Identification of Bacterial Species with Nitrogen, Phosphorus and Sulfur Bioremediation Pathways in Wastewater Treatment Plants

2020 ◽  
Vol 14 (1) ◽  
pp. 1-12
Author(s):  
Laura Rodrigues Araú ◽  
Aulus Estevão Anjos de D
2019 ◽  
Vol 80 (12) ◽  
pp. 2352-2361
Author(s):  
L. M. L. Silva ◽  
A. F. Santiago ◽  
G. A. Silva ◽  
A. L. P. Castro ◽  
L. S. Bastos ◽  
...  

Abstract The use of light-emitting diode (LED)-illuminated photobioreactors with microalgae has been extensively studied for wastewater treatment. Most studies have used isolated microalgae species; however, this practice does not match the reality of conditions in wastewater treatment plants. Operational conditions that promote greater growth of algal biomass and that remove pollutants most effectively are disputed in the literature. In this context, LED-illuminated photobioreactors with microalgae were evaluated using multivariate analysis in order to optimize removal of pollutants (nitrogen, phosphorus, and carbonaceous organic matter). Three variables were evaluated: operating time, LED wavelength, and luminous flux intensity. A microalgae consortium was used in the photobioreactor. In addition to the LED-illuminated photobioreactors, control photobioreactors illuminated by sunlight were also operated. Using the results obtained in the optimization, a scaled-up reactor approximately 8.5 times larger in volume was operated to evaluate if the behavior would be maintained. The best operational conditions for the removal of pollutants were observed in LED-illuminated photobioreactors operated under a light intensity of 700 μmol·m−2s−1 for 15 days. Under these conditions, it was possible to remove 89.97% of carbonaceous organic matter, 86.50% of nitrogen, and 30.64% of phosphorus. The scaled-up photobioreactor operated with similar performance.


Microbiology ◽  
2006 ◽  
Vol 152 (10) ◽  
pp. 3003-3012 ◽  
Author(s):  
Caroline Kragelund ◽  
Yunhong Kong ◽  
Jaap van der Waarde ◽  
Karin Thelen ◽  
Dick Eikelboom ◽  
...  

The ecophysiology of five filamentous species affiliated to the Alphaproteobacteria was investigated in industrial activated sludge systems. The five species, ‘Candidatus Alysiosphaera europaea’, ‘Candidatus Monilibacter batavus’, ‘Candidatus Alysiomicrobium bavaricum’, ‘Candidatus Sphaeronema italicum’ and Meganema perideroedes, are very abundant in industrial wastewater treatment plants and are often involved in bulking incidents. The morphology of these filamentous bacterial species resembled Eikelboom's Nostocoida limicola, or Type 021N, and could only be correctly identified by using fluorescence in situ hybridization (FISH), applying species-specific gene probes. Two physiological groupings of the five species were found using microautoradiography combined with FISH. Group 1 (‘Ca. Monilibacter batavus' and ‘Ca. Sphaeronema italicum’) utilized many short-chained fatty acids (acetate, pyruvate and propionate), whereas Group 2 (‘Ca. Alysiosphaera europaea’, ‘Ca. Alysiomicrobium bavaricum’ and Meganema perideroedes) could also exploit several sugars, amino acids and ethanol. All species had polyhydroxyalkanoate granules present and several of the species had a very large storage capacity. No activity was found under strict anaerobic conditions, while uptake of substrate was observed in the presence of nitrate or nitrite as potential electron acceptor. However, for all species a reduced number of substrates could be consumed under these conditions compared to aerobic conditions. Only a little exo-enzymic activity was found and nearly all species had a hydrophobic cell surface. Based on knowledge of the ecophysiological potential, control strategies are suggested.


PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0244443
Author(s):  
John J. Kelly ◽  
Maxwell G. London ◽  
Amanda R. McCormick ◽  
Miguel Rojas ◽  
John W. Scott ◽  
...  

