scholarly journals Seed Viability and Symbiotic Seed Germination in Vanilla spp. (Orchidaceae)

2017 ◽  
Vol 10 (2) ◽  
pp. 43-52 ◽  
Author(s):  
Y.A. Alomia ◽  
A.T. Mosquera-E ◽  
N.S. Flanagan ◽  
J.T. Otero
Keyword(s):  
Seed Germination ◽  
Seed Viability ◽  
Symbiotic Seed Germination

scholarly journals Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

2021 ◽  
Author(s):  
Filippo Guzzon ◽  
Maraeva Gianella ◽  
Jose Alejandro Velazquez Juarez ◽  
Cesar Sanchez Cano ◽  
Denise E Costich
Keyword(s):  
Seed Germination ◽  
Plant Genetic Resources ◽  
Seed Viability ◽  
Storage Conditions ◽  
Seed Longevity ◽  
Germplasm Bank ◽  
Significant Difference ◽  
Different Temperatures ◽  
Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

scholarly journals Transcriptomic analyses reveal clathrin-mediated endocytosis involved in symbiotic seed germination of Gastrodia elata

2017 ◽  
Vol 58 (1) ◽  
Author(s):  
Xu Zeng ◽  
Yuanyuan Li ◽  
Hong Ling ◽  
Sisi Liu ◽  
Mengmeng Liu ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gastrodia Elata ◽  
Symbiotic Seed Germination

scholarly journals Using Microwave Soil Heating to Inhibit Invasive Species Seed Germination

2017 ◽  
Vol 10 (3) ◽  
pp. 262-270 ◽  
Author(s):  
Mélissa De Wilde ◽  
Elise Buisson ◽  
Nicole Yavercovski ◽  
Loïc Willm ◽  
Livia Bieder ◽  
...  
Keyword(s):  
Invasive Species ◽  
Soil Moisture ◽  
Seed Germination ◽  
Invasive Plant ◽  
Organic Matter Content ◽  
Seed Viability ◽  
Germination Percentage ◽  
Matter Content ◽  
Burial Depth ◽  
Experimental Conditions

Successful invasive plant eradication is rare, because the methods used target the adult stage, not taking into account the development capacity of a large seedbank. Heating by microwave was considered, because it offers a means to quickly reach the temperature required for loss of seed viability and inhibition of germination. Previous results were not encouraging, because homogeneous and deep-wave penetration was not achieved, and the various parameters that can affect treatment effectiveness were incompletely addressed. This study aimed to determine, under experimental conditions, the best microwave treatment to inhibit invasive species seed germination in terms of power (2, 4, 6 kW) and duration (2, 4, 8 min) of treatments and depending on soil moisture (10%, 13%, 20%, 30%) and seed burial depth (2, 12 cm). Three invasive species were tested: Bohemian knotweed, giant goldenrod, and jimsonweed. The most effective treatments required relatively high power and duration (2kW8min, 4kW4min, 6kW2min, and 6kW4min; 4kW8min and 6kW8min were not tested for technical reasons), and their effectiveness diminished with increasing soil moisture with germination percentage between 0% and 2% for the lowest soil moisture, 0% and 56% for intermediate soil moisture, and 27% and 68% in control treatments. For the highest soil moisture, only 2kW8min and 4kW4min reduced germination percentage between 2% and 19%. Occasionally, germination of seeds located at the 12-cm depth was more strongly affected. Giant goldenrod seeds were the most sensitive, probably due to their small size. Results are promising and justify further experiments before developing a field microwave device to treat large volumes of soil infested by invasive seed efficiently and with reasonable energy requirements. Other types of soil, in terms of texture and organic matter content, should be tested in future experiments, because these factors influence soil water content and, consequently, microwave heating.

scholarly journals Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 490
Author(s):  
Saeng Geul Baek ◽  
Jin Hyun Im ◽  
Myeong Ja Kwak ◽  
Cho Hee Park ◽  
Mi Hyun Lee ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Seed Viability ◽  
Cold Stratification ◽  
Dormancy Breaking ◽  
Viability Test ◽  
Physiological Dormancy ◽  
Different Temperatures ◽  
Germination Requirements

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

scholarly journals Flowering, Fecundity, Seed Germination, and Seed Viability of Viburnum opulus L. Cultivars

