scholarly journals Integrative taxonomy uncovers a new stygobiotic Caridina species (Decapoda, Caridea, Atyidae) from Guizhou Province, China

ZooKeys ◽  
2021 ◽  
Vol 1028 ◽  
pp. 29-47
Author(s):  
Shuo Feng ◽  
Qing-Hua Chen ◽  
Zhao-Liang Guo
Keyword(s):  
Abdominal Segment ◽  
Dna Analysis ◽  
Integrative Taxonomy ◽  
Guizhou Province ◽  
Mitochondrial Coi ◽  
Needed Information ◽  
Appendix Masculina ◽  
Unique Shape ◽  
Cave Ecosystems ◽  
Distribution And Ecology

Collecting much-needed information on the taxonomy, distribution, and ecology of cave-dwelling shrimp is vital for addressing the urgent challenges in conservation biodiversity in fragile cave ecosystems. Caridina incolorsp. nov., a new atyid shrimp from an underground stream of Yaoshui Cave, Daqikong scenic area, Libo County, Guizhou Province, southwestern China is described based on morphology and DNA analysis (mitochondrial COI). Caridina incolorsp. nov. differs from epigean congeners by its smaller eyes which range from reduced to completely blind; colorless body and appendages; long stylocerite and sixth abdominal segment; and relatively large eggs. In comparison to other cave species, Caridina incolorsp. nov. presents a long rostrum and stylocerite; slender sixth abdominal segment; and unique shape of the appendix masculina. Data on the habitat, ecology, and levels of threat are provided and suggest that it should be categorized as Critically Endangered (CR) under the current IUCN criteria.

scholarly journals Utilizing integrative taxonomy uncovers a new stygobiotic Caridina species (Decapoda: Caridea: Atyidae) from Guizhou Province, China

2021 ◽  
Author(s):  
Shuo Feng ◽  
Qing-Hua Chen ◽  
Zhao-Liang Guo
Keyword(s):  
Abdominal Segment ◽  
Dna Analysis ◽  
Integrative Taxonomy ◽  
Critically Endangered ◽  
Guizhou Province ◽  
Mitochondrial Coi ◽  
Needed Information ◽  
Appendix Masculina ◽  
Unique Shape ◽  
Distribution And Ecology

Collecting much-needed information on the taxonomy, distribution, and ecology of cave-dwelling shrimp is the foundation for addressing urgent challenges in conservation biodiversity and these fragile ecosystems. Caridina unicolor sp. nov., a new atyid shrimp from an underground stream of Yaoshui Cave, Daqikong scenic area, Libo County, Guizhou Province, southwestern China is described based on morphology and DNA analysis (mitochondrial COI). Caridina unicolor sp. nov. differs from epigean congeners by the variable eye, ranging from reduced to blind; colorless body and appendages; the long stylocerite and sixth abdominal segment; and relatively large eggs. In comparison to other cave species, Caridina unicolor sp. nov. presents a long rostrum and stylocerite; the slender sixth abdominal segment; and the unique shape of appendix masculina. The data on the habitat, ecology, and the levels of threat are provided and suggest that it be categorized as Critically Endangered (CR).

scholarly journals Caridina sinanensis, a new species of stygobiotic atyid shrimp (Decapoda, Caridea, Atyidae) from a karst cave in the Guizhou Province, southwestern China

ZooKeys ◽  
2020 ◽  
Vol 1008 ◽  
pp. 17-35
Author(s):  
Da-Jian Xu ◽  
Deng-Xu Li ◽  
Xiao-Zhuang Zheng ◽  
Zhao-Liang Guo
Keyword(s):  
New Species ◽  
Guizhou Province ◽  
Southwestern China ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Biodiversity Survey ◽  
Pigment Spot ◽  
Mitochondrial Coi ◽  
Appendix Masculina ◽  
A New Species

