Isolation and Characterization of Laccase Activity in a Novel Bacillus amyloliquefaciens LC02

2011 ◽  
Vol 183-185 ◽  
pp. 773-777 ◽  
Author(s):  
Jun Bo Pan ◽  
Min Zhao ◽  
Lei Lu ◽  
Mei Hui Du ◽  
Guo Fu Li ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Laccase Activity ◽  
Copper Ions ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Isolation And Characterization ◽  
Physiological And Biochemical

Bacterial strains exhibiting laccase activity were isolated from the forest soil. A strain LC02 with syringaldazine oxidation ability was obtained using enrichment medium supplemented with copper ions. The isolated strain was identified as Bacillus amyloliquefaciens using physiological and biochemical tests as well as 16S rDNA sequence analysis. The characterization of spore laccase activity was investigated. The result showed that the optimum pH and temperature of the enzyme was 6.6 and 70°C, respectively. A great thermostability was observed for the spore laccase at 70°C. Laccase activity was strongly inhibited by 0.1 mmol/L NaN3, dithiothreitol and cysteine.

Isolation and Characterization of a Novel Bacillus subtilis WD23 Exhibiting Laccase Activity from Forest Soil

2010 ◽  
Vol 113-116 ◽  
pp. 725-729 ◽  
Author(s):  
Chun Lei Wang ◽  
Min Zhao ◽  
De Bin Li ◽  
Dai Zong Cui ◽  
Hong Yi Yang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Forest Soil ◽  
Half Life ◽  
Laccase Activity ◽  
Methyl Violet ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Alizarin Red ◽  
Optimum Growth Temperature ◽  
Isolation And Characterization

The strain Bacillus sp. WD23 exhibiting laccase activity was screened from forest soil. The M9 medium containing Cu2+ was used for enriching and isolating bacterial strains capable of oxidizing syringaldazine (SGZ). One isolated strain was identified as Bacillus subtilis WD23 based on the results of physiological and biochemical tests and 16S rDNA sequence analysis. The strain WD23 could grow at temperatures ranging from 20 to 55°C and showed optimum growth temperature and pH at 25°C and 7.0, respectively. The sporulation rate of the strain clearly correlated well with the laccase activity. The temperature half-life of the spore laccase was 2.5 h at 80°C and the pH half-life was 15 d at pH 9.0. Its spore laccase could decolorized 50~90% of Remazol brilliant blue R (RBBR), alizarin red, congo red, methyl orange and methyl violet, which suggests the potential application of spore laccase in dyestuff treatment.

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

Isolation and Cultivation Optimization of Chlorimuron-Ethyl Degrading Strains

2010 ◽  
Vol 113-116 ◽  
pp. 913-918
Author(s):  
De Bin Li ◽  
Lei Lu ◽  
Min Zhao
Keyword(s):  
Sequence Analysis ◽  
Bacillus Licheniformis ◽  
Contaminated Soil ◽  
Hplc Analysis ◽  
Bacterial Strains ◽  
Cultivation Conditions ◽  
Biochemical Tests ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Chlorimuron Ethyl

Bacterial strains with chlorimuron-ethyl degrading ability were isolated for bioremediation of contaminated soil. Six strains were obtained from chlorimuron-ethyl contaminated soil by enrichment cultivation. HPLC analysis indicated that two strains (A4 and A5) demonstrated high degradation efficiency than other strains. More than 61% of chlorimuron-ethyl was degraded by the two strains after 24 h. Based on the results of biochemical tests and 16S rDNA sequence analysis, the strain A4 and A5 were identified as Bacillus licheniformis and B. cereus, respectively. The cultivation conditions of the two strains were optimized to increase the biomass production.

Characteristics of Spore-Bound Laccase from Bacillus subtilis WD23 and its Use in Dye Decolorization

2010 ◽  
Vol 113-116 ◽  
pp. 226-230 ◽  
Author(s):  
Chun Lei Wang ◽  
Min Zhao ◽  
Xing Dong Wei ◽  
Tai Lun Li ◽  
Lei Lu
Keyword(s):  
Bacillus Subtilis ◽  
Dye Decolorization ◽  
Luria Bertani ◽  
Biochemical Tests ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Optimum Ph ◽  
Ph Range ◽  
Physiological And Biochemical ◽  
Optimum Ph And Temperature

Treatment of xenobiotic compounds such as textile dyes with bacterial laccases is limited to the acid pH range and moderate temperatures. A bacterial strain, designated as WD23, was isolated from forest soil using Luria-Bertani medium supplemented with 0.4 mmol/L Cu2+. The isolated strain was identified as Bacillus subtilis by physiological and biochemical tests and 16S rDNA sequence analysis. Here we charactered the spore-bound laccase of B. subtilis WD23 and used the laccase to decolorize dyes. The spores of the strain showed laccase-like activity, oxidizing syringaldazine, 2,6-dimethoxyphenol and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate acid)(ABTS). The optimum pH and temperature for the spore-bound laccase were 6.8 and 60°C, respectively. It also showed higher stabilities over a broad pH range, the pH half-life was more than 6 months at pH 6.8. The spore laccase could efficiently decolorize 50~90% of anthraquinone and azo dyes in 24 h. The spore laccase can play an important role in bioremediation.

scholarly journals The Utilization of Rum Slops by Marine Bacteria. II. Characterization of Efficient Strains.

