The effectiveness of tetragonola honey combinations Aff.biroi and royal jelly as immunomodulators : immunomodulators modelling in facing the plague of COVID-19

COVID-19 epidemic caused serious concern around the world. Innate and adaptive immunity prevents occurring acute infection. Immunomodulatory activity tests and increased leucocyte cells in rat have been carried out using honey (H) and royal jelly (RJ). This study aims to prove the combination of honey and royal jelly in increasing the leucocyte profile in male Sprague Dawley rats. This study used 28 rats divided into 7 groups, namely the negative control and positive, single dose group H and single dose RJ and the combination dose 1, dose 2, and dose 3, then on day the 8th and 11th rats were injected intraperitoneally with 2% sheep red blood cell antigen as much as 0.5 ml. Leucocyte profile were carried out on the 8th and 15th days. The results showed significant difference on leukocyte count (p = 0.000), but there was a decrease in the normal range on day 15th. The highest leucocyte count was showed on the honey and the combination 3 group. Leukocyte differential showed no significant difference (p> 0.05). The honey group and the dose combination 3 showed an increase in the number of segment neutrophils. The difference in lymphocyte reduction was seen between the honey and royal jelly group, with the dose combination 3, respectively. There are significant differences in granulocyte cells enhancement between the honey group and the dose combination 3. We concluded that single dose honey can be an immunomodulatory. Dose combination 3 can be an alternative for further research for immunomodulatory supplement product.

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Pharmacokinetics Interaction between Imatinib and Genistein in Rats

BioMed Research International ◽

10.1155/2015/368976 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Zhe Wang ◽

Li Wang ◽

Meng-ming Xia ◽

Wei Sun ◽

Cheng-ke Huang ◽

...

Keyword(s):

Single Dose ◽

Multiple Dose ◽

Pharmacokinetic Parameters ◽

Control Group ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Significant Difference ◽

Group A ◽

Group B ◽

Group Control Group

The objective of this work was to investigate the effect of orally administered genistein on the pharmacokinetics of imatinib and N-desmethyl imatinib in rats. Twenty-five healthy male SD (Sprague-Dawley) rats were randomly divided into five groups: A group (control group), B group (multiple dose of 100 mg/kg genistein for consecutive 15 days), C group (multiple dose of 50 mg/kg genistein for consecutive 15 days), D group (a single dose of 100 mg/kg genistein), and E group (a single dose of 50 mg/kg genistein). A single dose of imatinib is administered orally 30 min after administration of genistein (100 mg/kg or 50 mg/kg). The pharmacokinetic parameters of imatinib and N-desmethyl imatinib were calculated by DAS 3.0 software. The multiple dose of 100 mg/kg or 50 mg/kg genistein significantly (P<0.05) decreased theAUC0-tandCmaxof imatinib.AUC0-tand theCmaxof N-desmethyl imatinib were also increased, but without any significant difference. However, the single dose of 100 mg/kg or 50 mg/kg genistein has no effect on the pharmacokinetics of imatinib and N-desmethyl imatinib. Those results indicated that multiple dose of genistein (100 mg/kg or 50 mg/kg) induces the metabolism of imatinib, while single dose of genistein has no effect.

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Ratio Kadar Ldl/Hdl Tikus Putih Sprague Dawley Hiperlipidemia Setelah Diberi Cangkang Udang Laut (Penaeus monodon F.)

Bioma Berkala Ilmiah Biologi ◽

10.14710/bioma.17.2.118-122 ◽

2015 ◽

Vol 17 (2) ◽

pp. 118

Author(s):

Sri Isdadiyanto

Keyword(s):

Body Weight ◽

Penaeus Monodon ◽

Male Rats ◽

Sprague Dawley ◽

High Fat ◽

Group Iii ◽

Group I ◽

Sprague Dawley Rats ◽

Significant Difference ◽

The Difference

The objective of this study was to analyze the ratio of LDL/HDL level of Sprague Dawley rats induced by high fat ration after given of sea shrimp carapace (Penaeus monodon F.). The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes ratio of LDL/HDL level. LDL level and HDL level were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate. The difference between treatments was statistical analysis by Anava, and continued by Lowest Significant Difference Test to locate the difference. The result showed that high fat diet increased ratio of LDL/HDL level and Sea shrimp carapace decreased ratio of LDL/HDL level. Keywords: Sea shrimp carapace, Sprague Dawley rats, ratio of LDL/HDL level

