scholarly journals The Effect of Giving Dadih on Manganese Superoxide Dismutase Gene Expression and Malondialdehyde Levels at Aging Kidney

2020 ◽  
Vol 8 (A) ◽  
pp. 525-528
Author(s):  
Harnavi Harun ◽  
Yanwirasti Yanwirasti ◽  
Bambang Purwanto ◽  
Endang Purwati Rahayuningsih
Keyword(s):  
Gene Expression ◽  
Superoxide Dismutase ◽  
Aging Process ◽  
Manganese Superoxide Dismutase ◽  
Kidney Tissue ◽  
Manganese Superoxide ◽  
Group 3 ◽  
Group 2 ◽  
Aging Kidney ◽  
Group 1

BACKGROUND: The aging process is a complex physiological mechanism that cannot be disclosed in exact detail. Various theories about the aging process have been put forward by many experts. AIM: This study aims to prove the effect of dadih on manganese superoxide dismutase (MnSOD) gene expression and malondialdehyde levels at aging kidney. MATERIALS AND METHODS: This study used 30 Rattus norvegicus which were divided into three groups: Group 1 was a positive control (did not get dadih), Group 2 received dadih 1 × 4.5 g/day, and Group 3 received dadih 2 × 4.5 g/day for 42 days. After that, MDA levels of kidney tissue are examined using the thiobarbituric acid reactive substances examination technique and examination of kidney and examination of MnSOD was done by immunohistochemical staining of kidney tissue. Data were analyzed using the normality test with Shapiro-Wilk. RESULTS: The results showed that dadih were be able to decreased MDA levels in Group 2 and Group 3 compared to Group 1 (control) 0.97 ± 0.06 pg/mL to 0.75 ± 0.03 (p < 0.05). Dadih also increased MnSOD expression sequentially from Group 1 33.66 ± 9.29 to Group 2 53.58 ± 8.51 and Group 3 73.70 ± 4.29 (p < 0.05) CONCLUSION: This study concluded that dadih can reduce MDA levels in old kidney tissue and dadih can increase the expression of MnSOD.

scholarly journals Antioxidant Effect of MnTE-2-PyP on Lung in Asthma Mice Model

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lyudmil Terziev ◽  
Violeta Dancheva ◽  
Veneta Shopova ◽  
Galya Stavreva
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Control Group ◽  
Mice Model ◽  
Antioxidant Defence ◽  
Group 4 ◽  
Antioxidant Defence System ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. To investigate the effects of MnTE-2-PyP on some markers of antioxidant defence system in asthma mice model.Material and Methods. The animals were divided into four groups: group 1, controls; group 2, injected with ovalbumin, group 3, treated with MnTE-2-PyP, and group 4, treated with ovalbumin and MnTE-2-PyP. The activities of superoxide dismutase, catalase, glutathione peroxidase and nonprotein sulfhydryl groups content (NPSH) were determined in lung homogenate.Results. The activities of superoxide dismutase and catalase in group 2 decreased significantly as compared to control group. The decrease of the same enzymes in group 4 was lower and significant as compared to group 2. Changes in the glutathione peroxidase activity showed a similar dynamics. The NPSH groups content decreased in group 2. In group 4 this decrease was relatively lower as compared to group 2.Conclusions. The application of MnTE-2-PyP mitigated the effects of oxidative stress in asthma mice model.

Investigation of TAp63 gene Expression and Follicle Count Using Melatonin in Cisplatin-induced Ovarian Toxicity

2020 ◽  
Author(s):  
Hacı Öztürk Şahin ◽  
Mehmet Nuri Duran ◽  
Fatma Sılan ◽  
Ece Sılan ◽  
Duygu Sıddıkoglu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Female Rats ◽  
Histological Evaluation ◽  
Saline Control ◽  
Control Group ◽  
Ovarian Toxicity ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract Background: Premature ovarian failure is among the most important side effects of chemotherapy during reproductive period. Preserving ovarian function is gradually gaining importance during oncologic treatment. The present study aims to investigate the potential of melatonin to protect from cisplatin-induced ovarian toxicity in rats. Twenty nine female rats were divided to three groups: Saline control group (Group 1), cisplatin group (Group 2), and cisplatin+melatonin group (Group 3). While the rats in Groups 2 and 3 were administered 5 mg/kg single dose of cisplatin via intra-peritoneal (IP) route, the rats in Group 3 were started on melatonin (20 mg/kg IP) before cisplatin administration and continued during 3 consecutive days. Ovaries were removed one week after cisplatin administration in all groups. Blood samples were obtained before the rats were decapited. Histological evaluation, follicle count, and classification were performed. TAp63 mRNA expression was evaluated using mRNA extraction and real-time polymerase chain reaction (PCR) method. Serum estradiol (E2) and anti-mullerian hormone (AMH) values were measured with enzyme immune-assay technology. Results: While primordial follicles were seen to decrease in Group 2 as compared to Group 1 (p:0.023), primordial follicle count was observed to be preserved significantly in melatonin group as compared to Group 2 (p:0.047). Moreover, cisplatin-induced histo-pathological morphology was preserved in favor of normal histology in melatonin group. A significant difference was not observed between groups with regard to mean serum AMH and E2 values (p:0.102 and p:0.411, respectively). While TAp63 gene expression significantly increased in Group 2 as compared to control group (p:0.001), we did not detect a statistically significant difference in cisplatin+melatonin group, although gene expression decreased (p:0.34). Conclusion: We conclude that concurrent administration of melatonin and cisplatin may protect from ovarian damage.