From a biodiversity survey of a subterranean habitat near Sinan County, Guizhou Province, southwestern China, a new atyid shrimp of the genus Caridina H. Milne Edwards, 1837, C. sinanensissp. nov. was discovered. The new species can be separated from other congeners based on a combination of characters including depigmentation in body and reduction of eyes, small pigment spot at the centre of the cornea, the shape of rostrum and the endopod of the 1st male pleopod, and the relatively longer appendix interna on the appendix masculina of the 2nd pleopod. Mitochondrial COI and 16S rRNA gene sequences also support the establishment of the new species. Information on the habitat, and the levels of threat are discussed to guide the conservation of C. sinanensissp. nov.

scholarly journals Looking at the Nudibranch Family Myrrhinidae (Gastropoda, Heterobranchia) from a Mitochondrial ‘2D Folding Structure’ Point of View

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 583
Author(s):  
Giulia Furfaro ◽  
Paolo Mariottini
Keyword(s):  
16S Rrna ◽  
Phylogenetic Analyses ◽  
Integrative Taxonomy ◽  
Point Of View ◽  
Past Study ◽  
Mitochondrial Coi ◽  
The Past ◽  
Combining Data ◽  
Folding Structure ◽  
Comparison Of The Results

Integrative taxonomy is an evolving field of multidisciplinary studies often utilised to elucidate phylogenetic reconstructions that were poorly understood in the past. The systematics of many taxa have been resolved by combining data from different research approaches, i.e., molecular, ecological, behavioural, morphological and chemical. Regarding molecular analysis, there is currently a search for new genetic markers that could be diagnostic at different taxonomic levels and that can be added to the canonical ones. In marine Heterobranchia, the most widely used mitochondrial markers, COI and 16S, are usually analysed by comparing the primary sequence. The 16S rRNA molecule can be folded into a 2D secondary structure that has been poorly exploited in the past study of heterobranchs, despite 2D molecular analyses being sources of possible diagnostic characters. Comparison of the results from the phylogenetic analyses of a concatenated (the nuclear H3 and the mitochondrial COI and 16S markers) dataset (including 30 species belonging to eight accepted genera) and from the 2D folding structure analyses of the 16S rRNA from the type species of the genera investigated demonstrated the diagnostic power of this RNA molecule to reveal the systematics of four genera belonging to the family Myrrhinidae (Gastropoda, Heterobranchia). The “molecular morphological” approach to the 16S rRNA revealed to be a powerful tool to delimit at both species and genus taxonomic levels and to be a useful way of recovering information that is usually lost in phylogenetic analyses. While the validity of the genera Godiva, Hermissenda and Phyllodesmium are confirmed, a new genus is necessary and introduced for Dondice banyulensis, Nemesis gen. nov. and the monospecific genus Nanuca is here synonymised with Dondice, with Nanuca sebastiani transferred into Dondice as Dondice sebastiani comb. nov.

scholarly journals Species detections in environmental DNA using metabarcoding: a useful tool in estuaries monitoring

2015 ◽  
Author(s):  
Adrián Mártinez-Marqués ◽  
Carlos Enrique Carleos ◽  
Eva García-Vazquez ◽  
Yaisel J. Borrell Pichs
Keyword(s):  
Dna Analysis ◽  
Anthropogenic Activities ◽  
Dna Barcode ◽  
Environmental Dna ◽  
Coi Gene ◽  
Universal Primers ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Cantabrian Coast ◽  
Taxonomic Groups

Estuaries are amongst the most productive habitats in Earth, producing more organic materia than forests, meadows or agricultural lands. In addition, estuaries exhibit high, and precious, biodiversity levels. In this study an environmental DNA analysis of the two most important estuaries in Asturias (Cantabrian Coast, north Iberia) in terms of food production (Ría del Eo and Ría de Villaviciosa) was carried out. The objective was to monitor aquatic biodiversity and also to detect alien species that can be associated with anthropogenic activities (e.g.: aquaculture). To achieve these objectives, a metabarcoding methodology based in NGS (next generation sequencing) and the mitochondrial COI gene as a DNA Barcode was used. Results showed that this methodology was useful to detect the presence of three different non-native genera (Crepidula, Lymnaea, Macrobrachium) that are probably parasitating species cultured in these estuaries. It is true that Metabarcoding has still unsolved problems such as the lack of 100% universal primers and paucity of referenced sequences for some taxonomic groups in the databases. However, it represents already a powerful tool to manage the resources of these important ecosystems and to guarantee their long-term sustainailibity.

