Reductive Leaching of Jarosites by Shewanella putrefaciens - Influence of Humic Substances and Chelators in Mineral Dissolution

The anaerobic bioreduction of three Fe (III) ores by a type strain of Shewanella putrefaciens has been investigated. The release of ferrous ion indicated the microbial reduction of jarosite and promotes the subsequent secondary mineralization, leading to the formation of various iron-nearing minerals. In addition, the influence of citrate and EDTA in the medium acting as chelating agents, and an electron shuttling molecule were studied. While the citrate and humic substances increased the iron reduction rate, AQDS inhibit the mineral bioreduction and dissolution. S. putrefaciens do not have the necessity of the direct contact between cells and jarosites and, in consequence, cell attachment and biofilm formation on the mineral surface is scant.

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Facet-dependent reductive dissolution of hematite nanoparticles by Shewanella putrefaciens CN-32

Environmental Science Nano ◽

10.1039/d0en00555j ◽

2020 ◽

Vol 7 (9) ◽

pp. 2522-2531 ◽

Cited By ~ 1

Author(s):

Shiwen Hu ◽

Yundang Wu ◽

Zecong Ding ◽

Zhenqing Shi ◽

Fangbai Li ◽

...

Keyword(s):

Redox Potential ◽

Iron Reduction ◽

Reduction Rate ◽

Shewanella Putrefaciens ◽

Reductive Dissolution ◽

Hematite Nanoparticles ◽

Adsorption Sites ◽

Dissimilatory Iron Reduction

The hematite {001} possessed more adsorption sites, more positive redox potential, and higher conductivity than {100}, resulting that the dissimilatory iron reduction rate of hematite {001} by S. putrefaciens CN-32 was easier than that of {100}.

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Enhancement of iron toxicity in L929 cells by d-glucose: accelerated(re-)reduction

Biochemical Journal ◽

10.1042/bj20020639 ◽

2002 ◽

Vol 368 (2) ◽

pp. 517-526 ◽

Cited By ~ 21

Author(s):

Ilka LEHNEN-BEYEL ◽

Herbert de GROOT ◽

Ursula RAUEN

Keyword(s):

Ethyl Ester ◽

Iron Reduction ◽

Cell Damage ◽

Reduction Rate ◽

Fold Increase ◽

Iron Complex ◽

Iron Toxicity ◽

Intracellular Iron ◽

Iron Valence ◽

Iron Pool

It has recently been shown that an increase in the cellular chelatable iron pool is sufficient to cause cell damage. To further characterize this kind of injury, we artificially enhanced the chelatable iron pool in L929 mouse fibroblasts using the highly membrane-permeable complex Fe(III)/8-hydroxyquinoline. This iron complex induced a significant oxygen-dependent loss of viability during an incubation period of 5h. Surprisingly, the addition of d-glucose strongly enhanced this toxicity whereas no such effect was exerted by l-glucose and 2-deoxyglucose. The assumption that this increase in toxicity might be due to an enhanced availability of reducing equivalents formed during the metabolism of d-glucose was supported by NAD(P)H measurements which showed a 1.5—2-fold increase in the cellular NAD(P)H content upon addition of d-glucose. To assess the influence of this enhanced cellular reducing capacity on iron valence we established a new method to measure the reduction rate of iron based on the fluorescent iron(II) indicator PhenGreen SK. We could show that the rate of intracellular iron reduction was more than doubled in the presence of d-glucose. A similar acceleration was achieved by adding the reducing agents ascorbate and glutathione (the latter as membrane-permeable ethyl ester). Glutathione ethyl ester, as well as the thiol reagent N-acetylcysteine, also caused a toxicity increase comparable with d-glucose. These results suggest an enhancement of iron toxicity by d-glucose via an accelerated (re-)reduction of iron with NAD(P)H serving as central electron provider and ascorbate, glutathione or possibly NAD(P)H itself as final reducing agent.

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The involvement of lectins and lectin-like humic substances in biofilm formation on RO membranes - is TEP important?

