In Vivo Toxicity Study of Ethanolic Extracts of Evolvulus alsinoides & Centella asiatica in Swiss Albino Mice

AIM: We aimed to investigate several parameters after the in vivo acute and sub-acute administration of ethanolic extracts from E. alsinoides & C. asiatica. METHODS: Malignant Ovarian Germ Cell Tumors for in vivo toxicity study guidelines 423 and 407 of Organization for Economic Co-operation and Development (OECD) were followed for acute and sub-acute toxicity assays respectively. For LD50 evaluation, a single dose of ethanolic extracts of Evolvulus alsinoides L. (EEA) and ethanolic extracts of Centella asiatica (ECA) was orally administered to mice at doses of 200, 400, 800, 1600 and 2000 mg/kg. Then the animals were observed for 72 hours. For acute toxicity evaluation, a single dose of both extracts was orally administered to mice at doses of 300, 600, 1200 and 2000 mg/kg and the animals were observed for 14 days. In the sub-acute study, the extracts were orally administered to mice for 28 days at doses of 300, 600, 1200 and 2000 mg/kg. To assess the toxicological effects, animals were closely observed on general behaviour, clinical signs of toxicity, body weight, food and water intake. At the end of the study, it was performed biochemical and hematological evaluations, as well as histopathological analysis from the following organs: brain, heart, liver, and kidney. RESULTS: The oral administration of E. alsinoides and C. asiatica ethanolic extracts, i.e. EEA 300, EEA 600, EEA 1200, EEA 2000, ECA 300, ECA 600, ECA 1200 & ECA 2000 mg/kg doses showed no moral toxicity effect in LD50, acute and sub-acute toxicity parameters. CONCLUSION: In this study, we had found that E. alsinoides & C. asiatica extract at different doses cause no mortality in acute and sub-acute toxicity study. Also, histopathology of kidney, liver, heart, and brain showed no alterations in tissues morphology.

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Neuroprotective Activity of Evolvulus alsinoides & Centella asiatica Ethanolic Extracts in Scopolamine-Induced Amnesia in Swiss Albino Mice

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.247 ◽

2019 ◽

Vol 7 (7) ◽

pp. 1059-1066 ◽

Cited By ~ 4

Author(s):

Mukesh Kumar Yadav ◽

Santosh Kumar Singh ◽

Manish Singh ◽

Shashank Shekhar Mishra ◽

Anurag Kumar Singh ◽

...

Keyword(s):

Neuroprotective Effect ◽

Centella Asiatica ◽

Neuroprotective Activity ◽

Immunomodulatory Activity ◽

Histopathological Analysis ◽

Nootropic Activity ◽

Swiss Albino Mice ◽

Evolvulus Alsinoides ◽

Ethanolic Extracts ◽

Albino Mice

AIM: To carry out the comparative nootropic, neuroprotective potentials of two medicinal plant species. MATERIAL AND METHODS: For neuroprotective activity; behavior models (elevated plus maze & morris water maze), in vivo antioxidant (superoxide dismutase, catalase, lipid peroxidation & reduced glutathione), inflammatory markers (IL-1β, IL-6 & TNF-α) and acetylcholine esterase (AChE) assessment procedures followed at different dosages i.e. 250 & 500 mg/kg of Evolvulus alsinoides and Centella asiatica ethanolic extracts. At the end of the study, it was performed histopathological analysis of the following organs: brain, heart, liver, and kidney. RESULTS: In oral administration of different doses of ethanolic extracts of both medicinal plants i.e. Sco + EEA 250 = 2.49 ± 0.29 , Sco + EEA 500 = 2.67 ± 0.36, Sco + ECA 250 = 2.33 ± 0.17, Sco + ECA 500 = 2.77 ± 0.21, Sco + EEA + ECA 250 = 2.61 ± 0.32 and Sco + EEA + ECA 500 = 2.79 ± 0.16 U/mg of protein respectively against the scopolamine induced group Sco (control) = 5.51 ± 0.35 U/mg of protein extracts shows neuroprotective and nootropic activity with reducing AChE level in the brain homogenate of swiss albino mice. CONCLUSION: Since the E. alsinoides & C. asiatica are already used in traditional Indian medicine as the neuroprotective agent and also found promising effects over inflammatory diseases, wound healing, and immunomodulatory activity. The neuroprotective effect of both plants extracts attributed to inhibition of AChE activity and improve the spatial memory formation.

