Isolation and Identification of Strains with High Chitin Deacetylase Activity

2012 ◽  
Vol 457-458 ◽  
pp. 476-479
Author(s):  
Jing Xuan Gou ◽  
Wen Bin Dong ◽  
Qiao Zeng ◽  
Jing Jing Zhang
Keyword(s):  
Food Industry ◽  
Analysis Data ◽  
Soil Samples ◽  
Optimum Temperature ◽  
Isolation And Identification ◽  
Chitin Deacetylase ◽  
Physiological And Biochemical Characteristics ◽  
Optimum Ph ◽  
Physiological And Biochemical ◽  
Rdna Analysis

Chitosan is an aboundant biopolymer like cellose. To bioprocess them with chitin deacetylase (CDA), the products will be used in medicine and food industry. [In order to get new strains with significant ability of (CDA) Twenty eight strains producing CDA were isolated and screened out from the soil samples by color reaction in plate medium. The strain F2-7-3 was screened out from these strains for the highest CDA activity, which can reach more than 250U/mL. The paranitroacetanilide was used as the substrate, the optimum temperature for enzyme activity was determined to be 50°C and the optimum pH was 7.0. It was studied by method of morphological, physiological and biochemical characteristics and 16S rDNA analysis. Data shows that the strain was Rhodococcus sp.

scholarly journals Isomalto-Oligosaccharides Produced by Endodextranase Shewanella sp. GZ-7 From Sugarcane Plants

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095328
Author(s):  
Xin Liu ◽  
Tian Deng ◽  
Xueqin Liu ◽  
Xiaohua Lai ◽  
Yanli Feng ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
High Performance ◽  
Deoxyribonucleic Acid ◽  
Gene Sequencing ◽  
Optimal Conditions ◽  
Optimum Temperature ◽  
Physiological And Biochemical Characteristics ◽  
Ph Range ◽  
Layer Chromatography ◽  
Physiological And Biochemical

Oligosaccharides have important alimental and medical applications. Dextranase has been used to produce isomalto-oligosaccharides (IMOs). In this study, we isolated dextranase-producing bacteria from sugarcane-cultivated soil. Identification of the isolate based on its phenotypical, physiological, and biochemical characteristics, as well as 16S ribosomal deoxyribonucleic acid gene sequencing yielded Shewanella sp. strain GZ-7. The molecular weight of the dextranase produced by this strain was 100-135 kDa. The optimum temperature and pH for dextranase production were 40 °C and 7.5, respectively. The enzyme was found to be stable at the pH range of 6.0-8.0 and the temperature range of 20 °C-40 °C. Thin-layer chromatography and high-performance liquid chromatography of the enzymolysis products of the substrate confirmed the enzyme to be endodextranase. Under the optimal conditions, the ratio of IMOs could reach 91.8% of the hydrolyzate. The final products were found to efficiently scavenge the 2,2-diphenyl-1-picrylhydrazyl, hydroxyl, and superoxide anion radicals. In general, dextranase and hydrolyzates have high potential prospects for application in the future.

Isolation and Identification of a PCBs-Degrading Strain

2012 ◽  
Vol 518-523 ◽  
pp. 229-232
Author(s):  
Zhe Zhao ◽  
Sheng Guang Zhuo ◽  
Xiu Yan Zhou ◽  
Lan Ying Zhang
Keyword(s):  
Water Environment ◽  
Underground Water ◽  
Dna Fingerprint ◽  
Vibrio Splendidus ◽  
Optimum Conditions ◽  
Salt Tolerant ◽  
Isolation And Identification ◽  
Physiological And Biochemical Characteristics ◽  
Dynamic Resolution ◽  
Physiological And Biochemical