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EFFECT OF VITAMIN E (α TOCOPHEROL) ADMINISTRATION ON APOPTOSIS OF GERMINAL CELLS EPITHELIUM TESTIS IN SPRAGUE DAWLEY WHITE STRAIN RATS AFTER EXPOSED BY CISPLATIN

Indonesian Journal of Urology ◽

10.32421/juri.v26i1.546 ◽

2019 ◽

Vol 26 (1) ◽

Author(s):

Achmad Nugroho ◽

Wahjoe Djatisoesanto ◽

Doddy M Soebadi

Keyword(s):

Vitamin E ◽

Epithelial Cells ◽

Protective Effect ◽

Positive Control ◽

Negative Control ◽

Sprague Dawley ◽

Group 4 ◽

Sprague Dawley Rats ◽

Significant Difference ◽

Group 2

Objective: To determine the differences of germinal epithelial testicular cell apoptosis in white Sprague Dawley strain rat that received combination of cisplatin and vitamin E compared to Sprague Dawley strain rat that received cisplatin only. Material & Methods: Twenty four Sprague Dawley rats were divided into 4 groups randomly. Group 1 Negative Control (NC) was given an injection of 1 cc 0.9% normal saline intraperitoneally as a placebo, group 2 Positive Control (PC) was given 5 mg/kgBW cisplatin intraperitoneally, group 3 (P1) was given cisplatin injection 5 mg/kgBW intraperitoneally + vitamin E (α tocopherol) 50 mg/kgBW by gavage and group 4 (P2) was given cisplatin injection 5 mg/kgBW intraperitoneally + vitamin E (α tocopherol) 200 mg/kgBW by gavage. Vitamin E (α tocopherol) was given 3 weeks before up to 4 weeks after cisplatin injection. Observation of the germinal epithelial cells apoptosis was carried out by calculating germinal epithelial cells apoptosis in the cross-section preparations of the seminiferous tubule which gave a positive reaction to the apoptag staining, using a 400x magnification light microscope. Results: Apoptosis on positive control (PC) group was different significantly compared to the negative control (NC) group (p<0.05). There was a significant difference in the apoptosis of germinal epithelial testicular cells in the cisplatin + vitamin E 50 mg/kgBW compared to the PC group (p<0.05). The cisplatin + vitamin E 200 mg/kgBW group; had a lower number of apoptosis compared to the cisplatin + vitamin E 50 mg/kgBW (p<0.05). Conclusion: Vitamin E provides a protective effect on decreasing the amount of apoptosis due to cisplatin exposure. The protective effect of vitamin E is dose-dependent.

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Curcumin Minimises Histopathological and Immunological Progression in the Ankle Joints of Collagen-Induced Arthritis Rats

Medicine & Health ◽

10.17576/mh.2020.1502.05 ◽

2020 ◽

Vol 15 (2) ◽

pp. 26-36

Author(s):

Taty Anna Kamarudin. ◽

Keyword(s):

Olive Oil ◽

Inflammatory Diseases ◽

Negative Control ◽

Sprague Dawley ◽

Collagen Induced Arthritis ◽

Sprague Dawley Rats ◽

Significant Difference ◽

Ankle Joints ◽

The Mean ◽

Random Groups

Curcumin is a traditionally used spice with a potential to treat various inflammatory diseases including arthritis. This study was aimed at observing curcumin’s effects on the histopathological progression and interleukin-1β (IL-1β) levels in collageninduced arthritis (CIA). Thirty male Sprague-Dawley rats (150+50 g) were divided into five random groups. A group was assigned as the normal control (CTRL), while the remaining were subcutaneously immunised with 150 μg of collagen emulsion on day 0. CTRL and CIA-Curcumin-d0 groups were supplemented daily with olive oil (1 ml/kg) and curcumin (110 mg/ml/kg) from day 0, respectively. The CIA-OV (negative control), CIA-Beta and CIA-Curcumin-d14 groups were given daily supplementation of olive oil (1 ml/kg), Betamethasone (0.5 mg/ml/kg), and curcumin (110 mg/ml/kg) from day 14, respectively. The daily oral supplementations continued until day 42. The study showed that CIA-Beta (**P<0.05) and CIACurcumin- d0 (**P=0.01) groups had significantly lower mean histological scores compared to CIA-OV, respectively. Serum IL-1β levels for CIA-Beta and CIACurcumin- d0 were not significantly raised on day 42 as to compared to day 0, and the mean increment of IL-1β levels from day 0 to day 42 were significantly lower (**P≤0.01) for all the CIA groups compared to CIA-OV. There was no significant difference in both mean histological scores and IL-1β levels of CIA-Curcumin-d0 compared to CIA-Beta. Early supplementation of curcumin could potentially minimise disease progression of CIA in rats.