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In-vitro and in-vivo evaluation of antidiabetic activity of Withania coagulans extract

The Natural Products Journal ◽

10.2174/2210315509666190416155757 ◽

2019 ◽

Vol 09 ◽

Author(s):

Tejas Patel ◽

B.N. Suhagia

Keyword(s):

Blood Glucose ◽

Acute Toxicity ◽

Aqueous Extract ◽

Pancreatic Beta Cell ◽

Toxicity Study ◽

Acute Toxicity Study ◽

Withania Coagulans ◽

Study Results

Background: Diabetes mellitus is major issue to public health as its prevalence is rising day by day. Synthetic agents available for the diabetic treatment are expensive or produce undesirable side effect on chronic use and some of them are not suitable during pregnancy. Herbal medicines accepted widely due to side effects and low cost. Objective: The aim of present study was to evaluate the activity of Withania coagulans extract using In-vitro and In-vivo model. Methods: Different three types of Withania coagulans extract were prepared using aqueous (W1), Alcohol (W2) and hydro-alcoholic (50:50) mixture (W3). In-vitro Anti-diabetic activity of the all three extracts evaluated using RINm5F Pancreatic beta cells.Further, n-vivo anti-diabetic evaluation performed by administering 50 mg/kg (p.o) aqueous extract for 7 days in Streptozotocin (STZ)-induced mice. Body weight of the animals was also determined to perform acute toxicity study. Results: The results of in –vitro cell based study indicated that among all three extract, aqueous extract (W1) of Withania coagulans showed potential increase in inulin release. The EC50 of the W1 (249.6 µg/L) which is compared with standard (Glibenclamide) EC50. From the results of In-vitro study, W1 subjected for acute toxicity study and the acute toxicity study results indicated LD50 of 50mg/kg. Diabetic rats treated with W1 extract at oral dose of 50 mg/kg for 7 days showed 34.17% reduction in blood glucose in comparison to untreated diabetic (STZ-induced) rats. Blood glucose levels of Standard treated (Glibenclamide) and control untreated. Conclusion: In conclusion, results of pancreatic beta cell based study showed increase in insulin release by administration of extract. Further aqueous extract (W1) was potentially reduced blood glucose level in STZ induced diabetic mice.

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In vitro characterization and in vivo toxicity study of repaglinide loaded poly (methyl methacrylate) nanoparticles

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2010.06.023 ◽

2010 ◽

Vol 396 (1-2) ◽

pp. 194-203 ◽

Cited By ~ 60

Author(s):

U.M. Dhana lekshmi ◽

G. Poovi ◽

Narra Kishore ◽

P. Neelakanta Reddy

Keyword(s):

Methyl Methacrylate ◽

Toxicity Study ◽

In Vivo Toxicity ◽

Poly Methyl Methacrylate ◽

In Vitro Characterization

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Development and Characterization of siRNA Lipoplexes: Effect of Different Lipids, In Vitro Evaluation in Cancerous Cell Lines and In Vivo Toxicity Study

AAPS PharmSciTech ◽

10.1208/s12249-014-0193-9 ◽

2014 ◽

Vol 15 (6) ◽

pp. 1630-1643 ◽

Cited By ~ 23

Author(s):

Nirav Khatri ◽

Dipesh Baradia ◽

Imran Vhora ◽

Mohan Rathi ◽

Ambikanandan Misra

Keyword(s):

Cell Lines ◽

Toxicity Study ◽

In Vitro Evaluation ◽

In Vivo Toxicity ◽

Cancerous Cell

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Acute and subacute toxicity profiles on siddha drug Thulasi Ennai in wistar rats

The Journal of Phytopharmacology ◽

10.31254/phyto.2020.9604 ◽

2020 ◽

Vol 9 (6) ◽

pp. 403-409

Author(s):

S Sonitha ◽

◽

D Sivaraman ◽

V Rani ◽

◽

...

Keyword(s):

Body Weight ◽

Medicinal Plants ◽

Clinical Signs ◽

Toxicity Study ◽

Albino Rats ◽

Physical Parameters ◽

Histopathological Analysis ◽

Common Chronic Disease ◽

Subacute Toxicity

Medicinal plants have been used in traditional medicines for their unmatched availability of bioactive compounds. Asthma is the most common chronic disease among children worldwide. It is ranked 16th among the leading causes of years lived with disability. Medicinal plants have placed a vital role in the siddha system of medicine over centuries to cure acute and chronic illness. The aim of the present study was to investigate toxicity analysis to evaluate safety of the siddha drug Thulasi Ennai in vivo in wistar albino rats. Thulasi Ennai is a polyherbal siddha formulation mentioned in the ancient siddha books and literature, indicated to cure childhood bronchial asthma. In this study, Thulasi Ennai administered orally at a single dose of 2000mg/kg body weight and monitored for 14 days. For subacute toxicity study, Thulasi Ennai was orally administered in different doses of 200,400mg/kg body weight, daily for 28 days. At the end of each study physical parameters, hematological, biochemical and histopathological analysis were evaluated. No animals in each group of acute or subacute toxicity study showed mortality or clinical signs of toxicity throughout the study. Hence, the results of the study indicate a safe toxicological profile of Thulasi Ennai.

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Acute and sub-chronic oral toxicities of DA.AMLODEPON HVD hard capsule in experimental animals

Tạp chí Nghiên cứu Y học ◽

10.52852/tcncyh.v148i12.432 ◽

2021 ◽

Vol 148 (12) ◽

pp. 58-67

Author(s):

Pham Thi Van Anh ◽

Nguyen Van Dam ◽

Nguyen Van Dat ◽

Pham Thanh Ky ◽

Nguyen Trong Thong ◽

...