A salt-tolerant PCBs degrading strain B2.6 which can use PCBs as the sole carbon source and energy was isolated from the deep sea sediments of southern Yellow Sea. It was identified as Vibrio splendidus, according to morphological, physiological and biochemical characteristics and 16SrDNA sequence analysis. The experimental results suggested that the optimum conditions for the degradation of PCBs are temperature 30°C, pH 7 to 8, the concentration of NaCl was 150g•L-1. Under the optimum conditions, the PCBs degradation could reach 98.27% in 72h which concentration was below 0.6mg•L-1. The genome was amplified with bacterium 16SrDNA primer. The amplified product was purified and sequenced forphylogenetic analyses with Genbank. The bacterium B2.6 was identified as Vibrio splendidus. RAPD analyses of B2.6 produced stable DNA fingerprint. These studies have provided parameter for the further dynamic resolution of bacterium clusters for the rehabilitation of PCBs contaminated underground water environment with B2.6 PRB method.

Isolation and Identification of a Low Density Polyethylene Degradable Bacterium

2011 ◽  
Vol 356-360 ◽  
pp. 172-177
Author(s):  
Yi Ling Song ◽  
Jun Yang ◽  
Hong Pan ◽  
Jing Wen Li ◽  
Jian Bo Zhu ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Gene Sequence ◽  
Synthetic Polymers ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Isolation And Identification ◽  
Physiological And Biochemical Characteristics ◽  
Indian Meal ◽  
The Difference ◽  
Physiological And Biochemical

Polyethylene (PE) is one of the most abundant commercially produced synthetic polymers which have leaded the seriously solid pollution. A Gram positive rod form bacterium T6 has been isolated from the guts of Indian meal moth Plodia interpunctella (Hübner). According to the phenotypic, Physiological and biochemical characteristics, and 16S rRNA gene sequence, T6 is identified as Baccillus megaterium. Scanning electron microscope (SEM) reveals the polyethylene can be degraded significantly by T6 from the LDPE surface. The tensile strength of the LDPE films which is degraded by T6 strain, displays decreasing tendency as the incubation time increase. After 28 days’ incubation, the tensile strength decreases 5.8% and the maximum decrement reaches 18.4%. The difference between the tensile strength value of day 21 and day 28 are not significant (P <0.05). The results indicate T6 strain can degraded the LDPE film.

scholarly journals Nocardia artemisiae sp. nov., an endophytic actinobacterium isolated from a surface-sterilized stem of Artemisia annua L.

2011 ◽  
Vol 61 (12) ◽  
pp. 2933-2937 ◽  
Author(s):  
Guo-Zhen Zhao ◽  
Jie Li ◽  
Wen-Yong Zhu ◽  
Hans-Peter Klenk ◽  
Li-Hua Xu ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Artemisia Annua ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Physiological And Biochemical Characteristics ◽  
Gene Sequence Analysis ◽  
Endophytic Actinobacterium ◽  
Optimum Ph ◽  
Established Species ◽  
Physiological And Biochemical

A novel actinobacterium, designated YIM 65623T, was isolated from a surface-sterilized stem of Artemisia annua L. Strain YIM 65623T had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus Nocardia. Growth occurred with 0–7 % (w/v) NaCl (optimum 0–3 %), at pH 5.0–9.0 (optimum pH 6.0) and at 10–37 °C (optimum 20–28 °C). Comparative 16S rRNA gene sequence analysis showed that strain YIM 65623T constituted a distinct sublineage within the genus Nocardia and displayed 94.1–98.2 % sequence similarity to members of established species in the genus Nocardia. However, DNA–DNA relatedness and physiological and biochemical characteristics showed that strain YIM 65623T could be differentiated from its closest phylogenetic relatives. The G+C content of the genomic DNA was 69.6 mol%. It is proposed that strain YIM 65623T be classified as a representative of a novel species, Nocardia artemisiae sp. nov. The type strain is YIM 65623T ( = DSM 45379T  = CCTCC AA 209038T).

scholarly journals ISOLASI DAN KARAKTERISASI EKSTRAK KASAR ENZIM BROMELIN DARI BATANG NANAS (Ananas comusus L.merr)

2006 ◽  
Vol 12 (1) ◽  
pp. 75-77
Author(s):  
Nuniek Herdyastuti
Keyword(s):  
Enzyme Activity ◽  
Crude Extract ◽  
Soluble Protein ◽  
Food Industry ◽  
Optimum Temperature ◽  
Ph Variation ◽  
Isolation And Characterization ◽  
Optimum Ph

