Mango ginger (curcuma amada) inhibits collagen-induced arthritis by modulating inflammatory cytokine levels in rats

Brazilian propolis AF-08 as a dietary supplement has been shown to be effective in alleviating symptoms of herpes simplex virus and respiratory syncytial virus infection in mice. The alleviation was associated with the modification of immunological activity by AF-08 as a cytokine regulator. The immunomodulatory activity of AF-08 was suggested to contribute to the severity of symptoms and pathogenesis in cytokine-mediated diseases. In this study, we investigated the effectiveness of AF-08 as a potential cytokine modulator in alleviating autoimmune diseases. The efficacy and cytokine-modulatory activity of AF-08 were examined in collagen-induced arthritis (CIA) in mice. Mice were immunized with type II collagen. AF-08 at 0, 30, or 100 mg/kg was administered orally to the immunized mice once daily for three weeks before and/or three times daily for two weeks after the onset of CIA. The development of arthritis of the paws and inflammatory cytokine levels in serum were examined. AF-08 at 100 mg/kg significantly reduced the incidence and severity of CIA prophylactically and therapeutically and reduced the rise of systemic interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α levels in the early phase of CIA. In the early phase of CIA, the reduction of inflammatory cytokine levels by AF-08 correlated with the amelioration of symptoms of CIA. AF-08 might inhibit the initiation of cytokine-mediated disease rather than suppressing disease progression without toxicity. AF-08 was confirmed to possess cytokine-modulatory activity in vivo. It is possible that AF-08 is a potential prophylactic and therapeutic agent for autoimmune diseases.

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Changes in aqueous and vitreous inflammatory cytokine levels in neovascular age-related macular degeneration: A systematic review and meta-analysis

10.26226/morressier.5e61463c250f7ee26aeb41ed ◽

2020 ◽

Author(s):

Samuel Minaker

Keyword(s):

Systematic Review ◽

Macular Degeneration ◽

Inflammatory Cytokine ◽

Meta Analysis ◽

Age Related Macular Degeneration ◽

Age Related ◽

Cytokine Levels

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THE EFFECT OF SUBACUTE INTOXICATION WITH ETHYLENE CHLOROHYDRIN ON IMMUNE RESPONSES, TH1 AND TH2 LYMPHOCYTE FUNCTION AND CYTOKINE LEVELS IN RATS BLOOD

Токсикологический вестник ◽

10.36946/0869-7922-2017-3-27-30 ◽

2017 ◽

pp. 27-30

Author(s):

P. F. Zabrodskii ◽

V. V. Maslyakov ◽

M. S. Gromov

Keyword(s):

Immune Responses ◽

Inflammatory Cytokine ◽

Albino Rats ◽

Lymphocyte Function ◽

Ethylene Chlorohydrin ◽

Immunoregulatory Cytokines ◽

Cytokine Levels ◽

Th1 And Th2 Lymphocytes ◽

Cellular Immune ◽

Th1 And Th2

In experiments on outbred albino rats, it was established that subacute intoxication with ethylene chlorohydrin (0.2 LD50 daily for 4 days) causes a decrease in Th1 and Th2 lymphocytes function to the same extent, diminishes parameters of humoral and cellular immune responses and the content of immunoregulatory cytokines IFN- , IL-2, IL-4 in blood, increases concentrations of pro-inflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10.

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Characterization of the genetic structure of mango ginger (Curcuma amada Roxb.) from Myanmar in farm and genebank collection by the neutral and functional genomic markers

Electronic Journal of Biotechnology ◽

10.2225/vol13-issue6-fulltext-10 ◽

2010 ◽

Vol 13 (6) ◽

pp. 0-0 ◽

Cited By ~ 2

Author(s):

Shakeel Ahmad Jatoi ◽

Akira Kikuchi ◽

Dawood Ahmad ◽

Kazuo N. Watanbe

Keyword(s):

Genetic Structure ◽

Functional Genomic ◽

Mango Ginger ◽

Curcuma Amada ◽

Genomic Markers ◽

Genebank Collection

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Surfactant protein A reduces TLR4 and inflammatory cytokine mRNA levels in neonatal mouse ileum

Scientific Reports ◽

10.1038/s41598-021-82219-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Lidan Liu ◽

Chaim Z. Aron ◽

Cullen M. Grable ◽

Adrian Robles ◽

Xiangli Liu ◽

...

Keyword(s):

Proinflammatory Cytokine ◽

Inflammatory Cytokine ◽

Protein A ◽

Surfactant Protein ◽

Surfactant Protein A ◽

Mrna Levels ◽

Wild Type ◽

Cytokine Mrna ◽

Protein Levels ◽

Cytokine Levels

AbstractLevels of intestinal toll-like receptor 4 (TLR4) impact inflammation in the neonatal gastrointestinal tract. While surfactant protein A (SP-A) is known to regulate TLR4 in the lung, it also reduces intestinal damage, TLR4 and inflammation in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats. We hypothesized that SP-A-deficient (SP-A−/−) mice have increased ileal TLR4 and inflammatory cytokine levels compared to wild type mice, impacting intestinal physiology. We found that ileal TLR4 and proinflammatory cytokine levels were significantly higher in infant SP-A−/− mice compared to wild type mice. Gavage of neonatal SP-A−/− mice with purified SP-A reduced ileal TLR4 protein levels. SP-A reduced expression of TLR4 and proinflammatory cytokines in normal human intestinal epithelial cells (FHs74int), suggesting a direct effect. However, incubation of gastrointestinal cell lines with proteasome inhibitors did not abrogate the effect of SP-A on TLR4 protein levels, suggesting that proteasomal degradation is not involved. In a mouse model of experimental NEC, SP-A−/− mice were more susceptible to intestinal stress resembling NEC, while gavage with SP-A significantly decreased ileal damage, TLR4 and proinflammatory cytokine mRNA levels. Our data suggests that SP-A has an extrapulmonary role in the intestinal health of neonatal mice by modulating TLR4 and proinflammatory cytokines mRNA expression in intestinal epithelium.

