Flowering, Fecundity, Seed Germination, and Seed Viability of Viburnum opulus L. Cultivars

Abstract Mature specimens of Viburnum opulus and cultivars ‘Leonard's Dwarf’ and ‘Roseum’ were assessed over 2 years for flower and seed production, seed germination, and seed viability as determined by a tetrazolium test to understand their invasive potential. ‘Aureum’, ‘Compactum’, ‘Losely's Compact’, ‘Nanum’, and ‘Xanthocarpum’ were also tested for germination and viability of seeds. Cultivars differed in flower and seed production, seed germination, and seed viability. ‘Roseum’ prolifically produced highly viable seed that germinated at moderate rates under greenhouse conditions (8,354, 100%, and 73%, respectively). Viburnum opulus and ‘Leonard's Dwarf’ produced fewer viable seed which showed moderate to low germination rates (609, 100%, and 53%; 712, 100%, and 5%, respectively). ‘Aureum’ and ‘Xanthocarpum’ seeds germinated at moderate rates (55 and 25%, respectively) and were highly viable (100%). ‘Compactum’, ‘Losely's Compact’, and ‘Nanum’ germinated at low rates or failed to germinate (0, 0, and 5%, respectively), yet seeds were moderately viable (37, 65, and 55%, respectively). Seeds of all cultivars germinated at low rates or failed to germinate at both outdoor sites (0 to 5%) which suggests these plants may be weakly invasive. Short-term studies on biological traits such as these provide only limited information to assess the invasive potential of cultivars.

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Germination and Seed Viability of Norway Maple Cultivars, Hybrids, and Species

HortTechnology ◽

10.21273/hortsci.19.1.120 ◽

2009 ◽

Vol 19 (1) ◽

pp. 120-126 ◽

Cited By ~ 2

Author(s):

Janine R. Conklin ◽

James C. Sellmer

Keyword(s):

Seed Viability ◽

Acer Platanoides ◽

Limited Information ◽

Invasive Potential ◽

Short Term ◽

Initial Attempt ◽

Tetrazolium Chloride ◽

Testing Procedures ◽

Viability Testing ◽

Norway Maple

Norway maple (Acer platanoides), 15 cultivars, and two hybrids were assessed for 3 years to evaluate seed germination (e.g., growth chamber, open landscape, and forest) and viability in an initial attempt to understand their invasive potential. Differences in germination were observed among the cultivars over the study. Low to moderate germination was found in growth chambers (0%–35%). Lower germination was found at open landscape (0%–23%) and forest floor sites (0%–18%). Inconsistent viability was observed among cultivars across the seed lots. Viability testing with tetrazolium chloride under two protocols revealed that seed viability was higher than germination and that viability alone was not a good estimate of germination potential. Differences in the accuracy of viability testing procedures were also observed. Short-term germination and viability studies provide only limited information to characterize the possible invasiveness of cultivars.

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Do applications of systemic herbicides when green fruit are present prevent seed production or viability of Alliaria petiolata (garlic mustard)?

Invasive Plant Science and Management ◽

10.1017/inp.2021.8 ◽

2021 ◽

pp. 1-17

Author(s):

Leo Roth ◽

José Luiz C. S. Dias ◽

Christopher Evans ◽

Kevin Rohling ◽

Mark Renz

Keyword(s):