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EFFECTIVENESS OF OZONATED VIRGIN COCONUT OIL ON FULL THICKNESS SKIN DEFECT WOUND HEALING IN SPRAGUE DAWLEY Rats

Medica Hospitalia : Journal of Clinical Medicine ◽

10.36408/mhjcm.v8i2.484 ◽

2021 ◽

Vol 8 (2) ◽

pp. 220-228

Author(s):

Herry Maha Putra Surbakti ◽

Renny Yuniati ◽

Djoko Handojo

Keyword(s):

Coconut Oil ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Full Thickness ◽

Tgf Beta ◽

Sprague Dawley ◽

Virgin Coconut Oil ◽

Sprague Dawley Rats ◽

Significant Difference

AbstractIntroduction: Wounds are a breakdown of tissue integrity / continuity that can lead to infection. Virgin Coconut Oil is a processed coconut product made by processing fresh coconut flesh at low temperature and has a high nutritional content. Ozone therapy is an alternative therapy that has disinfectant properties and can induce strong oxidative stress. Methods: This study was conducted on 50 Sprague-Dawley rats that had injuries. We performed full thickness wounds and administered ozonated doses of VCO to mice. We looked at shrinkage of wounds and TGF Beta levels in mice. Assessment was carried out on day 7 and day 14 to assess the wound and TGF-beta immunohistochemically. Results: There was a significant difference in the wound shrinkage variable between the negative control group and the group that received VCO offerings both on day 7 and day 14. We also found a significant difference in the TGF-beta variable between the negative control group and the group that received VCO offerings both on day 7 and day 14. Conclusion: The administration of ozonated virgin coconut oil was effective in increasing the expression of TGF-? in the full thickness defect of Sprague Dawley rats and was effective in increasing the reduction in wound size in the full thickness defect of Sprague Dawley rats.

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Adrenalectomy eliminates both fiber-type differences and starvation effects on denervated muscle

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1988.255.6.e850 ◽

1988 ◽

Vol 255 (6) ◽

pp. E850-E856 ◽

Cited By ~ 1

Author(s):

R. R. Almon ◽

D. C. Dubois

Keyword(s):

Muscle Mass ◽

Extensor Digitorum Longus ◽

Fiber Type ◽

Sprague Dawley ◽

Denervated Muscle ◽

Adrenalectomized Animal ◽

Sprague Dawley Rats ◽

Starvation Effects ◽

The Difference ◽

Denervated Muscles

This report describes changes in muscle mass of innervated and denervated pairs of muscles taken from intact and adrenalectomized 250-g male Sprague-Dawley rats provided with different diets. Diets ranged from a nutritionally complete liquid diet to starvation (water only). In the intact animals, muscles with a more tonic character (soleus) are less sensitive to starvation than are muscles with a more phasic character (extensor digitorum longus), whereas the opposite is true of denervation. In the intact animals, starvation greatly increased the amount of atrophy following denervation. In the adrenalectomized animals, starvation had no effect on the amounts of atrophy following denervation. Furthermore, adrenalectomy virtually eliminated the fiber-type differences in the amount of atrophy following denervation. In addition, a comparison between denervated muscles from intact animals and adrenalectomized animals subjected to starvation demonstrates that all denervated muscles from the adrenalectomized animals atrophy less. Finally, it was observed that although an adrenalectomized animal can tolerate 6 days of starvation, an adrenalectomized-castrated animal cannot tolerate even short periods of starvation. The difference appears to be due to low amounts of corticosterone of testicular origin.

