scholarly journals The Influence of Trichoderma viride Cellulase Enzyme Concentration on Saccharification of Waste Paper Materials

2021 ◽  
Vol 21 (4) ◽  
pp. 279-287
Author(s):  
Khomotso M. P. Mokatse ◽  
M. Alfred Mogale ◽  
J. Pieter H. Van Wyk
Keyword(s):  
Trichoderma Viride ◽  
Enzyme Concentration ◽  
Waste Paper ◽  
Cellulase Enzyme

Optimization of chemical and enzymatic deinking of photocopier waste paper

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 447-463 ◽  
Author(s):  
Puneet Pathak ◽  
Nishi K. Bhardwaj ◽  
Ajay K. Singh
Keyword(s):  
Physical Properties ◽  
Removal Efficiency ◽  
Strength Properties ◽  
Waste Paper ◽  
Cellulase Enzyme ◽  
Tensile Index ◽  
Drainage Time ◽  
Tear Index ◽  
The World ◽  
Better Than

The utilization of post-consumer papers in the production of new paper products is increasing all over the world in recent years. Recycling of photocopier paper is a major problem due to difficulty in removal of non-impact ink. Enzymes offer potential advantages in ecofriendly deinking of recovered paper. In this study the deinking of photocopier paper was examined using chemicals and a commercial cellulase enzyme. Parameters of deinking experiments were optimized for hydrapulping. The ink was removed by flotation and washing processes. Then these parameters were compared in terms of ink removal ability of the process, as well as optical and strength properties of the deinked paper. The application of enzymatic deinking improved ink removal efficiency by 24.6% and freeness by 21.6% with a reduction in drainage time of 11.5% in comparison to those obtained with chemical deinking. The physical properties, namely burst index and tensile index, were observed to improve by 15.3% and 2.7%, respectively and brightness and tear index decreased by 2.1% and 21.9%, respectively. Results of deinking efficiency of photocopier paper showed that the enzyme used in the present work performed better than the conventional chemicals used for deinking.

scholarly journals Successive Saccharification of Waste Paper as a Resource for Bio-product Development

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
K.M.P. Mokatse ◽  
J.P.H. van Wyk
Keyword(s):  
Environmental Pollution ◽  
Incubation Period ◽  
Filter Paper ◽  
Fossil Fuels ◽  
Trichoderma Viride ◽  
Value Added ◽  
Sugar Concentration ◽  
Waste Paper ◽  
Waste Biomass ◽  
Incubation Periods

Environmental pollution and the exploitation of fossil-based products are topical issues that should be a matter of concern to the global population. The production of bio-based substances from waste biomass is a way to reduce the consumption of fossil fuels and limit environmental pollution. Enzymatic catalysed saccharification of cellulose is an important step for the bio-conversion of biomass such as waste paper into glucose that could be utilized as a feedstock for the production of value added bioproducts and this process can also be considered as an alternative route of waste management. During this study, fresh cellulase enzyme from Trichoderma viride was incubated separately with seven different waste paper materials during twelve successive incubation periods of 2 h each. The amount of sugar released from each paper material during each incubation period was determined. The highest sugar concentration released from each paper materials was produced during the first incubation period except the filter paper for which the highest amount of sugar was produced during the 9th period of incubation. During these optimum sugar producing incubation periods the highest total sugar concentration was released from brown envelope paper (3.3 mg.mL-1 followed by foolscap paper (3.0 mg.mL-1) and office paper (2.8 mg.mL-1) while the lowest amount of sugar was released from Pick ’n Pay paper (0.6 mg.mL-1). The relative saccharification percentage was also calculated which showed that filter paper produced the highest amounts of sugar followed by newspaper, and foolscap paper with advertising paper from a retailer. Pick ’n Pay offered the highest resistance towards cellulase catalysed bio-conversion into sugar.

scholarly journals RESEARCH ON CELLULASE AND PECTINASE ENZYME FROM TRICHODERMA VIRIDE AND ASPERGILLUS NIGER IN ORDER TO PROCESS COFFEE PULP QUICKLY

2009 ◽  
Vol 12 (13) ◽  
pp. 50-56
Author(s):  
Thuan Thi Thanh Tran ◽  
Luong Duc Nguyen
Keyword(s):  
Aspergillus Niger ◽  
Production Line ◽  
Trichoderma Viride ◽  
Waste Material ◽  
Optimum Conditions ◽  
Coffee Production ◽  
Coffee Pulp ◽  
Cellulase Enzyme ◽  
Pectinase Enzyme

Coffee pulp, which is waste material from coffee production line, has many poor natural degradeble property. Cellulase aillus nigernd pectinase, which were bio synthesized by the fugus races of Trichoderma viride and Asperg, can improve the disintergration process of coffee pulp. In this study, the conditions affecting the production of these enzymes were studied.. The optimum conditions for producing Pectinase enzyme of Aspergillus niger include: 2 days, 62% of humidity, 8% of culture. The optimum conditions for the production of Cellulase enzyme of Trichoderma viride are lasting for 4 days, 58% of humidity, 8% of culture. By carrying out real experiments we found that coffee pulp was disintergrated in the duration of 14 days, 60% of humidity, 8% of culture.

scholarly journals Optimization of Cellulase Enzyme Production by Co-cultures of Fungi Isolated from Lignocellulosic Waste

2020 ◽  
Vol 6 (5) ◽  
pp. 19-26
Author(s):  
Francis John V ◽  
Dr. Soloman P A
Keyword(s):  
Moisture Content ◽  
Incubation Time ◽  
Substrate Concentration ◽  
Enzyme Production ◽  
Trichoderma Viride ◽  
Cellulase Production ◽  
Optimal Conditions ◽  
Lignocellulosic Waste ◽  
Cellulase Enzyme ◽  
Degree Of Degradation

Fruit wastes were incubated with the mixture of cellulolytic fungi Penicillium citrinum, Aspergillus oryzae, and Trichoderma viride to hydrolyze the cellulosic components and to increase the degree of degradation. . The batch experiments are statistically designed and performed using Box-Benhken method of Response Surface Methodology to investigate the influence of major parameters viz., incubation time, temperature, pH, moisture content and substrate concentration on cellulase enzyme production. Maximum cellulase production of 2.03 Units/ml (U/ml) was detected by the RSM method in a mixed culture containing fungi at a ratio of 1: 1: 1 under optimal conditions at an incubation time of 5.27 days, a temperature of 34.09 °C, pH 4.85, moisture content of 63.83% and a substrate concentration of 5.03%.

