A cucurbit[7]uril-based supramolecular fluorescent probe for the detection of metronidazole with high sensitivity and strong anti-interference capacity

2021 ◽  
pp. 174751982110551
Author(s):  
Xuemei Hu ◽  
Huaqing Zhang ◽  
Mei Liu
Keyword(s):  
Fluorescent Probe ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Competitive Reaction ◽  
Standard Curve ◽  
Curve Method ◽  
Standard Curve Method ◽  
Liquid Milk ◽  
High Binding Affinity

We propose a new method for the selective detection of the antibiotic metronidazole (MNZ) using CB[7]-JAT (cucurbit[7]uril = CB[7] and JAT = jatrorrhizine) as a fluorescent probe, which is based on the competitive reaction between MNZ and JAT for the occupancy of the CB[7] cavity. The proposed method gives a good calibration curve in the concentration range of 0.38–60 μM, and the limit of detection for MNZ is 65 ng mL−1 with those obtained by the standard curve method. Moreover, the proposed method was successfully applied for the determination of MNZ in liquid milk. Most importantly, due to the high binding affinity between CB[7] and MNZ, the proposed method shows great anti-interference capacity to accurately detect MNZ in the presence of other antibiotics.

scholarly journals Ultra violet spectrophotometric analysis of Paracetamol, Diclofenac and Tramadol drugs in mixture

2019 ◽  
Vol 24 (3) ◽  
pp. 52
Author(s):  
Eesa M. Thalij1 ◽  
Sarhan A. Salman2 ◽  
, Hasan. M. Hasan1
Keyword(s):  
Limit Of Detection ◽  
Ultra Violet ◽  
Standard Addition ◽  
Spectrophotometric Analysis ◽  
Standard Curve ◽  
Limit Of Quantitation ◽  
Naoh Solution ◽  
Curve Method ◽  
Standard Curve Method ◽  
Tablet Dosage

The aim of this research was  develop and validate an analytical method by Uv spectrophotometric technique for quantitative determination of paracetamol (PAR), diclofenac or voltarine (DIC) and tramadol or tramal (TRA) in tablet dosage form, paracetamol analysis is based on the absorbance maxima were found to be at 243 nm when dissolved in 0.1N H2SO4 as a sample and in 0.1N NaOH solution as a blank,. Quantitative of PAR in sample is achieved by standard addition methods and three ways calculations were used to estimate the amount in the tablet, the expected content per tablet were equal to  (365.60, 361.984, 358.415 mg) and the results were acceptable when compared with original quantity in tablet(350 mg). The method was compared with standard curved method, showed that it is obeyed Beer – lambert law in the concentration range of 0.1–1 µg/ml for standard addition and standard curve methods, with a correlation coefficient of 0.9980 and o.9944. The limit of detection (LOD) for PAR was 0.036 µg/ml while limit of quantitation (LOQ) 0.119 µg/ml, the recovery of three procedure A, B, C of standard addition and standard curve were (104.40, 103.42, 102.40  and 92.995 %) for PAR ,it was found that the results obtained from the standard addition method were better than the result obtained from the standard curve method. The amount of (DIC) and (TRA) drug in tablet sample calculated depending on the  absorbance (A) at 273 nm to give the value 47.44 mg and 47.6 mg per tablet are acceptable when compared with the value of the original quantity in tablet (50mg) and the recovery of the method was found to be (95.2 and 96.0 % ) respectively, the principle of  the method based on the  (A) of mixture at this λ is a total A of the two drugs, which owns different intensity at this λ at different percentages and that apply to the sample and standard for this drugs. Finally this can applied successfully for routine analysis.    http://dx.doi.org/10.25130/tjps.24.2019.047

Atomic Absorption Determination of Nickel in Heavy Crudes via Carbon Rod Atomizer

1977 ◽  
Vol 31 (3) ◽  
pp. 207-210 ◽  
Author(s):  
J. J. Labrecque ◽  
J. Galobardes ◽  
M. E. Cohen
Keyword(s):  
Atomic Absorption ◽  
Standard Curve ◽  
Determination Of Nickel ◽  
Absorption Determination ◽  
Relative Standard ◽  
Curve Method ◽  
Standard Additions ◽  
Standard Curve Method ◽  
Carbon Rod Atomizer

A method is described for the determination of nickel in heavy crude oil by carbon rod atomic absorption spectroscopy in the parts per million range. A 5-g sample is completely dissolved to 25 ml of tetrahydrofuran (THF) stabilized with 0.1% hydroquinone and further diluted with THF. Nickel can be measured at a concentration of 0.1 ppm with a relative standard deviation of about 10% after a 100-fold dilution of the crude. The analysis by standard curve method as well as standard additions methods gave reproducible results. These results also agreed well with values obtained by conventional activation analysis. The method is relatively rapid after sample dilution.

