Discrimination and Simultaneous Determination of 6-BA and KT by Polarized Synchronous Light Scattering and Synchronous Light Scattering Spectrum

2013 ◽  
Vol 634-638 ◽  
pp. 81-86
Author(s):  
Xiao Hui Zhao ◽  
Qian Hua Zhu ◽  
Qiong Yang ◽  
Wei He ◽  
Shang Zhou ◽  
...  
Keyword(s):  
Light Scattering ◽  
Simultaneous Determination ◽  
Buffer Solution ◽  
Standard Curve ◽  
Scattering Spectrum ◽  
Curve Method ◽  
Standard Curve Method ◽  
Novel Method ◽  
Light Scattering Spectrum

In pH=5.70 B-R buffer solution, Na2WO4 and rhodamine 6G（R6G） could react with natural cytokinins of 6-benzylaminourine (6-BA) and kinetin (KT) and resulted in a great enhacement of synchronous light scattering (SLS) signals. This article first established the degree of polarized synchronous light scattering (P) based on the measurements of the polarized synchronous light scattering signals to distinguish two natural cytokinins of 6-BA and KT. Simultaneously based on the synchronous scattering spectrum and the double standard curve method, a novel method for simultaneous determination of 6-BA and KT was developed by SLS spectralmethod. The method was applied to simultaneous determination of 6-BA and KT in balsam pear skin and tomato skin with satisfactory results.

Atomic Absorption Determination of Nickel in Heavy Crudes via Carbon Rod Atomizer

1977 ◽  
Vol 31 (3) ◽  
pp. 207-210 ◽  
Author(s):  
J. J. Labrecque ◽  
J. Galobardes ◽  
M. E. Cohen
Keyword(s):  
Atomic Absorption ◽  
Standard Curve ◽  
Determination Of Nickel ◽  
Absorption Determination ◽  
Relative Standard ◽  
Curve Method ◽  
Standard Additions ◽  
Standard Curve Method ◽  
Carbon Rod Atomizer

A method is described for the determination of nickel in heavy crude oil by carbon rod atomic absorption spectroscopy in the parts per million range. A 5-g sample is completely dissolved to 25 ml of tetrahydrofuran (THF) stabilized with 0.1% hydroquinone and further diluted with THF. Nickel can be measured at a concentration of 0.1 ppm with a relative standard deviation of about 10% after a 100-fold dilution of the crude. The analysis by standard curve method as well as standard additions methods gave reproducible results. These results also agreed well with values obtained by conventional activation analysis. The method is relatively rapid after sample dilution.

scholarly journals Determination of the Correction Factor for Dinalmefene Hydrochloride in Nalmefene Hydrochloride Injection

2019 ◽  
Vol 267 ◽  
pp. 03001
Author(s):  
Tianyi Zhao ◽  
Ziqing Liu ◽  
Furao Guo ◽  
Peiyu He ◽  
Hongyu Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Correction Factor ◽  
High Performance ◽  
Limit Of Quantification ◽  
Sample Concentration ◽  
Standard Curve ◽  
Curve Method ◽  
Standard Curve Method ◽  
Impurity Detection

Objective: To calculate the detection correction factor for the impurity, that is, the dinalmefene hydrochloride in nalmefene hydrochloride injection. Methods: High performance liquid chromatography (HPLC) is used to analyze the impurities of nalmefene hydrochloride easily produced during storage, and the impurity is determined and correction factor is calculated for the known the dinalmefene hydrochloride. According to the standard curve method, the sample concentration is selected between the detection limit and the limit of quantification, and the standard curve is prepared. The correction factor is then calculated according to the slope of the standard curve. Results: finally, the correction factor for dinalmefene hydrochloride is 0.22. Conclusions: the correction factor calculated by the standard curve method is accurate and reliable, and can be used for impurity detection of nalmefene hydrochloride injection.

