Chip-Based Portable Device for Detection of Microalgae Viability in Ballast Water

2014 ◽  
Vol 522-524 ◽  
pp. 3-6
Author(s):  
Jun Sheng Wang ◽  
Hui Chu ◽  
Jin Yang Sun ◽  
You Nan Song ◽  
Jin Hu Jiang ◽  
...  
Keyword(s):  
Ballast Water ◽  
Rapid Detection ◽  
Detection System ◽  
Pulse Signal ◽  
Label Free ◽  
Environmental Threats ◽  
Sheath Fluid ◽  
Micro Channels ◽  
Drive Systems ◽  
Ship Ballast Water

Ship ballast water has been identified as one of four major environmental threats by the international maritime organization. This paper presents a portable on-site rapid detection system of microalgae in ship ballast water. The system consists of a microfluidic chip platform, LED light source and drive systems, optical detection system, ARM software and hardware system, power supply system and so on. Microalgae particles can go through the detection area inside the micro channels one by one by using a sheath fluid focus. The resulting chlorophyll fluorescence is converted to the electrical pulse signal by photodiode. The experimental results show that the system can quickly and accurately detect the ballast water living microalgae concentration. The minimum detectable microalgae size is about 3μm (mean diameter). This system has some advantages such as miniaturized, portable, fast, accurate and label free, and has great potential for on-site rapid detection of ship ballast water.

Rapid detection and quantitation of hepatitis B virus DNA by real-time PCR using a new fluorescent (FRET) detection system

2004 ◽  
Vol 30 (2) ◽  
pp. 191-195 ◽  
Author(s):  
Sani Hussein Aliyu ◽  
Muktar Hassan Aliyu ◽  
Hamisu M Salihu ◽  
Surendra Parmar ◽  
Hamid Jalal ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Detection System ◽  
Hepatitis B Virus Dna ◽  
B Virus

scholarly journals Design of Inverted Nano-Cone Arrayed SERS Substrate for Rapid Detection of Pathogens

2021 ◽  
Vol 11 (17) ◽  
pp. 8067
Author(s):  
Zixun Jia ◽  
Sarah Asiri ◽  
Asma Elsharif ◽  
Widyan Alamoudi ◽  
Ebtesam Al-Suhaimi ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Rapid Detection ◽  
Close Contact ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Substrate ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Order Of Magnitude ◽  
The Right ◽  
Pathogen Diagnosis

Rapid detection of bacteria is a very critical and important part of infectious disease treatment. Sepsis kills more than 25 percent of its victims, resulting in as many as half of all deaths in hospitals before identifying the pathogen for patients to get the right treatment. Raman spectroscopy is a promising candidate in pathogen diagnosis given its fast and label-free nature, only if the concentration of the pathogen is high enough to provide reasonable sensitivity. This work reports a new design of surface-enhanced Raman spectroscopy (SERS) substrate which will provide high enough sensitivity and fast and close contact of the target structure to the optical hot spots for immunomagnetic capturing-based bacteria-concentrating technique. The substrate uses inverted nanocone structure arrays made of transparent PDMS (Polydimethylsiloxane) to funnel the light from the bottom to the top of the cones where plasmonic gold nanorods are located. A high reflective and low loss layer is deposited on the outer surface of the cone. Given the geometry of cones, photons are multi-reflected by the outer layer and thus the number density of photons at hotspots increases by an order of magnitude, which could be high enough to detect immunomagnetically densified bacteria.

A novel label‐free chronoamperometric immunosensor based on a biocomposite material for rapid detection of carcinoembryonic antigen

2022 ◽  
Author(s):  
Chochanon Moonla ◽  
Suphaporn Chenkhuruthum ◽  
Tik Ouiram ◽  
Anchana Preechaworapun ◽  
Weerinradah Tapala ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Rapid Detection ◽  
Label Free ◽  
Biocomposite Material

Highly Sensitive, Selective, and Rapid Detection of miRNA-21 using an RCA/G-Quadruplex/QnDESA Probing System

2021 ◽  
Author(s):  
Young Jun Seo ◽  
Tasnima Alam Asa ◽  
Ravi Kumara Guralamatta Siddappa
Keyword(s):  
Rapid Detection ◽  
Detection System ◽  
Single Step ◽  
Highly Sensitive ◽  
G Quadruplex

In this study we developed a very simple and rapid miRNA 21 detection system using a novel quinolinium diethylamino salicylaldehyde (QnDESA) probe for sensing the 22AG hybrid G-quadruplex with single-step...

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