Seed Production ◽

Seed Viability ◽

Early Spring ◽

Alliaria Petiolata ◽

Late Spring ◽

Garlic Mustard ◽

Viable Seed ◽

Application Timing ◽

Control Seed ◽

Second Year

Garlic mustard [Alliaria petiolata (M. Bieb.) Cavara & Grande] is a biennial invasive plant commonly found in the northeastern and midwestern United States. Although it is not recommended to apply herbicides after flowering, land managers frequently desire to conduct management during this timing. We applied glyphosate and triclopyr (3% v/v and 1% v/v using 31.8% and 39.8% acid equivalent formulations, respectively) postemergence to established, second-year A. petiolata populations at three locations when petals were dehiscing, and evaluated control, seed production and seed viability. Postemergence glyphosate applications at this timing provided 100% control of A. petiolata by 4 weeks after treatment at all locations whereas triclopyr efficacy was variable, providing 38-62% control. Seed production was only reduced at one location, with similar results regardless of treatment. Percent seed viability was also reduced, and when combined with reductions in seed production, we found a 71-99% reduction in number of viable seed produced plant-1 regardless of treatment. While applications did not eliminate viable seed production, our findings indicate that glyphosate and triclopyr applied while petals were dehiscing is a viable alternative to cutting or hand-pulling at this timing as it substantially decreased viable A. petiolata seed production. Management Implications Postemergence glyphosate and triclopyr applications in the early spring to rosettes are standard treatments used to manage A. petiolata. However, weather and other priorities limit the window for management, forcing field practitioners to utilize more labor-intensive methods such as hand-pulling. It is not known how late in the development of A. petiolata these herbicides can be applied to prevent viable seed production. Since prevention of soil seedbank replenishment is a key management factor for effective long-term control of biennial invasive species, we hypothesized late spring foliar herbicide applications to second year A. petiolata plants when flower petals were dehiscing could be an effective management tool if seed production or viability is eliminated. Our study indicated that glyphosate applications at this timing provided 100% control of A. petiolata plants by 4 weeks after treatment at all locations, whereas triclopyr efficacy was inconsistent. Although both glyphosate and triclopyr decreased viable seed production to nearly zero at one of our three study locations, the same treatments produced significant amounts of viable seed at the other two locations. Our findings suggest late spring glyphosate and triclopyr applications should not be recommended over early spring applications to rosettes for A. petiolata management, as our late spring application timing did not prevent viable seed production, and may require multiple years of implementation to eradicate populations. Nonetheless, this application timing holds value in areas devoid of desirable understory vegetation compared to no management practices or mechanical management options including hand-pulling when fruit are present, as overall viable seed production was reduced to similar levels as these treatments.

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Factors Limiting the Recruitment of Eucalyptus salmonophloia in Remnant Woodlands .I. Pattern of Flowering, Seed Production and Seed Fall

Australian Journal of Botany ◽

10.1071/bt9940531 ◽

1994 ◽

Vol 42 (5) ◽

pp. 531 ◽

Cited By ~ 27

Author(s):

CJ Yates ◽

RJ Hobbs ◽

RW Bell

Keyword(s):

Seed Production ◽

Western Australia ◽

Seed Viability ◽

Fragmented Landscape ◽

Viable Seed ◽

Seed Fall ◽

Individual Trees ◽

Western Australian ◽

Remnant Population ◽

Conservation Of Biodiversity

Woodlands dominated by Eucalyptus salmonophloia F. Muell. occur throughout the fragmented landscape of the Western Australian wheatbelt. In most of these remnant woodlands, there is no regeneration of E. salmonophloia and this has become a concern for the conservation of biodiversity in the region. This study examined seed production, seed viability and pattern of seed fall in four remnant populations of E. salmonophloia in the central wheatbelt of Western Australia, to determine whether these factors are limiting recruitment. Surveys of flowering, seed production and seed fall were conducted over a two-year-period which included three potential flowering seasons. Individual trees flowered once over this period and the intensity of flowering and the proportion of flowers which set seed varied greatly between remnants. Seed from up to two consecutive flowering seasons was stored in the canopy, thus, E. salmonophloia formed substantial canopy seed stores. Seeds were released from this store throughout the year and seed fall showed only a weak winter decline. The viability of seeds released from the canopy store in each remnant population was high. These results indicate that the availability of viable seed is unlikely to be responsible for the lack of E. salmonophloia recruitment in remnant woodlands.