scholarly journals Determination of the Correction Factor for Dinalmefene Hydrochloride in Nalmefene Hydrochloride Injection

2019 ◽  
Vol 267 ◽  
pp. 03001
Author(s):  
Tianyi Zhao ◽  
Ziqing Liu ◽  
Furao Guo ◽  
Peiyu He ◽  
Hongyu Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Correction Factor ◽  
High Performance ◽  
Limit Of Quantification ◽  
Sample Concentration ◽  
Standard Curve ◽  
Curve Method ◽  
Standard Curve Method ◽  
Impurity Detection

Objective: To calculate the detection correction factor for the impurity, that is, the dinalmefene hydrochloride in nalmefene hydrochloride injection. Methods: High performance liquid chromatography (HPLC) is used to analyze the impurities of nalmefene hydrochloride easily produced during storage, and the impurity is determined and correction factor is calculated for the known the dinalmefene hydrochloride. According to the standard curve method, the sample concentration is selected between the detection limit and the limit of quantification, and the standard curve is prepared. The correction factor is then calculated according to the slope of the standard curve. Results: finally, the correction factor for dinalmefene hydrochloride is 0.22. Conclusions: the correction factor calculated by the standard curve method is accurate and reliable, and can be used for impurity detection of nalmefene hydrochloride injection.

Discrimination and Simultaneous Determination of 6-BA and KT by Polarized Synchronous Light Scattering and Synchronous Light Scattering Spectrum

2013 ◽  
Vol 634-638 ◽  
pp. 81-86
Author(s):  
Xiao Hui Zhao ◽  
Qian Hua Zhu ◽  
Qiong Yang ◽  
Wei He ◽  
Shang Zhou ◽  
...  
Keyword(s):  
Light Scattering ◽  
Simultaneous Determination ◽  
Buffer Solution ◽  
Standard Curve ◽  
Scattering Spectrum ◽  
Curve Method ◽  
Standard Curve Method ◽  
Novel Method ◽  
Light Scattering Spectrum

In pH=5.70 B-R buffer solution, Na2WO4 and rhodamine 6G（R6G） could react with natural cytokinins of 6-benzylaminourine (6-BA) and kinetin (KT) and resulted in a great enhacement of synchronous light scattering (SLS) signals. This article first established the degree of polarized synchronous light scattering (P) based on the measurements of the polarized synchronous light scattering signals to distinguish two natural cytokinins of 6-BA and KT. Simultaneously based on the synchronous scattering spectrum and the double standard curve method, a novel method for simultaneous determination of 6-BA and KT was developed by SLS spectralmethod. The method was applied to simultaneous determination of 6-BA and KT in balsam pear skin and tomato skin with satisfactory results.

Interferences in the Determination of Titanium in Silicate Rocks and Venezuelan Laterites by Atomic Absorption Spectroscopy

1979 ◽  
Vol 33 (4) ◽  
pp. 389-393 ◽  
Author(s):  
J. J. La Brecque
Keyword(s):  
Atomic Absorption ◽  
Major Elements ◽  
X Ray ◽  
Standard Curve ◽  
Absorption Determination ◽  
Curve Method ◽  
Standard Curve Method ◽  
System 1 ◽  
Interference Studies

A method to overcome the numerous interferences present for the determination of titanium in Venezuelan laterites and bauxites has been adopted. The technique is based on dissolving the sample in the HF-H3BO3 matrix. The advantages of using this system are discussed. After the sample is decomposed in this system, 1 part of 2000 ppm of Al, Si, and Fe in the same system is added to 9 parts of the decomposition solution. The atomic absorption determination is employed using the conventional standard curve method for the 364.3 nm line. A comparison of this method with x-ray fluorescence and colorimetry is given in search of one method for routine batch determinations. Interference studies of aluminium, silicon, and iron, the common major elements in Venezuelan laterites and bauxites, conclude that both aluminum and iron greatly affect the absorption signal for titanium. The proposed method, as well as x-ray fluorescence and colorimetry, was applied to standard reference rocks and a preliminary profile of Venezuelan laterites (ferrugineous bauxites).

scholarly journals ANALYSIS OF TOTAL PHENOLIC FROM A ROOT TABAR KEDAYAN

2019 ◽  
Vol 4 (5) ◽  
pp. 209-214
Author(s):  
Siti Jubaidah ◽  
Henny Nurhasnawati ◽  
Heri Wijaya
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Standard Curve ◽  
East Kalimantan ◽  
Chemical Content ◽  
Curve Method ◽  
Standard Curve Method

