Beneficial effect of 20-hydroxyecdysone exerted by modulating antioxidants and inflammatory cytokine levels in collagen-induced arthritis: A model for rheumatoid arthritis

Brazilian propolis AF-08 as a dietary supplement has been shown to be effective in alleviating symptoms of herpes simplex virus and respiratory syncytial virus infection in mice. The alleviation was associated with the modification of immunological activity by AF-08 as a cytokine regulator. The immunomodulatory activity of AF-08 was suggested to contribute to the severity of symptoms and pathogenesis in cytokine-mediated diseases. In this study, we investigated the effectiveness of AF-08 as a potential cytokine modulator in alleviating autoimmune diseases. The efficacy and cytokine-modulatory activity of AF-08 were examined in collagen-induced arthritis (CIA) in mice. Mice were immunized with type II collagen. AF-08 at 0, 30, or 100 mg/kg was administered orally to the immunized mice once daily for three weeks before and/or three times daily for two weeks after the onset of CIA. The development of arthritis of the paws and inflammatory cytokine levels in serum were examined. AF-08 at 100 mg/kg significantly reduced the incidence and severity of CIA prophylactically and therapeutically and reduced the rise of systemic interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α levels in the early phase of CIA. In the early phase of CIA, the reduction of inflammatory cytokine levels by AF-08 correlated with the amelioration of symptoms of CIA. AF-08 might inhibit the initiation of cytokine-mediated disease rather than suppressing disease progression without toxicity. AF-08 was confirmed to possess cytokine-modulatory activity in vivo. It is possible that AF-08 is a potential prophylactic and therapeutic agent for autoimmune diseases.

Download Full-text

Changes in aqueous and vitreous inflammatory cytokine levels in neovascular age-related macular degeneration: A systematic review and meta-analysis

10.26226/morressier.5e61463c250f7ee26aeb41ed ◽

2020 ◽

Author(s):

Samuel Minaker

Keyword(s):

Systematic Review ◽

Macular Degeneration ◽

Inflammatory Cytokine ◽

Meta Analysis ◽

Age Related Macular Degeneration ◽

Age Related ◽

Cytokine Levels

Download Full-text

THE EFFECT OF SUBACUTE INTOXICATION WITH ETHYLENE CHLOROHYDRIN ON IMMUNE RESPONSES, TH1 AND TH2 LYMPHOCYTE FUNCTION AND CYTOKINE LEVELS IN RATS BLOOD

Токсикологический вестник ◽

10.36946/0869-7922-2017-3-27-30 ◽

2017 ◽

pp. 27-30

Author(s):

P. F. Zabrodskii ◽

V. V. Maslyakov ◽

M. S. Gromov

Keyword(s):

Immune Responses ◽

Inflammatory Cytokine ◽

Albino Rats ◽

Lymphocyte Function ◽

Ethylene Chlorohydrin ◽

Immunoregulatory Cytokines ◽

Cytokine Levels ◽

Th1 And Th2 Lymphocytes ◽

Cellular Immune ◽

Th1 And Th2

In experiments on outbred albino rats, it was established that subacute intoxication with ethylene chlorohydrin (0.2 LD50 daily for 4 days) causes a decrease in Th1 and Th2 lymphocytes function to the same extent, diminishes parameters of humoral and cellular immune responses and the content of immunoregulatory cytokines IFN- , IL-2, IL-4 in blood, increases concentrations of pro-inflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10.

Download Full-text

Surfactant protein A reduces TLR4 and inflammatory cytokine mRNA levels in neonatal mouse ileum

Scientific Reports ◽

10.1038/s41598-021-82219-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Lidan Liu ◽

Chaim Z. Aron ◽

Cullen M. Grable ◽

Adrian Robles ◽

Xiangli Liu ◽

...

Keyword(s):

Proinflammatory Cytokine ◽

Inflammatory Cytokine ◽

Protein A ◽

Surfactant Protein ◽

Surfactant Protein A ◽

Mrna Levels ◽

Wild Type ◽

Cytokine Mrna ◽

Protein Levels ◽

Cytokine Levels

AbstractLevels of intestinal toll-like receptor 4 (TLR4) impact inflammation in the neonatal gastrointestinal tract. While surfactant protein A (SP-A) is known to regulate TLR4 in the lung, it also reduces intestinal damage, TLR4 and inflammation in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats. We hypothesized that SP-A-deficient (SP-A−/−) mice have increased ileal TLR4 and inflammatory cytokine levels compared to wild type mice, impacting intestinal physiology. We found that ileal TLR4 and proinflammatory cytokine levels were significantly higher in infant SP-A−/− mice compared to wild type mice. Gavage of neonatal SP-A−/− mice with purified SP-A reduced ileal TLR4 protein levels. SP-A reduced expression of TLR4 and proinflammatory cytokines in normal human intestinal epithelial cells (FHs74int), suggesting a direct effect. However, incubation of gastrointestinal cell lines with proteasome inhibitors did not abrogate the effect of SP-A on TLR4 protein levels, suggesting that proteasomal degradation is not involved. In a mouse model of experimental NEC, SP-A−/− mice were more susceptible to intestinal stress resembling NEC, while gavage with SP-A significantly decreased ileal damage, TLR4 and proinflammatory cytokine mRNA levels. Our data suggests that SP-A has an extrapulmonary role in the intestinal health of neonatal mice by modulating TLR4 and proinflammatory cytokines mRNA expression in intestinal epithelium.

Download Full-text