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Characterization of Volatile Organic Compounds in Mango Ginger (Curcuma amada Roxb.) from Myanmar

Metabolites ◽

10.3390/metabo11010021 ◽

2020 ◽

Vol 11 (1) ◽

pp. 21

Author(s):

Yanhang Chen ◽

Musavvara Kh. Shukurova ◽

Yonathan Asikin ◽

Miyako Kusano ◽

Kazuo N. Watanabe

Keyword(s):

Solid Phase ◽

Species Variation ◽

Mango Ginger ◽

Food Industries ◽

Curcuma Amada ◽

Alternative Medicines ◽

Flight Mass Spectrometry ◽

Volatile Organic ◽

Loss Of Appetite

Curcuma amada Roxb. (Zingiberaceae), commonly known as mango ginger because its rhizome and foliar parts have a similar aroma to mango. The rhizome has been widely used in food industries and alternative medicines to treat a variety of internal diseases such as cough, bronchitis, indigestion, colic, loss of appetite, hiccups, and constipation. The composition of the volatile constituents in a fresh rhizome of C. amada is not reported in detail. The present study aimed to screen and characterize the composition of volatile organic compound (VOC) in a fresh rhizome of three C. amada (ZO45, ZO89, and ZO114) and one C. longa (ZO138) accessions originated from Myanmar. The analysis was carried out by means of headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-time-of-flight-mass spectrometry (GC-TOF-MS). As a result, 122 VOCs were tentatively identified from the extracted 373 mass spectra. The following compounds were the ten most highly abundant and broadly present ones: ar-turmerone, α-zingiberene, α-santalene, (E)-γ-atlantone, cuparene, β-bisabolene, teresantalol, β-sesquiphellandrene, trans-α-bergamotene, γ-curcumene. The intensity of ar-turmerone, the sesquiterpene which is mainly characterized in C. longa essential oil (up to 15.5–27.5%), was significantly higher in C. amada accession ZO89 (15.707 ± 5.78a) compared to C. longa accession ZO138 (0.300 ± 0.08b). Cis-α-bergamotene was not detected in two C. amada accessions ZO45 and ZO89. The study revealed between-species variation regarding identified VOCs in the fresh rhizome of C. amada and C. longa.

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Acetylcholine-producing T cells augment innate immune-driven colitis but are redundant in T cell-driven colitis

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00067.2019 ◽

2019 ◽

Vol 317 (5) ◽

pp. G557-G568 ◽

Cited By ~ 2

Author(s):

Rose A. Willemze ◽

David J. Brinkman ◽

Olaf Welting ◽

Patricia H. P. van Hamersveld ◽

Caroline Verseijden ◽

...

Keyword(s):

Immune Response ◽

T Cells ◽

Nerve Stimulation ◽

Vagus Nerve Stimulation ◽

Inflammatory Cytokine ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Innate Immune ◽

Cytokine Levels ◽

Colitis Model

Clinical trials suggest that vagus nerve stimulation presents an alternative approach to classical immune suppression in Crohn's disease. T cells capable of producing acetylcholine (ChAT+ T cells) in the spleen are essential mediators of the anti-inflammatory effect of vagus nerve stimulation. Besides the spleen, ChAT+ T cells are found abundantly in Peyer’s patches of the small intestine. However, the role of ChAT+ T cells in colitis pathogenesis is unknown. Here, we made use of CD4creChATfl/fl mice (CD4ChAT−/− mice) lacking ChAT expression specifically in CD4+ T cells. Littermates (ChATfl/fl mice) served as controls. In acute dextran sulfate sodium (DSS)-induced colitis (7 days of 2% DSS in drinking water), CD4ChAT−/− mice showed attenuated colitis and lower intestinal inflammatory cytokine levels compared with ChATfl/fl mice. In contrast, in a resolution model of DSS-induced colitis (5 days of 2% DSS followed by 7 days without DSS), CD4ChAT−/− mice demonstrated a worsened colitis recovery and augmented colonic histological inflammation scores and inflammatory cytokine levels as compared with ChATfl/fl mice. In a transfer colitis model using CD4+CD45RBhigh T cells, T cells from CD4ChAT−/− mice induced a similar level of colitis compared with ChATfl/fl T cells. Together, our results indicate that ChAT+ T cells aggravate the acute innate immune response upon mucosal barrier disruption in an acute DSS-induced colitis model, whereas they are supporting the later resolution process of this innate immune-driven colitis. Surprisingly, ChAT expression in T cells seems redundant in the context of T cell-driven colitis. NEW & NOTEWORTHY By using different mouse models of experimental colitis, we provide evidence that in dextran sulfate sodium-induced colitis, ChAT+ T cells capable of producing acetylcholine worsen the acute immune response, whereas they support the later healing phase of this innate immune-driven colitis.

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