Extraction and Characterization of Galactomannan from Seeds of Leucaena leucocephala

2015 ◽  
Vol 1134 ◽  
pp. 213-219 ◽  
Author(s):  
Shazlinda Shirajuddin ◽  
Dzaraini Kamarun ◽  
Nahlah Elkudssiah Ismail ◽  
Mohd Shahezwan Abdul Wahab ◽  
Abdul Rashid Li ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Leucaena Leucocephala ◽  
High Performance ◽  
Drug Carrier ◽  
Total Carbohydrate ◽  
Total Carbohydrate Content ◽  
G Ratio

Galactomannan is a biopolymer polysaccharides mostly obtained from Leguminosae plant have shown an interesting capability as drug carrier. In this study, galactomannan from the seeds ofLeucaenaleucocephalaknown locally as ‘petai belalang’ was obtained by water extraction. The galactomannan was characterized structurally by nuclear magnetic resonance (NMR) and fourier transform infrared spectroscopy (FTIR). Highly molecular weight of galactomannan, > 2.56 x 106Da, was successfully extracted from the plant. Total carbohydrate content of this polysaccharides is 74.84% and composition of galactose and mannose have been confirm by high performance liquid chromatography (HPLC) which is 36.67% and 38.17% respectively. M/G ratio was determined as 1:1. Hence, galactomannan has been successfully extracted from the seeds ofLeucaenaleucocephala.

scholarly journals Chromatographic characterization of products isolated from chrome shavings

1999 ◽  
Vol 42 (3) ◽  
pp. 281-290
Author(s):  
Edivaldo E Garcia ◽  
Cláudia C.M Kimura ◽  
Ariovaldo C Martins ◽  
Gisele O Rocha ◽  
Jorge Nozaki
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Chemical Characterization ◽  
Gel Permeation Chromatography ◽  
Gel Permeation ◽  
Chromatographic Characterization ◽  
Free Aminoacids

Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

scholarly journals Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2870 ◽  
Author(s):  
Eliza Kurek ◽  
Magdalena Michalska-Kacymirow ◽  
Anna Konopka ◽  
Olga Kościuczuk ◽  
Anna Tomiak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Analytical Techniques ◽  
Low Molecular Weight ◽  
Analytical Protocol ◽  
Icp Ms

A fit for purpose analytical protocol was designed towards searching for low molecular weight seleno-compounds in sprouts. Complementary analytical techniques were used to collect information enabling the characterization of selenium speciation. Conceiving the overall characterization of the behavior of selenium, inductively plasma optical mass spectrometry (ICP-MS) was used to determine the total selenium content in entire sprouts as well as in selected extracts or chromatographic fractions. Then, high-performance liquid chromatography combined with ICP-MS (HPLC-ICP-MS) was used to evaluate the presence of inorganic and organic seleno-compounds, with the advantages of being very sensitive towards selenium, but limited by available selenium standard compounds. Finally, ultra-high performance liquid chromatography electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-QqQ-MS/MS) and UHPLC-ESI-Orbitrap-MS/MS were used for the confirmation of the identity of selected compounds and identification of several unknown compounds of selenium in vegetable sprouts (sunflower, onion, radish), respectively. Cultivation of plants was designed to supplement sprouts with selenium by using solutions of selenium (IV) at the concentration of 10, 20, 40, and 60 mg/L. The applied methodology allowed to justify that vegetable sprouts metabolize inorganic selenium to a number of organic derivatives, such as seleno-methylselenocysteine (SeMetSeCys), selenomethionine (SeMet), 5′-seleno-adenosine, 2,3-DHP-selenolanthionine, Se-S conjugate of cysteine-selenoglutathione, 2,3-DHP-selenocysteine-cysteine, 2,3-DHP-selenocysteine-cysteinealanine, glutathione-2,3-DHP-selenocysteine, gamma-Glu-MetSeCys or glutamyl-glycinyl-N-2,3-DHP-selenocysteine.

scholarly journals Isolation and Characterization of a Chemical from Tomato Apices that Stimulates Plant Growth

