of our species of Bifidobacteria demonstrated a total inibition of croissance at 5.5% of NaCl but in 5% media of NaCl we observed a moderate croissance at di erent me of kinetics croissance .

Isolation and characterization of lignocellulosic fiber from jute bast by the organic solvent degumming system: an alkali-free method

2021 ◽  
pp. 004051752110154
Author(s):  
Zhihui Qin ◽  
Shuyuan Zhao ◽  
Liu Liu ◽  
Zhaohe Shi ◽  
Longdi Cheng ◽  
...  
Keyword(s):  
National Standards ◽  
X Ray Diffraction ◽  
High Concentration ◽  
X Ray ◽  
Lignocellulosic Fibers ◽  
Lignocellulosic Fiber ◽  
Isolation And Characterization ◽  
Alkali Consumption ◽  
Scanning Electron

Degumming is the dominant method for insolating lignocellulosic fibers in textile applications. Traditional alkaline degumming (TAL), as a common method, requires a high-concentration alkali and has been a severe challenge to the environment. In the research reported here, the possibility of innovative jute degumming by organic solvents 1-2 propylene glycol and a combination of additive green oxygen (GO-OS) was studied. The results revealed that fibers could be extracted by this system (under condition of 0.9% GO-OS, 180°C, 120 min), and obtained fibers with higher breaking tenacity (7.1 cN/dtex), yield (65.7%), breaking elongation (2.87%) and residual gum (11.7%), which all meet the requirement of the relevant Chinese Textile National Standards. Notably, the required reaction time (120 min) of the GO-OS system was 180 min shorter than that of the TAL method. Furthermore, the modifications introduced by the degumming effect on physicochemical aspects were characterized and confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. This study provides a promising degumming method for separating jute lignocellulose without acid and alkali consumption.

THE ALKALOIDS OF FUMARIACEOUS PLANTS: VIII. CORYDALIS AUREA, WILLD. AND THE CONSTITUTION OF BICUCINE

1933 ◽  
Vol 9 (5) ◽  
pp. 436-442 ◽  
Author(s):  
R. H. F. Manske
Keyword(s):  
Empirical Formula ◽  
Hydroxy Acid ◽  
Phenolic Hydroxyl ◽  
High Concentration ◽  
Chemical Examination ◽  
Low Concentration ◽  
Isolation And Characterization ◽  
Stems And Leaves ◽  
Constitutional Analysis

The chemical examination of the alkaloids of Corydalis aurea has shown an unusual complexity and of the total of more than ten alkaloids thus far isolated only six are now described. The record deals chiefly with the stems and leaves of the plant in which protopine was present in exceptionally low concentration (0.025%). Equally exceptional is its high concentration m the roots (1.6%). l-Tetrahydropalmatine constituted the largest fraction of the remaining alkaloids, and its present isolation is the first on record although the d-form was previously known. Two new and well-characterized alkaloids, which have been named capaurine and capauridine, respectively, are isomeric and are best represented by the empirical formula C21H27O5N. Both contain one phenolic hydroxyl and four methoxyl groups, and yield on methylation non-phenolic bases which do not appear to be identical. The presence of two bases, bicuculline and bicucine, which were first recorded in this series of papers, has again been demonstrated.The constitutional analysis of the new bases, as well as the isolation and characterization of the minor alkaloids, is in progress.In an appended note it is shown that bicuculline and bicucine are closely related and interconvertible. The latter is the free γ-hydroxy acid of which the former is the lactone.

SUDAY ON A SUBSTANCE FUNCTIONALLY LIKE PROTEIN C IN PLASMA

1987 ◽  
Author(s):  
I TSUNEIZUMI ◽  
T MEGURO ◽  
K YAMADA
Keyword(s):  
Molecular Weight ◽  
Protein C ◽  
Present Report ◽  
Newborn Infants ◽  
Exchange Chromatography ◽  
Isolation And Characterization ◽  
K Deficiency ◽  
Clinically Significant ◽  
Aluminium Hydroxide Gel

The present report first deals with the isolation and characterization of a substance found to demonstrate protein C like activity(PCLA). When it was activated by thrombin, the PCLA substance prolonged the activated partial thromboplastin time(aPTT). The activated PCLA substance also hydrolyzed synthetic substrates such as S�2238, S�2366 and S�2266,while the PCLA substance was not cross reacted with anti-protein C serum(Behring Manheim). The PCLA substance was adsorbed by both aluminium hydroxide gel and barium sulfate. The level of Km, optimum pH and optimum I.S. in the amido-lytic reaction with synthetic substrate is shown in the table.In the chromatography of Sepharose CL-6B gel, the PCLA substance was eluted in the fraction of higher molecular weight, while protein C was eluted with a lower fraction.This prompts an estimate that the molecular weight of the PCLA substance is approximately 200,000. Through DEAE-Sepharose CL-6B gel ion exchange chromatography, the PCLA substance was eluted by a lower ionic strength than was protein C.The levels of a PCLA substance were low in the patients with a vitamin K deficiency and in newborn infants. It is expected that our findings regarding the PCLA substance will be as clinically significant as that which we know about protein C at present.

Isolation and characterization of Rhodococcus sp. NB5 capable of degrading a high concentration of nitrobenzene

2011 ◽  
Vol 51 (4) ◽  
pp. 397-403 ◽  
Author(s):  
Hui Lin ◽  
Xue-jiao Chen ◽  
Hai-tao Ding ◽  
Xiao-ming Jia ◽  
Yu-hua Zhao
Keyword(s):  
High Concentration ◽  
Isolation And Characterization ◽  
Rhodococcus Sp

Isolation and characterization of small Colony Variants of Staphylococcus aureus in Various Food Samples

2021 ◽  
pp. 102097
Author(s):  
Malik Wajid Hussain Chan ◽  
Zulfiqar Ali Mirani ◽  
Muhammad Naseem Khan ◽  
Amjad Ali ◽  
Abdul Basit Khan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Food Samples ◽  
Small Colony Variants ◽  
Isolation And Characterization ◽  
Small Colony

scholarly journals Isolation and characterization of a novelArabidopsis thatianamutant that requires a high concentration of boron

2004 ◽  
Vol 50 (7) ◽  
pp. 1183-1185
Author(s):  
Noriaki Aoki ◽  
Kyotaro Noguchi ◽  
Hiroaki Hayashi ◽  
Toru Fujiwara
Keyword(s):  
High Concentration ◽  
Isolation And Characterization

scholarly journals An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies

2017 ◽  
Vol 33 (S1) ◽  
pp. S-31-S-39 ◽  
Author(s):  
Martin Trapecar ◽  
Shahzada Khan ◽  
Nadia R. Roan ◽  
Tsui-Hua Chen ◽  
Sushama Telwatte ◽  
...  
Keyword(s):  
Human Gut ◽  
Isolation And Characterization

Corrigendum to “Isolation and characterization of probiotic strains for improving oral health” [Arch. Oral Biol. 57 (2012) 539-549]

2013 ◽  
Vol 58 (5) ◽  
pp. 558-559 ◽  
Author(s):  
Montserrat Bosch ◽  
José Nart ◽  
Sergi Audivert ◽  
Miguel Angel Bonachera ◽  
Antonio Santos Alemany ◽  
...  
Keyword(s):  
Oral Health ◽  
Isolation And Characterization ◽  
Oral Biol ◽  
Probiotic Strains
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