Magnesium Uptake by the Green Microalga Chlorella vulgaris in Batch Cultures

Acetate can be efficiently metabolized by the green microalga Chlamydomonas reinhardtii. The regular concentration is 17 mM, although higher concentrations are reported to increase starch and fatty acid content. To understand the responses to higher acetate concentrations, Chlamydomonas cells were cultivated in batch mode in the light at 17, 31, 44, and 57 mM acetate. Metabolic analyses show that cells grown at 57 mM acetate possess increased contents of all components analyzed (starch, chlorophylls, fatty acids, and proteins), with a three-fold increased volumetric biomass yield compared to cells cultivated at 17 mM acetate at the entry of stationary phase. Physiological analyses highlight the importance of photosynthesis for the low-acetate and exponential-phase samples. The stationary phase is reached when acetate is depleted, except for the cells grown at 57 mM acetate, which still divide until ammonium exhaustion. Surprisal analysis of the transcriptomics data supports the biological significance of our experiments. This allows the establishment of a model for acetate assimilation, its transcriptional regulation and the identification of candidates for genetic engineering of this metabolic pathway. Altogether, our analyses suggest that growing at high-acetate concentrations could increase biomass productivities in low-light and CO2-limiting air-bubbled medium for biotechnology.

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Oral Administration of an Enzymatic Protein Hydrolysate from the Green Microalga Chlorella vulgaris Enhances the Nutritional Recovery of Malnourished Mice

Journal of Medicinal Food ◽

10.1089/jmf.2010.0283 ◽

2011 ◽

Vol 14 (12) ◽

pp. 1583-1589 ◽

Cited By ~ 4

Author(s):

Humberto J. Morris ◽

Olimpia V. Carrillo ◽

María E. Alonso ◽

Rosa C. Bermúdez ◽

Ángel Almarales ◽

...

Keyword(s):

Oral Administration ◽

Chlorella Vulgaris ◽

Protein Hydrolysate ◽

Nutritional Recovery ◽

Green Microalga

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Comparison of the toxicity of pure and samarium-doped zinc oxide nanoparticles to the green microalga Chlorella vulgaris

Environmental Science and Pollution Research ◽

10.1007/s11356-022-18539-x ◽

2022 ◽

Author(s):

Sanaz Feizi ◽

Morteza Kosari-Nasab ◽

Baharak Divband ◽

Sepideh Mahjouri ◽

Ali Movafeghi

Keyword(s):

Zinc Oxide ◽

Chlorella Vulgaris ◽

Zinc Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Green Microalga

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Mapping the Fundamental Niches of Two Freshwater Microalgae,Chlorella vulgaris(Trebouxiophyceae) andPeridinium cinctum(Dinophyceae), in 5-Dimensional Ion Space

International Journal of Ecology ◽

10.1155/2011/738035 ◽

2011 ◽

Vol 2011 ◽

pp. 1-12 ◽

Cited By ~ 2

Author(s):

Terence J. Evens ◽

Randall P. Niedz

Keyword(s):

Growth Rate ◽

Experimental Design ◽

Chlorella Vulgaris ◽

Growth Rates ◽

Ion Concentration ◽

Freshwater Microalgae ◽

Batch Cultures ◽

Fundamental Niche ◽

Maximum Cell ◽

Cell Densities

The fundamental niche defined by five ions,NO3 −,PO4 3−, K+, Na+, andCl−, was mapped forChlorella vulgaris(Trebouxiophyceae) andPeridinium cinctum(Dinophyceae) growth rates and maximum cell densities in batch cultures. A five dimensional ion-mixture experimental design was projected across a total ion concentration gradient of 1 to 30 mM to delineate the ion-based, “potential” niche space, defined as the entiren-dimensional hypervolume demarcated by the feasible ranges of the independent factors under consideration. The growth rate-based, fundamental niche volumes overlapped for ca. 94% of the ion mixtures, although the regions of maximal growth rates and cell densities were different for each alga. BothC. vulgarisandP. cinctumexhibited similar positive responses to cations and negative responses to anions. It was determined that total ion concentration for these five ions, from 1 to 30 mM, did not directly affect either growth rate or maximal cell density for either alga, although it did play an interactive role with several ions. This study is the first that we are aware of to attempt the mapping of a multivariate, ion-based, fundamental niche volume. The implications of the experimental design utilized and the potential utility of this type of approach are discussed.

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