scholarly journals Three new species of Heteromastus (Annelida, Capitellidae) from Korean waters, with genetic evidence based on two gene markers

ZooKeys ◽  
2019 ◽  
Vol 869 ◽  
pp. 1-18 ◽  
Author(s):  
Man-Ki Jeong ◽  
Ho Young Soh ◽  
Hae-Lip Suh
Keyword(s):  
Dna Sequences ◽  
Staining Pattern ◽  
Genetic Difference ◽  
Public Database ◽  
Gene Markers ◽  
The Public ◽  
Korean Waters ◽  
Comprehensive Comparison ◽  
Distinct Node ◽  
Three New Species

Three undescribed species of Heteromastus Eisig, 1887 were collected from intertidal to sublittoral habitats in western and southern waters of Korea. Heteromastus namhaensis sp. nov. is distinguishable from other congeners by the presence of hemispheric notopodial lobes in the posterior abdomen. Heteromastus gusipoensis sp. nov. closely resembles H. tohbaiensis Yabe & Mawatari, 1998 in the absence of posteriorly extended abdominal notopodial lobes, but differs in the absence of eyespots on the prostomium and distinct node on the shaft of thoracic hooks in H. gusipoensis. Heteromastus koreanus sp. nov. is similar to H. filiformis sensu Hutchings & Rainer, 1982 in the shape of abdominal notopodia, but clearly differs in dentition of the abdominal hooks and methylene green staining pattern (MGSP). DNA sequences (mtCOI and histone H3) of these new Korean species were compared with all sequences of Heteromastus species available in the public database. Molecular results showed distinct genetic differences among these three new Korean species at species level. Comparison of mtCOI gene revealed significant genetic difference between H. filiformis and these Korean species. A comprehensive comparison between three Heteromastus species of present study and their closely related congeners is conducted based on morphological and genetic results.

scholarly journals A new Notomastus (Annelida, Capitellidae) species from Korean waters, with genetic comparison based on three gene markers

ZooKeys ◽  
2018 ◽  
Vol 754 ◽  
pp. 141-155 ◽  
Author(s):  
Man-Ki Jeong ◽  
Ho Young Soh ◽  
Jin Hee Wi ◽  
Hae-Lip Suh
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Histone H3 ◽  
Similar Species ◽  
Gene Markers ◽  
Korean Waters ◽  
Muddy Bottom ◽  
Comprehensive Comparison ◽  
A New Species ◽  
Genetic Comparison

Notomastuskoreanussp. n., collected from the sublittoral muddy bottom of Korean waters, is described as a new species. The Korean new species closely resembles N.torquatus Hutchings & Rainer, 1979 in the chaetal arrangement and the details of abdominal segments, but differs in the position of genital pores and the absence of eyes. DNA sequences (mtCOI, 16S rRNA, and histone H3) of the new species were compared with all the available sequences of Notomastus species in the GenBank database. Three genes showed significant genetic differences between the new species and its congeners (COI: 51.2%, 16S: 38.1–47.3%, H3: 3.7–9.3%). This study also includes a comprehensive comparison of the new Korean Notomastus species with its most closely similar species, based on the morphological and genetic results.

scholarly journals CaptureSeq: Hybridization-Based Enrichment of cpn60 Gene Fragments Reveals the Community Structures of Synthetic and Natural Microbial Ecosystems

2021 ◽  
Vol 9 (4) ◽  
pp. 816
Author(s):  
Matthew G. Links ◽  
Tim J. Dumonceaux ◽  
E. Luke McCarthy ◽  
Sean M. Hemmingsen ◽  
Edward Topp ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Dna Sequences ◽  
Pcr Amplification ◽  
Shotgun Sequencing ◽  
Natural Ecosystems ◽  
Gene Markers ◽  
Microbial Profile ◽  
Microbial Ecosystems ◽  
Pcr Targeting