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Effect of Herbicide Management Practices Used by Invasive Plant Managers onBerteroa incana(Hoary Alyssum) Seed Biology and Control

Invasive Plant Science and Management ◽

10.1017/inp.2018.15 ◽

2018 ◽

Vol 11 (2) ◽

pp. 101-106

Author(s):

Uriel D. Menalled ◽

Stacy C. Davis ◽

Jane M. Mangold

Keyword(s):

Population Density ◽

Seed Production ◽

Management Practices ◽

Invasive Plant ◽

Seed Viability ◽

Viable Seed ◽

Southwestern Montana ◽

Seed Biology ◽

Spraying Plants ◽

Herbicide Management

AbstractHoary alyssum [Berteroa incana(L.) DC.] is a nonnative invasive forb that is noxious in California, Idaho, Michigan, Montana, Oregon, Washington, Wyoming, Alberta, British Columbia, and Saskatchewan. ManagingB. incanais difficult, because it has an extended flowering period, during which plants simultaneously flower and produce seeds. Consequently, poorly timed herbicide applications may killB. incanaflowers but not prevent viable seed production. We examined how different herbicide management practices used by invasive plant managers affectedB. incanaseed production and viability the year of application as well as population density 1 yr after application. Professional invasive plant managers sprayedB. incanawith various herbicides as part of their current management practices at six sites in southwestern Montana in summer 2016. We collectedB. incanaplants at 4 wk postapplication for seed biology analyses. Across the six sites, nonsprayedB. incanaproduced 5 to 1,855 seeds plant−1and averaged 429 seeds plant−1. Seed production was reduced by 64% to 99% with 7 of the 11 herbicide applications.Berteroa incanaseed viability in nonsprayed areas averaged 53% and ranged from 36% to 73% across the sites. Nine of the 10 herbicide applications used by invasive plant managers reduced seed viability 49% to 100%. Few of the herbicide management practices reducedB. incana’s population density the following growing season, suggesting that managers should expect reoccurring infestations at least 1 yr after application. Our results show that invasive plant managers can reduceB. incanaviable seed production even when spraying plants that have flowered and formed seed pods. However, sites may need to be monitored for additional years to treat reoccurring infestations.

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Relative Canopy Height Influences Wild Oat (Avena fatua) Seed Viability, Dormancy, and Germination

Weed Science ◽

10.1614/ws-d-13-00084.1 ◽

2013 ◽

Vol 61 (4) ◽

pp. 564-569 ◽

Cited By ~ 2

Author(s):

Erik A. Lehnhoff ◽

Zachariah J. Miller ◽

Monica J. Brelsford ◽

Sherry White ◽

Bruce D. Maxwell

Keyword(s):

Seed Germination ◽

Seed Viability ◽

Wild Oat ◽

Viable Seed ◽

Crop Canopy ◽

Weed Seed ◽

Maternal Environment ◽

Canopy Position ◽

Oat Seeds ◽

Seed Demography

The environment in which a plant grows (maternal environment) can affect seed viability, germination, and dormancy. We assessed the effects of maternal environment on wild oat seed viability, germination, dormancy, and pathogen infection by collecting and analyzing wild oat seed from above and below a barley canopy at three field sites in Montana. The viability of wild oat seed collected below a crop canopy was consistently less than it was for seed from the overstory but varied among sites and years. Reductions in viability because of relative canopy position ranged from 10% to 30%. Effects of position relative to crop canopy on weed seed germination/dormancy rates varied by site and suggest that the direction and magnitude of the effects of maternal environment on dormancy depend on environmental conditions. These effects may be driven by crop competition or by changes in seed pathogen pressure or both. Seven species each of fungi and bacteria were isolated from wild oat seeds. The only fungi causing reductions in seed viability (15%) was isolated from understory seeds, and several bacteria from both overstory and understory sources reduced seed germination. Results suggest that, in addition to the known weed-suppressive effects of using taller or earlier emerging varieties of crops, such crops can reduce weed spread through effects on weed seed demography because weeds growing beneath the crop canopy produce a reduced amount of viable seed that is less likely to germinate in the following year.

