Facet-dependent reductive dissolution of hematite nanoparticles by Shewanella putrefaciens CN-32

2020 ◽  
Vol 7 (9) ◽  
pp. 2522-2531 ◽  
Author(s):  
Shiwen Hu ◽  
Yundang Wu ◽  
Zecong Ding ◽  
Zhenqing Shi ◽  
Fangbai Li ◽  
...  
Keyword(s):  
Redox Potential ◽  
Iron Reduction ◽  
Reduction Rate ◽  
Shewanella Putrefaciens ◽  
Reductive Dissolution ◽  
Hematite Nanoparticles ◽  
Adsorption Sites ◽  
Dissimilatory Iron Reduction

The hematite {001} possessed more adsorption sites, more positive redox potential, and higher conductivity than {100}, resulting that the dissimilatory iron reduction rate of hematite {001} by S. putrefaciens CN-32 was easier than that of {100}.

Reductive Leaching of Jarosites by Shewanella putrefaciens - Influence of Humic Substances and Chelators in Mineral Dissolution

2015 ◽  
Vol 1130 ◽  
pp. 450-453 ◽  
Author(s):  
Laura Castro ◽  
J.A. Muñoz ◽  
F. González ◽  
M. Luisa Blázquez ◽  
Antonio Ballester
Keyword(s):  
Humic Substances ◽  
Biofilm Formation ◽  
Iron Reduction ◽  
Cell Attachment ◽  
Reduction Rate ◽  
Shewanella Putrefaciens ◽  
Microbial Reduction ◽  
Mineral Dissolution ◽  
Electron Shuttling ◽  
Reductive Leaching

The anaerobic bioreduction of three Fe (III) ores by a type strain of Shewanella putrefaciens has been investigated. The release of ferrous ion indicated the microbial reduction of jarosite and promotes the subsequent secondary mineralization, leading to the formation of various iron-nearing minerals. In addition, the influence of citrate and EDTA in the medium acting as chelating agents, and an electron shuttling molecule were studied. While the citrate and humic substances increased the iron reduction rate, AQDS inhibit the mineral bioreduction and dissolution. S. putrefaciens do not have the necessity of the direct contact between cells and jarosites and, in consequence, cell attachment and biofilm formation on the mineral surface is scant.

Fulvic Acid-Mediated Interfacial Reactions on Exposed Hematite Facets during Dissimilatory Iron Reduction

Langmuir ◽  
2021 ◽  
Author(s):  
Shiwen Hu ◽  
Yundang Wu ◽  
Fangbai Li ◽  
Zhenqing Shi ◽  
Chao Ma ◽  
...  
Keyword(s):  
Fulvic Acid ◽  
Iron Reduction ◽  
Interfacial Reactions ◽  
Dissimilatory Iron Reduction

scholarly journals Enhancement of iron toxicity in L929 cells by d-glucose: accelerated(re-)reduction

2002 ◽  
Vol 368 (2) ◽  
pp. 517-526 ◽  
Author(s):  
Ilka LEHNEN-BEYEL ◽  
Herbert de GROOT ◽  
Ursula RAUEN
Keyword(s):  
Ethyl Ester ◽  
Iron Reduction ◽  
Cell Damage ◽  
Reduction Rate ◽  
Fold Increase ◽  
Iron Complex ◽  
Iron Toxicity ◽  
Intracellular Iron ◽  
Iron Valence ◽  
Iron Pool

It has recently been shown that an increase in the cellular chelatable iron pool is sufficient to cause cell damage. To further characterize this kind of injury, we artificially enhanced the chelatable iron pool in L929 mouse fibroblasts using the highly membrane-permeable complex Fe(III)/8-hydroxyquinoline. This iron complex induced a significant oxygen-dependent loss of viability during an incubation period of 5h. Surprisingly, the addition of d-glucose strongly enhanced this toxicity whereas no such effect was exerted by l-glucose and 2-deoxyglucose. The assumption that this increase in toxicity might be due to an enhanced availability of reducing equivalents formed during the metabolism of d-glucose was supported by NAD(P)H measurements which showed a 1.5—2-fold increase in the cellular NAD(P)H content upon addition of d-glucose. To assess the influence of this enhanced cellular reducing capacity on iron valence we established a new method to measure the reduction rate of iron based on the fluorescent iron(II) indicator PhenGreen SK. We could show that the rate of intracellular iron reduction was more than doubled in the presence of d-glucose. A similar acceleration was achieved by adding the reducing agents ascorbate and glutathione (the latter as membrane-permeable ethyl ester). Glutathione ethyl ester, as well as the thiol reagent N-acetylcysteine, also caused a toxicity increase comparable with d-glucose. These results suggest an enhancement of iron toxicity by d-glucose via an accelerated (re-)reduction of iron with NAD(P)H serving as central electron provider and ascorbate, glutathione or possibly NAD(P)H itself as final reducing agent.

scholarly journals Iron and Carbon Isotope Constraints on the Formation Pathway of Iron-Rich Carbonates within the Dagushan Iron Formation, North China Craton

