Composition and Antioxidant Activity of the Volatile Oil of Bamboo Leaves from Different Provenances

2011 ◽  
Vol 108 ◽  
pp. 320-325
Author(s):  
Hai Bing Liao ◽  
Chong Jiu Hu ◽  
Ke Yuan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Volatile Oil ◽  
Relative Content ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Total Phenolic ◽  
Bamboo Leaves

The volatile oil of the four different bamboo leaves fromPhyllostachys violascenscv.notata,Sinobambusa tootsik(sieb.) Makino,Sasa subglabraMcClure andPleioblastus kongosanensisf.aureostriaus was obtained by steam distillation; their chemical components were separated and identified by gas chromatography-mass spectrometry (GC-MS). The relative percentage content of the components from the volatile oil was determined by area normalization method. The DPPH free radical scavenging activity (IC50) of the volatile oil was tested by the use of Trolox as a control. The experimental results show that there are 15 kinds of compounds which all simultaneity contained in the volatile oil of four different provenances of bamboo leaves. In four different provenances of volatile oil of bamboo leaves, the relative content of eugenol was obviously higher than other components, were 38.47%, 48.73%, 35.85% and 18.40% respectively. The relative content of the total phenolic were 51.96%, 51.78%, 40.06%, 22.81% respectively. The four different bamboo leaves volatile oil all have the ability of scavenging DPPH free radical and the IC50are 1.16 mg/ml, 1.44 mg/ml, 1.57 mg/ml, 1.71 mg/ml, respectively. The antioxidant activity of the essential oil has a positive correlation with the essential oil's concentration. The result of the experiments can provide reference for further research on the chemical components of different bamboo leaves volatile oil as well as for the exploration and utilization of it.

scholarly journals Comparison of ABTS, DPPH, Phosphomolybdenum Assays for Estimating Antioxidant Activity and Phenolic Compounds in Five Different Plant Extracts

2018 ◽  
Vol 75 (2) ◽  
pp. 110
Author(s):  
Arabela UNTEA ◽  
Alexandra LUPU ◽  
Mihaela SARACILA ◽  
Tatiana PANAITE
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Walnut Leaves

Many plants and their constituents (carotenoids, flavonoids and other phenolic compounds), action to inhibit the free radical by the use of antioxidant activity, helping to a better efficiency of nutrients utilization. The purpose of this study was to investigate the antioxidant activity and phenolic content of five different plants, known for their antioxidant potential. Total phenols were determined by FolinCiocalteumethod, with few modifications. The antioxidant capacity was evaluated by DPPH, ABTS radical-scavenging activity and phosphomolybdenum method. The results showed that the highest antioxidant activity was obtained for walnut leaves in terms of ABTS evaluation and for bilberry leaves by DPPH and phosphomolybdenum methods. In the present study, peppermint, walnut and bilberry leaves proved to have an important antioxidant activity and also a total phenolic content.

scholarly journals Hystrix brachyura Bezoar Characterization, Antioxidant Activity Screening, and Anticancer Activity on Melanoma Cells (A375): A Preliminary Study

Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 39 ◽  
Author(s):  
Al’aina Yuhainis Firus Khan ◽  
Faizah Abdullah Asuhaimi ◽  
Tara K. Jalal ◽  
Fatimah Opeyemi Roheem ◽  
Hatim Abdullah Natto ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Melanoma Cells ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Antioxidant Assays ◽  
Β Carotene

Porcupine bezoars (PBs) are masses of undigested calcareous concretions formed within the gastrointestinal tract. There are undocumented claims that PBs have antioxidant activity and can treat cancers. However, limited scientific study has been carried out to verify these traditional claims. Hence, this study was conducted to characterize the chemical profile and validate the antioxidant and anticancer activity against melanoma cells (A375). PB extract was initially subjected to Fourier-transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GCMS), total phenolic content (TPC), and total flavonoid content (TFC) analyses. The bioautography of antioxidant assays, namely 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazy (DPPH), and β-carotene was performed. An in vitro A375 cell viability assay, apoptosis assay, cell cycle arrest assay, and gene expression assay were carried out as well. The experimental finding revealed 5,10-diethoxy-2,3,7,8-tetrahydro-1H,6H-dipyrrolo[1,2-a:1’,2’-d]pyrazine, ursodeoxycholic acid, and cholest-5-en-3-ol (3 beta)-, carbonochloridate are major compounds detected in PB extract. PB extract has low phenolic content, viz. 698.7 ± 0.93 (µg GAE/5 mg dry weight). The bioautography antioxidant assays revealed a potent antioxidant effect (ABTS > DPPH > β-carotene), with free radical scavenging activity. Furthermore, PB extract exhibited dose- and time-dependent inhibition of cancer activity on A375 cells due to the exhibition of apoptosis via an intrinsic pathway.