A new species of palaemonid prawn Macrobrachium ramae sp.nov. (Malacostraca:Decapoda:Palaemonidae) from Rupnarayana River, West Bengal, India with its molecular profiles

Zootaxa ◽  
2021 ◽  
Vol 4952 (3) ◽  
pp. 540-550
Author(s):  
MITALI DAS ◽  
PRITI RANJAN PAHARI ◽  
TANMAY BHATTACHARYA
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
West Bengal ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Mitochondrial Coi ◽  
Appendix Masculina ◽  
Molecular Profiles ◽  
A New Species ◽  
Scanning Electron

A new species Macrobrachium ramae is described from Rupnarayana river, West Bengal, India along with its molecular characterization and Scanning electron microscopy. The species shares certain characters with M.gurudeve, M.jayasreei, M.kunjuramani and M.saengphani but differs remarkably from these species in the structure and shape of rostrum, telson, appendix masculina and in the size of the proximal segment of the antennular peduncle. Molecular characterization and phylogenetic analysis of M.ramae with mitochondrial COI and 16S rRNA genes reinforce the morphological conclusion and supports the view that it is a new species. 

Species status of Centrolene lema Duellman and Señaris, 2003 (Amphibia: Centrolenidae) revealed by Integrative Taxonomy

Zootaxa ◽  
2009 ◽  
Vol 1980 (1) ◽  
pp. 16-28 ◽  
Author(s):  
SANTIAGO CASTROVIEJO-FISHER ◽  
JUAN M. GUAYASAMIN ◽  
PHILIPPE J.R. KOK
Keyword(s):  
Integrative Taxonomy ◽  
Species Status ◽  
Distribution And Ecology

We evaluate the hypothesis of Centrolene lema as a species distinct from C. gorzulae using morphological, bioacoustic and genetic comparisons. Our results show that there are no consistent differences in any of these three areas; hence, we present C. lema as a synonym of C. gorzulae. Additionally we provide new data on the distribution and ecology of the species.

A new Ctenothrips from southwestern China (Thysanoptera: Thripidae)

Zootaxa ◽  
2011 ◽  
Vol 2926 (1) ◽  
pp. 65 ◽  
Author(s):  
YONGHUI XIE ◽  
HONGRUI ZHANG ◽  
ZHENGYUE LI
Keyword(s):  
New Species ◽  
North America ◽  
Body Surface ◽  
Type Species ◽  
Abdominal Segment ◽  
Guizhou Province ◽  
Type Specimens ◽  
Alpine Regions ◽  
Holarctic Region ◽  
Eleven Species

The genus Ctenothrips (Thysanoptera, Thripidae) was proposed by Franklin in 1907 with C. bridwelli as the type species from North America (Franklin, 1907). Up to now, eleven species have been known, all from the Holarctic region (Mound, 2011; Haga & Okajima, 1989; Kudo, 1977; Bhatti, 1976), and six of these have been described from China (Chen, 1979; Feng et al., 2003; Tong & Zhang, 1992). Though related to one of the most popular genera, Taeniothrips Amyot and Serville, by having similar head and antennae, Ctenothrips is distinctly characterized with polygonally reticulated body surface of abdomen and tube-like abdominal segment X. Species of the genus Ctenothrips have been found mainly in alpine regions, but we collected the new species described here from an area only as high as altitude of 900m in the southwest of China. In this paper, C. guizhouensis sp. n., is added as the twelfth member to this genus, and an identification key is provided to all 12 species, based mainly on descriptions. The new species was collected in the flowers of a species of Galium (Rubiaceae) at the Donggong temple, Zunyi City, Guizhou Province. The type specimens are preserved in Yunnan Agricultural University.

scholarly journals Revalidation of Oliera Brèthes (Lepidoptera: Cecidosidae) based on a redescription of O. argentinana and DNA analysis of Neotropical cecidosids