Minerals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Xiaoxue Tong ◽  
Kaarel Mänd ◽  
Yuhao Li ◽  
Lianchang Zhang ◽  
Zidong Peng ◽  
...  
Keyword(s):  
Iron Reduction ◽  
Isotope Composition ◽  
Iron Formation ◽  
Banded Iron Formations ◽  
Organic C ◽  
Formation Pathway ◽  
Wide Range ◽  
Dissimilatory Iron Reduction ◽  
O Isotope ◽  
Iron Formations

Banded iron formations (BIFs) are enigmatic chemical sedimentary rocks that chronicle the geochemical and microbial cycling of iron and carbon in the Precambrian. However, the formation pathways of Fe carbonate, namely siderite, remain disputed. Here, we provide photomicrographs, Fe, C and O isotope of siderite, and organic C isotope of the whole rock from the ~2.52 Ga Dagushan BIF in the Anshan area, China, to discuss the origin of siderite. There are small magnetite grains that occur as inclusions within siderite, suggesting a diagenetic origin of the siderite. Moreover, the siderites have a wide range of iron isotope compositions (δ56FeSd) from −0.180‰ to +0.463‰, and a relatively negative C isotope composition (δ13CSd = −6.20‰ to −1.57‰). These results are compatible with the reduction of an Fe(III)-oxyhydroxide precursor to dissolved Fe(II) through microbial dissimilatory iron reduction (DIR) during early diagenesis. Partial reduction of the precursor and possible mixing with seawater Fe(II) could explain the presence of siderite with negative δ56Fe, while sustained reaction of residual Fe(III)-oxyhydroxide could have produced siderite with positive δ56Fe values. Bicarbonate derived from both DIR and seawater may have provided a C source for siderite formation. Our results suggest that microbial respiration played an important role in the formation of siderite in the late Archean Dagushan BIF.

DNA relatedness of oil-field isolates of Shewanella putrefaciens

1989 ◽  
Vol 35 (10) ◽  
pp. 925-931 ◽  
Author(s):  
Kathleen M. Semple ◽  
James L. Doran ◽  
D. W. S. Westlake
Keyword(s):  
Iron Reduction ◽  
Shewanella Putrefaciens ◽  
Oil Field ◽  
The Other ◽  
Nucleic Acid Hybridization ◽  
Dna Relatedness ◽  
Field Isolates ◽  
Shewanella Species ◽  
Sulfide Production

Classification of several oil-field isolates of Shewanella putrefaciens was assessed by nucleic acid hybridization techniques. The results of DNA – DNA hybridization analysis generally confirmed the phenetic characterization of these isolates and supported the classification of oil-field isolates of S. putrefaciens groups 1, 3, and 4. However, two group 2 isolates were considered to be mistakenly classified. Strain ESSO 1-1 appeared to belong to group 3, a result which was supported by the pattern of 5S rRNA hybridization to restriction digests of genomic DNA, and strain 213 appeared to be a member of group 1. Several of the oil-field strains of S. putrefaciens were found to possess indigenous plasmids, a feature which was not shared by the other strains of S. putrefaciens examined. This study indicates mat these oil-field isolates were more closely related to strains of S. putrefaciens isolated from various environments than to the other Shewanella species (S. benthica and S. hanedai) that shared the important metabolic characteristics of iron reduction and sulfide production from thiosulfate. There was very little genetic relationship found between Shewanella spp. and the other species studied.Key words: Shewanella putrefaciens, DNA relatedness, oil-field iron reduction, sulfide production, taxonomy.

scholarly journals Effects of Carbon Addition on Dissimilatory Fe(III) Reduction in Freshwater Marsh and Meadow Wetlands

2018 ◽  
Vol 10 (11) ◽  
pp. 4309 ◽  
Author(s):  
Xiaoyan Zhu ◽  
Yuxiang Yuan ◽  
Ming Jiang
Keyword(s):  
Iron Reduction ◽  
Reduction Rate ◽  
Negative Relationship ◽  
Sanjiang Plain ◽  
Freshwater Marsh ◽  
Freshwater Wetlands ◽  
Natural Environments ◽  
Carbon Addition ◽  
Soil Slurry ◽  
The Sanjiang Plain

The progress of dissimilatory iron(III) reduction is widespread in natural environments, particularly in anoxic habitats; in fact, wetland ecosystems are considered as “hotspots” of dissimilatory Fe(III) reduction. In this study, we conducted soil slurry and microbial inoculation anaerobic incubation with glucose, pyruvate, and soluble quinone anthraquinone-2,6-disulphonate (AQDS) additions in freshwater marsh and meadow wetlands in the Sanjiang Plain, to evaluate the role of carbon addition in the rates and dynamics of iron reduction. Dissimilatory Fe(III) reduction in marsh wetlands responded more quickly and showed twice the potential for Fe(III) reduction as that in meadow wetland. Fe(III) reduction rate in marsh and meadow wetlands was 76% and 30%, respectively. Glucose had a higher capacity to enhance Fe(III) reduction than pyruvate, which provides valuable information for the further isolation of Fe reduction bacteria in pure culture. AQDS could dramatically increase potential Fe(III) reduction as an electron shuttle in both wetlands. pH exhibited a negative relationship with Fe(III) reduction. In view of the significance of freshwater wetlands in the global carbon and iron cycle, further profound research is now essential and should explore the enzymatic mechanisms underlying iron reduction in freshwater wetlands.

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