Protein Biomarkers from Physiological Disorder Syndrome in Leave, Peel and Seed of Longan on Fruit Growth Using One-Dimension Electrophoresis Technique Followed by Protein Identification with LC-MS/MS

2019 ◽  
Vol 886 ◽  
pp. 14-20
Author(s):  
Ekawit Threenet ◽  
Achara Kleawkla ◽  
Yossaporn Kaewkalong ◽  
Winai Wiriyaalongkorn ◽  
Adisak Joomwong ◽  
...  
Keyword(s):  
Storage Protein ◽  
Protein Identification ◽  
Recovery Process ◽  
Fruit Growth ◽  
Protein Biomarkers ◽  
Vegetative Storage Protein ◽  
Physiological Disorder ◽  
Advanced Technique ◽  
Bisphosphate Carboxylase ◽  
Before And After

Proteomic investigation of leaves, peels and seeds on longan (Dimocarpus longan Lour. cv. Daw) at Maejo University’s farm in Chiang Mai province, Thailand. There were comparisons between normal and physiological disorder syndromes in longan on fruit growth (5, 10, 15, 20, 25 and 30 weeks fruition, respectively) by one dimensional electrophoresis (1-D gel) technique at a concentration of 12.5% gel in reducing condition with Coomassie brilliant blue R-250 staining coupling on liquid chromatography tandem mass spectrometry (LC-MS/MS). The research showed that vegetative storage protein (25.2 KDa), a storage protein in plants, was expressed in leaves, peels and seeds on before and after physiological disorder syndrome occurring. A protein involved on photosynthesis, the ribulose-1,5-bisphosphate carboxylase/oxygenase (48.3 KDa), was represented only on leaves at 30 weeks after fruition but not in peels and seeds of longan. Two proteins, the BnaC01g20210 (35.8 KDa) and predicted protein (40.11 KDa), which could not be identified the type and function of the metabolism, were decreased in diseased longan. These proteins may be important protein in part of the recovery process of abnormal longan. Therefore, advanced technique will be used for further proteomic studies.

Breaking The Silos: an online and narrator-led role-playing game for multi-risk DRR management

2021 ◽  
Author(s):  
Marleen de Ruiter ◽  
Anaïs Couasnon ◽  
Philip Ward
Keyword(s):  
Expert Knowledge ◽  
Recovery Process ◽  
Role Playing ◽  
Disaster Risk ◽  
Decision Makers ◽  
Economic Sectors ◽  
Disaster Impacts ◽  
Strong Focus ◽  
Before And After ◽  
Role Playing Game

<p>The increased complexity of disaster risk due to climate change, expected population growth and the increasing interconnectedness of disaster impacts across communities and economic sectors, require Disaster Risk Reduction (DRR) measures and practitioners that are better able to address these complexities. Nonetheless, in the traditional risk paradigm, there is a strong focus on single-hazards and the risk faced by individual communities and sectors.</p><p><em>Breaking the Silos</em> is a narrator-led, role-playing game designed to support decision makers and stakeholders in understanding and managing the complexities of implementing DRR measures in a multi-(hazard) risk setting. The game starts in de aftermath of a (randomly selected) disaster. The different roles include key decision-makers and stakeholders of a country. The team is responsible for the post-disaster recovery process and can decide to implement DRR measures. However, while some of these DRR measures can decrease risk of one hazard, they can increase the risk of another hazard. In each subsequent round, the team faces another (randomly selected) disaster. Unlike many other risk serious games, <em>Breaking the Silos </em>includes many random factors to better simulate reality. The roles are designed such that expert knowledge and objectives are spread throughout the participants and they can even be conflicting at times.</p><p>The game was successfully launched during the World Bank’s 2020 Understanding Risk conference. Before and after playing the game, participants were asked to complete surveys asking them about their perception of the challenges of Disaster Risk Management and whether the game raised their awareness of these challenges. The preliminary findings indicate that <em>Breaking the Silos</em> is a useful tool in supporting decision makers and practitioners to become aware of (the risks of) hazard-silo thinking and possible (a)synergies of DRR measures.</p>

scholarly journals Changes in Pore Structure of Coal Caused by CS2 Treatment and Its Methane Adsorption Response

