THE EFFECT OF SUBACUTE INTOXICATION WITH ETHYLENE CHLOROHYDRIN ON IMMUNE RESPONSES, TH1 AND TH2 LYMPHOCYTE FUNCTION AND CYTOKINE LEVELS IN RATS BLOOD

2017 ◽  
pp. 27-30
Author(s):  
P. F. Zabrodskii ◽  
V. V. Maslyakov ◽  
M. S. Gromov
Keyword(s):  
Immune Responses ◽  
Inflammatory Cytokine ◽  
Albino Rats ◽  
Lymphocyte Function ◽  
Ethylene Chlorohydrin ◽  
Immunoregulatory Cytokines ◽  
Cytokine Levels ◽  
Th1 And Th2 Lymphocytes ◽  
Cellular Immune ◽  
Th1 And Th2

In experiments on outbred albino rats, it was established that subacute intoxication with ethylene chlorohydrin (0.2 LD50 daily for 4 days) causes a decrease in Th1 and Th2 lymphocytes function to the same extent, diminishes parameters of humoral and cellular immune responses and the content of immunoregulatory cytokines IFN- , IL-2, IL-4 in blood, increases concentrations of pro-inflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10.

scholarly journals Combined Treatment of Adipose Derived-Mesenchymal Stem Cells and Pregabalin Is Superior to Monotherapy for the Treatment of Neuropathic Pain in Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Shimaa Mohammad Yousof ◽  
Doaa Attia ElSayed ◽  
Amani A. El-Baz ◽  
Hanaa S. Sallam ◽  
Faten Abbas
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Inflammatory Cytokine ◽  
Combined Treatment ◽  
Medial Gastrocnemius ◽  
Albino Rats ◽  
Cytokine Levels

Aims. Neuropathic pain following nerve injury does not respond well to most available pharmacological remedies. We aimed to compare the outcome of the addition of adipose-derived mesenchymal stem cells (ADMSCs) to pregabalin for neuropathic pain treatment. Methods. Adult female albino rats ( n = 100 ) were randomized to receive traumatic sciatic nerve injury or sham. Animals were then randomized to ADMSC treatment with or without pregabalin. We conducted a battery of neurobehavioral and electrophysiological to assess neuropathic pain. Following sacrifice, we evaluated the histological changes and gene expression of brain-derived neurotrophic factor (BDNF) in the sciatic nerve. Serum and sciatic nerve tissue pro- and inflammatory cytokine levels were also assessed. Results. (1) All treatments significantly improved thermal withdrawal latency, sciatic nerve conduction velocity, and proinflammatory cytokine levels in injured animals, with no significant effect of the combined treatments compared to pregabalin monotherapy ( p < 0.05 each). (2) Combined treatment significantly improved medial gastrocnemius electromyographic amplitude and sciatic function index compared to pregabalin monotherapy ( p < 0.05 each). (3) Combined treatment significantly increased the BDNF expression, decreased anti-inflammatory cytokine ( p < 0.05 each), and restored the structural nerve damage, compared to pregabalin monotherapy. Conclusions. Combined treatment is associated with greater improvement of the sciatic nerve structure and function. Further studies are warranted to study the mechanism of action of the combined treatment to improve neuropathic pain.

Comparative analysis of cellular immune responses and cytokine levels in sheep experimentally infected with bluetongue virus serotype 1 and 8

2015 ◽  
Vol 177 (1-2) ◽  
pp. 95-105 ◽  
Author(s):  
P.J. Sánchez-Cordón ◽  
A.C. Pérez de Diego ◽  
J.C. Gómez-Villamandos ◽  
J.M. Sánchez-Vizcaíno ◽  
F.J. Pleguezuelos ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Immune Responses ◽  
Bluetongue Virus ◽  
Cellular Immune Responses ◽  
Virus Serotype ◽  
Cytokine Levels ◽  
Serotype 1 ◽  
Cellular Immune

scholarly journals In Vitro Effects of an Immunostimulating Bacterial Lysate on Human Lymphocyte Function