A cucurbit[7]uril-based supramolecular fluorescent probe for the detection of metronidazole with high sensitivity and strong anti-interference capacity

2021 ◽  
pp. 174751982110551
Author(s):  
Xuemei Hu ◽  
Huaqing Zhang ◽  
Mei Liu
Keyword(s):  
Fluorescent Probe ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Competitive Reaction ◽  
Standard Curve ◽  
Curve Method ◽  
Standard Curve Method ◽  
Liquid Milk ◽  
High Binding Affinity

We propose a new method for the selective detection of the antibiotic metronidazole (MNZ) using CB[7]-JAT (cucurbit[7]uril = CB[7] and JAT = jatrorrhizine) as a fluorescent probe, which is based on the competitive reaction between MNZ and JAT for the occupancy of the CB[7] cavity. The proposed method gives a good calibration curve in the concentration range of 0.38–60 μM, and the limit of detection for MNZ is 65 ng mL−1 with those obtained by the standard curve method. Moreover, the proposed method was successfully applied for the determination of MNZ in liquid milk. Most importantly, due to the high binding affinity between CB[7] and MNZ, the proposed method shows great anti-interference capacity to accurately detect MNZ in the presence of other antibiotics.

Interferences in the Determination of Titanium in Silicate Rocks and Venezuelan Laterites by Atomic Absorption Spectroscopy

1979 ◽  
Vol 33 (4) ◽  
pp. 389-393 ◽  
Author(s):  
J. J. La Brecque
Keyword(s):  
Atomic Absorption ◽  
Major Elements ◽  
X Ray ◽  
Standard Curve ◽  
Absorption Determination ◽  
Curve Method ◽  
Standard Curve Method ◽  
System 1 ◽  
Interference Studies

A method to overcome the numerous interferences present for the determination of titanium in Venezuelan laterites and bauxites has been adopted. The technique is based on dissolving the sample in the HF-H3BO3 matrix. The advantages of using this system are discussed. After the sample is decomposed in this system, 1 part of 2000 ppm of Al, Si, and Fe in the same system is added to 9 parts of the decomposition solution. The atomic absorption determination is employed using the conventional standard curve method for the 364.3 nm line. A comparison of this method with x-ray fluorescence and colorimetry is given in search of one method for routine batch determinations. Interference studies of aluminium, silicon, and iron, the common major elements in Venezuelan laterites and bauxites, conclude that both aluminum and iron greatly affect the absorption signal for titanium. The proposed method, as well as x-ray fluorescence and colorimetry, was applied to standard reference rocks and a preliminary profile of Venezuelan laterites (ferrugineous bauxites).

scholarly journals ANALYSIS OF TOTAL PHENOLIC FROM A ROOT TABAR KEDAYAN

2019 ◽  
Vol 4 (5) ◽  
pp. 209-214
Author(s):  
Siti Jubaidah ◽  
Henny Nurhasnawati ◽  
Heri Wijaya
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Standard Curve ◽  
East Kalimantan ◽  
Chemical Content ◽  
Curve Method ◽  
Standard Curve Method

East Kalimantan has a diversity of plants from various Dayak ethnic groups. One of them is the Tabar Kedayan (Aristolochia foveolata Merr) plant located in Malinau district. This plant is empirically efficacious as antidiarrhea, antidotum (anti poison). Efficacy of Tabar Kedayan plant has not been scientifically confirmed, this is because there is still little scientific research and information about the content of secondary metabolites and bioactive compounds contained in Tabar Kedayan Root. The presence of secondary metabolite is phenolic which has considerable biological active prospects as antioxidant, antibacterial, antiamuba, anti-inflammatory, antihepatotoxic and antivirus. The aim of this research is to analyze the chemical content and total phenolic content of root tabar kedayan in fractionation with various nonpolar, semipolar and polar solvents. The analysis used in the determination of total phenolic content using visible spectrophotometric method. Data of analysis used standard curve method based on absorbance data and concentration of standard solution. The results of this study obtained the highest total phenolic average on ethyl acetate fraction of 77.74 ±2,633 mg GAE/g then ethanol fraction of water amounted to 38.10 ±0,461 mg GAE/g and the smallest level of n-hexane fraction of 29.36±0,193 mg GAE/g. Conclusions in this study total phenolic content is found in most semipolar fractions.

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