In Vivo and In Vitro Response to a Gelatin/α-Tricalcium Phosphate Bone Cement

2007 ◽  
pp. 1001-1004
Author(s):  
Barbara Bracci ◽  
Milena Fini ◽  
Silvia Panzavolta ◽  
Paola Torricelli ◽  
Adriana Bigi
Keyword(s):  
Bone Cement ◽  
Tricalcium Phosphate ◽  
In Vitro Response

In Vivo and In Vitro Response to a Gelatin/α-Tricalcium Phosphate Bone Cement

2007 ◽  
Vol 361-363 ◽  
pp. 1001-1004 ◽  
Author(s):  
Barbara Bracci ◽  
Milena Fini ◽  
Silvia Panzavolta ◽  
Paola Torricelli ◽  
Adriana Bigi
Keyword(s):  
Bone Cement ◽  
Critical Size ◽  
Control Cell ◽  
Critical Size Defects ◽  
Calcium Phosphate Bone Cement ◽  
Proliferation And Differentiation ◽  
In Vivo Tests ◽  
In Vitro Response

We recently developed a new biomimetic calcium phosphate bone cement enriched with gelatin (GEL-CP) which exhibits improved mechanical properties with respect to the control cement (C-CP) and a good response to osteoblast-like cells. In this work, we have extended the investigation to primary culture of osteoblasts derived from normal (N-OB) and osteopenic (O-OB) sheep bones cultured on samples of GEL-CP, and their behavior was compared to that of cells cultured on C-CP as control. Cell morphology, proliferation, and differentiation were evaluated at 3 and 7 days. Preliminary in vivo tests were carried out onto critical size defects in the radius diaphysis of rats.

scholarly journals Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nan Jiang ◽  
Devendra H. Dusane ◽  
Jacob R. Brooks ◽  
Craig P. Delury ◽  
Sean S. Aiken ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Calcium Sulfate ◽  
Bone Graft Substitute ◽  
In Vitro Assays ◽  
In Vivo Experiments ◽  
Orthopaedic Infections ◽  
Clinical Investigations ◽  
Antimicrobial Potency

AbstractThis study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa (PAO1) and S. aureus (SAP231) biofilms in in vitro assays. Antibiotic loaded β-TCP/CS beads were compared with antibiotic loaded beads formed from a well characterized synthetic calcium sulfate (CS) bone void filler. β-TCP/CS antibiotic loaded showed antimicrobial potency against PAO1 in a repeated Kirby-Bauer like zone of inhibition assay for 6 days compared to 8 days for CS. However, both bead types showed potency against SAP231 for 40 days. Both formulations loaded with V + T completely prevented biofilm formation (CFU below detection limits) for the 3 days of the experiment with daily fresh inoculum challenges (P < 0.001). In addition, both antibiotic loaded materials and antibiotic combinations significantly reduced the bioburden of pre-grown biofilms by between 3 and 5 logs (P < 0.001) with V + G performing slightly better against PAO1 than V + T. Our data, combined with previous data on osteogenesis suggest that antibiotic loaded β-TCP/CS may have potential to stimulate osteogenesis through acting as a scaffold as well as simultaneously protecting against biofilm infection. Future in vivo experiments and clinical investigations are warranted to more comprehensively evaluate the use of β-TCP/CS in the management of orthopaedic infections.

Development of Novel Bioactive PMMA-Based Bone Cement and its In Vitro and In Vivo Evaluation

2006 ◽  
Vol 309-311 ◽  
pp. 801-804 ◽  
Author(s):  
S.B. Cho ◽  
Akari Takeuchi ◽  
Ill Yong Kim ◽  
Sang Bae Kim ◽  
Chikara Ohtsuki ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Compressive Strength ◽  
Bone Cement ◽  
The Novel ◽  
In Vivo Evaluation ◽  
Natural Bone ◽  
Pmma Bone Cement ◽  
Rabbit Tibia

In order to overcome the disadvantage of commercialized PMMA bone cement, we have developed novel PMMA-based bone cement(7P3S) reinforced by 30 wt.% of bioactive CaO-SiO2 gel powders to induce the bioactivity as well as to increase mechanical property for the PMMA bone cement. The novel 7P3S bone cement hardened after mixing for about 7 minutes. For in vitro evaluation, apatite forming ability of it was investigated using SBF. When the novel 7P3S bone cement was soaked into SBF, it formed apatite on its surfaces within 1 week Furthermore; there is no decrease in its compressive strength within 9 weeks soaking in SBF. It is though that hardly decrease in compressive strength of 7P3S bone cement in SBF is due to the relative small amount of gel powder or its spherical shape and monosize. In vivo evaluation of the novel 7P3S bone cement was carried out using rabbit. After implantion into rabbit tibia for several periods, the interface between novel bone cement and natural bone was evaluated by CT images. According to the results, the novel bone cement directly contact to the natural bone without fibrous tissue after implantation for 4 weeks. This results indicates that the newly developed 7P3S bone cement can bond to the living bone and also be effectively used as bioactive bone cement without decrease in mechanical property.

In Vivo vs. In Vitro Response Time of Trancutaneous Po2Electrodes A comparison of four devices in newborn infants

1978 ◽  
Vol 22 ◽  
pp. 40-48 ◽  
Author(s):  
H. T. Versmold ◽  
O. Linderkamp ◽  
K. H. Stuffer ◽  
M. Holzmann ◽  
K. P. Riegel
Keyword(s):  
Response Time ◽  
Newborn Infants ◽  
In Vitro Response

The effects of Mn and/or Ni dopants on the in vitro/in vivo performance, structural and magnetic properties of β-tricalcium phosphate bioceramics

2019 ◽  
Vol 45 (17) ◽  
pp. 22752-22758 ◽  
Author(s):  
Tankut Ates ◽  
Sergey V. Dorozhkin ◽  
Omer Kaygili ◽  
Mustafa Kom ◽  
Ismail Ercan ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Tricalcium Phosphate ◽  
Structural And Magnetic Properties

scholarly journals Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan

2020 ◽  
Vol 10 (18) ◽  
pp. 6528 ◽  
Author(s):  
Mayra Eliana Valencia Zapata ◽  
José Herminsul Mina Hernandez ◽  
Carlos David Grande Tovar ◽  
Carlos Humberto Valencia Llano ◽  
Blanca Vázquez-Lasa ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Bone Cement ◽  
Orthopedic Surgery ◽  
Bending Strength ◽  
Orthopedic Implants ◽  
Parietal Bone ◽  
Maximum Temperature ◽  
Cement Interface

Acrylic bone cement (ABC) is one of the most used materials in orthopedic surgery, mainly for the fixation of orthopedic implants to the bone. However, ABCs usually present lack of biological activity and osseointegration capacity that leads to loosening of the prosthesis. This work reports the effect of introducing graphene oxide (GO) and chitosan (CS), separately or together, in the ABC formulation on setting performance, mechanical behavior, and biological properties. Introduction of both CS and GO to the ABC decreased the maximum temperature by 21% and increased the antibacterial activity against Escherichia coli by 87%, while introduction of only CS decreased bending strength by 32%. The results of cell viability and cell adhesion tests showed in vitro biocompatibility. The in vivo response was investigated using both subdermal and bone parietal implantations in Wistar rats. Modified ABCs showed absence of immune response, as confirmed by a normal inflammatory response in Wistar rat subdermal implantation. The results of the parietal bone implantation showed that the addition of CS and GO together allowed a near total healing bone–cement interface, as observed in the micrographic analysis. The overall results support the great potential of the modified ABCs for application in orthopedic surgery mainly in those cases where osseointegration is required.

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