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Seed Production and Viability of Eight Porterweed Selections Grown in Northern and Southern Florida

HortScience ◽

10.21273/hortsci.44.7.1842 ◽

2009 ◽

Vol 44 (7) ◽

pp. 1842-1849

Author(s):

Sandra B. Wilson ◽

Gary W. Knox ◽

Keona L. Muller ◽

Rosanna Freyre ◽

Zhanao Deng

Keyword(s):

Plant Growth ◽

Seed Production ◽

Seed Viability ◽

Visual Quality ◽

Ornamental Plant ◽

Viable Seed ◽

Excellent Quality ◽

Quality Ratings ◽

Southern Florida ◽

Stachytarpheta Cayennensis

Nettleleaf porterweed (Stachytarpheta cayennensis) is a potentially invasive ornamental plant in Florida. Plant growth, visual quality, flowering, and seed viability were assessed for nettleleaf porterweed and eight closely related alternatives planted in northern and southern Florida. In northern Florida, ‘Mario Pollsa’ porterweed (Stachytarpheta spp.), ‘Violacea’ porterweed (Stachytarpheta mutabilis), ‘Naples Lilac’ porterweed (Stachytarpheta spp.), ‘Red Compact’ porterweed (Stachytarpheta speciosa), and nettleleaf porterweed (Stachytarpheta cayennensis) achieved high flower ratings between 4 (average to good flowering) and 5 (abundant flowering, peak bloom) during 4 or more months. Also, jamaican porterweed (Stachytarpheta jamaicensis), ‘Violacea’ porterweed, ‘Red Compact’ porterweed, and nettleleaf porterweed achieved visual quality ratings between 4 and 5 (good to excellent quality) throughout most of the study. In southern Florida, the same cultivars received high flower ratings but generally for shorter periods of time. Also, ‘Violacea’ porterweed and ‘Red Compact’ porterweed consistently received visual quality ratings that were above 4 (good quality, very desirable). During the course of the 28-week study, nettleleaf porterweed produced the greatest number of spiked inflorescences with 39% to 80% seed viability. At both locations, ‘Violacea’ porterweed did not produce any viable seed and seed viability was less than 10% for ‘Mario Pollsa’ porterweed, coral porterweed (Stachytarpheta mutabilis), and ‘Naples Lilac’ porterweed.

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Effect of Non-Thermal Atmospheric Plasma on Micro-Tensile Bond Strength at Adhesive/Dentin Interface: A Systematic Review

Materials ◽

10.3390/ma14041026 ◽

2021 ◽

Vol 14 (4) ◽

pp. 1026

Author(s):

Mohamed M. Awad ◽

Feras Alhalabi ◽

Abdullah Alshehri ◽

Zaid Aljeaidi ◽

Ali Alrahlah ◽

...

Keyword(s):

Systematic Review ◽

Bond Strength ◽

Atmospheric Plasma ◽

Tensile Bond Strength ◽

Cochrane Library ◽

Limited Information ◽

Short Term ◽

Etch And Rinse ◽

Medium Risk

Objective: The objective of this review was to evaluate the effect of non-thermal atmospheric plasma (NTAP) on adhesives resin–dentin micro-tensile bond strength (μTBS) in previously published studies. Methods: Electronic search was conducted using the Medline, Cochrane library, and Scopus databases. The included studies were laboratory studies that investigated the effect of NTAP on adhesives μTBS to coronal dentin. Studies that evaluated the effect of NTAP on bond strength to indirect substrates, enamel or root dentin, were excluded. The methodological quality of included studies was assessed. Results: Thirteen studies were included in this systematic review. All the included studies were considered to have a medium risk of bias. NTAP significantly improved μTBS at 24 h or after short-term aging in five studies (38.5%) and both immediate and after long-term aging in 5 studies (38.5%). In two studies (15.4%), NTAP resulted in a short-term material-dependent effect that was not stable after long-term aging. Interestingly, in one study (7.7%), NTAP had a positive effect only in the etch-and-rinse (ER) mode after long-term aging. Conclusion: Within the limitations of this systematic review, NTAP application could enhance resin–dentin μTBS of ER adhesives or universal adhesives (UAs) applied in the ER mode. In the ER mode, the rewetting step after NTAP seems to be unnecessary. Because of the limited information currently available in the literature, further studies are required to evaluate the effect of the NTAP application on self-etch (SE) adhesives or UAs applied in the SE mode.