2009 ◽  
Vol 27 (1) ◽  
pp. 31-36
Author(s):  
Janine R. Conklin ◽  
James C. Sellmer
Keyword(s):  
Seed Germination ◽  
Seed Production ◽  
Seed Viability ◽  
Biological Traits ◽  
Limited Information ◽  
Viable Seed ◽  
Invasive Potential ◽  
Short Term ◽  
Viburnum Opulus ◽  
Tetrazolium Test

Abstract Mature specimens of Viburnum opulus and cultivars ‘Leonard's Dwarf’ and ‘Roseum’ were assessed over 2 years for flower and seed production, seed germination, and seed viability as determined by a tetrazolium test to understand their invasive potential. ‘Aureum’, ‘Compactum’, ‘Losely's Compact’, ‘Nanum’, and ‘Xanthocarpum’ were also tested for germination and viability of seeds. Cultivars differed in flower and seed production, seed germination, and seed viability. ‘Roseum’ prolifically produced highly viable seed that germinated at moderate rates under greenhouse conditions (8,354, 100%, and 73%, respectively). Viburnum opulus and ‘Leonard's Dwarf’ produced fewer viable seed which showed moderate to low germination rates (609, 100%, and 53%; 712, 100%, and 5%, respectively). ‘Aureum’ and ‘Xanthocarpum’ seeds germinated at moderate rates (55 and 25%, respectively) and were highly viable (100%). ‘Compactum’, ‘Losely's Compact’, and ‘Nanum’ germinated at low rates or failed to germinate (0, 0, and 5%, respectively), yet seeds were moderately viable (37, 65, and 55%, respectively). Seeds of all cultivars germinated at low rates or failed to germinate at both outdoor sites (0 to 5%) which suggests these plants may be weakly invasive. Short-term studies on biological traits such as these provide only limited information to assess the invasive potential of cultivars.

Indopiptadenia Oudhensis (Brandis) Brenan an Endangered Tree Species: Measures for the Coservation

2014 ◽  
Vol 37 (1) ◽  
pp. 69-72
Author(s):  
Giriraj Panwar ◽  
Kumar Ambrish ◽  
S. Srivastava
Keyword(s):  
Seed Germination ◽  
Tree Species ◽  
Seed Viability ◽  
Habitat Destruction ◽  
Anthropogenic Pressure ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Poor Seed ◽  
Over Exploitation

Indopiptadenia oudhensis (Brandis) Brenan is an endangered tree species of family Mimosaceae. Species is mainly distributed at Indo-Nepal border and facing threats such as anthropogenic pressure, habitat destruction, over exploitation, low seed viability and poor seed germination.

scholarly journals Seed germination at different stratification temperatures and development of Phytelephas macrocarpa Ruiz & Pavón seedlings

2017 ◽  
Vol 39 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Sidney Alberto do Nascimento Ferreira ◽  
Daniel Felipe de Oliveira Gentil
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Seed Viability ◽  
Seedling Development ◽  
Development Stages ◽  
Plastic Bags ◽  
Alternating Temperature

Abstract: Phytelephas macrocarpa (ivory palm) is an Amazonian palm vulnerable to exploitation pressure, as its seeds are widely used in regional handicrafts. The aims of this study were to evaluate the effectiveness of different stratification temperatures in overcoming seed dormancy and to analyze the seedling development stages of this species. In germination under stratification, the seeds were placed in plastic bags containing moistened vermiculite, and maintained at the constant temperatures of 25, 30, 35 and 40 °C, and an alternating temperature from 26 to 40 °C. In the study about the development of seedlings, seeds were sown in vermiculite under plastic cover (growing house), and the evolution of the seedling was evaluated, from the formation of germinative button to the complete blade expansion of the first eophyll. Stratification at alternating temperatures (26 to 40 °C) helped overcoming seed dormancy. Stratification at 25 °C kept the seed viability for nine months. Germination, characterized by the formation of the germinative button took an average of 114 ± 24 days, and the seedling development until the first extended eophyll lasted 244 ± 57 days.

