Yeasts and filamentous fungi associated with smooth (non-lenticel) and lenticel bark of young and scaffold branches of peach (Prunus persica (L.) Batsch) were monitored using bark washing and direct or impression plating techniques and scanning electron microscopy during potential Cystospora canker infection periods. Total populations of fungi were high in the fall but dropped in the winter and increased during the spring. Yeasts and yeast-like fungi predominated in the spring and fall samples. The principal yeasts were Basidiomycetes in the form genera Cryptococcus, Rhodotorula, and Sporobolomyces. The yeast-like fungi were Aureobasidium and Taphrina. The principal filamentous fungi were in the form genera Alternaria, Epicoccum, Cladosporium, Coniothyrium, and Libertella. The canker pathogens, Leucostoma persoonii (Nits.) Höhn. and Leucostoma cincta (Pers. & Fr.) Höhn., were observed mainly in the spring sampling. Lenticels supported greater fungal populations than smooth (non-lenticel) bark surfaces. The impact of fungal epiphytes, particularly the yeasts, on the potential biological control of peach canker is discussed.Key words: Cytospora canker, mycoflora, yeast, bark, biological control, fungal epiphyte.