scholarly journals Seed germination at different stratification temperatures and development of Phytelephas macrocarpa Ruiz & Pavón seedlings

2017 ◽  
Vol 39 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Sidney Alberto do Nascimento Ferreira ◽  
Daniel Felipe de Oliveira Gentil

Abstract: Phytelephas macrocarpa (ivory palm) is an Amazonian palm vulnerable to exploitation pressure, as its seeds are widely used in regional handicrafts. The aims of this study were to evaluate the effectiveness of different stratification temperatures in overcoming seed dormancy and to analyze the seedling development stages of this species. In germination under stratification, the seeds were placed in plastic bags containing moistened vermiculite, and maintained at the constant temperatures of 25, 30, 35 and 40 °C, and an alternating temperature from 26 to 40 °C. In the study about the development of seedlings, seeds were sown in vermiculite under plastic cover (growing house), and the evolution of the seedling was evaluated, from the formation of germinative button to the complete blade expansion of the first eophyll. Stratification at alternating temperatures (26 to 40 °C) helped overcoming seed dormancy. Stratification at 25 °C kept the seed viability for nine months. Germination, characterized by the formation of the germinative button took an average of 114 ± 24 days, and the seedling development until the first extended eophyll lasted 244 ± 57 days.

2017 ◽  
pp. 55
Author(s):  
Héctor Serrano-Casas ◽  
Eloy Solano-Camacho ◽  
Adelaida Ocampo-López

The seed germination process and seedling development were studied for Polianthes geminiflorn (Lex.) Rose var. geminiflom, Polianthes sp . and P. longiflom Rose. The seed viability test estimated was from 94 to 100%. In seeds treatment by soaking the seeds in water at 25ºC during three, six and twelve hours, the germination percentage, velocity and germination uniformity were increased like control assay. On the other hand, P geminiflorn var. geminiflora. were more resistent than Polianthes sp. and P.  longiflora. seeds to a treatment of three, six and twelve hours in water a t 40ºC. The three species had epigeal germination and their cotyledon developed a haustorium. None of this species exhibited seed dormancy and they were orthodox. There were significant differences in the first leave length between the three species. The seed morphology, germination behavior and seedlings development were very similar.


Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 490
Author(s):  
Saeng Geul Baek ◽  
Jin Hyun Im ◽  
Myeong Ja Kwak ◽  
Cho Hee Park ◽  
Mi Hyun Lee ◽  
...  

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.


Author(s):  
Monoj Sutradhar ◽  
Subhasis Samanta ◽  
Brijesh Kumar Singh ◽  
Md. Nasim Ali ◽  
Nirmal Mandal

Dormancy in rice serves as a mechanism of survival by protecting the seed from germinating in the mother plants; however, it becomes a problem in germination during sowing in soil or under in vitro conditions. This study was conducted to determine the effect of heat treatment and sodium hypochlorite (NaOCl) treatment of seeds on dormancy alleviation. The seeds included both freshly harvested seeds and one-year-old stored seeds, which were tested for germination after different types of seed treatments. Both the treatments increased the germination percentage in seeds, however, it was lesser in the case of old seeds. The best results were obtained from 2% NaOCl treatment for 24 hrs in new seeds, i.e. 92.84±0.103 % germination percentage (GP). However, the higher GP in old seeds were obtained from 48 hrs of heat-treated seeds i.e. 82.9±0.509 % GP. The results of the experiment revealed that rice seeds start to lose viability within a year due to seed dormancy, but this can be reversed with proper measures. These methods of breaking seed dormancy can be considered effective to break seed dormancy and improve seed germination in rice.


Botany ◽  
2015 ◽  
Vol 93 (12) ◽  
pp. 881-892 ◽  
Author(s):  
Valentina Murru ◽  
Andrea Santo ◽  
Carol Piazza ◽  
Laetitia Hugot ◽  
Gianluigi Bacchetta

The Silene mollissima (L.) Pers. aggregate is part of section Siphonomorpha Otth. and currently comprises 11 narrow endemic species of the Western Mediterranean Basin. Three of these taxa (S. velutina Pourr. ex Loisel, S. ichnusae Brullo, De Marco & De Marco f., and S. badaroi Breistr.) have a distribution range centred in the northern Tyrrhenian area and occurring in coastal habitats. Inter- and intra-specific variability in the responses to light, constant (5–25 °C) and alternating temperatures (25/10 °C), salt (NaCl, 0–600 mmol/L), and levels of nitrate (KNO3, 20 mmol/L) under salt stress, as well as recovery of seed germination were evaluated for these species to more effectively support their in-situ and ex-situ conservation. Our results highlighted that the seeds of these three taxa were nondormant, and that light significantly improved their rate of germination, which was higher (>80%) at low temperatures (5–15 °C) and under the alternating temperature regime (25/10 °C), but decreased significantly at the highest temperature tested (25 °C). Seeds from Silene velutina and S. ichnusae germinated in up to 300 mmol/L NaCl, and S. badaroi germinated in up to 100 mmol/L. For all of the species except S. badaroi, salt did not affect seed viability, and recovery germination did not decrease with increasing salinity and temperature. Interpopulation variability, both in salt tolerance and recovery germination, was detected for S. velutina. The addition of KNO3 did not affect germination or recovery germination under salt conditions. The lack of effect from KNO3 suggests that nutrient availability is not a requirement for seed germination in these species. Our results show that all species experience an optimum period of germination during autumn–winter, which is when water availability is highest and soil salinity levels are minimal because of the Mediterranean rainfalls, but seeds from S. velutina and S. ichnusae will germinate up until spring.