Mycofloral Succession and Viability Losses in Pigeon pea Seed in Puerto Rico

1969 ◽  
Vol 68 (1) ◽  
pp. 19-31
Author(s):  
P. R. Hepperly ◽  
R. Rodríguez
Keyword(s):  
Puerto Rico ◽  
Soil Moisture ◽  
Seed Germination ◽  
Seed Viability ◽  
Pigeon Pea ◽  
Fungal Colonization ◽  
Fusarium Spp ◽  
Storage Losses ◽  
Highly Correlated ◽  
Total Fungi

Using the cellulose pad and potato dextrose agar (PDA) assays, twenty three fungi were found on pigeonpea seed in Puerto Rico. In the PDA assay, Botryodiplodia theobromae was the most common (29%). On cellulose pads, the same fungus was not very common (7%). PDA assay favored increased detection of Alternaria tenuissima, Phomopsis sp., and total fungi compared with cellulose pads. Cellulose pads favored detection of Cladosporium sp. In both assays, incidences of B. theobromae, Fusarium spp., and total fungi were negatively correlated with seed germination. Measurements for seedlot germination were highly correlated (r = 0.77**) between the two assays. The influences of seed type and delayed harvest on pigeonpea seed viability were studied. Pigeonpeas "28-Bushy," with large tan seeds, showed reduced seed viability and higher incidence of B. theobromae and Fusarium spp. compared with segregants of the same cultivar with small flattened hard red seed. A 3-week delayed harvest drastically increased incidence of B. theobromae, Fusarium spp., and total fungi, and reduced germination in "Kaki" pigeonpeas from Santa Isabel. Pigeonpea seed viability and mycoflora were followed before and after farm storage. Incidences of Fusarium spp., Phomopsis sp., Cladosporium sp., and B. theobromae after storage were 15, 26, 30, and 38% of their respective prestorage values. Incidence of species of Penicillium, Rhizopus, and Aspergillus increased during storage. Although less than 3% Aspergillus spp. was detected in the cellulose pad assay at 27° C, 28 to 92% was detected when seed were incubated at 35° C using pigeonpea seed extract in 2% agar. With the Aspergillus selective assay, seed viability losses during storage were highly correlated with incidence of Aspergillus spp. (r = 0.96**). Aspergillus incidence in the cellulose pad assay was not associated with storage losses in seed germination (r = 0.18 NS). Emergence and fungal colonization of pigeonpea seed were dependent on soil moisture. Pigeonpea seed did not emerge at either 25 or 100% soil moisture holding capacity (SMHC). Optimum emergence was found at 50% SMHC. Pythiaceous fungi were predominent on seed at and above 75% SMHC, whereas Aspergillus spp. predominated at 50% SMHC or less. Mixed populations of the two were visible at 50 and 75% SMHC.

Seed and Germination Biology ofDittrichia graveolens(Stinkwort)

2013 ◽  
Vol 6 (3) ◽  
pp. 371-380 ◽  
Author(s):  
Rachel N. Brownsey ◽  
Guy B. Kyser ◽  
Joseph M. DiTomaso
Keyword(s):  
Seed Germination ◽  
Seedling Emergence ◽  
Management Strategies ◽  
Seed Viability ◽  
Base Temperature ◽  
Time Model ◽  
Wide Range ◽  
Total Seed ◽  
Thermal Time Model ◽  
Seed Characteristics

AbstractUnderstanding seed characteristics and seedling establishment patterns is essential for the development of effective management strategies for invasive annual species.Dittrichia graveolens(stinkwort) has increased its range rapidly within California since 1995, yet its biology is not well understood, which has led to poorly timed management. In this study, seed viability, germination, longevity, and dormancy, as well as seedling emergence characteristics ofD. graveolenswere evaluated in field, greenhouse, and laboratory experiments in Davis, CA, over a 2-yr period (fall 2010 to summer 2012). In the laboratory, seed germination ofD. graveolensoccurred at a wide range of constant temperatures (12 to 34 C). Cumulative germination was comparable to total seed viability (80 to 95%) at optimal germination temperatures, indicating that primary (innate) dormancy is likely absent. The base temperature for germination was identified using a thermal time model: 6.5 C and 4 C for 2010 and 2011 seed populations, respectively. In the field, seedlings emerged from fall through spring following precipitation events. A very low percentage of seedlings (2.5%) emerged in the second year after planting. Equivalent seedling emergence was observed over a wide range of light conditions (100, 50, 27, and 9% of available sunlight) in a greenhouse experiment, indicating that seed germination is not limited by high or low light. Results from these seed experiments improve our understanding of the reproductive biology of this rapidly expanding exotic annual and provide valuable information for developing effective timing and longevity of management programs.

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