Keyword(s):

Body Weight ◽

Acute Toxicity ◽

Chronic Toxicity ◽

The Body ◽

Toxicity Study ◽

World Health ◽

Histopathological Analysis ◽

Experimental Animals ◽

Health Organization ◽

General Conditions

Assessment of toxicities of DA.AMLODEPON HVD hard capsule on experimental animals. The acute toxicity of DA.AMLODEPON HVD was assessed on Swiss mice according to World Health Organization Guidance, and LD50 determination according to the method of Litchfield – Wilcoxon. The sub-chronic toxicity study of DA.AMLODEPON HVD at two doses (0.42 g/kg/day and 1.26g/kg/day) was conducted in rats for four consecutive weeks. After administration, general conditions and the body weight of rats were evaluated. Blood samples were collected for analyzing serum parameters before treatment (T0), second week (T1), and fourth week (T2). Histopathological analysis of livers and kidneys was observed at the end of the experiment. The results revealed that mice were taken up to a maximum dose of 39.15 g/kg with no symptoms of acute toxicity, LD50 of DA.AMLODEPON HVD has not been determined. The sub-chronic toxicity study at two doses did not change the body weight of rats, general conditions. The parameters for structures and functions of livers and kidneys and microscopic of the livers and kidneys are in a normal range during the study period.

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Preparation and in vivo toxicity study of solid lipid microparticles as carrier for pulmonary administration

AAPS PharmSciTech ◽

10.1208/pt050227 ◽

2004 ◽

Vol 5 (2) ◽

pp. 17-23 ◽

Cited By ~ 50

Author(s):

Vanna Sanna ◽

Nathalie Kirschvink ◽

Pascal Gustin ◽

Elisabeta Gavini ◽

Isabelle Roland ◽

...

Keyword(s):

Toxicity Study ◽

In Vivo Toxicity ◽

Pulmonary Administration ◽

Solid Lipid ◽

Solid Lipid Microparticles ◽

Lipid Microparticles

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Amphiphilic poly-N-vinylpyrrolidone nanoparticles as carriers for non-steroidal, anti-inflammatory drugs: In vitro cytotoxicity and in vivo acute toxicity study

Nanomedicine Nanotechnology Biology and Medicine ◽

10.1016/j.nano.2016.11.006 ◽

2017 ◽

Vol 13 (3) ◽

pp. 1021-1030 ◽

Cited By ~ 20

Author(s):

Andrey N. Kuskov ◽

Pavel P. Kulikov ◽

Anastasia V. Goryachaya ◽

Manolis N. Tzatzarakis ◽

Anca O. Docea ◽

...

Keyword(s):

Acute Toxicity ◽

In Vitro Cytotoxicity ◽

Toxicity Study ◽

Acute Toxicity Study ◽

Inflammatory Drugs ◽

Anti Inflammatory

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Toxicological studies of aqueous extract of Acacia nilotica root

Interdisciplinary Toxicology ◽

10.1515/intox-2015-0005 ◽

2015 ◽

Vol 8 (1) ◽

pp. 48-54 ◽

Cited By ~ 6

Author(s):

Lukman Adewale Alli ◽

Abdulfatai Ayoade Adesokan ◽

Oluwakanyinsola Adeola Salawu ◽

Musbau Adewunmi Akanji

Keyword(s):

Body Weight ◽

Single Dose ◽

Acute Toxicity ◽

Aqueous Extract ◽

Liver Homogenate ◽

Acacia Nilotica ◽

Acute Administration ◽

Weight Changes ◽

African Countries ◽

Toxicological Studies

AbstractAcacia niloticais a widely used plant in traditional medical practice in Northern Nigeria and many African countries. The aim of this study was to determine the toxicological effects of a single dose (acute) and of repeated doses (sub-acute) administration of aqueous extract ofA. niloticaroot in rodents, following our earlier study on antiplasmodial activity. In the acute toxicity test, three groups of Swiss albino mice were orally administered aqueous extract ofA. nilotica(50, 300 and 2000 mg/kg body weight) and signs of toxicity were observed daily for 14 days. In the sub-acute toxicity study, four groups of 12 rats (6 male and 6 female) were used. Group 1 received 10 ml/kg b.w distilled water (control), while groups 2, 3 and 4 received 125, 250 and 500 mg/kg b.w of the extract, respectively, for 28 consecutive days by oral gavage. Signs of toxicity/mortality, food and water intake and body weight changes were observed. Biochemical parameters were analysed in both plasma and liver homogenate. In the acute and sub-acute toxicity studies, the extract did not cause mortality. A significant reduction in the activity of lactate dehydrogenase was observed at 250 and 500 mg/kg b.w, while alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities were significantly higher than control values at 500 mg/kg b.w. The aqueous extract ofA. niloticawas found to be safe in single dose administration in mice but repeated administration of doses higher than 250 mg/kg b.w of the extract for 28 days in rats may cause hepatotoxicity.

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