Desalination ◽

10.1016/j.desal.2016.07.036 ◽

2016 ◽

Vol 399 ◽

pp. 61-68 ◽

Cited By ~ 10

Author(s):

Harvey Winters ◽

T.H. Chong ◽

Anthony G. Fane ◽

William Krantz ◽

Miles Rzechowicz ◽

...

Keyword(s):

Humic Substances ◽

Biofilm Formation

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DNA relatedness of oil-field isolates of Shewanella putrefaciens

Canadian Journal of Microbiology ◽

10.1139/m89-153 ◽

1989 ◽

Vol 35 (10) ◽

pp. 925-931 ◽

Cited By ~ 29

Author(s):

Kathleen M. Semple ◽

James L. Doran ◽

D. W. S. Westlake

Keyword(s):

Iron Reduction ◽

Shewanella Putrefaciens ◽

Oil Field ◽

The Other ◽

Nucleic Acid Hybridization ◽

Dna Relatedness ◽

Field Isolates ◽

Shewanella Species ◽

Sulfide Production

Classification of several oil-field isolates of Shewanella putrefaciens was assessed by nucleic acid hybridization techniques. The results of DNA – DNA hybridization analysis generally confirmed the phenetic characterization of these isolates and supported the classification of oil-field isolates of S. putrefaciens groups 1, 3, and 4. However, two group 2 isolates were considered to be mistakenly classified. Strain ESSO 1-1 appeared to belong to group 3, a result which was supported by the pattern of 5S rRNA hybridization to restriction digests of genomic DNA, and strain 213 appeared to be a member of group 1. Several of the oil-field strains of S. putrefaciens were found to possess indigenous plasmids, a feature which was not shared by the other strains of S. putrefaciens examined. This study indicates mat these oil-field isolates were more closely related to strains of S. putrefaciens isolated from various environments than to the other Shewanella species (S. benthica and S. hanedai) that shared the important metabolic characteristics of iron reduction and sulfide production from thiosulfate. There was very little genetic relationship found between Shewanella spp. and the other species studied.Key words: Shewanella putrefaciens, DNA relatedness, oil-field iron reduction, sulfide production, taxonomy.

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Sulfur-mediated electron shuttling during bacterial iron reduction

Science ◽

10.1126/science.1252066 ◽

2014 ◽

Vol 344 (6187) ◽

pp. 1039-1042 ◽

Cited By ~ 94

Author(s):

T. M. Flynn ◽

E. J. O'Loughlin ◽

B. Mishra ◽

T. J. DiChristina ◽

K. M. Kemner

Keyword(s):

Iron Reduction ◽

Electron Shuttling

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Periodicity of Cell Attachment Patterns during Escherichia coli Biofilm Development

Journal of Bacteriology ◽

10.1128/jb.185.18.5632-5638.2003 ◽

2003 ◽

Vol 185 (18) ◽

pp. 5632-5638 ◽

Cited By ~ 24

Author(s):

Konstantin Agladze ◽

Debra Jackson ◽

Tony Romeo

Keyword(s):

Escherichia Coli ◽

Laminar Flow ◽

Biofilm Formation ◽

Cell Attachment ◽

Bacterial Biofilms ◽

Flow Conditions ◽

Biofilm Development ◽

Complex Architecture ◽

Activator Inhibitor ◽

Laminar Flow Conditions

ABSTRACT The complex architecture of bacterial biofilms inevitably raises the question of their design. Microstructure of developing Escherichia coli biofilms was analyzed under static and laminar flow conditions. Cell attachment during early biofilm formation exhibited periodic density patterns that persisted during development. Several models for the origination of biofilm microstructure are considered, including an activator-inhibitor or Turing model.

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Microbial Reduction of Lepidocrocite γ-FeOOH by Shewanella putrefaciens; The Formation of Green Rust

Industrial Applications of the Mössbauer Effect ◽

10.1007/978-94-010-0299-8_24 ◽

2002 ◽

pp. 231-237

Author(s):

G. Ona-Nguema ◽

M. Abdelmoula ◽

F. Jorand ◽

O. Benali ◽

A. Géhin ◽

...