1969 ◽  
Vol 63 (2) ◽  
pp. 189-194
Author(s):  
D. R. Hale ◽  
T. R. Tosteson
Keyword(s):  
Molecular Weight ◽  
Sea Water ◽  
Necessary Condition ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Marine Communities ◽  
Water Media ◽  
Physiological And Biochemical

Twenty bacterial isolates from selected marine communities were obtained employing solid, modified sea water media contain ing slops. Thirteen basic morphological, cytological, physiological and biochemical tests were conducted to characterize six of the strains that grew most successfully on the slops media. The ability to hydrolyze high molecular weight sugars and proteins appears to be a necessary condition for the successful growth of some of these isolates on slops media. Tentative identifications of these bacterial strains were made.

scholarly journals Isolation and Characterization of Bacillus Sp. Capable of Degradating Alkali Lignin

2021 ◽  
Vol 9 ◽  
Author(s):  
Jing Yang ◽  
Jian Zhao ◽  
Jianchun Jiang ◽  
Hao Xu ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Ligninolytic Enzyme ◽  
Enzyme Analysis ◽  
16S Rdna Sequence ◽  
Alkali Lignin ◽  
16S Rdna Sequence Analysis ◽  
Isolation And Characterization ◽  
Azure B ◽  
Physical And Chemical

Alkali lignin-degrading Bacillus were isolated from forest soils in China and were identified as Bacillus subtilis TR-03 and Bacillus cereus TR-25 by 16S rDNA sequence analysis. Wherein TR-03 displayed optimal 26.72% alkali lignin (2 g/L) degradation at 7 days and 71.23% of Azure-B (0.01%) decolorization at 36 h of cultivation at 37°C. Ligninolytic enzyme analysis revealed that TR-03 was capable of depolymerizing alkali lignin effectively by the producing of lignin peroxidase and laccase, wherein higher laccase activity was cell-associated. At last, the physical and chemical changes of lignin via SEM and FTIR analysis was further observed to prove the lignin degradation by Bacillus subtilis TR-03.

scholarly journals Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp.

2017 ◽  
Vol 15 (1) ◽  
pp. 15-20 ◽  
Author(s):  
YOPI YOPI ◽  
APRIDAH CAMELIAWATI DJOHAN ◽  
NANIK RAHMANI ◽  
ALIFAH MAFATIKHUL JANNAH
Keyword(s):  
Marine Bacteria ◽  
Bacillus Sp ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Isolation And Characterization ◽  
Bacillus Tequilensis ◽  
Optimum Ph ◽  
Tlc Analysis ◽  
Identification And Characterization

Yopi, Djohan AC, Rahmani N, Jannah AM. 2017. Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp. Biofarmasi (Rumphius J Nat Prod Biochem) 15: 15-20. Isolation, identification, and characterization of mannanase, xylanase and cellulase producing indigenous marine bacteria have been conducted from total 20 isolates. Based on 16S rDNA sequence analysis, three potential isolates are obtained and identified as Bacillus subtilis (M8), Bacillus tequilensis (X4) and Bacillus cereus (C9). The potential strains M8, X4 and C9 can produce mannanase, xylanase and cellulase activities such as 9.5 U/mL; 0.36U/mL;0.56U/mL with optimum pH and temperature 6.0;50oC, 5.5;70oC and 8;50oC, respectively. Based on the TLC analysis, mannanase from M8 and xylanase from X4 has potential to hydrolyzed mannan and xylan for producing oligosaccharides with size around tri-hexasaccharides as a main product.

scholarly journals Isolation and characterization of algicidal bacteria and its effect on a musty odor-producing cyanobacterium Dolichospermum crassum in a reservoir

2016 ◽  
Vol 17 (3) ◽  
pp. 792-798 ◽  
Author(s):  
Taketoshi Shimizu ◽  
Takuya Oda ◽  
Hiroyuki Ito ◽  
Ichiro Imai
Keyword(s):  
Heterotrophic Bacteria ◽  
Peak Frequency ◽  
Algicidal Bacteria ◽  
Bacterial Strains ◽  
Sequence Analyses ◽  
16S Rdna Sequence ◽  
Isolation And Characterization ◽  
Musty Odor ◽  
Phytoplankton Monitoring