scholarly journals Essential Oil Profile, Phenolic Content and Antioxidant Activity of Geranium kikianum

2017 ◽  
Vol 12 (2) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Sanja Ćavar Zeljković ◽  
Kit Tan ◽  
Sonja Siljak-Yakovlev ◽  
Milka Maksimović
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Volatile Oil ◽  
Total Phenolic ◽  
Residual Water ◽  
Oil Composition ◽  
Phytochemical Investigation ◽  
Dpph Method

This work presents the first phytochemical investigation of Geranium kikianum Kit Tan & G. Vold (Geraniaceae), a species endemic to the southern Peloponnese, Greece. The essential oil from aerial parts of the plant was isolated by hydrodistillation and its chemical composition characterized by GC-MS. A total of 26 compounds were identified, constituting 98.3% of the oil composition. Oxygenated sesquiterpenes were the main group of constituents (75.4%). The major component of the oil was the sesquiterpene ketone germacrone (45.6%). Eleven alcohols represented the most diverse chemical class in the volatile oil (36.3%), and phytol was the most abundant acyclic diterpene alcohol (11.4%). The volatile compounds and residual water remaining after hydrodistillation were screened for their radical-scavenging activity by the DPPH method. IC 50 values ranged from 0.2±0.03 mg/mL for the undiluted decoction to 69.7±0.5 mg/mL for the essential oil. The significant antioxidant activity of the residual water, comparable with that of the widespread synthetic antioxidant BHT (0.2±0.01 mg/mL), and almost tenfold higher than thymol (1.90 ± 0.04 mg/mL), correlates with a high content of total phenolic compounds (100.2±1.7 mg GAE/g), and flavonoids (11.9±1.2 mg GAE/g).

Total Phenolic Content and Free Radical Scavenging Activity of Nelumbo nucifera Gaertn.

2019 ◽  
Vol 886 ◽  
pp. 52-55
Author(s):  
Khemjira Jarmkom ◽  
Nakuntwalai Wisidsri ◽  
Pattaranut Eakwaropas ◽  
Warachate Khobjai
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Significant Difference

All parts of N. nucifera are used as oriental medicine for various medicinal purposes. The aimed of this study was to determine phenolic compound and antioxidant activity of different parts of three lotus (N. nucifera including of Roseum Plenum (RP), Album Plenum (AP), and Hindu Lotus (HL)). Total phenolic content using Folin-Ciocalteu reagent method and free radical scavenging activities using DPPH assay were determined. Three deferent parts of lotus (leaves, stem, and flower) were determined. The result indicated that Roseum Plenum leaf showed the highest total phenolic contents and Roseum Plenum flower showed the highest antioxidant activities. Total phenolic content of different part of different of N. nucifera showed a significant difference (p < 0.05). It also was not uniform when compare between different of N. Nucifera. The results found that the total phenolic content was the opposite of antioxidant activity. This result may be attributed to antioxidant activity may not be from phenolic content. Therefore, it is important to research and develop the potential of lotus extraction in the future.

scholarly journals Purification and Characterization of Phenolic Antioxidant from Corncob Liquid Smoke

2020 ◽  
Vol 32 (12) ◽  
pp. 2985-2990
Author(s):  
Edi Suryanto ◽  
Mercy R.I. Taroreh ◽  
Lydia Irma Momuat
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Fractional Distillation ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Simple Distillation ◽  
Liquid Smoke ◽  
Total Antioxidant

This study aimed to purify and characterize the antioxidant activity of liquid smoke acquired from corncobs. The smoke of crude liquids was generated through pyrolysis and was purified through fractional and simple distillation. The liquid smoke was analyzed using crude fibres, cellulose, hemicellulose, lignin, antioxidant activity and total phenolic compounds. Gas chromatography-mass spectrometry (GC-MS) was employed to analyze the volatile compounds of liquid smoke. Fractional distillation exhibited the higher carbonyl and phenol content than simple distillation, and the overall acid content of fractional distillation was relatively lower. Moreover, the liquid smoke obtained from fractional distillation exhibited the higher total antioxidant and free radical scavenging activity than simple distillation. The GC-MS results showed different derivatives and phenolic compounds as the most dominant components of the liquid smoke of corncobs. The major antioxidant components of fractional distillation include 2-methoxyphenol, phenol, 2,5-dimethylphenol, 2-methylphenol, 2-methoxy-4-methylphenol, 4-ethyl-2-methoxyphenol and 4-ethylphenol, and those of simple distillation include phenol, 2-methylphenol, 2-methoxy- phenol, 2,6-dimethoxyphenol and 3-ethylphenol.

