scholarly journals The Utilization of Rum Slops by Marine Bacteria. II. Characterization of Efficient Strains.

1969 ◽  
Vol 63 (2) ◽  
pp. 189-194
Author(s):  
D. R. Hale ◽  
T. R. Tosteson
Keyword(s):  
Molecular Weight ◽  
Sea Water ◽  
Necessary Condition ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Marine Communities ◽  
Water Media ◽  
Physiological And Biochemical

Twenty bacterial isolates from selected marine communities were obtained employing solid, modified sea water media contain ing slops. Thirteen basic morphological, cytological, physiological and biochemical tests were conducted to characterize six of the strains that grew most successfully on the slops media. The ability to hydrolyze high molecular weight sugars and proteins appears to be a necessary condition for the successful growth of some of these isolates on slops media. Tentative identifications of these bacterial strains were made.

Isolation and Characterization of Laccase Activity in a Novel Bacillus amyloliquefaciens LC02

2011 ◽  
Vol 183-185 ◽  
pp. 773-777 ◽  
Author(s):  
Jun Bo Pan ◽  
Min Zhao ◽  
Lei Lu ◽  
Mei Hui Du ◽  
Guo Fu Li ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Laccase Activity ◽  
Copper Ions ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Isolation And Characterization ◽  
Physiological And Biochemical

Bacterial strains exhibiting laccase activity were isolated from the forest soil. A strain LC02 with syringaldazine oxidation ability was obtained using enrichment medium supplemented with copper ions. The isolated strain was identified as Bacillus amyloliquefaciens using physiological and biochemical tests as well as 16S rDNA sequence analysis. The characterization of spore laccase activity was investigated. The result showed that the optimum pH and temperature of the enzyme was 6.6 and 70°C, respectively. A great thermostability was observed for the spore laccase at 70°C. Laccase activity was strongly inhibited by 0.1 mmol/L NaN3, dithiothreitol and cysteine.

scholarly journals Characterization of onion soft rot bacteria in Bangladesh

2017 ◽  
Vol 52 (3) ◽  
pp. 209-220
Author(s):  
MM Rahman ◽  
MAA Khan ◽  
IH Mian ◽  
AM Akanda ◽  
MZ Alam
Keyword(s):  
Bacterial Pathogens ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Carotovorum Subsp ◽  
O 15 ◽  
O 69 ◽  
Physiological And Biochemical

A study was undertaken for characterization and identification of the soft rot causing bacterial pathogens of onion. Bacterial pathogens were isolated from soft rotted stored onions of different varieties and locations of Bangladesh. Altogether 73 bacterial isolates were isolated from soft rotted onions. Among them, twelve soft rot-positive isolates were selected for characterization and identification on the basis of more virulence. Physiological and biochemical tests were performed following standard methods for characterization and identification of selected soft rot bacterial isolates. Seven isolates namely O-03, O-18, O-69, O-72, O-130, O-156 and O-180 were identified as Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora), two isolates O-101 and O-118 were identified as E. chrysanthemi and three isolates O-05, O-14 and O-15 were the members of Burkholderia cepacia.Bangladesh J. Sci. Ind. Res. 52(3), 209-220, 2017

scholarly journals The Utilization of Rum Slops by Marine Bacteria. I. Isolation of Efficient Strains.

1969 ◽  
Vol 63 (2) ◽  
pp. 180-188
Author(s):  
T. R. Tosteson ◽  
D. R. Hale
Keyword(s):  
Liquid Culture ◽  
Sea Water ◽  
Culture Media ◽  
Growth Inhibitors ◽  
Bacterial Strains ◽  
Marine Communities ◽  
Sea Grass ◽  
Water Media ◽  
Marine Mud ◽  
Colony Shape

Bacterial strains have been isolated from selected marine communities, and their ability to utilize and/or detoxify the crude slops has been assessed. These bacterial strains were isolated from 1) marine mud, 2) decaying sea grass, Thalassia testudinum, 3) the surfaces of the roots of the mangrove Rhizophora mangle and 4) gravel sediment. The isolates were grown on solid modified sea water media containing slops. A total of twenty isolates were obtained from the slops media. Colony shape and color of these strains were determined. The slops isolates were grown in liquid culture media in the presence of slops. Eight of the original isolates grew in this media, showing little tendency to form aggregates. The growth of these strains was examined in the presence of variable concentrations of the slops. The results of this study indicate that the growth inhibitors in the slops can be partially detoxified by these bacterial strains, and the remaining material utilized for population growth.