Rat lung antioxidant enzymes: differences in perinatal gene expression and regulation

1992 ◽  
Vol 263 (4) ◽  
pp. L466-L470 ◽  
Author(s):  
L. B. Clerch ◽  
D. Massaro
Keyword(s):  
Gene Expression ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Manganese Superoxide Dismutase ◽  
Late Gestation ◽  
Postnatal Exposure ◽  
Sod Activity ◽  
Copper Zinc Superoxide Dismutase ◽  
Manganese Superoxide ◽  
Copper Zinc

The lung activity of the antioxidant enzymes (AOEs) copper, zinc superoxide dismutase (Cu,Zn SOD), catalase (CAT), and glutathione peroxidase (GP), but not manganese superoxide dismutase (Mn SOD), increases in rats during late gestation; the concentrations of Cu,Zn SOD mRNA and CAT mRNA also rise. During early postnatal exposure to > 95% O2, the lung activity of Cu,Zn SOD, CAT, and GP increases. We now show 1) the lung concentration of Mn SOD mRNA and GP mRNA does not increase in late gestation; 2) Mn SOD activity and the concentration of its mRNA and of GP mRNA increase during exposure of neonatal rats to > 95% O2; and 3) as previously shown for CAT mRNA, the increase in lung concentration of the mRNAs for Cu,Zn SOD, Mn SOD, and GP during early postnatal hyperoxia occurs with a 70–80% prolongation of the half-life of these mRNAs. We conclude that 1) in late gestation the level at which lung AOE gene expression is regulated differs among the enzymes, 2) the level at which lung AOE gene expression is regulated shortly after birth in response to > 95% O2 is uniform among the enzymes, and 3) the lung's AOE response to neonatal hyperoxia is not merely a step-up of its prenatal regulation but involves different regulatory mechanisms based on increased stability of AOE mRNAs

Optimising prediction of early metastasis-free survival in uveal melanoma using a four-category model incorporating gene expression profile and tumour size

2021 ◽  
pp. bjophthalmol-2020-317714
Author(s):  
Kelsey Andrea Roelofs ◽  
Parampal Grewal ◽  
Steven Lapere ◽  
Matthew Larocque ◽  
Albert Murtha ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Information Criteria ◽  
Free Survival ◽  
Group 4 ◽  
Prognostic Groups ◽  
Class 1 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

BackgroundLargest basal diameter (LBD) appears to have independent prognostic value in uveal melanoma (UM).MethodsAll patients undergoing plaque brachytherapy or enucleation for UM involving the choroid and/or ciliary body between 2012 and 2019.ResultsA total of 348 patients with a mean age of 60±14 years were included and followed for a mean of 40±26 months (3.3±2.2 years). On multivariate analysis, LBD >12 mm remained a significant independent predictor of metastasis for both class 1 (HR 21.90; 95% CI 2.69 to 178.02; p=0.004) and class 2 (HR 2.45; 95% CI, 1.03 to 5.83; p=0.04) tumours. Four prognostic groups were created: group 1 (class 1, LBD <12 mm), group 2 (class 1, LBD ≥12 mm), group 3 (class 2, LBD <12 mm) and group 4 (class 2, LBD ≥12 mm). Life tables were used to calculate the 3-year and 5-year metastasis-free survival: group 1 (98 and 98%), group 2 (86 and 86%), group 3 (81 and 62%) and group 4 (54 and 47%). Compared with the reference category (group 1), the Cox proportional hazard model demonstrated a significant worsening of survival for each progressive category (group 2 (HR 21.59; p=0.004), group 3 (HR 47.12, p<0.001), and group 4 (HR 114.24; p<0.001)). In our dataset, the four-category Cox model performed poorer compared with the American Joint Committee on Cancer (AJCC) and gene expression profile (AJCC+GEP) in the Akaike’s information criteria (AIC) (297 vs 291), fit better with the Bayesian information criteria (BIC) (309 vs 313) and performed similarly with the Harrel’s C (0.86 (95% CI 0.80 to 0.91) vs 0.89 (0.84 to 0.94), respectively).ConclusionsCombination of GEP and LBD allows separation of patients into four easy-to-use prognostic groups and was similar to a model combining AJCC stage with GEP.

Diethyldithiocarbamate Treatment Up Regulates Manganese Superoxide Dismutase Gene Expression in Rat Liver

1996 ◽  
Vol 220 (3) ◽  
pp. 546-552 ◽  
Author(s):  
Silvia Borrello ◽  
Maria Emilia De Leo ◽  
Matteo Landriscina ◽  
Bernardetta Palazzotti ◽  
Tommaso Galeotti
Keyword(s):  
Gene Expression ◽  
Superoxide Dismutase ◽  
Rat Liver ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide ◽  
Superoxide Dismutase Gene
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