Microplastics are ubiquitous contaminants in aquatic habitats globally, and wastewater treatment plants (WWTPs) are point sources of microplastics. Within aquatic habitats microplastics are colonized by microbial biofilms, which can include pathogenic taxa and taxa associated with plastic breakdown. Microplastics enter WWTPs in sewage and exit in sludge or effluent, but the role that WWTPs play in establishing or modifying microplastic bacterial assemblages is unknown. We analyzed microplastics and associated biofilms in raw sewage, effluent water, and sludge from two WWTPs. Both plants retained >99% of influent microplastics in sludge, and sludge microplastics showed higher bacterial species richness and higher abundance of taxa associated with bioflocculation (e.g. Xanthomonas) than influent microplastics, suggesting that colonization of microplastics within the WWTP may play a role in retention. Microplastics in WWTP effluent included significantly lower abundances of some potentially pathogenic bacterial taxa (e.g. Campylobacteraceae) compared to influent microplastics; however, other potentially pathogenic taxa (e.g. Acinetobacter) remained abundant on effluent microplastics, and several taxa linked to plastic breakdown (e.g. Klebsiella, Pseudomonas, and Sphingomonas) were significantly more abundant on effluent compared to influent microplastics. These results indicate that diverse bacterial assemblages colonize microplastics within sewage and that WWTPs can play a significant role in modifying the microplastic-associated assemblages, which may affect the fate of microplastics within the WWTPs and the environment.


2001 ◽  
Vol 44 (6) ◽  
pp. 47-54 ◽  
Author(s):  
M. Cotman ◽  
J. Zagorc-Koncan ◽  
A. Droic

Surface waters are used for disposal of treated effluents from wastewater treatment plants. These effluents usually contain only small amounts of various contaminants but these harmful components accumulate over time in the river, especially in sediments. An integrated approach for the evaluation of the impact of treated effluents was used to predict an ecological risk assessment to the Krka river beside Novo Mesto. The effluents from pharmaceutical and municipal wastewater treatment plants were discharged too closely into the receiving stream, so that separate impacts could not be distinguished. Biologically treated industrial effluents contained great amounts of barely biodegradable organic pollution, organic nitrogen, ammonia and phosphorus, and sometimes zinc. The toxicity of effluents was mostly dependent on their chemical composition. The municipal discharge contained greater amounts of organic pollution that was completely biodegraded but still had a great nutrient pollution load. The effluents were nontoxic. The harmful substances from the effluents were traced in the river. In the downstream site slightly higher concentration of organic pollutants, organic nitrogen, phosphorus, and zinc were detected due to discharges. The river water was over-saturated with oxygen, especially in the summer. In toxicity tests, samples of the river water were nontoxic. Sediments at the downstream site accumulated from discharged organic nitrogen, phosphorus, or zinc. The results of our study show that the main problem is eutrophication of the river Krka, so it is obvious we must reduce the quantity of nutrients in the effluents from wastewater treatment plants. In both effluents it will be necessary to reduce the polluting load of phosphorus, the limiting element for growth of algae and macrophytes in the receiving stream. In the industrial effluent it will be necessary to reduce substances which cause toxicity, such as zinc and nonbiodegradable organic compounds that may be accumulated in the water ecosystem over time.


2020 ◽  
Vol 15 (2) ◽  
pp. 142-151
Author(s):  
Peter Lukac ◽  
Lubos Jurik

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.


2010 ◽  
Vol 9 (9) ◽  
pp. 1251-1258 ◽  
Author(s):  
George Barjoveanu ◽  
Claudia Cojocariu ◽  
Brindusa Robu ◽  
Carmen Teodosiu

2016 ◽  
Vol 15 (9) ◽  
pp. 1981-1988 ◽  
Author(s):  
Carmine Fiorentino ◽  
Maurizio Luca Mancini ◽  
Luca Luccarini

2013 ◽  
Vol 12 (1) ◽  
pp. 163-166 ◽  
Author(s):  
Mariana Turcu ◽  
Valentin Nedeff ◽  
Narcis Barsan ◽  
Emilian Florin Mosnegutu ◽  
Mirela Panainte

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