Cytotoxicity Assessment of SH-SY5Y Cells Grown on Graphene Sheet
2015 ◽
Vol 1110
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pp. 311-314
Keyword(s):
We investigate the effect of cell culture conditions, using pristine graphene sheets as growth substrate, on the human nerve cell line (SH-SY5Y). In order to evaluate cell viability and morphology, we applied the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, and fluorescence microscopy of cells stained with Hochest 33342 and Calcein AM. Human nerve cells exhibited 84% viability on pristine graphene sheets compared with control (cell culture polystyrene) after 3 days culturing. Fluorescence data showed that the presence of graphene did not influence cell morphology. These results suggest that graphene sheets may be used for biological applications.
2021 ◽
Vol 1885
(3)
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pp. 032004
2003 ◽
Vol 92
(10)
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pp. 1957-1967
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1989 ◽
Vol 25
(9)
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pp. 806-812
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Keyword(s):
2013 ◽
Vol 405
(29)
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pp. 9365-9374
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2017 ◽
Vol 158
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pp. 488-497
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Keyword(s):
Keyword(s):
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2003 ◽
Vol 16
(8)
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pp. 1102-1107
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Keyword(s):
1991 ◽
Vol 17
(4)
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pp. 287-296
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