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THE EFFECTS OF LEVELS OF ISOLATION, OR VARIETAL DIFFERENCES IN, HIGH FIBRE HULL FRACTION OF LOW GLUCOSINOLATE RAPESEED MEALS ON RAT OR PIG PERFORMANCE

Canadian Journal of Animal Science ◽

10.4141/cjas78-093 ◽

1978 ◽

Vol 58 (4) ◽

pp. 743-752 ◽

Cited By ~ 14

Author(s):

J. J. KENNELLY ◽

F. X. AHERNE ◽

A. J. LEWIS

Keyword(s):

Feed Intake ◽

Average Daily Gain ◽

Rapeseed Meal ◽

Sprague Dawley ◽

Crude Fibre ◽

Gain Ratio ◽

Sprague Dawley Rats ◽

Significant Difference ◽

Pig Performance ◽

Daily Gain

Forty-eight crossbred pigs of average initial weight 21 kg were fed 10% Tower rapeseed meal (RSM) and 10% Candle RSM as partial replacements for soybean meal (SBM). Diets were formulated to be isocaloric. Pigs fed the SBM diet consumed less feed, gained significantly (P < 0.01) faster and were more efficient at converting feed to gain than those fed the RSM diets. Performance of pigs fed Candle RSM was not significantly different to that obtained with Tower RSM. In a second experiment, dehulled Tower RSM and Tower RSM hulls were mixed in amounts to produce RSM with crude fibre levels of 6.8, 10.8, 13.5 and 15.8%. The simulated RSM and Tower and Candle RSM were used to completely replace SBM in the diets of weanling (75 g) Sprague-Dawley rats. Rats fed SBM had significantly (P < 0.05) higher average daily gain (ADG) than those fed Tower or Candle RSM, or diets containing the rapeseed meats. There was no significant (P < 0.05) difference in ADG, feed intake or feed to gain ratio of rats fed either Tower or Candle RSM. Feed intake, feed to gain ratio and fecal volatile fatty acid concentrations increased while average daily gain decreased with increasing level of hulls in simulated RSM diets. There was no significant difference (P < 0.05) in thyroid weight between rats fed SBM, Tower RSM or Candle RSM.

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Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

Human & Experimental Toxicology ◽

10.1177/0960327109353777 ◽

2009 ◽

Vol 29 (2) ◽

pp. 93-101 ◽

Cited By ~ 8

Author(s):

Amal A El-Bakary ◽

Sahar A El-Dakrory ◽

Sohayla M Attalla ◽

Nawal A Hasanein ◽

Hala A Malek

Keyword(s):

Alcohol Dehydrogenase ◽

High Performance ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Sprague Dawley ◽

Blood Samples ◽

Methanol Poisoning ◽

Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

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Oral administration of Lactobacillus casei Shirota can ameliorate the adverse effect of an acute aflatoxin exposure in Sprague Dawley rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000513 ◽

2018 ◽

Vol 88 (3-4) ◽

pp. 199-208

Author(s):

Elham Nikbakht ◽

Rosita Jamaluddin ◽

S. Mohd Redzwan ◽

Saman Khalesi

Keyword(s):

Adverse Effect ◽

Lactobacillus Casei ◽

Health Effect ◽

Acute Exposure ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Lactobacillus Casei Shirota ◽

Probiotic Treatment ◽

Significant Difference ◽

Aflatoxin B

Abstract. Aflatoxin B1 (AFB1) is a toxic compound commonly found in some crops with an adverse health effect on human and animals. Some beneficial microorganisms (or probiotics) such as lactic acid bacteria have shown the ability to reduce the bioavailability of aflatoxins and its intestinal absorption. However, the dose and duration of aflatoxins exposure and probiotic treatment can influence the ability of probiotics to remove aflatoxins. Therefore, this research aimed to investigate the efficacy of oral probiotic Lactobacillus casei Shirota strain (LcS) induction in an acute exposure to AFB1 in rats. Experimentally, Sprague Dawley rats were divided into three groups: AFB1 only (n = 9); AFB1 treated with LcS (n = 9); and control (no AFB1 exposure) (n = 6) groups. The blood AFB1 level of rats treated with LcS was slightly lower than the untreated AFB1 induced rats (11.12 ± 0.71 vs 10.93 ± 0.69 ng g–1). Also, LcS treatment slightly moderated the liver and kidney biomarkers in AFB1 induced rats. However, a trend for a significant difference was only observed in ALT of AFB1 induced rats treated with LcS compared to their counterparts (126.11 ± 36.90 vs 157.36 ± 15.46, p = 0.06). Rats’ body weight decreased in all animals force-fed with AFB1 with no significant difference between LcS treatment compared to the counterpart. In conclusion, this experiment indicated that probiotic LsC was able to slightly ameliorate the adverse effect of an acute exposure to AFB1 in rats. However, future studies with longer probiotics treatment or higher probiotics dose is required to confirm these findings.

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