Effect of Salinity, pH, Light Intensity on Growth and Lipid Production of Microalgae for Bioenergy Application

2015 ◽  
Vol 15 (4) ◽  
pp. 260-267 ◽  
Author(s):  
Monika Prakash Rai ◽  
Trishnamoni Gautom ◽  
Nikunj Sharma
Keyword(s):  
Light Intensity ◽  
Lipid Production

scholarly journals Statistical Approach of Nutrient Optimization for Microalgae Cultivation

2020 ◽  
Vol 141 ◽  
pp. 03009
Author(s):  
Pichayatorn Bunkaew ◽  
Sasithorn Kongruang
Keyword(s):  
Light Intensity ◽  
Cobalt Chloride ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Microalgae Cultivation ◽  
Cultivation Time ◽  
Chlorella Sp ◽  
Chloride Hexahydrate ◽  
Cobalt Chloride Hexahydrate

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

scholarly journals KERAGAMAN DAN KARAKTERISASI MIKROALGA DARI SUMBER AIR PANAS CIWALINI YANG BERPOTENSI SEBAGAI SUMBER BIODISEL

BIOSCIENTIAE ◽  
2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Gunawan Gunawan
Keyword(s):  
Growth Rate ◽  
Light Intensity ◽  
Lipid Content ◽  
Diversity Index ◽  
Nitrogen Concentration ◽  
Lipid Production ◽  
Nile Red ◽  
Continuous Light ◽  
Maximum Growth ◽  
Culture Technique

The objectives of the research were to obtain specific microalgae that are able to produce high lipid, and to determine suitable culture technique for optimum growth and maximum lipid production. Microalgae were identified, isolated, selected and then grown on IMK medium at 27-29oC under continuous light irradiation for 24 hours.   The microalgae were then selected for lipid content using Nile Red. The selected microalgae were then grown under the same medium and condition as before followed by selection based on their growth rate. To find an appropriate medium for specific microalgae, the selected microalgae were then grown on various media such as BG11, Zarrouk, MBM, PHM and BBM media.  When a medium was selected, it was then used as the medium for the nitrogen source and light intensity experiments. Those selected microalgae from each location were cultured on the selected medium at different nitrogen concentration (0,5, 1 and 2 M) and different light intensities (35, 70 and 140 µmol photon/m2/sec).  The result showed that Ciwalini has diversity index (H) 2,21. Identification indicated that ciwalini species was Chlorophyta. In this research maximum growth rate was at 2 M  nitrogen consentratiom with 140 µmol photon/m2/sec light intensity. Lipid content ranged from 11,7% - 28%. The highest lipid content was occurred on media 0,5 M nitrogen consentration and 140 µmol photon/m2/sec light intensity. Lipid Productivity ranged from 0,070,18 g/l/day.

scholarly journals Simultaneous nutrition removal and high-efficiency biomass and lipid accumulation by microalgae using anaerobic digested effluent from cattle manure combined with municipal wastewater

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Lin Luo ◽  
Hongyu Ren ◽  
Xuanyuan Pei ◽  
Guojun Xie ◽  
Defeng Xing ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Anaerobic Digestion ◽  
Light Intensity ◽  
Municipal Wastewater ◽  
High Efficiency ◽  
Lipid Production ◽  
Algal Growth ◽  
Economic Feasibility ◽  
Cattle Manure ◽  
Initial Ph

Abstract Background Microalgae as a viable biodiesel feedstock show great potential to approach the challenges of energy shortage and environment pollution, but their economic feasibility was seriously hampered by high production cost. Thus, it is in urgent need to reduce the cost of cultivation and improve the biomass and lipid production of microalgae. In this work, anaerobic digestion effluent from cattle manure combined with municipal wastewater was used as a cost-effective medium for cultivating microalgae and expected to obtain high biomass. The pretreatment of anaerobic digested effluent containing dilution rate, sterilization and nutrient optimization was investigated. Then, initial pH and light intensity for algal growth, lipid production and wastewater purification were optimized in this study. Results Scenedesmus sp. could grow rapidly in 10% anaerobic digestion effluent from cattle manure combined with secondary sedimentation tank effluent without sterilization. Optimum nutrient additives for higher biomass were as follows: glucose 10 g/L, NaNO3 0.3 g/L, K2HPO4·3H2O 0.01 g/L, MgSO4·7H2O 0.075 g/L and trace element A5 solution 1 mL/L. Biomass of 4.65 g/L and lipid productivity of 81.90 mg/L/day were achieved during 7-day cultivation accompanying over 90% of COD, NO3−-N, NH4+-N, and 79–88% of PO43−-P removal with optimized initial pH of 7.0 and light intensity of 5000 l×. The FAME profile in ADEC growth medium consisted in saturated (39.48%) and monounsaturated (60.52%) fatty acids with the 16- to 18-chain-length fatty acids constituting over 98% of total FAME. Conclusions This study proves the potential of anaerobic digested effluent combined with municipal wastewater for microalgae culture, and provides an effective avenue for simultaneous microalgal lipid production and treatment of two kinds of wastewater.

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