Brommelain is an enzyme hydrolyze most soluble protein easily and efficiently. This enzyme is used in many industry like food industry. This research aimed to isolation and characterization crude extract brommelain. This enzyme has been extracted from the stems of pineapples to produce crude extract, precipitated with amonium sulfat, and enzyme activity to decided with Bergmeyer methode. The higher activity was 1,996 U/ml in precipitate 40-60 percent amonium sulfat. Optimum temperature and pH to decided temperature and pH variation was detected based on enzyme activity. Characterization to indicate that bromelain has an optimum temperature at 55°C, optimum pH of 7, KM = 5.074 mg/ml and Vmax = 0.666 mg/ml.second.

Purification and Properties of Ribonuclease From Buffalo Milk Whey

1977 ◽  
Vol 40 (6) ◽  
pp. 375-377 ◽  
Author(s):  
AZZA A. ISMAIL ◽  
N. S. AHMED ◽  
M. A. KHORSHID
Keyword(s):  
Deae Cellulose ◽  
Ribonuclease A ◽  
Buffalo Milk ◽  
Optimum Temperature ◽  
Isolation And Identification ◽  
Milk Whey ◽  
Purification And Properties ◽  
Optimum Ph ◽  
Ribonuclease B

A procedure was developed for isolation and identification of ribonuclease from buffalo milk whey. Ribonuclease was precipitated with (NH4)2SO4 between 65 and 90% saturation. The precipitate was dissolved, dialyzed, and fractionated on DEAE-cellulose. Two ribonuclease-rich fractions were collected, i.e. ribonuclease A and B. Ribonuclease A had an optimum pH of 7 .0, and ribonuclease B had an optimum pH of 8.6. Both had an optimum temperature at 38 C. The ribonucleases in the purified state were unstable to heat and their activity decreased as the time of exposure increased. Both enzyme fractions were sensitive to inhibitors. NaCl and NaN3 were stimulatory for ribonuclease A, while ribonuclease B was stimulated only by NaCl.

Isolation and Identification of a Heterotrophic Nitrifying Bacteria and Study on Removal of Nitrite Nitrogen

2015 ◽  
Vol 737 ◽  
pp. 473-476 ◽  
Author(s):  
Qing Man Cui ◽  
Hai Wen Wang ◽  
Wan Ying Wang ◽  
Chun Ying Yuan
Keyword(s):  
Removal Rate ◽  
Morphological Characteristics ◽  
Nitrifying Bacteria ◽  
Isolation And Identification ◽  
Acinetobacter Baumanii ◽  
Shrimp Pond ◽  
Physiological And Biochemical Characteristics ◽  
Nitrite Nitrogen ◽  
Heterotrophic Nitrifying Bacteria ◽  
Physiological And Biochemical

A bacteria strain for efficiently removing nitrite nitrogen was isolated and purified from sediment in shrimp pond, and the morphology of bacteria was observed using scanning electron microscopy, and the homology analysisof 16SrDNA sequence of the strain was conducted. The results showed that the strain was identified as Acinetobacter baumanii according to the morphological characteristics, physiological and biochemical characteristics and 16SrDNA sequence analysis. The isolated strain had a high heterotrophic nitrification function, the removal rate of nitrite nitrogen reached 97.17% after 12 h.

scholarly journals Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra)

2010 ◽  
Vol 60 (4) ◽  
pp. 944-948 ◽  
Author(s):  
Nitcha Chamroensaksri ◽  
Somboon Tanasupawat ◽  
Ancharida Akaracharanya ◽  
Wonnop Visessanguan ◽  
Takuji Kudo ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Molecular Data ◽  
Rrna Gene ◽  
Fermented Fish ◽  
Physiological And Biochemical Characteristics ◽  
The Family ◽  
Optimum Ph ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