2005 ◽  
Vol 18 (2) ◽  
pp. 245-254 ◽  
Author(s):  
G. Lanzilli ◽  
R. Falchetti ◽  
M. Tricarico ◽  
D. Ungheri ◽  
M.P. Fuggetta
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
Respiratory Tract ◽  
Human Lymphocyte ◽  
Lymphocyte Function ◽  
Cellular Immune Responses ◽  
Competent Cells ◽  
In Vitro Effects ◽  
Cellular Immune

MLBL is an oral immunostimulating vaccine consisting of bacterial standardized lysates obtained by mechanical lysis of different strains of Gram-positive and Gram-negative bacteria that can cause acute and chronic infections of the respiratory tract. Previous studies suggested a stimulating effect of MLBL both on humoral and cellular immune responses. In the present study, the in vitro effects of MLBL on human lymphocyte effector functions and its mechanisms of action were evaluated. The results show that the most remarkable effects of MLBL on the immune system are: i) activation of the IL-2 receptor (IL-2Rα) on different lymphocyte subsets (B, CD4+ T and CD8+ T cells) involved both in humoral and cellular immune responses; ii) induction of cytokine synthesis (IL-2, IL-10, IL-12, IFNγ) in the immune competent cells that induce and regulate immune responses; iii) generation of CD4+ and CD8+ effector T cells. Overall, these results suggest that the therapeutic effect of MLBL on acute and recurrent infections of the respiratory tract is related to its ability to activate the responses of different subsets of immune competent cells both for humoral and cellular immunity. Moreover, these effects can be induced either by direct immune cell activation or through the generation and activation of immune effector cells.

scholarly journals Cellular Immune Responses in Humans Induced by Two Serogroup B Meningococcal Outer Membrane Vesicle Vaccines Given Separately and in Combination

2016 ◽  
Vol 23 (4) ◽  
pp. 353-362 ◽  
Author(s):  
Fredrik Oftung ◽  
Gro Ellen Korsvold ◽  
Audun Aase ◽  
Lisbeth M. Næss
Keyword(s):  
T Cell ◽  
Immune Responses ◽  
Membrane Vesicle ◽  
Cross Reactivity ◽  
Outer Membrane Vesicle ◽  
Th2 Cytokines ◽  
Cellular Immune Responses ◽  
Th1 And Th2 Cytokines ◽  
Cellular Immune ◽  
Th1 And Th2

ABSTRACTMenBvac and MeNZB are safe and efficacious outer membrane vesicle (OMV) vaccines against serogroup B meningococcal disease. Antibody responses have previously been investigated in a clinical trial with these two OMV vaccines given separately (25 μg/dose) or in combination (12.5 and 12.5 μg/dose) in three doses administered at 6-week intervals. Here, we report the results from analyzing cellular immune responses against MenBvac and MeNZB OMVs in terms of antigen-specific CD4+T cell proliferation and secretion of cytokines. The proliferative CD4+T cell responses to the combined vaccine were of the same magnitude as the homologous responses observed for each individual vaccine. The results also showed cross-reactivity in the sense that both vaccine groups receiving separate vaccines responded to both homologous and heterologous OMV antigen when assayed for antigen-specific cellular proliferation. In addition, a multiplex bead array assay was used to analyze the presence of Th1 and Th2 cytokines in cell culture supernatants. The results showed that gamma interferon, interleukin-4 (IL-4), and IL-10 responses could be detected as a result of vaccination with both the MenBvac and the MeNZB vaccines given separately, as well as when given in combination. With respect to cross-reactivity, the cytokine results paralleled the observations made for proliferation. In conclusion, the results demonstrate that cross-reactive cellular immune responses involving both Th1 and Th2 cytokines can be induced to the same extent by different tailor-made OMV vaccines given either separately or in combination with half the dose of each vaccine.

Sign in / Sign up

Export Citation Format

Share Document