scholarly journals Determination of Digestive Enzyme Activity in the Digesta from the Small Intestinal of Growing Pigs and Development of in vitro Evaluation System for Feed Bioavailability Using Artificial Small Intestinal Juice

2012 ◽  
Vol 11 (11) ◽  
pp. 1819-1826 ◽  
Author(s):  
Defa Li ◽  
Luoyun Fang ◽  
Hongfu Zhang ◽  
Feng Zhao ◽  
Junjun Wang
Keyword(s):  
Enzyme Activity ◽  
Evaluation System ◽  
Digestive Enzyme ◽  
Growing Pigs ◽  
Digestive Enzyme Activity ◽  
In Vitro Evaluation ◽  
Intestinal Juice ◽  
Small Intestinal

scholarly journals Effects of dietary crude protein level and N-carbamylglutamate supplementation on nutrient digestibility and digestive enzyme activity of jejunum in growing pigs

2020 ◽  
Vol 98 (4) ◽  
Author(s):  
Yuming Wang ◽  
Shuaijuan Han ◽  
Junyan Zhou ◽  
Peili Li ◽  
Gang Wang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Crude Protein ◽  
Digestive Enzyme ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Digestive Enzyme Activity ◽  
Adaptation Period ◽  
Ileal Digestibility ◽  
Dietary Crude Protein ◽  
Dietary Treatments

Abstract Three experiments were conducted to investigate the effects of dietary crude protein (CP) level and N-carbamylglutamate (NCG) supplementation on apparent total tract digestibility (ATTD) and ileal digestibility of nutrients and digestive enzyme activity of jejunum in growing pigs. In experiment 1, 10 Duroc × Landrace × Yorkshire barrows (initial BW: 48.7 kg) were allotted to a three-period switchback design with five experimental diets and two replicate pigs per diet in each period. Diets were categorized as high CP (HP, 18% CP), moderate low CP (MLP, 15% CP), very low CP (VLP, 12% CP), and MLP and VLP with 0.1% NCG supplementation. Feces and urine were collected from day 6 to day 11 after a 5-d adaptation period. The DE, ME, and ATTD of GE, OM, CP, NDF, ADF, and P decreased (P < 0.01) with a reduction of dietary CP, but no effect of dietary treatments on pig daily N retention was detected. The NCG supplementation increased (P < 0.01) DE and ATTD of ADF of the VLP diet. In experiment 2, 10 jejunal-cannulated Duroc × Landrace × Yorkshire barrows (initial BW: 44.5 kg) were fed five diets for three periods as experiment 1. Jejunal fluid was collected on days 6 and 8 after a 5-d adaptation period. The digestive enzymes activity was not affected by dietary CP level, except for α-amylase, for which there was a decrease (P < 0.01) in pigs fed VLP diets compared to HP and MLP diets. In experiment 3, 12 ileal-cannulated Duroc × Landrace × Yorkshire barrows (initial BW: 46.7 kg) were allotted to a three-period switchback design with six diets and two replicate pigs per diet in each period. The six experimental diets consisted of five experimental diets as experiment 1 and one N-free diet. Ileal digesta was collected from day 6 to day 8 after a 5-d adaptation period. Results indicated that apparent ileal digestibility (AID) of CP and P and ileal digestibility of Arg, His, Ile, Leu, Phe, and all dispensable AA, except Pro, decreased (P < 0.01) in pigs fed VLP diet compared to HP and MLP diets, but AID of GE, OM, EE, NDF, and ADF were not affected. The supplementation of NCG in the VLP diet increased (P < 0.01) the AID of CP and ileal digestibility of Arg, His, Leu, Phe, Val, Ser, and Tyr. In conclusion, reducing dietary CP level decreased nutrient digestibility, but improved the efficiency of dietary N utilization and reduced N emission. Moderate reduction of dietary CP level had a minimal effect on nutrient digestibility and digestive enzyme activity. Additionally, NCG supplementation plays a beneficial effect on nutrient digestion only if the dietary CP level is extremely lowered.

Changes in Small Intestinal Morphology and Digestive Enzyme Activity with Oral Administration of Copper-Loaded Chitosan Nanoparticles in Rats

2011 ◽  
Vol 145 (3) ◽  
pp. 355-360 ◽  
Author(s):  
Xin-Yan Han ◽  
Wen-Li Du ◽  
Qi-Chun Huang ◽  
Zi-Rong Xu ◽  
Yi-Zheng Wang
Keyword(s):  
Enzyme Activity ◽  
Oral Administration ◽  
Digestive Enzyme ◽  
Chitosan Nanoparticles ◽  
Intestinal Morphology ◽  
Digestive Enzyme Activity ◽  
Small Intestinal

Some Sources of Variability in Determination of Digestive Enzyme Activity in Zooplankton

1980 ◽  
Vol 37 (9) ◽  
pp. 1426-1432 ◽  
Author(s):  
P. Mayzaud
Keyword(s):  
Enzyme Activity ◽  
Physiological Activity ◽  
Digestive Enzyme ◽  
Protein Solubility ◽  
Digestive Enzyme Activity ◽  
Acetone Powder ◽  
Plankton Sample ◽  
Marine Zooplankton ◽  
Activity Measurements

Enzyme activity measurements, sometimes used to ascertain the distribution and quantification of physiological activity of marine zooplankton, are sensitive to a number of sources of variability of both methodological and environmental origins. To facilitate multiple enzyme determinations on a single sample, we have developed a method of acetone extraction from neritic zooplankton followed by 50% glycerol homogenization of the resulting powder. An increase in activity was found compared with that of glycerol homogenates of live zooplankton. Because saturation of 50% glycerol was found when extracting increasing amounts of acetone powder, care was taken to prepare homogenates with an identical amount of powder. The problem of protein solubility in the homogenization medium is discussed. The method is repeatable and yields results which are little influenced by short-term storage of animals before extraction but are strongly influenced by species composition of the plankton sample and to a lesser extent by spatial variability of the plankton populations.Key words: variability, determination, digestive enzyme activity, zooplankton

Changes in Small Intestinal Digestive Enzyme Activity and Bile Acids with Dietary Cellulose in Rats

1980 ◽  
Vol 110 (3) ◽  
pp. 584-590 ◽  
Author(s):  
Barbara O. Schneeman ◽  
Daniel Gallaher
Keyword(s):  
Enzyme Activity ◽  
Bile Acids ◽  
Digestive Enzyme ◽  
Digestive Enzyme Activity ◽  
Small Intestinal

Effects of Bacillus subtilis on growth, digestive enzyme activity, and non-specific immunity in hybrid sturgeon (Acipenser baeri ♂×Acipenser schrenkii ♀) juveniles

2013 ◽  
Vol 18 (6) ◽  
pp. 1315-1320 ◽  
Author(s):  
Xiaoyong LIU ◽  
Ying ZHANG ◽  
Qian QI ◽  
Mingjun ZHAO ◽  
Likai MAI ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Digestive Enzyme ◽  
Digestive Enzyme Activity ◽  
Acipenser Baeri ◽  
Specific Immunity ◽  
Hybrid Sturgeon
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