scholarly journals Population dynamics of Saccharomyces cerevisiae PE-2 and CAT-1 in CO-culture for the production of ethanol

2020 ◽  
Vol 42 ◽  
pp. e43427
Author(s):  
Mayara Vieira Santos ◽  
Adriana Régia Marques Souza ◽  
Maria Carolina Santos Silva ◽  
Gabriel Luis Castiglioni
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Population Dynamics ◽  
Acetic Acid ◽  
Ethanol Production ◽  
Metabolic Pathways ◽  
The Other ◽  
Yeast Strains ◽  
Pure Cultures ◽  
Fermentation Monitoring ◽  
Biotechnological Processes

In the Brazilian industries, the inoculum used throughout the harvest of ethanol production consists of a combination of two or more yeast strains. The combination of yeasts may influence in the metabolic pathways of microorganisms and increase the yields and production rates of some compounds. In biotechnological processes with co-culture, one microorganism can prevail over the other. Therefore, the knowledge about how the population dynamics occurs during fermentation allows modifications in the process in order to obtain higher yields and to achieve greater fermentative efficiency. The aim of this study was to investigate the fermentation with synthetic sugar cane broth in co-culture of Saccharomyces cerevisiae strains CAT-1 and PE-2 followed by molecular fermentation monitoring. The concentration of biomass, ethanol, glycerol, acetic acid and residual sucrose were monitored to verify the influence of different combinations during the fermentation. The mixture of CAT-1 and PE-2 presented the highest ethanol production, with higher performance of fermentative parameters than pure cultures

scholarly journals Endogenous lycopene improves ethanol production under acetic acid stress in Saccharomyces cerevisiae

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Shuo Pan ◽  
Bin Jia ◽  
Hong Liu ◽  
Zhen Wang ◽  
Meng-Zhe Chai ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Ethanol Production ◽  
Acid Stress

scholarly journals Two Novel Techniques for Determination of Polysaccharide Cross-Links Show that Crh1p and Crh2p Attach Chitin to both β(1-6)- and β(1-3)Glucan in the Saccharomyces cerevisiae Cell Wall

2009 ◽  
Vol 8 (11) ◽  
pp. 1626-1636 ◽  
Author(s):  
Enrico Cabib
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Acetic Acid ◽  
Yeast Cell ◽  
Cell Walls ◽  
Double Mutant ◽  
The Other ◽  
Cross Links

ABSTRACT Previous work, using solubilization of yeast cell walls by carboxymethylation, before or after digestion with β(1-3)- or β(1-6)glucanase, followed by size chromatography, showed that the transglycosylases Crh1p and Crh2p/Utr2p were redundantly required for the attachment of chitin to β(1-6)glucan. With this technique, crh1Δ crh2Δ mutants still appeared to contain a substantial percentage of chitin linked to β(1-3)glucan. Two novel procedures have now been developed for the analysis of polysaccharide cross-links in the cell wall. One is based on the affinity of curdlan, a β(1-3)glucan, for β(1-3)glucan chains in carboxymethylated cell walls. The other consists of in situ deacetylation of cell wall chitin, generating chitosan, which can be extracted with acetic acid, either directly (free chitosan) or after digestion with different glucanases (bound chitosan). Both methodologies indicated that all of the chitin in crh1Δ crh2Δ strains is free. Reexamination of the previously used procedure revealed that the β(1-3)glucanase preparation used (zymolyase) is contaminated with a small amount of endochitinase, which caused erroneous results with the double mutant. After removing the chitinase from the zymolyase, all three procedures gave coincident results. Therefore, Crh1p and Crh2p catalyze the transfer of chitin to both β(1-3)- and β(1-6)glucan, and the biosynthetic mechanism for all chitin cross-links in the cell wall has been established.

scholarly journals Ancient balancing selection maintains incompatible versions of the galactose pathway in yeast

Science ◽  
2021 ◽  
Vol 371 (6527) ◽  
pp. 415-419
Author(s):  
James Boocock ◽  
Meru J. Sadhu ◽  
Arun Durvasula ◽  
Joshua S. Bloom ◽  
Leonid Kruglyak
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Pathways ◽  
Genetic Interaction ◽  
Balancing Selection ◽  
The Other ◽  
Reference Allele ◽  
Trade Off ◽  
Galactose Utilization

Metabolic pathways differ across species but are expected to be similar within a species. We discovered two functional, incompatible versions of the galactose pathway in Saccharomyces cerevisiae. We identified a three-locus genetic interaction for growth in galactose, and used precisely engineered alleles to show that it arises from variation in the galactose utilization genes GAL2, GAL1/10/7, and phosphoglucomutase (PGM1), and that the reference allele of PGM1 is incompatible with the alternative alleles of the other genes. Multiloci balancing selection has maintained the two incompatible versions of the pathway for millions of years. Strains with alternative alleles are found primarily in galactose-rich dairy environments, and they grow faster in galactose but slower in glucose, revealing a trade-off on which balancing selection may have acted.