Background. The molecular profiling of complex microbial communities has become the basis for examining the relationship between the microbiome composition, structure and metabolic functions of those communities. Microbial community structure can be partially assessed with “universal” PCR targeting taxonomic or functional gene markers. Increasingly, shotgun metagenomic DNA sequencing is providing more quantitative insight into microbiomes. However, both amplicon-based and shotgun sequencing approaches have shortcomings that limit the ability to study microbiome dynamics. Methods. We present a novel, amplicon-free, hybridization-based method (CaptureSeq) for profiling complex microbial communities using probes based on the chaperonin-60 gene. Molecular profiles of a commercially available synthetic microbial community standard were compared using CaptureSeq, whole metagenome sequencing, and 16S universal target amplification. Profiles were also generated for natural ecosystems including antibiotic-amended soils, manure storage tanks, and an agricultural reservoir. Results. The CaptureSeq method generated a microbial profile that encompassed all of the bacteria and eukaryotes in the panel with greater reproducibility and more accurate representation of high G/C content microorganisms compared to 16S amplification. In the natural ecosystems, CaptureSeq provided a much greater depth of coverage and sensitivity of detection compared to shotgun sequencing without prior selection. The resulting community profiles provided quantitatively reliable information about all three domains of life (Bacteria, Archaea, and Eukarya) in the different ecosystems. The applications of CaptureSeq will facilitate accurate studies of host-microbiome interactions for environmental, crop, animal and human health. Conclusions: cpn60-based hybridization enriched for taxonomically informative DNA sequences from complex mixtures. In synthetic and natural microbial ecosystems, CaptureSeq provided sequences from prokaryotes and eukaryotes simultaneously, with quantitatively reliable read abundances. CaptureSeq provides an alternative to PCR amplification of taxonomic markers with deep community coverage while minimizing amplification biases.

scholarly journals ASTUDYON THERECORDOF EVALUATINGTHE SUPPLIERSACCORDING TO THE CONCEPT OF QUALITY AND BENEFITING FROM IT IN PRACTICE IN AL-FURAT STATE COMPANY AND FINDING POSSIBLE PROPOSALS FOR ITS DEVELOPMENT

2020 ◽  
Vol 12 (2) ◽  
pp. 81-92
Author(s):  
Ashwaq Abdul-AmeerNsiaf ◽  
Keyword(s):  
Quality Management ◽  
Quality Management System ◽  
International Standards ◽  
Iso 9001 ◽  
Public Database ◽  
Proper Selection ◽  
Public Companies ◽  
The Public ◽  
On Demand ◽  
Selection Of

Success in selecting the best among the sources of supply is one of the most important factors in the efficiency of the procurement activity in the company, because the proper selection of the source of the supply significantly affect the achievement of what is desired by the factors of quality, quantity, price and service, and the ability of the competent supplier to meet everything associated with this Factors of commitments, hereby supporting the procurement function's efforts to fully discharge its responsibilities, and in view of adopting of Al-Furat company quality management system by applying the standards of ISO (9001: 2015) and purpose of getting the on-demand benefits from the application of international standards regarding record of evaluating the suppliers Therefore the research addresses to studying the reality of the suppliers' record in evaluation accordance with the quality concept in Al-Furat Sate Company for Chemical and Pesticide Industries,proposals for its development through evaluating the suppliers on realistic and logical basis and activating it in practice and a proposal to generalize the evaluation of Contractors suppliers to the public companies affiliated to the Ministry of Industry and Minerals and other bodies through coordination with the Ministry of Planning and to provide a public database officially approved on the official sites of the above authorities, allowing any entity to benefit from the evaluation of suppliers by withdrawing an evaluation form and utilize the software available to prepare the database for the Supplier Valuation Register.

scholarly journals Three new species of the genus Coddingtonia from Asia (Araneae, Theridiosomatidae)

ZooKeys ◽  
2019 ◽  
Vol 886 ◽  
pp. 113-126
Author(s):  
Chengcheng Feng ◽  
Yucheng Lin
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Morphological Characters ◽  
Molecular Data ◽  
Current Paper ◽  
Distribution Map ◽  
Three New Species

The current paper expands knowledge of the genus Coddingtonia Miller, Griswold & Yin, 2009. Based on morphological characters and molecular data, three species are documented as new to science: C. erhuan Feng & Lin, sp. nov. (♀) from China, C. lizu Feng & Lin, sp. nov. (♀) from China, and C. huifengi Feng & Lin, sp. nov. (♂♀) from Indonesia. The type of C. euryopoides Miller, Griswold & Yin, 2009 is also reexamined. DNA sequences (COI), detailed illustrations of habitus, male palp and epigyne are provided for these four species, as well as a key and a distribution map for Coddingtonia species.

scholarly journals Three new species of leaflitter frogs from the upper Amazon forests: cryptic diversity within Pristimantis "ockendeni" (Anura: Strabomantidae) in Ecuador