Keyword(s):

Shewanella Putrefaciens ◽

Microbial Reduction ◽

Green Rust

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Effects of Carbon Addition on Dissimilatory Fe(III) Reduction in Freshwater Marsh and Meadow Wetlands

Sustainability ◽

10.3390/su10114309 ◽

2018 ◽

Vol 10 (11) ◽

pp. 4309 ◽

Cited By ~ 1

Author(s):

Xiaoyan Zhu ◽

Yuxiang Yuan ◽

Ming Jiang

Keyword(s):

Iron Reduction ◽

Reduction Rate ◽

Negative Relationship ◽

Sanjiang Plain ◽

Freshwater Marsh ◽

Freshwater Wetlands ◽

Natural Environments ◽

Carbon Addition ◽

Soil Slurry ◽

The Sanjiang Plain

The progress of dissimilatory iron(III) reduction is widespread in natural environments, particularly in anoxic habitats; in fact, wetland ecosystems are considered as “hotspots” of dissimilatory Fe(III) reduction. In this study, we conducted soil slurry and microbial inoculation anaerobic incubation with glucose, pyruvate, and soluble quinone anthraquinone-2,6-disulphonate (AQDS) additions in freshwater marsh and meadow wetlands in the Sanjiang Plain, to evaluate the role of carbon addition in the rates and dynamics of iron reduction. Dissimilatory Fe(III) reduction in marsh wetlands responded more quickly and showed twice the potential for Fe(III) reduction as that in meadow wetland. Fe(III) reduction rate in marsh and meadow wetlands was 76% and 30%, respectively. Glucose had a higher capacity to enhance Fe(III) reduction than pyruvate, which provides valuable information for the further isolation of Fe reduction bacteria in pure culture. AQDS could dramatically increase potential Fe(III) reduction as an electron shuttle in both wetlands. pH exhibited a negative relationship with Fe(III) reduction. In view of the significance of freshwater wetlands in the global carbon and iron cycle, further profound research is now essential and should explore the enzymatic mechanisms underlying iron reduction in freshwater wetlands.

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Microbially Mediated Biodegradation of Hexahydro-1,3,5-Trinitro-1,3,5- Triazine by Extracellular Electron Shuttling Compounds

Applied and Environmental Microbiology ◽

10.1128/aem.00660-06 ◽

2006 ◽

Vol 72 (9) ◽

pp. 5933-5941 ◽

Cited By ~ 57

Author(s):

Man Jae Kwon ◽

Kevin T. Finneran

Keyword(s):

Humic Substances ◽

Electron Donor ◽

Cell Suspensions ◽

Electron Shuttle ◽

Geobacter Metallireducens ◽

Electron Shuttling ◽

Resting Cell ◽

Molar Basis ◽

Novel Approach

ABSTRACT The potential for humic substances to stimulate the reduction of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) was investigated. This study describes a novel approach for the remediation of RDX-contaminated environments using microbially mediated electron shuttling. Incubations without cells demonstrated that reduced AQDS transfers electrons directly to RDX, which was reduced without significant accumulation of the nitroso intermediates. Three times as much reduced AQDS (molar basis) was needed to completely reduce RDX. The rate and extent of RDX reduction differed greatly among electron shuttle/acceptor amendments for resting cell suspensions of Geobacter metallireducens and G. sulfurreducens with acetate as the sole electron donor. AQDS and purified humic substances stimulated the fastest rate of RDX reduction. The nitroso metabolites did not significantly accumulate in the presence of AQDS or humic substances. RDX reduction in the presence of poorly crystalline Fe(III) was relatively slow and metabolites transiently accumulated. However, adding humic substances or AQDS to Fe(III)-containing incubations increased the reduction rates. Cells of G. metallireducens alone reduced RDX; however, the rate of RDX reduction was slow relative to AQDS-amended incubations. These data suggest that extracellular electron shuttle-mediated RDX transformation is not organism specific but rather is catalyzed by multiple Fe(III)- and humic-reducing species. Electron shuttle-mediated RDX reduction may eventually become a rapid and effective cleanup strategy in both Fe(III)-rich and Fe(III)-poor environments.

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