East Kalimantan has a diversity of plants from various Dayak ethnic groups. One of them is the Tabar Kedayan (Aristolochia foveolata Merr) plant located in Malinau district. This plant is empirically efficacious as antidiarrhea, antidotum (anti poison). Efficacy of Tabar Kedayan plant has not been scientifically confirmed, this is because there is still little scientific research and information about the content of secondary metabolites and bioactive compounds contained in Tabar Kedayan Root. The presence of secondary metabolite is phenolic which has considerable biological active prospects as antioxidant, antibacterial, antiamuba, anti-inflammatory, antihepatotoxic and antivirus. The aim of this research is to analyze the chemical content and total phenolic content of root tabar kedayan in fractionation with various nonpolar, semipolar and polar solvents. The analysis used in the determination of total phenolic content using visible spectrophotometric method. Data of analysis used standard curve method based on absorbance data and concentration of standard solution. The results of this study obtained the highest total phenolic average on ethyl acetate fraction of 77.74 ±2,633 mg GAE/g then ethanol fraction of water amounted to 38.10 ±0,461 mg GAE/g and the smallest level of n-hexane fraction of 29.36±0,193 mg GAE/g. Conclusions in this study total phenolic content is found in most semipolar fractions.

A Comparative Analysis of Methods for Titering Reovirus

2020 ◽  
Vol 8 (5) ◽  
pp. 409-417
Author(s):  
Yi-Chen Yang ◽  
Xian-Yao Wang ◽  
Yuan-Yuan An ◽  
Chun-Xiang Liao ◽  
Nian-Xue Wang ◽  
...  
Keyword(s):  
Traditional Method ◽  
Virus Titer ◽  
Cell Analysis ◽  
Half Cell ◽  
L929 Cells ◽  
Cell Index ◽  
Standard Curve ◽  
Analysis Technique ◽  
Curve Method ◽  
Standard Curve Method

Background: A key challenge in the process of virus amplification is the need for a simple and convenient method for measuring virus titers. Objective: Real-time unlabeled cell analysis (RTCA) was used to establish a standard curve of correlation between half-cell index time (CIT50) and virus titer. At the same time, the virus titer from tunable resistance pulse detection (TRPS) technology was compared with the traditional median tissue culture infectious dose (TCID50) method to evaluate the feasibility and application value of the RTCA technique and TRPS technology. Methods: : Cell index (CI) values for L929 cells under different culture conditions were detected, and the appropriate initial cell inoculation density was screened. The half-cell index (CI50) values of reovirus infected L929 cells with TCID50 titers were analyzed by RTCA, the CI50-TCID50 standard curve was created, and a regression equation was developed. RTCA, TCID50, and TRPS methods were used to detect the reovirus titer obtained by the amplification, and the sensitivity and feasibility of the CIT50-TCID50 standard curve method were analyzed. The virus titer was detected by TRPS technology and the TCID50 method. Results: L929 cells were best propagated at an initial density of 6 × 103 cells/well. After infecting L929 cells with different titers of reference reovirus, the linear correlation of CIT50 and TCID50 was y = -2.1806x + 71.023 (R2 = 0.9742). The titer resulting from the RTCA assay was 7×109.6821 pfu/mL, from the TRPS assay was 4.52×1010 pfu/mL, and from the TCID50 assay was 7×109.467 pfu/mL. Conclusion: The CIT50-TCID50 standard curve method established by the RTCA technique can be used to quantitatively detect reovirus titer with L929 cells. Compared with the TCID50 method, it takes a relatively short time and has high sensitivity and accuracy. The TRPS technology requires even less time to quantify the virus, but its precision is lower than that of the TCID50 method and RTCA technology. This study provides new technical methods for assessing the virulence of infectious live reovirus particles. Lay Summary: After amplification of the virus, we need to detect the virus titers (the virulence of the virus). The traditional method is to use the virus to infect cells, and then the virus titers can be calculated by 50% of the cells infected. However, this traditional method is time consuming. The ways of RTCA (a real-time cell analysis technique) and TRPS (a nano-bioparticle analysis technique) help us to detect viral titers. The consistency of these three methods determines their feasibility and accuracy. If they are feasible, then these two simple technologies will provide new ideas for detecting viral titers.

scholarly journals Copper-nickel oxide nanofilm modified electrode for non-enzymatic determination of glucose