2010 ◽  
Vol 28 (3) ◽  
pp. 166-172
Author(s):  
Cary J. Hebert ◽  
Frank A. Blazich ◽  
Anthony V. LeBude

Abstract Seeds from five populations of Rhododendron vaseyi A. Gray (pinkshell azalea), representing the entire distribution of the species, were germinated at 25C (77F) or an 8/16-hr thermoperiod of 30/20C (86/68F) with daily photoperiods at each temperature of 0 (total darkness), 8, 12, or 24-hr (continuous light). Germination was recorded every 3 days for 30 days. Responses to light and temperature of all populations were similar. Light was required for germination regardless of temperature. As photoperiod increased, germination increased for all populations with the alternating temperature partially compensating for the light requirement. The highest cumulative germination for all populations ranged from 51 to 67% and was achieved at 30/20C with a 24-hr photoperiod. These germination percentages, although at a moderate level, were due in part to rigorous cleaning and grading of seeds collected across a broad range of plants and growing conditions prior to initiation of the study, suggesting seed viability of R. vaseyi is inherently low.


2018 ◽  
Vol 1 (1) ◽  
pp. 09-18
Author(s):  
Indra Dwipa ◽  
Muhsanati Muhsanati

Most of rice seeds experience after ripening problem which then result in seed dormancy. This kind of dormancy could be broken through various types of treatments, including storaging in dry place for certain period. This study was aimed to evaluate the combination between seed water content and storage duration to induce the seed germination of local genotype brown rice, Daro Merah. Various levels of seed water content (6-10, 11-15, 16-20 and 21-25 %) and storage duration (2, 4, 6 and 8 weeks) were evaluated to identify its effect on Daro Merah seed germination. Data were analyzed using two-way analysis of variance where significance were further proceeded using Duncan’s New Multiple Range Test with a p>0.05. The results showed that seed germination of Daro Merah reached 95 % at five days after seeding when the seed water content was maintained at 6-10% and stored for 8 weeks. After 14 days, the germination reached 99 % and most of the seeds showed some shoots emerged from the soil. Keywords: Brown rice, germination, seed water content, storage duration, Daro Merah


2008 ◽  
Vol 24 (6) ◽  
pp. 619-627 ◽  
Author(s):  
Mercedes S. Foster

Abstract:The relative effectiveness of arboreal or terrestrial birds at dispersing seeds of Ormosia macrocalyx and O. bopiensis (Fabaceae: Papilionoideae) were studied in south-eastern Peru. Seeds of both species were either scarified, to represent seed condition after dispersal by terrestrial birds, or left intact, to represent seed condition after dispersal by arboreal birds. Seeds were distributed along forest transects, and germination, seedling development and mortality were monitored to determine the successes of the two groups at producing seedlings. Scarified seeds germinated with the early rains of the dry-to-wet-season transition, when erratic rainfall was interspersed with long dry spells. Intact seeds germinated 30 d later when the rain was more plentiful and regular. Intact seeds of O. macrocalyx gave rise to significantly more seedlings (41.1% vs. 25.5%) than did scarified seeds, in part, because significantly more seedlings from scarified seeds (n = 20) than from intact seeds (n = 3) died from desiccation when their radicles failed to enter the dry ground present during the dry-to-wet-season transition. Also, seedlings from scarified seeds were neither larger nor more robust than those from intact seeds despite their longer growing period. Results are consistent with the hypothesis that dispersal effectiveness of arboreal birds, at least for O. macrocalyx, is greater than that of terrestrial birds. Screen-house experiments in which seedlings developed under different watering regimes supported this result. Numbers of seedlings developing from intact and scarified seeds of O. bopiensis did not differ significantly.


2011 ◽  
Vol 76 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Tadeusz Tylkowski

European bladder nut has its northern limit in Poland and is legally protected. Climatic conditions, edible seeds attractive for small animals and seed dormancy to a large extent affect its restocking under natural conditions. The aim of this study was to determine conditions for seed dormancy release. Nuts dried after collection to ca 11% of moisture content (fresh weight basis) may be stored without loss of seed viability for over 1 year in a cold store at the temperature of -3<sup>o</sup>C. For the purpose of seed dormancy release nuts need to be stratified. Seed dormancy release was found highest after the application of warm-followed-by-cold stratification, first for 4-6 weeks at the temperature of 15<sup>o</sup>C or at cyclically alternating temperature of 10~20<sup>o</sup>C (24+24 h/cycle), followed by 16-18 weeks at 3<sup>o</sup>C. Seeds germinate at 3<sup>o</sup>C with the same rate as at cyclically alternating temperature of 3~15<sup>o</sup>C (16+8 h/day). Drying of nuts at room temperature to approx. 11% during the warm stratification phase (after 2 or 4 weeks) and further stratification resulted in a significant increase in seed germinability. In some seed lots scarification of nuts (dried during the warm stratification phase) contributes to a further significant increase of seed germinability.


2021 ◽  
Vol 146 (1) ◽  
pp. 50-55
Author(s):  
Michael Stein ◽  
Corina Serban ◽  
Per McCord

Seeds of sweet cherry (Prunus avium) possess a strong endodormancy response that traditionally requires months of stratification before germination can occur. Accelerating artificial dormancy release could be an important aspect of improving progress in a sweet cherry breeding program by increasing the first season growth period. In this study, seeds were exogenously treated with ethephon, 1-aminocyclopropane-1-carboxylic acid (ACC), kinetin, hydrogen peroxide, and acidified nitrite to attempt to induce germination with reduced stratification times. Findings showed that ethephon, ACC, and hydrogen peroxide all significantly increase early seed germination rates. Stratification time had the largest effect on increasing germinations, with significantly higher germination percentages accompanying increased stratification times that plateaued at 3 months of stratification. Stratification is vital for proper seedling development because plants grown from seeds with no stratification are significantly shorter and have reduced internode lengths compared with seeds with 4 weeks of stratification.


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