scholarly journals Antioxidant activity of three different Serbian floral honeys

2011 ◽  
pp. 145-155 ◽  
Author(s):  
Sladjana Savatovic ◽  
Dejan Dimitrijevic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet ◽  
Gordana Cetkovic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Content

In this study, three Serbian honey samples (Acacia, Linden and "Homoljski med") were analyzed to determine their total phenolic, flavonoid and antioxidant content, as well as their in vitro antioxidant activity. The antioxidant activity of honeys was examined by different tests, including the reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. In addition, correlations between the antioxidant activity and total phenolic, as well as flavonoid content were also sought. The highest content of total phenolics (27.44 mg/100 g), flavonoids (9.78 mg/100 g), reducing power and DPPH free radical scavenging activity were obtained in the case of Linden honey. The EC50 values of the Linden honey, determined based on the reducing power and DPPH radical scavenging activity, were 24.17 mg/ml and 51.34 mg/ml, respectively. Also, the antioxidant content was highest for Linden honey, and it valued 5.45 mg QEAC/100 g (expressed as mg of quercetin equivalent antioxidant content - QEAC per 100 g of honey) and 7.82 mg AEAC/100 g (expressed as mg of ascorbic acid equivalent antioxidant content - AEAC per 100 g of honey). Also, a linear correlation was observed between the antioxidant activity and total phenolics, as well as total flavonoids.

scholarly journals Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris

2021 ◽  
Author(s):  
Aderaw Anteneh Belew ◽  
Getachew G/Mariam W/Hana ◽  
Desta Shumuye Meshesha ◽  
Mulugeta Legese Akele
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Gallic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Phenolic Contents

Abstract Background: Rhus vulgaris is rich in various classes of polyphenols and flavonoids that act as free radical scavengers and reduce oxidative stress and cure various harmful human diseases. The plant is a traditionally known medicinal plant which is used against a number of diseases including cancer. Methods: The current investigation points towards the investigating quantitative phenolic contents, flavonoid contents and the free radical scavenging activity & antioxidant activity of Rhus vulgaris extract in n-hexane, acetone, and 80% of aqueous methanol. The phenolic contents were estimated by Folin–Ciocalteu procedure and gallic acid as a reference molecule whereas the total flavonoid contents were determined by using aluminum chloride and catechin. In addition, the dilution serial method was used to evaluate the leaves extract of rhus vulgaris, and the DPPH (2,2-diphenyl-1-picrylhydrazyl) method was utilized to assess the above-mentioned extracts against oxidative stress. Results: The result revealed that the ranges of total phenolic content from 5.82 ± 4.6 to 83.15 ± 7.6 mg GAE/g of the dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentrations were varied from 2.21 ± 7.34to 23.47 ± 4.87 mg CE/g, expressed as catechin equivalents. Antioxidant activity of extracts was expressed as the concentration of DPPH radical’s inhibition ranges from 1.2 ± 0.32 to 22.86 ± 3.71 mg AAE/ g. Conclusion: The 80% aqueous methanolic extract of Rhus vulgaris showed the highest phenolic and flavonoid contents and strong antioxidant potential and it could be used as antibiotics for different curable and incurable diseases.

scholarly journals Brown beer vinegar: A potentially functional product based on its phenolic profile and antioxidant activity

2018 ◽  
Vol 83 (1) ◽  
pp. 19-30 ◽  
Author(s):  
Elena Mudura ◽  
Teodora Coldea ◽  
Carmen Socaciu ◽  
Floricuţa Ranga ◽  
Carmen Pop ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Acetic Fermentation ◽  
Functional Product

The aim of the present study was to create a functional, enriched in polyphenols and free of alcohol product obtained by acetic fermentation of beer. Beer and vinegar were tested first for their phenolic content and antioxidant activity, by the Folin Ciocalteu and the free radical scavenging activity by the 1,1-diphenyl-2-picrylhydrazyl free-radical scavenging assay, respectively. Then, the separation and identification of the 30 phenolic compounds was realized by high-performance liquid chromatography coupled with positive electrospray ionisation and diode array detection (HPLC-DAD?ESI(+)-MS) analysis. Identification of the phenolic compounds data was realized based on the UV spectra of each compound. Based on a calibration curve (R2 = 0.9985), the amounts of the phenolic compounds, expressed as mg cathechin equivalents (CE)/L, were calculated. The total phenolic content of the beer and vinegar samples determined using Folin?Ciocalteu reagent were of 428.9?1.58 and 661.5?7.69 mg GAE L-1, respectively, which contributed to the high antioxidant activity in the vinegar sample of 82.18 %. Statistically significant differences were observed after acetic fermentation between each parameter (p < 0.05). Brown beer vinegar represents a rich source of polyphenols and phenolic derivatives, compared to beer. By its increased phenolic content and antioxidant activity, brown beer vinegar could be considered another source of valuable compounds to beer, which could also be of interest in special diets.