2020 ◽  
Vol 10 (3) ◽  
pp. 245-255
Author(s):  
Mahsa Hasanzadeh ◽  
Zahra Hasanzadeh ◽  
Sakineh Alizadeh ◽  
Mehran Sayadi ◽  
Mojtaba Nasiri Nezhad ◽  
...  
Keyword(s):  
Modified Electrode ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Stability Loss ◽  
The Novel ◽  
Step Potential ◽  
Enzymatic Determination ◽  
Wide Range ◽  
Naoh Solution

CuxO-NiO nanocomposite film for the non-enzymatic determination of glucose was prepared by the novel modifying method. At first, anodized Cu electrode was kept in a mixture solution of CuSO4, NiSO4 and H2SO4 for 15 minutes. Then, a cathodization process with a step potential of -6 V in a mixture solution of CuSO4 and NiSO4 was initiated, generating formation of porous Cu-Ni film on the bare Cu electrode by electrodeposition assisted by the release of hydrogen bubbles acting as soft templates. Optimized conditions were determined by the experimental design software for electrodeposition process. Afterward, Cu-Ni modified electrode was scanned by cyclic voltammetry (CV) method in NaOH solution to convert Cu and Ni nanoparticles to the nano-scaled CuxO-NiO film. The electrocatalytic behavior of the novel CuxO-NiO film toward glucose oxidation was studied by CV and chronoamperometry (CHA) techniques. The calibration curve of glucose was found linear in a wide range of 0.04–5.76 mM, with a low limit of detection (LOD) of 7.3 µM (S/N = 3) and high sensitivity (1.38 mA mM-1 cm-2). The sensor showed high selectivity against some usual interfering species and high stability (loss of only 6.3 % of its performance over one month). The prepared CuxO-NiO nanofilm based sensor was successfully applied for monitoring glucose in human blood serum and urine samples.

scholarly journals Determination of cyanide in bamboo shoots by microdiffusion combined with ion chromatography–pulsed amperometric detection

2018 ◽  
Vol 5 (4) ◽  
pp. 172128 ◽  
Author(s):  
Ming Ding ◽  
Kailiang Wang
Keyword(s):  
Ion Chromatography ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Amperometric Detection ◽  
High Sensitivity ◽  
Practical Method ◽  
Pulsed Amperometric Detection ◽  
Bamboo Shoots ◽  
Relative Standard

A practical method for the determination of cyanide in bamboo shoots has been developed using microdiffusion preparation integrated with ion chromatography–pulsed amperometric detection (IC-PAD). Cyanide was released from bamboo shoots after Conway cell microdiffusion, and then analysed by IC-PAD. In comparison with the previously reported methods, derivatization and ion-pairing agent addition were not required in this proposed microdiffusion combined with IC-PAD method. The microdiffusion parameters were optimized including hydrolysis systems, temperature, time, and so on. Under the optimum conditions, the linear range of the calibration curve for cyanide was 0.2–200.0 µg kg −1 with satisfactory correlation coefficients of 0.9996 and the limit of detection was 0.2 µg kg −1 ( S/N  = 3). The spiked recovery range was from 92.8 to 98.6%. The intra-day and inter-day relative standard deviations of cyanide were 2.7–14.9% and 3.0–18.3%, respectively. This method was proved to be convenient in operation with high sensitivity, precision and accuracy, and was successfully applied in the determination of cyanide in bamboo shoot samples.

scholarly journals An Improved Method for Analysis of Cholecalciferol-Treated Baits

1999 ◽  
Vol 82 (4) ◽  
pp. 792-798
Author(s):  
Richard E Mauldin ◽  
John J Johnston ◽  
Craig A Riekena
Keyword(s):  
Extraction Method ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
Liquid Extraction ◽  
Detector Response ◽  
Improved Method ◽  
Standard Curve ◽  
Time Periods ◽  
Precision And Accuracy

Abstract A liquid extraction method is described that permits rapid determination of cholecalciferol (D3) in rodenticidal grain baits. Purified D3 was incubated for various time periods to produce pre-D3. Response ratios (concentration/detector response) for various concentrations of pre-D3 and D3 in solutions permitted generation of a correction factor for direct quantitation of pre-D3 in solutions with a pure D3 standard. The method has equal precision and accuracy, yet is simpler and less time consuming and requires less solvents than widely accepted methods for extracting D3 from grain baits. Recoveries from control oat baits fortified at 0.05 and 0.75 wt% were 100.9 and 98%, respectively. A standard curve for concentrations ranging from 6.4 to 204 μg/mL had an r2 of 0.9999 and an intercept of zero and was linear and proportional. The method limit of detection was 2.0 × 10−4 wt % D3.

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