Geofluids ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Run Chen ◽  
Yong Qin ◽  
Pengfei Zhang ◽  
Youyang Wang
Keyword(s):  
Pore Structure ◽  
Adsorption Capacity ◽  
Gas Adsorption ◽  
Recovery Process ◽  
Micropore Volume ◽  
Methane Adsorption ◽  
Coal Bed ◽  
Methane Recovery ◽  
Before And After ◽  
Key Issues

The pore structure and gas adsorption are two key issues that affect the coal bed methane recovery process significantly. To change pore structure and gas adsorption, 5 coals with different ranks were treated by CS2 for 3 h using a Soxhlet extractor under ultrasonic oscillation conditions; the evolutions of pore structure and methane adsorption were examined using a high-pressure mercury intrusion porosimeter (MIP) with an AutoPore IV 9310 series mercury instrument. The results show that the cumulative pore volume and specific surface area (SSA) were increased after CS2 treatment, and the incremental micropore volume and SSA were increased and decreased before and after Ro,max=1.3%, respectively; the incremental big pore (greater than 10 nm in diameter) volumes were increased and SSA was decreased for all coals, and pore connectivity was improved. Methane adsorption capacity on coal before and after Ro,max=1.3% also was increased and decreased, respectively. There is a positive correlation between the changes in the micropore SSA and the Langmuir volume. It confirms that the changes in pore structure and methane adsorption capacity due to CS2 treatment are controlled by the rank, and the change in methane adsorption is impacted by the change of micropore SSA and suggests that the changes in pore structure are better for gas migration; the alteration in methane adsorption capacity is worse and better for methane recovery before and after Ro,max=1.3%. A conceptual mechanism of pore structure is proposed to explain methane adsorption capacity on CS2 treated coal around the Ro,max=1.3%.

Who We Are

2020 ◽  
pp. 26-46
Author(s):  
Kevin M. Fitzpatrick ◽  
Matthew L. Spialek
Keyword(s):  
Data Collection ◽  
Recovery Process ◽  
Methodological Framework ◽  
Online Surveys ◽  
Specific Data ◽  
Face To Face ◽  
Secondary Sources ◽  
Recovery Processes ◽  
Before And After

Chapter two describes the methodological framework and design for this project. The authors present a discussion of the methods used to select persons for both face-to-face interviews and online surveys, along with the follow-up strategies used to talk with civilians and organizational officials involved in the recovery process. This chapter discusses both the approach to the data collection, as well as what specific data the authors were interested in acquiring as it pertained to understanding how displacement and recovery processes varied across individual survivors. Finally, the chapter discusses in detail the numerous strategies employed to tell the survivors’ stories—pictures, maps, tables, charts, and narratives, along with additional data from secondary sources to help characterize the places where survivors were living both before and after the disaster.

Protein identification before and after glyphosate exposure in Lolium multiflorum genotypes

2018 ◽  
Vol 74 (5) ◽  
pp. 1125-1133 ◽  
Author(s):  
Arthur AM Barroso ◽  
Marilia G de S Costa ◽  
Nelson J Neto ◽  
Juciléia I dos Santos ◽  
Tiago S Balbuena ◽  
...  
Keyword(s):  
Protein Identification ◽  
Lolium Multiflorum ◽  
Before And After

Vegetative storage protein expression during terminal bud formation in poplar

2001 ◽  
Vol 31 (6) ◽  
pp. 1098-1103 ◽  
Author(s):  
Susan D Lawrence ◽  
Janice EK Cooke ◽  
John S Greenwood ◽  
Theresa E Korhnak ◽  
John M Davis
Keyword(s):  
Protein Expression ◽  
Shoot Apex ◽  
Storage Protein ◽  
Messenger Rna ◽  
Storage Proteins ◽  
N Cycling ◽  
Mrna Levels ◽  
Vegetative Storage Protein ◽  
Bud Formation ◽  
Terminal Bud