Algicidal bacteria that attack Dolichospermum crassum were isolated from the Karasuhara Reservoir in October 2010. Phytoplankton monitoring was performed from April 2010 to March 2011, and D. crassum was detected from August to November. At its peak frequency (in early October), it accounted for 23% of all phytoplankton cells. Heterotrophic bacteria were isolated from the surface water, and an algicidal assay was conducted. As a result, 3 out of 47 bacterial strains showed strong algicidal activity, and they completely destroyed the trichomes of D. crassum. An initial inoculation dose of only 1.0 × 102 cells ml−1 of these strains was enough to digest D. crassum. These strains were identified as Rheinheimera spp. according to 16S rDNA sequence analyses. This is the first report about algicidal bacteria that attack D. crassum. Algicidal bacteria could be key agents for controlling D. crassum in reservoirs.

scholarly journals Isolation and characterization of streptococcus bovis from rumencontent of awassi sheep in iraq

2013 ◽  
Vol 12 (1) ◽  
pp. 44
Author(s):  
A. J. A. Al emery
Keyword(s):  
Basal Medium ◽  
Brain Heart Infusion ◽  
Selective Medium ◽  
Streptococcus Bovis ◽  
Biochemical Tests ◽  
Potassium Tellurite ◽  
Awassi Sheep ◽  
Isolation And Characterization ◽  
Physiological And Biochemical

This is the first study in Iraq aimed to isolate and characterize Streptococcus bovis from rumen of Awassi sheep .Ten sheep with different ages fed on grain base diet for three days were used to collect 20 ruminal fluid samples twice at fourth and fifth days by rumenocentesis method , samples cultured on selective media (Modified membrane-bovis agar(M-BA) ,broth of basal medium and modified blood brain heart infusion) ,the isolates were identified according to their morphological, physiological ,biochemical tests and serological by Lancefield group.Cultural characteristic on the selective medium M-BA showed two types of streptococci :first type (23) comprised the majority of isolates ,this type characterized by high acid producing streptococci formed mucoid ,creamy ,orange –centered colonies and second type (4) characterize by low acid producing formed small white colonies .Morphologically the isolates were identified as gram positive ,the cells were oval or spherical , singles, pairs & short chains of 4 to 8 cells. The organisms were found to full under the Lancefield group D.All isolated bacteria grew on broth of basal medium post incubation at 45C˚ ,but not grew at 10C˚ and 50C˚. Adding of 2% NaCl permit the growth, while in 6.5% NaCl didn’t grow. And did not grew on adding 0.04 % Potassium tellurite .All isolates produced lactic acid but ammonia production from arginine was negative, no hemolysis on blood agar . All isolates ferment starch, lactose, fructose, glucose, raffinose and cellobiose.The results of culturing and Physiological and biochemical tests showed that 27 isolates has the characterictices of Streptococcus bovis in 3 types (S1,S2 &S3) ,first type of colony divided into two strain (S115)(S28)according to difference In arabinose. And (S3) called on the second type white pigmented isolates which did not ferment inulin

scholarly journals Isolation and Characterization of Bacteria Resistant to Metallic Copper Surfaces

2010 ◽  
Vol 76 (5) ◽  
pp. 1341-1348 ◽  
Author(s):  
Christophe Esp�rito Santo ◽  
Paula Vasconcelos Morais ◽  
Gregor Grass
Keyword(s):  
Copper Ions ◽  
Copper Alloys ◽  
Metallic Copper ◽  
Copper Ion ◽  
Copper Surface ◽  
Bacterial Strains ◽  
Copper Surfaces ◽  
Isolation And Characterization ◽  
Detoxification Systems

ABSTRACT Metallic copper alloys have recently attracted attention as a new antimicrobial weapon for areas where surface hygiene is paramount. Currently it is not understood on a molecular level how metallic copper kills microbes, but previous studies have demonstrated that a wide variety of bacteria, including Escherichia coli, Staphylococcus aureus, and Clostridium difficile, are inactivated within minutes or a few hours of exposure. In this study, we show that bacteria isolated from copper alloy coins comprise strains that are especially resistant against the toxic properties exerted by dry metallic copper surfaces. The most resistant of 294 isolates were Gram-positive staphylococci and micrococci, Kocuria palustris, and Brachybacterium conglomeratum but also included the proteobacterial species Sphingomonas panni and Pseudomonas oleovorans. Cells of some of these bacterial strains survived on copper surfaces for 48 h or more. Remarkably, when these dry-surface-resistant strains were exposed to moist copper surfaces, resistance levels were close to those of control strains and MICs for copper ions were at or below control strain levels. This suggests that mechanisms conferring resistance against dry metallic copper surfaces in these newly isolated bacterial strains are different from well-characterized copper ion detoxification systems. Furthermore, staphylococci on coins did not exhibit increased levels of resistance to antibiotics, arguing against coselection with copper surface resistance traits.

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