Bifidobacterium adolescentis P2P3, a Human Gut Bacterium Having Strong Non-Gelatinized Resistant Starch-Degrading Activity

The digestion of four sources of resistant starch (RS) has been studied in twelve healthy volunteers who ate controlled diets for 15 d periods. RS from potato, banana, wheat and maize (17−30 g/d) was compared with a starch-free diet, a diet containing wheat starch that was fully digested in the small intestine, and with 18·4 g NSP from bran/d. RS increased stool wet weight by 1·6 g/d per g RS fed for potato, 1·7 for banana, 2·5 for wheat and 2·7 for maize, but this was significantly less than bran NSP at 4·9 g/g. RS was extensively digested in twenty-seven of thirtyfour diet periods but five subjects were unable to break down one or two of the RS sources. Faecal N and energy excretion were increased. RS decreased NSP breakdown and RS2(resistant starch granules) tended to prolong transit time. All forms of RS increased faecal total short-chain fatty acid excretion. RS2(from potato and banana) gave greater proportions of acetate in faeces, and RS3(retrograded starch from wheat and maize) more propionate. We have concluded that RS2and RS3are broken down in the human gut, probably in the colon although in 26% of cases this breakdown was impaired. RS exerts mild laxative properties, predominantly through stimulation of biomass excretion but also through some sparing of NSP breakdown.

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Human gut commensal bacterium Ruminococcus species FMB-CY1 completely degrades the granules of resistant starch

Food Science and Biotechnology ◽

10.1007/s10068-021-01027-2 ◽

2022 ◽

Author(s):

Yeong-Sik Hong ◽

Dong-Hyun Jung ◽

Won-Hyong Chung ◽

Young-Do Nam ◽

Ye-Jin Kim ◽

...

Keyword(s):

Resistant Starch ◽

Commensal Bacterium ◽

Human Gut

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Impact of Resistant Maltodextrins and Resistant Starch on Human Gut Microbiota and Organic Acids Production

Starch - Stärke ◽

10.1002/star.201800231 ◽

2019 ◽

pp. 1800231 ◽

Cited By ~ 3

Author(s):

Waraporn Sorndech ◽

Sureelak Rodtong ◽

Andreas Blennow ◽

Sunanta Tongta

Keyword(s):

Gut Microbiota ◽

Organic Acids ◽

Resistant Starch ◽

Human Gut ◽

Human Gut Microbiota

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Bifidobacterium adolescentis shows potential to strengthen host defence against gastrointestinal infection via inhibition of the opportunistic pathogen Candida albicans and stimulation of human-isolated macrophages killing capacity in vitro

Access Microbiology ◽

10.1099/acmi.ac2020.po0743 ◽

2020 ◽

Vol 2 (7A) ◽

Author(s):

Liviana Ricci ◽

Joanna Mackie ◽

Megan D. Lenardon ◽

Caitlin Jukes ◽

Ahmed N. Hegazy ◽

...

Keyword(s):

Candida Albicans ◽

Bacterial Species ◽

Opportunistic Pathogen ◽

Limited Information ◽

Human Gut ◽

Bifidobacterium Adolescentis ◽

Host Immune Responses ◽

Opportunistic Fungal Pathogen ◽

Antimicrobial Metabolites

The human gut microbiota enhances the host’s resistance to enteric pathogens via colonisation resistance, a phenomenon that is driven by multiple mechanisms, such as production of antimicrobial metabolites and activation of host immune responses. However, there is limited information on how individual gut bacterial species, particularly many of the dominant anaerobes, might impact the host’s defence. This study investigated the potential of specific human gut isolates to bolster the host’s resistance to infection. First, by antagonising the opportunistic fungal pathogen Candida albicans, and secondly, by modulating the killing capacity of human-isolated macrophages in vitro. Co-culturing C. albicans with faecal microbiota from different healthy individuals revealed varying levels of fungal inhibition. In vitro assays with a panel of representative human gut anaerobes confirmed that culture supernatants from certain bacterial isolates, in particular of Bifidobacterium adolescentis, significantly inhibited C. albicans growth. Mechanistic studies revealed that microbial fermentation acids including acetate and lactate, in combination with the associated decrease in pH, were strong drivers of this inhibitory activity. In the second in vitro assay, human-isolated macrophages were exposed to bacterial supernatants, and subsequently tested for their capacity to eliminate adherent-invasive Escherichia coli. Among the gut anaerobes tested, B. adolescentis was revealed to exert the strongest immunostimulatory and killing effect when compared to the unstimulated macrophages control. B. adolescentis is known to be stimulated by dietary consumption of resistant starch andmay therefore represent an attractive target for the development of probiotic and prebiotic interventions tailored to enhancethe host’s natural defences against infection.

