scholarly journals Mitochondrial function and aerobic capacity assessed by high resolution respirometry in Thoroughbred horses

2016 ◽  
Vol 12 (2) ◽  
pp. 67-73 ◽  
Author(s):  
D. Serteyn ◽  
J. Ceusters ◽  
S. Nonnenmacher ◽  
K. Kirsch ◽  
A. Mouithys-Mickalad ◽  
...  

During the initial stages of training of young Thoroughbred horses, low intensity exercise is employed to increase aerobic capacity. High Resolution Respirometry (HRR) allows the determination of aerobic capacities in small samples of permeabilised muscle fibres. The aim of the study was to measure the mitochondrial function by HRR in Thoroughbred horses, to compare these values to Warmblood horses and to evaluate the effect of a 10-weeks training period. The mitochondrial function was measured by HRR using different substrate-uncoupler protocols (SUIT 1 and 2) in muscle microbiopsies from two groups of untrained horses: 17 Warmblood and 8 Thoroughbred and in the group of 8 Thoroughbred horses before and after a 10-week training period. The SUIT1 protocol employed to compare the two groups of horses showed that in Thoroughbred horses, the mean values for oxygen flux expressed as tissue mass-specific respiration were significantly higher for complex I (CI)Glutamate+Malate, CI + complex II, and maximum electron transport capacities (ETSmax) than the mean values measured in Warmblood horses. The SUIT 1 and SUIT 2 protocols revealed large differences among Thoroughbred horses before and after training. The SUIT 2 protocols showed a significant difference for the complex I activity before and after training but only when the oxygen flux was expressed as percentage of ETSmax. This study shows the interest of HRR in equine sport medicine and exercise physiology, but shows that the technique requires further refinement. Indeed significant differences have been shown between the Thoroughbred and the Warmblood horses highlighting the need to have baseline data for each breed. The Thoroughbred horses had globally a high oxidative phosphorylation capacity with an increase of CI activity induced by an aerobic training program.

2013 ◽  
Vol 24 (5) ◽  
pp. 482-486 ◽  
Author(s):  
Marili Doro Andrade Deonizio ◽  
Gilson Blitzkow Sydney ◽  
Antonio Batista ◽  
Roberto Pontarolo ◽  
Paulo Ricardo Bittencourt Guimarães ◽  
...  

This study evaluated the influence of apical patency, root filling removal technique and cleaning of the apical foramen, concerning the amount of debris extruded during root canal retreatment. Forty mandibular incisors were randomly assigned to 4 groups - GIM (n=10), GIIM (n=10), GIPT (n=10) and GIIPT (n=10), which were named according to leaving (I) or not (II) apical patency during canal preparation and filling removal technique (manual - M or ProTaper system - PT). After filling material removal, each specimen of each group had the apical foramen cleaned by sizes 15, 20 and 25 instruments, generating 12 subgroups: GIM15, GIM20, GIM25, GIIM15, GIIM20, GIIM25, GIPT15, GIPT20, GIPT25, GIIPT15, GIIPT20 and GIIPT25. Extruded filling debris was collected by a Milipore filtration system, an HV-durapore, 0.45 µm pore filter with a 25 mm diameter. The filters were weighed before and after the collection on an analytical scale (10–5 g), and the difference was calculated. The mean weight of extruded filling debris was analyzed statistically by Kruskal-Wallis and Friedman ANOVA tests (α=0.05). The mean values found in the groups (in mg) were: GIM (0.95±0.94), GIIM (0.47±0.62), GIPT (0.30±0.31) and GIIPT (0.32±0.44). There was no statistically significant difference among any of the groups or subgroups (p>0.05). ProTaper provided the smallest amount of extruded filling material, regardless of presence or absence of apical patency, followed by manual technique, without and with apical patency. Additional amounts of debris were collected during cleaning of the apical foramen, regardless of the instrument, presence/absence of patency or root filling removal technique.


2015 ◽  
Vol 11 (S317) ◽  
pp. 352-353
Author(s):  
G. Tautvaišienė ◽  
A. Drazdauskas ◽  
C. Lardo ◽  
S. L. Martell ◽  
E. Pancino ◽  
...  