Zootaxa ◽  
2008 ◽  
Vol 1784 (1) ◽  
pp. 11 ◽  
Author(s):  
KATHRYN R. ELMER ◽  
DAVID C. CANNATELLA
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Amazon Basin ◽  
Single Species ◽  
Cryptic Diversity ◽  
Morphological Differences ◽  
Three New Species ◽  
Multiple Species

We describe three new species of Pristimantis leaflitter frogs from the upper Amazon basin of Ecuador: Pristimantis achuar, Pristimantis altamnis, and Pristimantis kichwarum. Each of these new species was previously considered to be part of a single species, formerly known as Eleutherodactylus ockendeni (Boulenger) morph B. However, analysis of DNA sequences together with examination of the morphology of more than 600 specimens from Ecuador reveals that multiple species were confused under the same name. The morphological differences among the three new species are subtle but consistent: presence or absence of a black canthal stripe and/or supratympanic ridge, snout-vent length, and ventral colouration. The three species are generally allopatric and geographically restricted, although at some localities two of the species occur sympatrically.Describimos tres especies nuevas de ranas del sotobosque de la cuenca Amazónica Ecuatoriana: Pristimantis achuar, Pristimantis altamnis y Pristimantis kichwarum. Estas especies fueron consideradas antes como una sola especie bajo el nombre Eleutherodactylus ockendeni (Boulenger) morfo B. Sin embargo, el análisis de secuencias de ADN y la examinación de la morfología de más que 600 especímenes Ecuatorianos indican que en realidad existen tres especies. Sus diferencias morfológicas son sutiles pero constantes: presencia o ausencia de una raya cantal negra y/o de un pliegue supratimpánico y su coloración ventral. Las tres especies tienen distribuciones alopátricas y distintas aunque en algunas localidades se puede encontrar dos de las tres especies juntas.

scholarly journals Acalyptris Meyrick: revision of the platani and staticis groups in Europe and the Mediterranean (Lepidoptera: Nepticulidae)

Zootaxa ◽  
2007 ◽  
Vol 1436 (1) ◽  
pp. 1 ◽  
Author(s):  
ERIK J. VAN NIEUKERKEN
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Mediterranean Species ◽  
Mt Dna ◽  
Pupal Exuviae ◽  
European Species ◽  
Group A ◽  
The Mediterranean ◽  
Three New Species

The European and Mediterranean species of the Acalyptris platani and A. staticis groups are revised, with respectively four and six species. Three new species are described: A. pistaciae Van Nieukerken sp. n. (from Greece, Cyprus and Turkey, on Pistacia) in the platani group, A. limoniastri Van Nieukerken sp. n. (from Algeria and Tunisia, associated with Limoniastrum) and A. lesbia Van Nieukerken & Hull sp. n. (from Greece: Lesvos, on Limonium gmelini) in the staticis group. Lectotypes are selected for A. minimella (Rebel, 1924) and A. staticis (Walsingham, 1908). The European species of the A. platani group are leafminers on Anacardiaceae, Platanaceae and Loranthaceae, the species of the A. staticis group feed on Plumbaginaceae, except A. pyrenaica A. & Z. Laštůvka, for which the host is unknown, but likely to belong to another family. Immatures are described for five species, final instar larvae and pupal exuviae for all species in the A. platani group and for A. lesbia. Larvae and pupae of the latter species differ markedly from the A. platani group. Recorded parasitoids are listed. CO1 barcodes are provided for seven species. The groups are phylogenetically not very close; the monophyly of Acalyptris and the phylogeny on the basis of CO1 (mt-DNA) sequences are discussed.

scholarly journals Effective Fundus Image Decomposition for the Detection of Red Lesions and Hard Exudates to Aid in the Diagnosis of Diabetic Retinopathy

Sensors ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 6549
Author(s):  
Roberto Romero-Oraá ◽  
María García ◽  
Javier Oraá-Pérez ◽  
María I. López-Gálvez ◽  
Roberto Hornero
Keyword(s):  
Diabetic Retinopathy ◽  
Lesion Detection ◽  
Computer Assisted ◽  
Diabetic Patients ◽  
Fundus Image ◽  
Public Database ◽  
Diagnostic Systems ◽  
Hard Exudates ◽  
The Public ◽  
Proprietary Database

