Isolation and Pathogenicity Determination of Bacillus cereus Associated with Ulcer Formation in African Catfish Clarias gariepinus

2020 ◽  
Vol 15 (1) ◽  
pp. 10-18
Author(s):  
Abdelgayed M. Younes ◽  
Alkhateib Y. Gaafar ◽  
Alaa Eldeen Z. ◽  
Attia A. Abou Zaid ◽  
Ahmed A. Askora
Keyword(s):  
Bacillus Cereus ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Ulcer Formation

Determination of a predictive growth model for cultivated African catfish Clarias gariepinus (Burchell, 1882)

2021 ◽  
Author(s):  
Babatunde Olaseni Musa ◽  
Alvaro Hernández‐Flores ◽  
Oludare Akanni Adeogun ◽  
Adekunle Oresegun
Keyword(s):  
Growth Model ◽  
Clarias Gariepinus ◽  
African Catfish

scholarly journals Pengaruh pemberian mikrokapsul probiotik Bacillus cereus P22 dan Staphylococcus lentus L1k pada pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele, Clarias gariepinus Burchell 1822 yang diinfeksi Aeromonas hydrophila

2018 ◽  
Vol 17 (2) ◽  
pp. 143
Author(s):  
Lilik Setiyaningsih ◽  
Widanarni Widanarni ◽  
Angela Mariana Lusiastuti3 ◽  
Munti Yuhana
Keyword(s):  
Bacillus Cereus ◽  
Growth Performance ◽  
Aeromonas Hydrophila ◽  
Clarias Gariepinus ◽  
Bacterial Count ◽  
Negative Control ◽  
Total Bacterial Count ◽  
African Catfish ◽  
Feed Conversion ◽  
Significant Difference

The aimed of this research was to evaluate the effects of dietary probiotic microcapsules B.cereus P22 and S. lentus (L1k) at different dose and frequency on growth performance, immune response and resistance of African catfish infected with A. Hydrophila. Probiotics used in this study were B. cereus P22 and S. lentus L1k encapsulated by spray draying method. The research was carried out for 56 days with eight treatments and four replications. The treatments were K- (negative control), K+ (positive control), A (feed supplemented with 0,5% of microencapsulated probiotic, fedevery day), B (feed supplemented with 0,5% of microencapsulated probiotic, fed once every three days), C (feed supplemented with 1% of microencapsulated probiotic, fed every day), D (feed supplemented with 1% of microencap-sulated probiotic with an administration once every three days), E (feed with 2% of microencapsulated probiotic with an administration every day) and F (feed with 2% of microencapsulated probiotic with an administration once every three days). On day 42, all of the fish except K- were challenged by intramuscular injection of A. hydrophila (108 CFU ml-1). In 40 days after infection, there were no significant difference on survival rate (SR) between treatments (p> 0.05). Treatment E displayed the higher growth rate (4,54±0,02%), total probiotic B. cereus (P22) and S . lentus (L1k) (4.06± 0.09 log CFU g-1; 4.02±0.08 log CFU g-1) than other treatments; whereas treatment D showed the best feed conversion rasio(1.191±0.013), and treatment F offered the highest total bacterial count (7.11±0.53 log CFU g-1). An administration of 2% microencapsulated probiotic in every day frequency demonstrated the better growth performance, immune res-ponse and resistance of African catfish to A. hydrophila compare with other treatments. AbstrakPenelitian ini bertujuan untuk mengkaji dosis dan frekuensi pemberian mikrokapsul probiotik melalui pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele yang diinfeksi Aeromonas hydrophila. Penelitian ini meng-gunakan Bacillus cereus P22 dan Staphylococcus lentus L1k yang telah dienkapsulasi melalui metode spray draying. Penelitian ini dilaksankan selama 56 hari dengan delapan perlakuan dan empat ulangan, terdiri atas perlakuan K- (kon-trol negatif), K+ (kontrol positif), A (pakan+mikrokapsul probiotik dosis 0,5% frekuensi setiap hari), B (pakan+mikro-kapsul probiotik dosis 0,5% frekuensi tiga hari sekali), C (pakan+mikrokapsul probiotik dosis 1% frekuensi setiap hari), D (pakan+mikrokapsul probiotik dosis 1% frekuensi tiga hari sekali), E (pakan+mikrokapsul probiotik dosis 2% fre-kuensi setiap hari) dan F (pakan+mikrokapsul probiotik dosis 2% frekuensi tiga hari sekali). Ikan diuji tantang dengan A. hydrophila pada hari ke 42 dengan kepadatan 108 CFU ml-1 secara intramuscular (kecuali K- diinjeksi dengan phos-phate buffer saline). Setelah 40 hari pascainjeksi, laju sintasan tidak menunjukkan perbedaan yang signifikan antarperlakuan (p>0,05).Perlakuan E menunjukkan laju pertumbuhan (4,54±0,02%) dan total probiotik B. cereus (P22) dan S. lentus (L1k) (4,06±0,09 log CFU g-1; 4,02±0,08 log CFU g-1) tertinggi; sementara perlakuan D memberikan hasil nisbah konversi pakan terbaik (1,191±0,013), perlakuan F menunjukkan total bacterial count tertinggi (7,11±0,53 log CFU g-1). Hasil penelitian menunjukkan bahwa dosis 2% yang diberikan setiap hari memberikan hasil yang lebih baik dalam meningkatkan laju pertumbuhan, respons imun, dan resistensi ikan lele terhadap A. hydrophila. 

