The Primary Investigation on Biological Effect of Mesoporous Bioactive Glass In Vivo

2013 ◽  
Vol 750-752 ◽  
pp. 1651-1655
Author(s):  
Bai Yan Sui ◽  
Cheng Tie Wu ◽  
Jiao Sun
Keyword(s):  
Bioactive Glass ◽  
Biological Effect ◽  
Degradation Products ◽  
Long Term Effect ◽  
Liver And Kidney ◽  
Mesoporous Bioactive Glass

Mesoporous bioactive glass (MBG) has superior bioactivity and degradation than non-mesoporous bioactive glass (BG) in vitro. But the biological effect of MBG in vivo is still unknown. In this study, MBG powders with 20μm were implanted into the femoral condyles in SD rats. BG powders with 20μm were used as a control. The local degradation and osteogenesis were observed at 1 week and 4 weeks after implantation, and the systemic toxicity of the degradation products were also evaluated simultaneously. The results revealed MBG powders had the faster rate of degradation and better osteogenesis effect than BG powders at 4 weeks, although the most of material still remained in situ. Histopathological analyses indicated the degradation products did not have any damage to major organs such as liver and kidney. In conclusion, this preliminary study demonstrated that MBG powders have more excellent biological effect at 4 weeks than that of BG in vivo. However the long-term effect needs to be confirmed.

Europium-Containing Mesoporous Bioactive Glass Scaffolds for Stimulating in Vitro and in Vivo Osteogenesis

2016 ◽  
Vol 8 (18) ◽  
pp. 11342-11354 ◽  
Author(s):  
Chengtie Wu ◽  
Lunguo Xia ◽  
Pingping Han ◽  
Lixia Mao ◽  
Jiacheng Wang ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Mesoporous Bioactive Glass

scholarly journals Combination of Selective Etching and Impregnation toward Hollow Mesoporous Bioactive Glass Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1846
Author(s):  
Nurshen Mutlu ◽  
Ana Maria Beltrán ◽  
Qaisar Nawaz ◽  
Martin Michálek ◽  
Aldo R. Boccaccini ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Calcium Nitrate ◽  
Hollow Structure ◽  
Selective Etching ◽  
Bone Tissue Regeneration ◽  
In Vivo Studies ◽  
Spherical Silica Particles ◽  
Mesoporous Bioactive Glass

In this study, binary SiO2-CaO hollow mesoporous bioactive glass nanoparticles (HMBGNs) are prepared by combing selective etching and impregnation strategies. Spherical silica particles (SiO2 NPs) are used as hard cores to assemble cetyltrimethylammonium bromide (CTAB)/silica shells, which are later removed by selective etching to generate a hollow structure. After the removal of CTAB by calcination, the mesoporous shell of particles is formed. Calcium (Ca) is incorporated into the particles using impregnation by soaking the etched SiO2 NPs in calcium nitrate aqueous solution. The amount of incorporated Ca is tailorable by controlling the ratio of SiO2 NPs:calcium nitrate in the soaking solution. The produced HMBGNs are bioactive, as indicated by the rapid formation of hydroxyapatite on their surfaces after immersion in simulated body fluid. In a direct culture with MC3T3-E1 cells, HMBGNs were shown to exhibit concentration-dependent cytotoxicity and can stimulate osteogenic differentiation of MC3T3-E1 cells at concentrations of 1, 0.5, and 0.25 mg/mL. Our results indicate that the combination of selective etching and impregnation is a feasible approach to produce hierarchical HMBGNs. The produced hollow particles have potential in drug delivery and bone tissue regeneration applications, and should be further investigated in detailed in vitro and in vivo studies.

scholarly journals Long-term evolution of antibiotic persistence in P. aeruginosa lung infections

2021 ◽  
Author(s):  
Melanie Ghoul ◽  
Sandra B Andersen ◽  
Helle Krogh Johansen ◽  
Lars Jelsbak ◽  
Søren Molin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Resistance Mechanisms ◽  
Term Evolution ◽  
Bacterial Clone ◽  
Evolution Of Resistance ◽  
Lung Infections

Pathogenic bacteria respond to antibiotic pressure with the evolution of resistance but survival can also depend on their ability to tolerate antibiotic treatment, known as persistence. While a variety of resistance mechanisms and underlying genetics are well characterised in vitro and in vivo, the evolution of persistence, and how it interacts with resistance in situ is less well understood. We assayed for persistence and resistance with three clinically relevant antibiotics: meropenem, ciprofloxacin and tobramycin, in isolates of Pseudomonas aeruginosa from chronic cystic fibrosis lung infections spanning up to forty years of evolution. We find evidence that persistence is under positive selection in the lung and that it can particularly act as an evolutionary stepping stone to resistance. However, this pattern is not universal and depends on the bacterial clone type and antibiotic used, indicating an important role for antibiotic mode of action.