A novel strain, designated TP2-8T, was isolated from fermented fish (pla-ra) in Thailand. It stained Gram-positive and the cells were aerobic, endospore-forming rods. The strain grew at pH 6–8 (optimum pH 7), 15–55 °C (optimum 37 °C) and 1–22 % (w/v) NaCl (optimum 5–10 %). It contained meso-diaminopimelic in the cell-wall peptidoglycan. MK-7 and cellular fatty acids anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 were major components. Polar lipids diphosphatidylglycerol and phosphatidylglycerol and unidentified lipids were detected. The DNA G+C content was 37.6 mol%. Comparison of the 16S rRNA gene sequence of strain TP2-8T with those of other members of the family Bacillaceae indicated that it was a member of the genus Gracilibacillus (94.9–99.2 % sequence similarity) and was closely related to Gracilibacillus saliphilus YIM 91119T (99.2 % similarity), G. lacisalsi BH312T (98.6 %), G. orientalis XH-63T (97.7 %), ‘G. quinghaiensis’ YIM C229 (97.7 %) and G. boraciitolerans T-16XT (97.2 %). Strain TP2-8T showed low DNA–DNA relatedness (≤49 %) to G. saliphilus YIM 91119T, G. lacisalsi DSM 19029T, G. orientalis CCM 7326T, ‘G. quinghaiensis’ DSM 17858 and G. boraciitolerans JCM 21714T. On the basis of the physiological and biochemical characteristics and molecular data presented, strain TP2-8T is proposed to represent a novel species, Gracilibacillus thailandensis sp. nov. (type strain TP2-8T =JCM 15569T =PCU 304T =TISTR 1881T).

Isolation and Identification of a Chitin Degrading Bacterium Aeromonas SP D5-23 and Properties of Chitinase

2012 ◽  
Vol 457-458 ◽  
pp. 472-475
Author(s):  
Jing Xuan Gou ◽  
Wen Bin Dong ◽  
Qiao Zeng ◽  
Lei Jin
Keyword(s):  
Enzyme Activity ◽  
Soil Sample ◽  
16S Rdna ◽  
Morphological Analysis ◽  
Chitinase Activity ◽  
Vibrio Alginolyticus ◽  
Optimum Temperature ◽  
Isolation And Identification ◽  
Degrading Bacterium ◽  
Optimum Ph

Chitin is an abundant biopolymer like cellulose that is rather resistant to degradation. In order to develop a bio-digesting method, soil sample in Qinling Mountain were collected for screening the bacteria with high chitinase activity by method of the transparent circle. The strain D5-23 was isolated and screened out from soil, which was found with amazing chitinase acitivity. The ratio of transient circle and colony circle is no less than 10. The strain was then identified as Aeromonas sp according to the sequences of 16S rDNA and morphological analysis. The enzyme activity was studied further, ,data shows that the optimum temperature was 45°C, which is similar to other Aeromonas sp, wheras the optimum pH is 5 and 9, which is more similar to Vibrio alginolyticus TK-22.

Study on Enzymatic Characteristics of Chitin Deacetylase

2011 ◽  
Vol 236-238 ◽  
pp. 996-999 ◽  
Author(s):  
Jun Ang Liu ◽  
Yuan Hao He
Keyword(s):  
Inhibitory Effect ◽  
Enzymatic Properties ◽  
Optimum Temperature ◽  
Chitin Deacetylase ◽  
Activation Effect ◽  
High Productivity ◽  
Enzymatic Characteristics ◽  
Optimum Ph

Chitin deacetylase (CDA) catalyzes the conversion of chitin to chitosan by the deacetylation of N-acetyl-D-glucosamine residues. The results of the fundamental enzymatic properties of chitin deacetylase producing from the high productivity chitin deacetylase strain Z7 show that, the optimum temperature of strain Z7 was 40°C, the optimum pH was 6.5, under the concentration of 1mmol/L, Mg2+, K+, Ca2+and Fe2+had activation effect, of which the strongest activation effect was Mg2+. Pb2+, Cu2+and Mn2+had inhibitory effect, of which the strongest inhibitory effect was Mn2+.

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