scholarly journals Crossbreeding of Yeasts Domesticated for Fermentation: Infertility Challenges

2020 ◽  
Vol 21 (21) ◽  
pp. 7985
Author(s):  
Nobuo Fukuda
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sexual Reproduction ◽  
Genetic Information ◽  
Yeast Saccharomyces Cerevisiae ◽  
Yeast Strains ◽  
Fermentative Production ◽  
Universal Feature ◽  
Ancient Times ◽  
Biotechnological Processes ◽  
Eukaryotic Organisms

Sexual reproduction is almost a universal feature of eukaryotic organisms, which allows the reproduction of new organisms by combining the genetic information from two individuals of different sexes. Based on the mechanism of sexual reproduction, crossbreeding provides an attractive opportunity to improve the traits of animals, plants, and fungi. The budding yeast Saccharomyces cerevisiae has been widely utilized in fermentative production since ancient times. Currently it is still used for many essential biotechnological processes including the production of beer, wine, and biofuels. It is surprising that many yeast strains used in the industry exhibit low rates of sporulation resulting in limited crossbreeding efficiency. Here, I provide an overview of the recent findings about infertility challenges of yeasts domesticated for fermentation along with the progress in crossbreeding technologies. The aim of this review is to create an opportunity for future crossbreeding of yeasts used for fermentation.

scholarly journals The Production of Metabolites by Saccharomyces Cerevisiae and its Application in Biotechnological Processes

2021 ◽  
Vol 10 (3) ◽  
pp. 174-184
Author(s):  
Maria do Socorro Mascarenhas Santos ◽  
Margareth Batistote ◽  
Claudia Andrea Lima Cardoso
Keyword(s):  
United States ◽  
Saccharomyces Cerevisiae ◽  
Metabolic Pathways ◽  
The United States ◽  
Publication Rate ◽  
Scientific Production ◽  
Added Value ◽  
Research Articles ◽  
Metabolic Capacity ◽  
Biotechnological Processes

Saccharomyces cerevisiae yeasts are widely known and used in biotechnological processes, as they have an excellent metabolic capacity that results in the formation of natural products with high added value. Thus, this study aims to present a view on the production of metabolites by Saccharomyces cerevisiae and their application in biotechnological processes. For this, a bibliometric analysis was carried out on the scientific production regarding the use of yeasts in biotechnological tests for the production of substances by activating their metabolic pathways. The articles found in the range between the years 2014 to 2019 are mostly research articles 57% and the rest 43% review. The analysis of the production of articles per year showed an oscillation for both research and review articles, and the countries with the highest publication rate are the United States and China. The data demonstrate a growing interest in secondary metabolic pathways of S. cerevisiae. These microorganisms can be used for the production of different metabolites that are of industrial interest, as they have a purity content that results in high commercial value.

scholarly journals Assessment of the Effect of Nitrogen Concentration on Fermentation and Selection of a Highly Competitive Saccharomyces cerevisiae Strain for Efficient Ethanol Production

Energies ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2614
Author(s):  
Barahona ◽  
Martín-Gil ◽  
Martín-Ramos ◽  
Pérez ◽  
Barriga
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Yield ◽  
Nitrogen Concentration ◽  
Glucose Consumption ◽  
Cellular Growth ◽  
Yeast Strains ◽  
Two Factors ◽  
Pilot Plant Scale ◽  
Reducing Costs

: The optimum nitrogen concentration for media supplementation and strain dominance are aspects of key importance to the industrial production of ethanol with a view to reducing costs and increasing yields. In this work, these two factors were investigated for four ethanologenic Saccharomyces cerevisiae strains (CLQCA-INT-001, CLQCA-INT-005, CLQCA-10-099, and UCLM 325), selected from the screening of 150 isolates, mostly from Ecuadorian yeast biodiversity. The effect of nitrogen concentration was assessed in terms of cellular growth, glucose consumption and ethanol production, and the yeast strains’ dominance was evaluated in continuous co-fermentation with cellular recycling by mitochondrial DNA analyses. Among the four selected yeast strains under study, CLQCA-INT-005 presented the highest glucose consumption at a nitrogen supplement concentration as low as 0.4 g·L−1, attaining an ethanol yield of up to 96.72% in 24 h. The same yeast strain was found to be highly competitive, showing a dominance of 80% after four cycles of fermentation in co-culture. Thus, CLQCA-INT-005 may be deemed as a very promising candidate to be used both at pilot-plant scale and at industrial scale cellulosic ethanol production.

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