scholarly journals Morphometric and histological features of the testicles of cultured male broodstock African Catfish (Clarias gariepinus) at different ages

2018 ◽  
Vol 68 (2) ◽  
pp. 211 ◽  
Author(s):  
OKOYE CHIDOZIE NWABUISI ◽  
ANIETIE FRANCIS UDOUMOH ◽  
DAN-JUMBO SUSAN OMUBOBA ◽  
EZE UKAMAKA UCHENNA ◽  
OZOKOYE ANTHONY CHUKWUEMEKA ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
Fish Population ◽  
Seminiferous Tubules ◽  
African Catfish ◽  
Spermatogenic Cells ◽  
Morphological Adaptations ◽  
The Mean ◽  
Partial Distortion ◽  
Apparently Healthy

Testicular tissues obtained from 25 apparently healthy broodstock African Catfish (Clarias gariepinus) of 6, 8, 9, 10 and 12 months old were used to investigate the morphological adaptations of the testicles suitable for reproductive exercises. The result showed that the mean gonadosomatic indices of the pooled testicles of the catfish increased linearly from 0.30 ± 0.02, 0.34 ± 0.01, 0.45 ± 0.09 at 6, 8, and 9 months of age respectively and peaked at 12 months with a GSI value of 0.61±0.13. Moreover, the epithelial height of the seminiferous tubules of 9 month old catfish was significantly (P<0.05) higher than the epithelial heights of the 6, 8, 10 and 12 month old fish. The luminal diameters of the seminiferous tubules of the testicles of 10 and 12 months old catfish were significantly (P<0.05) higher than the luminal diameters of the 6, 8 and 9 months old fish. The encapsulated testicles of the catfish exhibited seminiferous lobules which contained spermatogenic cells in cysts. However, at 10 months, there were partial distortion of the cystic distribution of the spermatogenic cells and occasional loss of the germinal epithelium while evidences of necrosis of some of the cells of the seminiferous epithelium were recorded in the testicles of 12 months old fish. Finally, the present study has provided morphological evidences that the use of gonadosomatic indices alone in the determination of the reproductive state of a fish population may give an erroneous picture and that the 8 and 9 months old broodstock African Catfish (Clarias gariepinus) may be most suitable for breeding exercises.

Seroconversion, seroprotection and safety determination of formalinised local isolate of Aeromonas hydrophila administered through different routes in African catfish (Clarias gariepinus, Burchell 1822)

2019 ◽  
Vol 43 (3) ◽  
pp. 79-87
Author(s):  
Saka Babatunde Akeem ◽  
Adeyemo Olanike Kudirat ◽  
Odeniyi Olubusola Ayoola ◽  
Alarape Selim Adewale ◽  
Odeseye Adebola Olayemi
Keyword(s):  
Aeromonas Hydrophila ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Local Isolate

Determination of quarantine period in African catfish (Clarias gariepinus) fed with pig (Sus sp.) offal to assure compliance with halal standards

2012 ◽  
Vol 135 (3) ◽  
pp. 1268-1272 ◽  
Author(s):  
M.N. Wan Norhana ◽  
G.A. Dykes ◽  
B. Padilah ◽  
A.A. Ahmad Hazizi ◽  
A.R. Masazurah
Keyword(s):  
Clarias Gariepinus ◽  
African Catfish

Thermosensitivity of the sex differentiation process in the African catfish, Clarias gariepinus: Determination of the thermosensitive period

Aquaculture ◽  
2016 ◽  
Vol 455 ◽  
pp. 73-80 ◽  
Author(s):  
Saïdou Santi ◽  
Vincent Gennotte ◽  
Aboubacar Toguyeni ◽  
Charles Mélard ◽  
Nadine Antoine ◽  
...  
Keyword(s):  
Sex Differentiation ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Differentiation Process ◽  
Thermosensitive Period

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

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