Trees recycle nitrogen (N) to conserve this valuable nutrient. The processes that regulate N recycling within trees are poorly understood at the molecular level. Because vegetative storage proteins (VSPs) are thought to play important roles in within-plant N cycling, we are investigating the expression of VSP genes to gain insights into how seasonally controlled N cycling is regulated in trees. We compared steady-state mRNA levels of three different VSP homologs during short day induced terminal bud formation in hybrid poplar (Populus trichocarpa Torr. & Gray × Populus deltoides Bartr. ex Marsh.) – WIN4 (wound-inducible protein 4), BSP (bark storage protein), and pni288 (poplar nitrogen-regulated cDNA 288, a newly identified sequence). We determined that win4 and pni288 transcripts decrease, while bsp transcripts increase, as the terminal bud is formed. Immunolocalization analysis indicated that, during apical bud formation, BSP accumulates in the ground meristem and in parenchyma cells adjacent to xylem and proximal to the apical dome. Based on messenger RNA and protein expression analysis, we conclude that different VSPs play distinct roles in the poplar shoot apex, with BSP accumulating as a reserve near the shoot apex during terminal bud formation.

scholarly journals Vegetative Storage Protein in Litchi chinensis, a Subtropical Evergreen Fruit Tree, Possesses Trypsin Inhibitor Activity

2007 ◽  
Vol 100 (6) ◽  
pp. 1199-1208 ◽  
Author(s):  
Wei-Min Tian ◽  
Shi-Qing Peng ◽  
Xu-Chu Wang ◽  
Min-Jing Shi ◽  
Yue-Yi Chen ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Storage Protein ◽  
Fruit Tree ◽  
Trypsin Inhibitor Activity ◽  
Vegetative Storage Protein ◽  
Inhibitor Activity ◽  
Litchi Chinensis

scholarly journals SENSITIVITY OF RAT BRAIN SENSORIMOTOR CORTEX NEURONS TO MEDIATORS IN HEMORRHAGIC STROKE

2019 ◽  
Vol 16 (33) ◽  
pp. 749-755
Author(s):  
A. MESHCHERYAKOV ◽  
E. KOPLIK ◽  
V. VOLKOV ◽  
H. IONKINA ◽  
M. BUDNIKOV ◽  
...  
Keyword(s):  
Sensorimotor Cortex ◽  
Hemorrhagic Stroke ◽  
Acute Stress ◽  
Recovery Process ◽  
Individual Characteristics ◽  
Stress Disorders ◽  
Stressful Situation ◽  
Before And After ◽  
Stress Influences ◽  
Stress Influence

The problem of post-stress disorders of cerebral circulation, the most severe of which is cerebral hemorrhage, or hemorrhagic stroke, is extremely urgent. Concerning the survival rate, previous studies have shown that in the same type of conflict situations, individuals resistant and predisposed to emotional stressful influences are clearly distinguished. However, the neural mechanisms providing neuromediatory regulation of responses to stressful effects are still little studied. The analysis of neurochemical sensitivity to acetylcholine and norepinephrine of the sensorimotor cortex neurons of rats having different activity according to behavior parameters, i.e., resistance to emotional stress influences before and after experimental hemorrhagic stroke (EHS) was carried out. The recovery process after an EHS due to neural activity in prognostically stable (VA) and predisposed (NA) to stressful situation rats has individual characteristics. Acute stress influence before EHS development changes the nature of neurons activity of the sensorimotor cortex in VA and NA rats on the third and seventh day after EHS. EHS leads to specific changes in the sensitivity of neurons to acetylcholine and norepinephrine in VA and NA rats. Acetylcholine microionophoresis causes activation of the neuron impulse activity in VA and NA rats, and these changes are the same after EHS. Acute stress influence before EHS development does not change nature of neuron responses to norepinephrine microionophoresis in VA animals. On the contrary, in NA rats loss of neuron sensitivity to norepinephrine during EHS development against stress was noted. It is possible that acute stress influence before EHS development can change brain noradrenergic system activity in NA rats, which are prognostically predisposed to stress influences. The data obtained stress the plasticity of the central nervous structure in development of response to stressful influences. (The ethics commission approval protocol number is 18-15 from 13th of Marth 2018.)

Sign in / Sign up

Export Citation Format

Share Document