Diabetic retinopathy (DR) is characterized by the presence of red lesions (RLs), such as microaneurysms and hemorrhages, and bright lesions, such as exudates (EXs). Early DR diagnosis is paramount to prevent serious sight damage. Computer-assisted diagnostic systems are based on the detection of those lesions through the analysis of fundus images. In this paper, a novel method is proposed for the automatic detection of RLs and EXs. As the main contribution, the fundus image was decomposed into various layers, including the lesion candidates, the reflective features of the retina, and the choroidal vasculature visible in tigroid retinas. We used a proprietary database containing 564 images, randomly divided into a training set and a test set, and the public database DiaretDB1 to verify the robustness of the algorithm. Lesion detection results were computed per pixel and per image. Using the proprietary database, 88.34% per-image accuracy (ACCi), 91.07% per-pixel positive predictive value (PPVp), and 85.25% per-pixel sensitivity (SEp) were reached for the detection of RLs. Using the public database, 90.16% ACCi, 96.26% PPV_p, and 84.79% SEp were obtained. As for the detection of EXs, 95.41% ACCi, 96.01% PPV_p, and 89.42% SE_p were reached with the proprietary database. Using the public database, 91.80% ACCi, 98.59% PPVp, and 91.65% SEp were obtained. The proposed method could be useful to aid in the diagnosis of DR, reducing the workload of specialists and improving the attention to diabetic patients.

The larvae of Chinese Hydropsychidae (Insecta: Trichoptera), Part I: Arctopsyche shimianensis, Parapsyche sp. A, and Diplectrona obscura

Zootaxa ◽  
2009 ◽  
Vol 2174 (1) ◽  
pp. 1-17 ◽  
Author(s):  
XIN ZHOU
Keyword(s):  
Dna Sequence ◽  
Dna Sequences ◽  
Gene Markers ◽  
Mitochondrial Coi ◽  
First Time

DNA sequence libraries (mitochondrial COI barcode and nuclear 28S D2) are built for Chinese caddisflies, a fauna largely under-studied. These independent DNA sequences are used to associate larvae and adults of Chinese Hydropsychidae. As the first part of the result of this work, Arctopsyche shimianensis Gui and Yang, 2000 and Parapsyche sp. A are associated with their adults across both gene markers. Probable association is also made for Diplectrona obscura Ulmer, 1930. Larval descriptions and illustrations are provided for all three species for the first time.

scholarly journals Taxonomic study on the photid amphipods (Senticaudata, Corophiida, Photoidea, Photidae) from Korean waters, with descriptions of a new genus and seven new species

ZooKeys ◽  
2019 ◽  
Vol 886 ◽  
pp. 1-59
Author(s):  
Tae Won Jung ◽  
Charles Oliver Coleman ◽  
Seong Myeong Yoon
Keyword(s):  
New Species ◽  
New Genus ◽  
Posterior Lobe ◽  
Closely Related Species ◽  
Korean Waters ◽  
The Family ◽  
Three New Species ◽  
First Time ◽  
A New Species ◽  
Japan And China

In this paper, seven new species of the family Photidae from Korean waters are described and illustrated in detail. Among them, Exiliphotis petilasp. nov. is a monotype of the newly reported Exiliphotisgen. nov., which is characterized by slenderer and more elongate pereopods 5–7 than those of other genera of the family Photidae. The genus Latigammaropsis is reported for the first time from Korean waters based on the description of Latigammaropsis careocavatasp. nov., which is differentiated from other Gammaropsis group by having weakly sexual dimorphic gnathopods 2, those have simple palmar margins in both sexes. Three new species of Photis are also described: Photis broncasp. nov., Photis posterolobussp. nov., and Photis longicarpussp. nov. The formerly misidentified Photis longicaudata from Japan and China as well as the Korean material could be classified as a new species, P. broncasp. nov. For that, the syntypes of P. longicaudata were re-examined, and lectotype and paralectotypes were newly designated in this study. Photis broncasp. nov. is characterized by quadrate tooth on the palmar margin medially on gnathopod 2 in both sexes. Photis posterolobussp. nov. shows a pointed posterior lobe on the ischium and a well-developed process of the propodus on male gnathopod 2. Photis longicarpussp. nov. can be distinguished from other species of the genus by very elongate carpus of male gnathopod 1. Two new species belonging to the genus Podoceropsis are also reported: Podoceropsis insinuomanussp. nov. has a strongly bisinuous palmar margin on male gnathopod 2, and Podoceropsis pseudoclavapessp. nov. differs from the closely related species of Podoceropsis clavapes by different shape of the palmar margin and shorter dactylus of male gnathopod 2. Additionally, a key to the Korean species of Photidae is provided.

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