AbstractWe provide CNO and Fe abundance investigations for a sample of up to 45 NGC 1851 giants. High-resolution spectra were obtained with the VLT UVES spectrograph in the framework of the Gaia-ESO Survey. The stars in our sample can be separated into two groups with a difference of 0.1 dex in the mean metallicity, 0.3 dex in the mean C/N, and no significant difference in the mean values of C+N+O.


Author(s):  
Beatriz Minghelli

AbstractBackgroundSchool physiotherapy programs can promote an increase in health literacy promoting healthier postural habits. This study aimed to verify the effectiveness of this program in improving the theoretical-practical ergonomic knowledge about postures in adolescents and to verify the postural habits adopted.MethodsThe sample comprised 206 students, 109 (52.9%) being boys, aged 12–19 years old. The measurement instruments included a theoretical-practical test and a scale. The intervention program included one session lasting 90 min, using both theoretical and practical approaches.ResultsThe mean values obtained on the theoretical-practical test before and after the 1-month period were 9.14 ± 2.51 and 13.05 ± 1.41, respectively (p ≤ 0.001). One hundred and ninety-nine (96.6%) students sat with the spine wrongly positioned, 197 (95.6%) students used mobile phones with a cervical flexion, and 165 (80.1%) lifted a heavy object from the floor incorrectly.ConclusionsThis data revealed that a school physiotherapy program improves ergonomic knowledge in adolescents.


2019 ◽  
Vol 27 (5) ◽  
pp. 270-276
Author(s):  
Erden Erol Ünlüer ◽  
Arif Karagöz ◽  
Serdar Bayata ◽  
Sibel Çatalkaya ◽  
Hüseyin Bozdemir

Background: Fluid responsiveness can be predicted by the effect of passive leg raising on cardiac output. Objectives: This research aimed to compare the changes in cardiac output and the peak systolic velocity values of Tricuspid annulus velocity at the free wall (S’) before and after passive leg raising in healthy volunteers. Methods: The study was approved by ethical commission. The desired sample size was 28, and 57 volunteers were included after they signed informed consent. The first measurements, including vital signs, S’, and cardiac output, were taken with the participants lying supine and were performed in the morning after 12 h fast. The participants were then asked to lie in a semirecumbent position for 3 min. After 3 min, the head of the bed was lowered to the supine position and the participants’ legs were elevated at 45°. Secondary measurements were repeated in this position. The differences between vital signs, cardiac output, and S’ measurements before and after passive leg raising were statistically compared. The level of significance was set as p < 0.05. Results: The mean values of cardiac output and S’ before passive leg raising was 9.59 L/min and 11.57 cm/s, respectively; however, those increased to 11.44 L/min and 13.72 cm/s after passive leg raising. The average increases were 16.17% for cardiac output and 15.67% for S’. The changes of cardiac output and S’ were statistically significant. The changes of vital signs before and after passive leg raising were statistically insignificant. Conclusion: This study has demonstrated the concordance of rise in cardiac output with S’ change by passive leg raising in healthy subjects. Further studies are needed to validate the use of S’ values in critically ill subjects.


Author(s):  
Mohammad Reza Sobhan ◽  
Seyed Hossein Saeed Banadaky ◽  
Mohammad Shafiee ◽  
Mohammad Gheisari

Introduction: Limits of movement in knees is one of the most common complications following trauma and surgery. With early diagnosis and appropriate treatment choices, knee replacement and performance improvement are expected in many patients. However, limits of movement in knees is unpleasant and should be corrected as soon as possible. In this study, we decided to evaluate the results of arthroscopic release in motion limits of knees. Methods: This was a descriptive cross-sectional study.14 patients who referred to the Shahid Sadoughi Hospital of Yazd from 2014 to 2016 and diagnosed with knee limitation (flexion or extension restriction of more than 10 degrees) were selected. Patients were undergone arthroscopic release and followed-up for 6 months. Lysholm knee score, degrees of flexion and extension, and patient satisfaction were evaluated. The results were analyzed by SPSS-18 and statistical tests Paired T-test as well as Spearman correlation. Results: The mean age of the patients was 28.92±10.99 years. The most common type of trauma in the patients was femoral distal fracture (46.7%). The frequency of the patients' satisfaction with arthroscopy was as followed: 50% (7 patients), had moderate satisfaction, 28.6% (4 ones), high satisfaction, and 21.4% (3 ones) had low satisfaction.The mean difference in Lysholm Knee Score, improvement in degree of extension, improvement in degree of flexion and pain score before and after arthroscopy were 26.21±14.11,64.78±26.13,3.57±2.92 and 4.13±1.65,respectively.There was a significant difference between the mean values of these factors before and after arthroscopy. Conclusion: According to the results obtained, we can conclude that arthroscopic release is an effective and low-complication method in motion limits of knees.


