Human gut commensal bacterium Ruminococcus species FMB-CY1 completely degrades the granules of resistant starch

2022 ◽  
Author(s):  
Yeong-Sik Hong ◽  
Dong-Hyun Jung ◽  
Won-Hyong Chung ◽  
Young-Do Nam ◽  
Ye-Jin Kim ◽  
...  
Keyword(s):  
Resistant Starch ◽  
Commensal Bacterium ◽  
Human Gut

Digestion and physiological properties of resistant starch in the human large bowel

1996 ◽  
Vol 75 (5) ◽  
pp. 733-747 ◽  
Author(s):  
John H Cummings ◽  
Emily R Beatty ◽  
Susan M Kingman ◽  
Sheila A Bingham ◽  
Hans N Englyst
Keyword(s):  
Resistant Starch ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Wheat Starch ◽  
Starch Granules ◽  
Human Gut ◽  
Physiological Properties ◽  
Retrograded Starch ◽  
Wet Weight ◽  
Stimulation Of

The digestion of four sources of resistant starch (RS) has been studied in twelve healthy volunteers who ate controlled diets for 15 d periods. RS from potato, banana, wheat and maize (17−30 g/d) was compared with a starch-free diet, a diet containing wheat starch that was fully digested in the small intestine, and with 18·4 g NSP from bran/d. RS increased stool wet weight by 1·6 g/d per g RS fed for potato, 1·7 for banana, 2·5 for wheat and 2·7 for maize, but this was significantly less than bran NSP at 4·9 g/g. RS was extensively digested in twenty-seven of thirtyfour diet periods but five subjects were unable to break down one or two of the RS sources. Faecal N and energy excretion were increased. RS decreased NSP breakdown and RS2(resistant starch granules) tended to prolong transit time. All forms of RS increased faecal total short-chain fatty acid excretion. RS2(from potato and banana) gave greater proportions of acetate in faeces, and RS3(retrograded starch from wheat and maize) more propionate. We have concluded that RS2and RS3are broken down in the human gut, probably in the colon although in 26% of cases this breakdown was impaired. RS exerts mild laxative properties, predominantly through stimulation of biomass excretion but also through some sparing of NSP breakdown.

Impact of Resistant Maltodextrins and Resistant Starch on Human Gut Microbiota and Organic Acids Production

2019 ◽  
pp. 1800231 ◽  
Author(s):  
Waraporn Sorndech ◽  
Sureelak Rodtong ◽  
Andreas Blennow ◽  
Sunanta Tongta
Keyword(s):  
Gut Microbiota ◽  
Organic Acids ◽  
Resistant Starch ◽  
Human Gut ◽  
Human Gut Microbiota

scholarly journals Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity

3 Biotech ◽  
2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Dong-Hyun Jung ◽  
Won-Hyong Chung ◽  
Dong-Ho Seo ◽  
Ye-Jin Kim ◽  
Young-Do Nam ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Resistant Starch ◽  
Complete Genome ◽  
Human Gut ◽  
Bifidobacterium Adolescentis

scholarly journals Impact of dietary resistant starch type 4 on human gut microbiota and immunometabolic functions

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Bijaya Upadhyaya ◽  
Lacey McCormack ◽  
Ali Reza Fardin-Kia ◽  
Robert Juenemann ◽  
Sailendra Nichenametla ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Resistant Starch ◽  
Human Gut ◽  
Human Gut Microbiota

Effects of Resistant Starch Type III Polymorphs on Human Colon Microbiota and Short Chain Fatty Acids in Human Gut Models

2008 ◽  
Vol 56 (13) ◽  
pp. 5415-5421 ◽  
Author(s):  
Uri Lesmes ◽  
Emma J. Beards ◽  
Glenn R. Gibson ◽  
Kieran M. Tuohy ◽  
Eyal Shimoni
Keyword(s):  
Fatty Acids ◽  
Resistant Starch ◽  
Human Colon ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Human Gut ◽  
Type Iii ◽  
Chain Fatty Acids

scholarly journals Bifidobacterium adolescentis P2P3, a Human Gut Bacterium Having Strong Non-Gelatinized Resistant Starch-Degrading Activity

2019 ◽  
Vol 29 (12) ◽  
pp. 1904-1915 ◽  
Author(s):  
Dong-Hyun Jung ◽  
Ga-Young Kim ◽  
In-Young Kim ◽  
Dong-Ho Seo ◽  
Young-Do Nam ◽  
...  
Keyword(s):  
Resistant Starch ◽  
Human Gut ◽  
Bifidobacterium Adolescentis

The presence of resistant starch-degrading amylases in Bifidobacterium adolescentis of the human gut

2020 ◽  
Vol 161 ◽  
pp. 389-397 ◽  
Author(s):  
Dong-Hyun Jung ◽  
Dong-Ho Seo ◽  
Ye-Jin Kim ◽  
Won-Hyong Chung ◽  
Young-Do Nam ◽  
...  
Keyword(s):  
Resistant Starch ◽  
Human Gut ◽  
Bifidobacterium Adolescentis

scholarly journals Cas9-Assisted Biological Containment of a Genetically Engineered Human Commensal Bacterium and Genetic Elements

2021 ◽  
Author(s):  
Naoki Hayashi ◽  
Yong Lai ◽  
Mark Mimee ◽  
Timothy K Lu
Keyword(s):  
Gene Expression ◽  
Gene Cassette ◽  
Genetically Engineered ◽  
Commensal Bacterium ◽  
Human Gut ◽  
Genetic Elements ◽  
Thymidine Auxotrophy ◽  
Engineered Bacteria

Sophisticated gene circuits built by synthetic biology can enable bacteria to sense their environment and respond predictably. Biosensing bacteria can potentially probe the human gut microbiome to prevent, diagnose, or treat disease. To provide robust biocontainment for engineered bacteria, we devised a Cas9-assisted auxotrophic biocontainment system combining thymidine auxotrophy, an Engineered Riboregulator (ER) for controlled gene expression, and a CRISPR Device (CD). The CD prevents the engineered bacteria from acquiring thyA via horizontal gene transfer, which would disrupt the biocontainment system, and inhibits the spread of genetic elements by killing bacteria harboring the gene cassette. This system tunably controlled gene expression in the human gut commensal bacterium Bacteroides thetaiotaomicron, prevented escape from thymidine auxotrophy, and blocked transgene dissemination for at least 10 days. These capabilities were validated in vitro and in vivo. This biocontainment system exemplifies a powerful strategy for bringing genetically engineered microorganisms safely into biomedicine.

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