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Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

Annals of Botany ◽

10.1093/aob/mcab009 ◽

2021 ◽

Author(s):

Filippo Guzzon ◽

Maraeva Gianella ◽

Jose Alejandro Velazquez Juarez ◽

Cesar Sanchez Cano ◽

Denise E Costich

Keyword(s):

Seed Germination ◽

Plant Genetic Resources ◽

Seed Viability ◽

Storage Conditions ◽

Seed Longevity ◽

Germplasm Bank ◽

Significant Difference ◽

Different Temperatures ◽

Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

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The Effect of High Concentrations of Ethylene on Seed Germination of Douglas Fir (Pseudotsugamenziesii (Mirb.) Franco)

Canadian Journal of Forest Research ◽

10.1139/x75-058 ◽

1975 ◽

Vol 5 (3) ◽

pp. 419-423 ◽

Cited By ~ 4

Author(s):

Carey Borno ◽

Iain E. P. Taylor

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Inhibitory Effect ◽

Germination Percentage ◽

Douglas Fir ◽

Control Treatment ◽

Continuous Exposure ◽

Short Term ◽

Short Term Exposure ◽

High Concentrations

Stratified, imbibed Douglas fir (Pseudotsugamenziesii (Mirb.) Franco) seeds were exposed to 100% ethylene for times between 0 and 366 h. Germination rate and germination percentage were increased by treatments up to 48 h. The 12-h treatment gave largest stimulation; 30% enhancement of final germination percentage over control. Treatment for 96 h caused increased germination rate for the first 5 days but reduced the germination percentage. Germinants were subject to continuous exposure to atmospheres containing 0.1 – 200 000 ppm ethylene in air, but it did not stimulate growth, and the gas was inhibitory above 100 ppm. Although some effects of high concentrations of ethylene may have been due to the lowering of oxygen supplies, this alone was insufficient to account for the full inhibitory effect. The mechanism of stimulation by short-term exposure to ethylene is discussed.

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INFLUENCE DES CULTIVARS ET DES STADES DE PRÉLÈVEMENT DE LA PREMIÈRE POUSSE SUR LA PRODUCTION DE SEMENCE DE TRÈFLE ROUGE

Canadian Journal of Plant Science ◽

10.4141/cjps90-129 ◽

1990 ◽

Vol 70 (4) ◽

pp. 1071-1080 ◽

Cited By ~ 1

Author(s):

LUCIUS BELZILE

Keyword(s):

Seed Germination ◽

Seed Production ◽

Seed Yield ◽

Trifolium Pratense ◽

Red Clover ◽

Kernel Weight ◽

Climatic Conditions ◽

Cultivar Group ◽

Stage Of Development ◽

Clover Seed

For five years, the effect of cultivars and vegetative stage of cutting were measured on red clover (Trifolium pratense L.) seed production. The first group of cultivars included Hungaropoli, Ottawa and Lakeland harvested in 1981 and 1982. The second group included Arlington, Florex and Prosper I harvested in 1984, 1985 and 1987. The total annual seed yield differed within cultivars and the highest seed yield was obtained with Hungaropoly and Florex. In the first cultivar group, the tetraploid Hungaropoly obtained a kernel weight higher than the diploids Lakeland and Ottawa. In the second group of cultivars, all diploids, Arlington yielded the highest kernel weight. The percentage of seed germination of Hungaropoly was higher than Lakeland and Ottawa while for Florex it was slightly higher than Arlington and Prosper I. A prior vegetative cutting is essential for good seed yield. In the absence of vegetative cutting seed weight seemed improved. The stage of development at which vegetative cutting is done has little influence on percentage seed germination. These results have shown that despite the strong effect of climatic conditions on seed production, the choice of the optimum stage for vegetative cutting and a well adapted cultivar are important aspects in successful seed production.Key words: Red clover, seed production, cultivar, stage of cutting

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