HortScience ◽  
1990 ◽  
Vol 25 (7) ◽  
pp. 769-771
Author(s):  
Yichun Wang ◽  
Violet Wert ◽  
Muraleedharan Nair ◽  
Stanley Ries
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Zea Mays ◽  
High Performance Liquid Chromatography ◽  
Oryza Sativa ◽  
Liquid Chromatography ◽  
High Performance ◽  
Water Extract ◽  
Isolation And Characterization ◽  
Layer Chromatography

A methanol: water extract of tomato (Lycopersion esculentum Mill.) apices increased the growth of the alga Chlamydomonas reinhardtii. The active substance from the dried shoot apices was purified by C18 flash column and high performance liquid chromatography. The purified extract enhanced the growth of tomato, corn (Zea mays L.), and rice (Oryza sativa L.) seedlings at concentrations less than 1.0 mg·liter-1. With Chlamydomonas, the purified extract increased cell division 111% at 0.1 mg·liter-1 and chlorophyll content 23% at 10 mg·liter-1 in 18 hours. Nuclear magnetic resonance and mass spectroscopy indicated that the purified fraction was a mixture of compounds having sugar moieties. Analysis by thin layer chromatography showed that the fraction was ninhydrin positive and more polar than the known plant hormones studied.

Acid Proteases from Species of Mucor. II. Partial Characterization of the Acid Protease Produced by a Strain of Mucor miehei Isolated in Cuba

1975 ◽  
Vol 53 (3) ◽  
pp. 269-274 ◽  
Author(s):  
W. S. Rickert ◽  
P. A. McBride-Warren
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Detailed Comparison ◽  
Acid Protease ◽  
Total Carbohydrate ◽  
Mucor Miehei ◽  
Total Carbohydrate Content ◽  
Helical Content

The acid protease produced by a strain of Mucor miehei isolated in Cuba was purified by column electrofocusing and partially characterized as to amino-acid composition, molecular weight, helical content, total carbohydrate content, and approximate isoelectric point. A detailed comparison of these results with those reported previously for Mucor miehei protease (Ottesen, M. &Rickert, W. S. (1970) C.R. Trav. Lab. Carlsberg 37, 301) suggested that the two enzymes are similar but not identical. This conclusion was reinforced by an analysis of circular-dichroism spectra.

Studies on the Stereochemical Characterization of N-Methylated Amino Acids

1991 ◽  
Vol 44 (11) ◽  
pp. 1591 ◽  
Author(s):  
SG Ang ◽  
SH Low
Keyword(s):  
Amino Acids ◽  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Mobile Phase ◽  
High Performance ◽  
1H Nuclear Magnetic Resonance ◽  
Chiral Solvating Agent

Various methods that could be used to determine the absolute configuration of N,N- dimethylated amino acids were investigated. Two methods utilizing 1H nuclear magnetic resonance (N.M.R.) spectroscopy were successful. The first involved the conversion of the N,N- dimethylated amino acids into diastereoisomers by reaction with methyl (S)-(+)- mandelate . In the second method, the N,N- dimethylated amino acids were amidated with 3,5-dinitroaniline, and 1H n.m.r. analysis was carried out with the addition of a chiral solvating agent, (R)-(-)-1-(9-anthryl)-2,2,2-trifluoroethanol [(R)-(-)-atfe ]. Another method involving the use of high-performance liquid chromatography ( h.p.l.c .), with bis (L-aspartic cyclohexylamide )copper(II) complex CuII (L- Aspchex )2, as the chiral mobile phase was also examined but was found to be unsuccessful in resolving the D- and L-isomers of N,N- dimethylated amino acids.

scholarly journals Synthesis and Characterization of Tolvaptan Impurities

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Madhuresh Kumar Sethi ◽  
Vijendra Singh Rawat ◽  
Jayaprakash Thirunavukarasu ◽  
Rajakrishna Yerramalla ◽  
Anish Kumar
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
High Performance ◽  
Mass Spectra ◽  
Synthesis And Characterization ◽  
Nuclear Magnetic

Twenty-six possible as well as observed impurities during the preparation of Tolvaptan have been identified, prepared, and characterized by HPLC (high performance liquid chromatography), NMR (nuclear magnetic resonance), and mass spectra. Control of these impurities, formed during various stages of Tolvaptan preparation, has been mentioned in this paper.

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