scholarly journals Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides

2002 ◽  
Vol 68 (9) ◽  
pp. 4390-4398 ◽  
Author(s):  
S. A. F. T. van Hijum ◽  
G. H. van Geel-Schutten ◽  
H. Rahaoui ◽  
M. J. E. C. van der Maarel ◽  
L. Dijkhuizen
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Lactobacillus Reuteri ◽  
Bacterial Strains ◽  
Potential Health ◽  
Isolation And Characterization ◽  
A Cell ◽  
Encoding Gene ◽  
First Time

ABSTRACT Fructosyltransferase (FTF) enzymes produce fructose polymers (fructans) from sucrose. Here, we report the isolation and characterization of an FTF-encoding gene from Lactobacillus reuteri strain 121. A C-terminally truncated version of the ftf gene was successfully expressed in Escherichia coli. When incubated with sucrose, the purified recombinant FTF enzyme produced large amounts of fructo-oligosaccharides (FOS) with β-(2→1)-linked fructosyl units, plus a high-molecular-weight fructan polymer (>107) with β-(2→1) linkages (an inulin). FOS, but not inulin, was found in supernatants of L. reuteri strain 121 cultures grown on medium containing sucrose. Bacterial inulin production has been reported for only Streptococcus mutans strains. FOS production has been reported for a few bacterial strains. This paper reports the first-time isolation and molecular characterization of (i) a Lactobacillus ftf gene, (ii) an inulosucrase associated with a generally regarded as safe bacterium, (iii) an FTF enzyme synthesizing both a high molecular weight inulin and FOS, and (iv) an FTF protein containing a cell wall-anchoring LPXTG motif. The biological relevance and potential health benefits of an inulosucrase associated with an L. reuteri strain remain to be established.

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

Characterization of Agrobacterium isolates from stone fruits in Ontario

1978 ◽  
Vol 56 (18) ◽  
pp. 2309-2311 ◽  
Author(s):  
B. N. Dhanvantari
Keyword(s):  
Sweet Cherry ◽  
The Other ◽  
Stone Fruits ◽  
Agrobacterium Radiobacter ◽  
Biochemical Tests ◽  
Nursery Stock ◽  
Physiological And Biochemical

Isolations made from 68 naturally infected peach, plum, and sweet cherry nursery stock from the Niagara Peninsula and southwestern Ontario yielded 30 pathogenic isolates of Agrobacterium. These isolates were separated into two groups by 17 physiological and biochemical tests. The predominant group, consisting of 27 isolates, was 3-ketolactose negative and corresponded to biotype 2, and the other, consisting of only three isolates, corresponded to biotype 1 of Agrobacterium radiobacter var. tumefaciens sensu Keane, Kerr, and New (1970). Among the pathogenic isolates, 25 were sensitive and 5 were resistant to the bacteriocin produced by strain 84 of A. radiobacter var. radiobacter sensu Keane. Kerr, and New (1970).

scholarly journals Nocardia takedensis sp. nov., isolated from moat sediment and scumming activated sludge

2005 ◽  
Vol 55 (1) ◽  
pp. 433-436 ◽  
Author(s):  
Hideki Yamamura ◽  
Masayuki Hayakawa ◽  
Youji Nakagawa ◽  
Tomohiko Tamura ◽  
Tetsuro Kohno ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Reference Strain ◽  
16S Rrna Gene Sequencing ◽  
Morphological Characterization ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Sequencing Studies ◽  
Rrna Gene Sequencing ◽  
Physiological And Biochemical

Chemotaxonomic and morphological characterization of two actinomycete strains, MS1-3T and AS4-2, respectively isolated from moat sediment and scumming activated sludge, was carried out. This characterization clearly demonstrated that strains MS1-3T and AS4-2 belong to the genus Nocardia. 16S rRNA gene sequencing studies showed that these isolates are most closely related to Nocardia beijingensis (98·1–98·3 % similarity), Nocardia brasiliensis (97·9–98·0 %) and Nocardia tenerifensis (97·8–97·9 %). However, the results of DNA–DNA hybridizations and physiological and biochemical tests showed that strains MS1-3T and AS4-2 could be differentiated from their closest phylogenetic relatives both genotypically and phenotypically. It is proposed that the two isolates be classified as representatives of a novel species of Nocardia, Nocardia takedensis sp. nov. The type strain is MS1-3T (=NBRC 100417T=DSM 44801T); AS4-2 (=NBRC 100418=DSM 44802) is a reference strain.