Optimization of enzymatic hydrolysis for ethanol production by simultaneous saccharification and fermentation of wastepaper

2011 ◽  
Vol 29 (11) ◽  
pp. 1134-1144 ◽  
Author(s):  
Kanokphorn Sangkharak
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Incubation Time ◽  
Simultaneous Saccharification And Fermentation ◽  
Trichoderma Viride ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Sugar Production ◽  
Simultaneous Saccharification ◽  
Office Paper

The present study investigated the development of high sugar production by optimization of an enzymatic hydrolysis process using both conventional and statistical methods, as well as the production of ethanol by the selected wastepaper source. Among four sources of pretreated wastepaper including office paper, newspaper, handbills and cardboard, office paper gave the highest values of cellulose (87.12%) and holocelluloses (89.07%). The effects of the amount of wastepaper, the pretreatment method and the type of enzyme on reducing sugar production from office paper were studied using conventional methods. The highest reducing sugar production (1851.28 µg L−1; 37.03% conversion of glucose) was obtained from the optimal condition containing 40 mg of office paper, pretreated with stream explosion and hydrolysed with the combination of cellulase from Aspergillus niger and Trichoderma viride at the fixed loading rate of 20 FPU g−1 sample. The effects of interaction of wastepaper amount and enzyme concentration as well as incubation time were studied by a statistical method using central composite design. The optimal medium composition consisted of 43.97 µg L−1, 28.14 FPU g−1 sample and 53.73 h of wastepaper, enzyme concentration and incubation time, respectively, and gave the highest amount of sugar production (2184.22 µg L−1) and percentage conversion of glucose (43.68%). The ethanol production from pretreated office paper using Saccharomyces cerevisiae in a simultaneous saccharification and fermentation process was 21.02 g L−1 after 36 h of cultivation, corresponding to an ethanol volumetric production rate of 0.58 g ethanol L−1 h−1.

scholarly journals Antagonistic activity of cellulase enzyme produced by Trichoderma Viride against Xanthomonas Citr

2018 ◽  
Author(s):  
Prabhat Jatav ◽  
Sonu Singh Ahirwar ◽  
Abhishek Gupta ◽  
Kirti Kushwaha ◽  
Sadhna Jatav
Keyword(s):  
Antibacterial Activity ◽  
Soil Fungi ◽  
Trichoderma Viride ◽  
Citrus Canker ◽  
Antibacterial Activities ◽  
Control Agent ◽  
Antagonistic Activity ◽  
Bacterial Disease ◽  
Cellulase Enzyme ◽  
Citrus Disease

Citrus canker is a bacterial disease which occurs in citrus (lemon) species produce by Xanthomonas especially X. citri. The infection occurs on the lesions of leaves, stems, and fruits of citrus trees, it also occurs in other plants such as orange, grapes. The main goal of this study was to prevent the lemon (citrus) disease such as citrus canker by using some cellulase enzymes produces by soil fungi. In the present study, we isolated soil fungi Trichoderma viride, which produces cellulase enzyme these have higher antibacterial activities against plant pathogen such as X. citri. This enzyme produced by T. viride by using fermentation method, quantified by DNAS method and used as a bio-control agent against X. citri. They also isolated and characterized X. citri. In the last step of the study they have checked antibacterial activity of cellulase enzyme against X. citri. The present study concluded that cellulase enzyme produced by T. viride shows higher antibacterial activity against X. citri when treated it at 100°C.

scholarly journals Isolation and Biochemical Characterization of Cellulase Produced by Bacterial Isolates from Sugarcane Waste Soil

2021 ◽  
pp. 28-35
Author(s):  
A. U. Hassan ◽  
R. Jafaru ◽  
I. B. Mato ◽  
E. Kereakede ◽  
A. H. Galadanci ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Biochemical Characterization ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Enzyme Concentration ◽  
Microbiological Analysis ◽  
Cellulase Enzyme ◽  
Sds Page ◽  
Important Enzyme

Cellulase is one of the most economically important enzyme, which aids in catalyzing cellulolysis, the decomposition of cellulose and other related polysaccharides. So the demand/importance of this enzyme in both domestic and commercial sectors cannot be over emphasized. In this research cellulase-producing bacteria were isolated from soil around sugarcane waste dumping area, which was identified to be P. fulorescens after numerous biochemical and microbiological analysis. The bacteria were then grown and used to ferment certain biomass, with the aim of using the organisms to produce the cellulase enzyme. The total protein/cellulase enzyme activity of the medium was ascertained. Optimization/characterization for maximum cellulase activity was done by varying the temperature, pH, enzyme concentration and substrate concentration, in which the optimum condition for cellulase production was ascertain to be at a temperature and pH of 40˚C and pH 7 respectively. SDS-PAGE electrophoresis was carried out to determine and reconfirm the presence and molecular weight of the isolated enzyme. The estimated extrapolated molecular weight of the enzyme was found to be 13.5KDa.

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