Zootaxa ◽  
2012 ◽  
Vol 3557 (1) ◽  
pp. 1 ◽  
Author(s):  
GILSON R. P. MOREIRA ◽  
GISLENE L. GONÇALVES ◽  
RODRIGO P. ELTZ ◽  
GERMÁN SAN BLAS ◽  
DONALD R. DAVIS
Keyword(s):  
Electron Microscopy ◽  
Dna Sequences ◽  
Dna Analysis ◽  
Morphological Characteristics ◽  
Rio Grande ◽  
Immature Stages ◽  
Rio Grande Do Sul ◽  
Mitochondrial Coi ◽  
The Family ◽  
Plant Galls

Larvae of Oliera argentinana, Brèthes 1916 (Lepidoptera: Cecidosidae) were rediscovered inducing spindle-shaped gallsenclosed within swollen stems of Schinus (Anacardiaceae) in central Argentina and Rio Grande do Sul, the southernmoststate of Brazil. Male, female, immature stages, and plant galls of O. argentinana are redescribed, using optical andscanning electron microscopy. The genus Oliera Brèthes, 1916, previously a junior synonym of Cecidoses Curtis, 1835,is revalidated, by comparing morphological characteristics within the family and through an analysis of mitochondrial(COI) DNA sequences, including putative members of the four Neotropical cecidosid genera. Information on preliminary Cecidosidae phylogeny and taxonomy is also provided.

scholarly journals Species detections in environmental DNA using metabarcoding: a useful tool in estuaries monitoring

2015 ◽  
Author(s):  
Adrián Mártinez-Marqués ◽  
Carlos Enrique Carleos ◽  
Eva García-Vazquez ◽  
Yaisel J. Borrell Pichs
Keyword(s):  
Dna Analysis ◽  
Anthropogenic Activities ◽  
Dna Barcode ◽  
Environmental Dna ◽  
Coi Gene ◽  
Universal Primers ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Cantabrian Coast ◽  
Taxonomic Groups

Estuaries are amongst the most productive habitats in Earth, producing more organic materia than forests, meadows or agricultural lands. In addition, estuaries exhibit high, and precious, biodiversity levels. In this study an environmental DNA analysis of the two most important estuaries in Asturias (Cantabrian Coast, north Iberia) in terms of food production (Ría del Eo and Ría de Villaviciosa) was carried out. The objective was to monitor aquatic biodiversity and also to detect alien species that can be associated with anthropogenic activities (e.g.: aquaculture). To achieve these objectives, a metabarcoding methodology based in NGS (next generation sequencing) and the mitochondrial COI gene as a DNA Barcode was used. Results showed that this methodology was useful to detect the presence of three different non-native genera (Crepidula, Lymnaea, Macrobrachium) that are probably parasitating species cultured in these estuaries. It is true that Metabarcoding has still unsolved problems such as the lack of 100% universal primers and paucity of referenced sequences for some taxonomic groups in the databases. However, it represents already a powerful tool to manage the resources of these important ecosystems and to guarantee their long-term sustainailibity.

scholarly journals Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

2020 ◽  
Author(s):  
Daniel Stec ◽  
Denis T. Tumanov ◽  
Reinhardt Møbjerg Kristensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
Morphometric Analyses ◽  
Taxonomic Methods ◽  
Two Populations ◽  
Scanning Electron

In this paper we describe Macrobiotus engbergi sp. nov. and Tenuibiotus zandrae sp. nov. from Greenland. Our study has involved both classical taxonomic methods, which include morphological and morphometric analyses conducted with the use of light and scanning electron microscopy, and genetic analysis based on four molecular markers (three nuclear: 18S rRNA, 28S rRNA, ITS-2, and one mitochondrial: COI). Moreover, we re-examined the type series of Tenuibiotus voronkovi (Tumanov, 2007) as well as the original sample where the species was found and we provide new morphological data from light and scanning electron microscopy which enabled us to amend its description. Finally, we also analysed slides with animals and egg of two populations from Nordaustlandet and Edgeøya (archipelago of Svalbard, Norway) designated as T. voronkovi within its recent redescription. The results and comparisons presented in our study question the validity of this designation. 

Sign in / Sign up

Export Citation Format

Share Document