Long-term effect of glucocorticoid on connective tissue of aorta and skin Morphological and biochemical studies of tissues from rabbits with intact and injured aortas

1980 ◽  
Vol 95 (2) ◽  
pp. 271-281 ◽  
Author(s):  
R. Manthorpe ◽  
C. Garbarsch ◽  
I. Lorenzen
Keyword(s):  
Connective Tissue ◽  
Amino Nitrogen ◽  
Vascular Wall ◽  
Term Effect ◽  
Intact Skin ◽  
Long Term Effect ◽  
Biochemical Analyses

Abstract. The long-term effect of prednisolone — 0.6 mg/day for 63 days — upon mechanically induced inflammation and repair processes in vascular connective tissue was compared with that upon undamaged vascular wall and intact skin of rabbits. The investigations included histological examination of aorta as well as biochemical analyses of collagen and various glycosaminoglycan fractions, RNA, DNA and alpha-amino nitrogen. The metabolism of collagen was estimated by in vitro labelling with [14C]proline and the metabolism of glycosaminoglycans by in vivo labelling with [35S]O4. The radioactivity of [125I]albumin in the aorta and serum was also studied. The collagen, glycosaminoglycans, RNA, DNA and water of vascular connective tissue during inflammation and repair and of intact skin was found to be more sensitive to the action of prednisolone than the connective tissue of undamaged vascular wall. An increased degradation of newly synthesized collagen was observed in damaged aorta as well as in skin in which also the biosynthesis of collagen was inhibited. Prednisolone inhibited the biosynthesis of glycosaminoglycans and decreased the total amount of glycosaminoglycans and of nucleic acids in the damaged aortas and the skin. The [125I]albumin aorta-to-serum ratio was significantly increased in the damaged aorta. Prednisolone treatment decreased the ratio in injured aortas, but elevated the ratio in the undamaged vessels. Prednisolone inhibited intimal thickening of the injured aortas.

scholarly journals Acellular Small-Diameter Tissue-Engineered Vascular Grafts

2019 ◽  
Vol 9 (14) ◽  
pp. 2864 ◽  
Author(s):  
Zhen Li ◽  
Xinda Li ◽  
Tao Xu ◽  
Lei Zhang
Keyword(s):  
Effective Means ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Test Results ◽  
In Vivo Test ◽  
Current State

Tissue-engineered vascular grafts (TEVGs) are considered one of the most effective means of fabricating vascular grafts. However, for small-diameter TEVGs, there are ongoing issues regarding long-term patency and limitations related to long-term in vitro culture and immune reactions. The use of acellular TEVG is a more convincing method, which can achieve in situ blood vessel regeneration and better meet clinical needs. This review focuses on the current state of acellular TEVGs based on scaffolds and gives a summary of the methodologies and in vitro/in vivo test results related to acellular TEVGs obtained in recent years. Various strategies for improving the properties of acellular TEVGs are also discussed.

Strontium-incorporated mesoporous bioactive glass scaffolds stimulating in vitro proliferation and differentiation of bone marrow stromal cells and in vivo regeneration of osteoporotic bone defects

2013 ◽  
Vol 1 (41) ◽  
pp. 5711-5722 ◽  
Author(s):  
Yufeng Zhang ◽  
Lingfei Wei ◽  
Jiang Chang ◽  
Richard J. Miron ◽  
Bin Shi ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
In Vitro Proliferation ◽  
Proliferation And Differentiation ◽  
Mesoporous Bioactive Glass

Sr-containing mesoporous bioactive glass scaffolds significantly enhanced the regeneration of osteoporotic bone defects.

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