Single-dose Intramuscular Injection Toxicology of Danggui Pharmacopuncture (DGP) in Sprague-Dawley Rats

Abstract Background: Natural products such as mushrooms are increasingly used as adjunct therapies in cancer to stimulate the immune system. However, little is known about their interaction with chemotherapy drugs. This study investigated whether a commercial polysaccharide peptide (PSP) extract of the mushroom Coriolus (C.) versicolor interacts with tamoxifen, a common chemotherapy drug used in breast cancer. Methods: The pharmacokinetic and biochemical parameters of tamoxifen in female Sprague-Dawley rats’ serum were determined after orally administering tamoxifen (20 mg/kg) at a single dose and repeated dosing with and without C. versicolor (340 mg/kg). Results: Although the area-under-curve and maximum concentration showed no significant differences in both the single dose and repeated dosing, time to reach maximum concentration (Tmax) increased by 228% and 93%, respectively, in the rats treated with C. versicolor (p<0.05). The repeated dosing of C. versicolor, when co-administered with repeated dosing of tamoxifen, maintained 19 out of 23 biochemical serum parameters in rats compared to control (p<0.05). Using hepatic female rat microsomes, an in vitro study showed that the extract (10 – 100 µg/mL) did not significantly alter the rate of tamoxifen depletion which is known to be mediated by cytochromes P450 (CYP450). Conclusions: These results indicate that C. versicolor may delay the intestinal absorption of tamoxifen and maintain biochemical serum parameters, without any change in tamoxifen’s metabolism. If clinical trials confirm this result, the timing of tamoxifen and C. versicolor administration would need to be considered to avoid potential drug interactions resulting from a delay in achieving the systemic level of the anti-cancer medication.

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Single-dose Intravenous Toxicology Testing of Daebohwalryeok Pharmcopuncture in Sprague-Dawley Rats

Journal of Pharmacopuncture ◽

10.3831/kpi.2015.18.014 ◽

2015 ◽

Vol 18 (2) ◽

pp. 42-50

Author(s):

Ki-Rok Kwon ◽

Seung-Ho Sun ◽

Sunju Park ◽

Jong-Jin Jeong ◽

Kwang-Ho Lee ◽

...

Keyword(s):

Single Dose ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Intravenous Single-dose Toxicity of Mountain Ginseng Pharmacopuncture in Sprague-Dawley Rats

Journal of Pharmacopuncture ◽

10.3831/kpi.2014.17.026 ◽

2014 ◽

Vol 17 (3) ◽

pp. 50-56 ◽

Cited By ~ 1

Author(s):

Kwangho Lee ◽

Seungho Sun ◽

Junsang Yu ◽

Chungsan Lim ◽

Kirok Kwon

Keyword(s):

Single Dose ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Pharmacokinetics of All-trans Retinoyl beta-Glucuronide in Rats following Intraperitoneal and Oral Administration

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.73.4.251 ◽

2003 ◽

Vol 73 (4) ◽

pp. 251-257 ◽

Cited By ~ 3

Author(s):

Romans ◽

Barua ◽

Olson

Keyword(s):

High Performance Liquid Chromatography ◽

Single Dose ◽

High Performance ◽

Corn Oil ◽

Major Product ◽

Plasma Samples ◽

Sprague Dawley ◽

Blood Samples ◽

Sprague Dawley Rats ◽

Plasma Retinol

The purpose of this study was to examine the pharmacokinetics of a single dose (6.3 mumol, 3 mg) of all-trans retinoyl beta-glucuronide (RAG), when given either orally in corn oil or by intraperitoneal (IP) injection in dimethylsulfoxide (DMSO) to adult Sprague-Dawley rats. Following dosing, serial blood samples were collected at various times up to 48 hours from each rat via saphenous vein puncture. Retinoids were extracted from plasma samples and analyzed by high-performance liquid chromatography. In the plasma of IP-dosed rats (n = 6), a derivative of RAG, tentatively identified as the lactone of RAG (RAGL), was the major product found. RAGL persisted in the plasma for up to 48 hours. Much smaller concentrations of RAG and of retinoic acid (RA) were also present in the plasma at two to four hours, but generally not thereafter. In orally dosed rats (n = 6), neither RAG nor its products, except for occasional traces of the lactone, were detected. Plasma retinol levels decreased in both IP-injected and orally treated rats, the decrease being significant in orally dosed rats.