Author(s):  
Dwaaragan Subamuralitharan ◽  
BCIT School of Health Sciences, Environmental Health ◽  
Helen Heacock ◽  
Frederick Shaw

Background: Foodborne illnesses linked to fresh produce has been on the rise in recent years. This increase in illnesses pose a massive burden to the healthcare system. One way to prevent this is through educating the public the importance of handwashing produce. There are many studies that have tested ways of washing and sanitizing fresh produce to reduce surface pathogens. However the average consumer mainly hand washes produce before consuming it. Thus this study intends to test the efficacy of hand washing by testing surface ATP on apples before and after they have been washed. Methods: Apples were purchased from the bulk section of a super market. ATP swabs were used to test the concentration of ATP on the surface of the apples. The values were recorded. All the apples were hand washed under running tap water for 15 seconds and the surface ATP concentration were obtained and recorded. The values were then compared to draw a conclusion. Results: The results show that there is statistically significant reduction in surface ATP values on apples after washing them (mean ATP value of 33.2) compared to before washing them (mean ATP value of 116.67). The p-value obtained was 0.00033 when α = 0.05. Conclusion: This study was able to conclude that there was a significant reduction in surface ATP values following handwashing of the apples. There was an observed 60% reduction in the mean values of ATP of before and after hand washing. In conclusion, hand washing apples do provide an adequate reduction of surface ATP values thus attaining surface cleanliness.  


2020 ◽  
Vol 7 (06) ◽  
pp. 4848-4853
Author(s):  
Andrew Wekesa ◽  
Mazximila Wanzala

Effects of home-based convergence insufficiency vision therapy on accommodation among school going children Wekesa Andrew1, Wanzala Maximilla1 1Masinde Muliro University of Science and Technology Kakamega, Kenya.   Abstract Background: Convergence and accommodation are always yoked together and any adjustment on the former affects the later. The most effective treatment for convergence insufficiency (CI) is vision therapy. Aim/Purpose: To determine the effects of home-based vision therapy on accommodation in school-going with CI attending the Masinde Muliro University Academic Vision Center in Kenya. Methods: A clinical experimental design involving 23 participants with the mean age of 14±2.4 years, were recruited into the study, however, only 18 were assessed after the therapy. The study took 9 weeks thus majority lost contact while others moved out of the town. Home-based vision therapy which is undertaken at home using a pencil or broke strings. During the therapy, accommodative values were monitored which were; negative relative accommodation, positive relative accommodation, dynamic accommodation, accommodative facility and near point of accommodation. Paired t-test used to compare mean values before and after the therapy. Results: The mean value of negative relative accommodation (NRA) before (mention what the mean values were here) and after (mention what the mean values were here) home-based therapy was statistically significant (p=0.01). However, the mean values before and after home-based therapy for the near point of accommodation (NPA), dynamic (Lag), relativity (PRA) and facility (MAF) showed no difference (p> 0.05). The mean value of the NRA before and after office-based vision therapy was statistically significant (p=0.01). Conclusion: The use of home-based vision therapy for the patients with CI had significant effects on accommodation changing from a lower limit to average after the therapy.   Keywords: Convergence insufficiency, Accommodation, Home-based vision therapy.