scholarly journals Pedobacter glucosidilyticus sp. nov., isolated from dry riverbed soil

2010 ◽  
Vol 60 (1) ◽  
pp. 229-233 ◽  
Author(s):  
Xuesong Luo ◽  
Zhang Wang ◽  
Jun Dai ◽  
Lei Zhang ◽  
Jun Li ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
The Novel ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
Major Respiratory Quinone ◽  
Physiological And Biochemical

Two Gram-staining-negative, rod-shaped, non-spore-forming bacterial strains, 1-2T and 1-4 were isolated from dry riverbed soil collected from the Xietongmen area of Tibet, China. On the basis of 16S rRNA gene sequence similarity, the novel strains were shown to belong to the genus Pedobacter, sharing <95 % sequence similarity with all recognized species of the genus Pedobacter. The major respiratory quinone was MK-7 and the predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising iso-C16 : 1 ω7c and/or C16 : 1 ω6c). The DNA G+C contents were 37.2–37.6 mol%. Chemotaxonomic data supported the affiliation of the two new isolates to the genus Pedobacter and the results of physiological and biochemical tests confirmed that the new strains differed significantly from the recognized species of the genus Pedobacter. Therefore, the new isolates represent a novel species within the genus Pedobacter, for which the name Pedobacter glucosidilyticus sp. nov. is proposed. The type strain is 1-2T (=CCTCC AB 206110T=KCTC 22438T).

Isolation and characterization of n-butane-utilizing microorganisms

1972 ◽  
Vol 18 (8) ◽  
pp. 1191-1195 ◽  
Author(s):  
A. G. McLee ◽  
Agnes C. Kormendy ◽  
M. Wayman
Keyword(s):  
Partial Pressure ◽  
Growth Rates ◽  
Bacterial Isolates ◽  
Partial Pressure Of Oxygen ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Partial Pressures ◽  
High Partial Pressure

Fifteen bacterial strains and four molds capable of growth on n-butane were isolated and partially classified. The bacteria were mostly Arthrobacter sp. and Brevibacterium sp.; among the molds, Penicillium nigricans, Allescheria boydii, and Graphium cumeiferum were identified, while the remaining mold had the appearance of Gliocladium, but was not firmly identified. Although able to grow on other alkanes and orthodox media, the bacterial isolates could not use methane. Growth rates on n-butane were unaffected by varying air or substrate partial pressures in the range of 10–90% atmosphere. High partial pressure of oxygen was inhibitory to most bacterial isolates, the degree of inhibition varying widely, however. Growth rates on n-butanol and on glucose were significantly higher than those on n-butane. Among the molds, only the Graphium would grow well in submerged, shaking culture.

Pseudovibrio ascidiaceicola sp. nov., isolated from ascidians (sea squirts)

2006 ◽  
Vol 56 (2) ◽  
pp. 343-347 ◽  
Author(s):  
Yukiyo Fukunaga ◽  
Midori Kurahashi ◽  
Kenji Tanaka ◽  
Kensuke Yanagi ◽  
Akira Yokota ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Early Stage ◽  
Stationary Growth Phase ◽  
Fatty Acid Analysis ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Gene Sequence Analysis ◽  
Sea Squirts ◽  
Physiological And Biochemical

Two bacterial strains, F423T and F10102, were isolated from two ascidians, Polycitor proliferus and Botryllidae sp., respectively, which were collected from a beach on the Boso peninsula in Japan. Cells of both isolates were motile, rod-shaped and formed star-shaped aggregates in the early stage of exponential growth, but were coccoid in stationary growth phase. The results of 16S rRNA gene sequence analysis, fatty acid analysis, DNA–DNA hybridization experiments and physiological and biochemical tests indicated that the two strains were members of a novel species of the genus Pseudovibrio for which the name Pseudovibrio ascidiaceicola sp. nov. is proposed. The type strain is F423T (=NBRC 100514T=IAM 15084T=DSM 16392T=KCTC 12308T).

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