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Enzymatic analysis of truncation mutants of a type II pullulanase from Bifidobacterium adolescentis P2P3, a resistant starch-degrading gut bacterium

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2021.10.193 ◽

2021 ◽

Author(s):

Sun-Young Kim ◽

Hyeran Kim ◽

Ye-Jin Kim ◽

Dong-Hyun Jung ◽

Dong-Ho Seo ◽

...

Keyword(s):

Resistant Starch ◽

Type Ii ◽

Enzymatic Analysis ◽

Bifidobacterium Adolescentis

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Identifying species of symbiont bacteria from the human gut that, alone, can induce intestinal Th17 cells in mice

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1617460113 ◽

2016 ◽

Vol 113 (50) ◽

pp. E8141-E8150 ◽

Cited By ~ 156

Author(s):

Tze Guan Tan ◽

Esen Sefik ◽

Naama Geva-Zatorsky ◽

Lindsay Kua ◽

Debdut Naskar ◽

...

Keyword(s):

Th17 Cell ◽

Th17 Cells ◽

Bacterial Species ◽

Alternative Mechanism ◽

Filamentous Bacteria ◽

Transcriptional Program ◽

Human Gut ◽

Bifidobacterium Adolescentis ◽

Gut Epithelium ◽

Cell Accumulation

Th17 cells accrue in the intestine in response to particular microbes. In rodents, segmented filamentous bacteria (SFB) induce intestinal Th17 cells, but analogously functioning microbes in humans remain undefined. Here, we identified human symbiont bacterial species, in particularBifidobacterium adolescentis, that could, alone, induce Th17 cells in the murine intestine. Similar to SFB,B. adolescentiswas closely associated with the gut epithelium and engendered cognate Th17 cells without attendant inflammation. However,B. adolescentiselicited a transcriptional program clearly distinct from that of SFB, suggesting an alternative mechanism of promoting Th17 cell accumulation. Inoculation of mice withB. adolescentisexacerbated autoimmune arthritis in the K/BxN mouse model. Several off-the-shelf probiotic preparations that includeBifidobacteriumstrains also drove intestinal Th17 cell accumulation.

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Bifidobacterium adolescentis as a key member of the human gut microbiota in the production of GABA

Scientific Reports ◽

10.1038/s41598-020-70986-z ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

Sabrina Duranti ◽

Lorena Ruiz ◽

Gabriele Andrea Lugli ◽

Héctor Tames ◽

Christian Milani ◽

...

Keyword(s):

Gut Microbiota ◽

Human Gut ◽

Bifidobacterium Adolescentis ◽

Human Gut Microbiota

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Two Routes of Metabolic Cross-Feeding between Bifidobacterium adolescentis and Butyrate-Producing Anaerobes from the Human Gut

Applied and Environmental Microbiology ◽

10.1128/aem.72.5.3593-3599.2006 ◽

2006 ◽

Vol 72 (5) ◽

pp. 3593-3599 ◽

Cited By ~ 432

Author(s):

Alvaro Belenguer ◽

Sylvia H. Duncan ◽

A. Graham Calder ◽

Grietje Holtrop ◽

Petra Louis ◽

...

Keyword(s):

Pure Culture ◽

Dietary Carbohydrates ◽

Human Gut ◽

Bifidobacterium Adolescentis ◽

Acetyl Coenzyme A ◽

End Products ◽

Pure Cultures ◽

Human Large Intestine ◽

Eubacterium Hallii

ABSTRACT Dietary carbohydrates have the potential to influence diverse functional groups of bacteria within the human large intestine. Of 12 Bifidobacterium strains of human gut origin from seven species tested, four grew in pure culture on starch and nine on fructo-oligosaccharides. The potential for metabolic cross-feeding between Bifidobacterium adolescentis and lactate-utilizing, butyrate-producing Firmicute bacteria related to Eubacterium hallii and Anaerostipes caccae was investigated in vitro. E. hallii L2-7 and A. caccae L1-92 failed to grow on starch in pure culture, but in coculture with B. adolescentis L2-32 butyrate was formed, indicating cross-feeding of metabolites to the lactate utilizers. Studies with [13C]lactate confirmed carbon flow from lactate, via acetyl coenzyme A, to butyrate both in pure cultures of E. hallii and in cocultures with B. adolescentis. Similar results were obtained in cocultures involving B. adolescentis DSM 20083 with fructo-oligosaccharides as the substrate. Butyrate formation was also stimulated, however, in cocultures of B. adolescentis L2-32 grown on starch or fructo-oligosaccharides with Roseburia sp. strain A2-183, which produces butyrate but does not utilize lactate. This is probably a consequence of the release by B. adolescentis of oligosaccharides that are available to Roseburia sp. strain A2-183. We conclude that two distinct mechanisms of metabolic cross-feeding between B. adolescentis and butyrate-forming bacteria may operate in gut ecosystems, one due to consumption of fermentation end products (lactate and acetate) and the other due to cross-feeding of partial breakdown products from complex substrates.

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