2010 ◽  
Vol 108 (5) ◽  
pp. 1395-1401 ◽  
Author(s):  
H. Thomas Robertson ◽  
Melissa A. Krueger ◽  
Wayne J. E. Lamm ◽  
Robb W. Glenny

This study was designed to validate a high-resolution method to measure regional ventilation (V̇a) in small laboratory animals, and to compare regional V̇a and perfusion (Q̇) before and after methacholine-induced bronchoconstriction. A mixture of two different colors of 0.04-μm fluorescent microspheres (FMS) was aerosolized and administered to five anesthetized, mechanically ventilated rats. Those rats also received an intravenous injection of a mixture of two different colors of 15-μm FMS to measure regional blood flow (Q̇). Five additional rats were labeled with aerosol and intravenous FMS, injected with intravenous methacholine, and then relabeled with a second pair of aerosol and intravenous FMS colors. After death, the lungs were reinflated, frozen, and sequentially sliced in 16-μm intervals on an imaging cryomicrotome set to acquire signal for each of the FMS colors. The reconstructed lung images were sampled using randomly placed 3-mm radius spheres. V̇a within each sphere was estimated from the aerosol fluorescence signal, and Q̇ was estimated from the number of 15-μm FMS within each sphere. Method error ranged from 6 to 8% for Q̇ and 0.5 to 4.0% for V̇a. The mean coefficient of variation for Q̇ was 17%, and for V̇a was 34%. The administration of methacholine altered the distribution of both V̇a and Q̇ within lung regions, with a change in V̇a distribution nearly twice as large as that seen for Q̇. The methacholine-induced changes in V̇a were not associated with compensatory shifts in Q̇. Cryomicrotome images of FMS markers provide a high-resolution, anatomically specific means of measuring regional V̇a/Q̇ responses in the rat.


2018 ◽  
Vol 930 ◽  
pp. 43-47
Author(s):  
Cristiane Fonseca de Carvalho ◽  
Cláudio Luis de Melo-Silva ◽  
Tereza Cristina Favieri de Melo-Silva ◽  
Fábio Amaral de Araújo ◽  
Jefferson Fabricio Cardoso Lins

The aim of this study was to analyze the flexural strength of ceramics based on yttria-stabilized zirconia (YTZP) , used in the manufacture of dental prostheses infrastructure before and after aging with cyclic fatigue in moisture. The samples were made by pre-sintered blocks of ZirkonTransluzent (Zirkonzahn GMHB) from YTZP ceramics with and without feldspathic ceramic coating, divided into groups: G1 = YTZP control; G2 = YTZP aged; G3 = YTZP + feldspathic; G4 = YTZP + aged feldspathic. The samples were submitted to a three-point bending test with a speed of 0.5 mm / min. The mean values of the flexural strength values were G1 = 645 MPa (DP ± 124), G2 = 681 MPa (DP ± 129), G3 = 904Mpa (DP ± 157) and G4 = 954Mpa (DP ± 243). The values of groups G1 and G2; G3 and G4 were statistically similar, depending on the presence or absence of coating. Cyclic stresses did not affect the flexural strength of the material


2017 ◽  
Vol 30 (4) ◽  
pp. 789-795 ◽  
Author(s):  
Júlia Isabel de Araújo Guerra ◽  
Danilo Alves Pinto Nagem ◽  
Cristiane Aparecida Moran ◽  
Valéria Lidyanne Souza Gomes ◽  
Juliana Macedo Campelo de Carvalho ◽  
...  

Abstract Introduction: Expiratory flow increase is a maneuver of respiratory physical therapy that promotes flow direction to the upper airways however, when applied in newborns, it may result in changes of thoracoabdominal mobility. Objective: To evaluate the thoracoabdominal mobility by photogrammetry in newborns after expiratory flow increase technique. Methods: Experimental blind study performed with newborns in supine position on a support table with upper limbs flexed, abducted and externally rotated and hip flexed at 110°. Adhesive markers were allocated for geometric delimitation of the thoracoabdominal compartment and expiratory flow increase technique was performed for 5 minutes with the therapist’s hands on the thorax and abdomen. Newborns were filmed before and after the maneuver and the frames were analyzed in AutoCAD® software by a blinded investigator at the time of the procedure. The largest and the smallest thoracoabdominal area were expressed in cm2 and the mean values were compared between two moments (pre and post maneuver) by paired t test. Results: Twenty newborns with a mean age of 39 weeks were included. Before the maneuver, thoracoabdominal area was 56.1 cm2 during expiration and 59.7 cm2 during inspiration, and after the maneuver the value was 56.2 cm2 during expiration and 59.8 cm2 during inspiration, with no statistical difference between before and after (p = 0.97, p = 0.92, respectively). Conclusion: Results demonstrate that expiratory flow increase technique does not seem to change thoracoabdominal mobility of healthy newborns.


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