scholarly journals Karakterisasi dan Aktivitas Antioksidan Tepung Sagu Baruk (Arenga microcarpha)

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 125
Author(s):  
Elisa Putri Tarigan ◽  
Lidya Irma Momuat ◽  
Edi Suryanto
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Chemical Characteristics ◽  
Scavenging Activity ◽  
Phytochemical Content ◽  
Phenolic And Flavonoid

Tujuan penelitian ini adalah untuk mempelajari karakteristik kimia tepung sagu baruk yang mengandung senyawa fitokimia dan mempelajari aktivitas antioksidan dari ekstrak tepung sagu baruk. Tepung sagu baruk diekstrak dengan pelarut etanol 50% dengan cara maserasi selama 24 jam. Setelah itu, tepung sagu diuji  proksimat, ekstrak dianalisis kandungan fitokimia fenolik, flavonoid dan tanin terkondensasi. Pengujian aktivitas antioksidan menggunakan radikal bebas DPPH dan daya reduksi dengan spektrofotometer UV/Vis. Hasil penelitian menunjukkan pada pengujian proksimat bahwa tepung tepung sagu baruk memenuhi syarat Standar Nasional Indonesia, tetapi berbeda dengan kadar serat kasar. Total kandungan fenolik dan flavonoid  menunjukkan bahwa tepung sagu akuades (SA) lebih tinggi daripada tepung sagu komersial (TSBK). Hasil radikal bebas sagu tepung sagu SA lebih tinggi dari TSBK sama halnya dengan kemampuan daya reduksi SA jauh lebih tinggi dari TSBK. Hasil penelitian ini menyimpulkan bahwa tepung sagu baruk memiliki kandungan fitokimia fenolik dan flavonoid yang dapat berpotensi sebagai antioksidan.Objectives of this research were to study the chemical characteristics of sago baruk flour containing phytochemicals and antioxidant activity of sago baruk flour extract. Sago baruk flour was extracted with 50% ethanol by maceration for 24 hours. Afterward, sago flour was tested for proximate and the extract was analyzed for phenolic, flavonoids, and condensed tanin. DPPH free radical was used to examine antioxidant activity and spectrophotometry method was used to determine the reduction ability. The result for proximate test showed that sago flour extract meets Indonesian Nasional Standard requirements. Total phenolic and flavonoid contents in the flour extracted with aquadest (SA) were higher than that in commercial flour (TSBK). Free radical scavenging activity of sago flour SA was higher than that of TSBK, as well as the reduction ability of SA that was much higher than of TSBK. It was concluded that sago baruk flour possess phytochemical content of phenolic and flavonoid which were potential as antioxidant.

Evaluation of antioxidant and Anthelmintic activity of methanolic flower extract of Nyctanthes arbor-tristis

2018 ◽  
Vol 9 (1) ◽  
pp. 78
Author(s):  
Vijaya Jyothi M ◽  
Bhargav E ◽  
Pavan Kumar K ◽  
Praneeth Gowd K ◽  
Ram Pavan S
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Iridoid Glycosides ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Total Phenolic ◽  
Standard Drug ◽  
Flower Extract ◽  
Reducing Ability ◽  
Total Flavonoids Content

Nyctanthes arbour-tristis is a shrub belongs to the family Oleaceae. The flowers of this plant are fragrant since the presence of flavonol glycosides. It has also been reported for the presence of β-sitosterol, iridoid glycosides, tannins etc., and known to have immunostimulant, hepatoprotective, antiviral and antifungal activities. In the present study an attempt is made to identify antioxidant capacity and anthelminthic potential of methanolic flower extract of Nyctanthes arbour-tristis. Antioxidant activity was evaluated by total phenolic content assay, total flavonoids content assay, free radical scavenging activity and reducing ability methods. Anthelmintic activity was evaluated on Perithima posthuma using Piperazine citrate as standard drug. The results obtained for the above activities reveals that Nyctanthes arbour-tristis shows considerable antioxidant activity for all the methods and anthelminthic potential at 300 mg/ml. Keywords: arbour-tristis; antioxidant activity; anthelminthic activity; Perithima posthuma; Piperazine citrate.

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