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Antiserum against tumor necrosis factor enhances lipopolysaccharide fever in rats

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1990.258.2.r332 ◽

1990 ◽

Vol 258 (2) ◽

pp. R332-R337 ◽

Cited By ~ 11

Author(s):

N. C. Long ◽

S. L. Kunkel ◽

A. J. Vander ◽

M. J. Kluger

Keyword(s):

Tumor Necrosis Factor ◽

Intramuscular Injection ◽

Tumor Necrosis ◽

Rabbit Serum ◽

Normal Rabbit Serum ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Control Serum ◽

Necrosis Factor

The role of tumor necrosis factor (TNF, cachectin), a putative endogenous pyrogen, was investigated by comparing fever and plasma TNF levels after the intraperitoneal and intramuscular injection of 10 micrograms/kg lipopolysaccharide (LPS) into male Sprague-Dawley rats and by neutralization of endogenous TNF using TNF antiserum. An intraperitoneal injection of LPS caused a biphasic fever that lasted approximately 6.5 h. TNF levels in these rats peaked at 657 +/- 222 U/ml at 1 h then declined to virtually undetectable levels by the fourth hour. The intramuscularly injected animals showed a lower monophasic fever and low sustained TNF levels (40 +/- 10 U/ml at 1 h, 18 +/- 11 U/ml at 4 h). In a second study, an antiserum that had been shown to neutralize rat TNF was injected intraperitoneally 2 h before the intramuscular injection of 10 micrograms/kg LPS. Control rats were injected with normal rabbit serum before LPS. During the second hour after the injection of LPS, the animals that received the antiserum developed fevers that tended to be lower than those seen in the rats that were injected with control serum (0.33 +/- 0.06 vs. 0.58 +/- 0.1), although this difference was not significant. However, during the third through eighth hours after LPS, the antiserum-injected rats had mean body temperatures that were significantly higher than those of the control rats (1.62 +/- 0.11 vs. 1.07 +/- 0.09; P = 0.0005).(ABSTRACT TRUNCATED AT 250 WORDS)

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Pharmacokinetics Interaction between Imatinib and Genistein in Rats

BioMed Research International ◽

10.1155/2015/368976 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Zhe Wang ◽

Li Wang ◽

Meng-ming Xia ◽

Wei Sun ◽

Cheng-ke Huang ◽

...

Keyword(s):

Single Dose ◽

Multiple Dose ◽

Pharmacokinetic Parameters ◽

Control Group ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Significant Difference ◽

Group A ◽

Group B ◽

Group Control Group

The objective of this work was to investigate the effect of orally administered genistein on the pharmacokinetics of imatinib and N-desmethyl imatinib in rats. Twenty-five healthy male SD (Sprague-Dawley) rats were randomly divided into five groups: A group (control group), B group (multiple dose of 100 mg/kg genistein for consecutive 15 days), C group (multiple dose of 50 mg/kg genistein for consecutive 15 days), D group (a single dose of 100 mg/kg genistein), and E group (a single dose of 50 mg/kg genistein). A single dose of imatinib is administered orally 30 min after administration of genistein (100 mg/kg or 50 mg/kg). The pharmacokinetic parameters of imatinib and N-desmethyl imatinib were calculated by DAS 3.0 software. The multiple dose of 100 mg/kg or 50 mg/kg genistein significantly (P<0.05) decreased theAUC0-tandCmaxof imatinib.AUC0-tand theCmaxof N-desmethyl imatinib were also increased, but without any significant difference. However, the single dose of 100 mg/kg or 50 mg/kg genistein has no effect on the pharmacokinetics of imatinib and N-desmethyl imatinib. Those results indicated that multiple dose of genistein (100 mg/kg or 50 mg/kg) induces the metabolism of imatinib, while single dose of genistein has no effect.

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Single dose toxicity study of IRDye 800CW in Sprague-Dawley rats

10.1117/12.852783 ◽

2010 ◽

Cited By ~ 1

Author(s):

Milton V. Marshall ◽

Daniel Draney ◽

Eva M. Sevick-Muraca ◽

D. Michael Olive

Keyword(s):

Single Dose ◽

Toxicity Study ◽

Sprague Dawley ◽

Sprague Dawley Rats

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