Detection and Analysis of Intracellular Polymers Stored by Polyphosphate-Accumulating Microorganisms
Keyword(s):
Both qualitative and quantitative analysis of intracellular polymers in polyphosphate accumulating organisms (PAOs) was discussed in this paper. Results were indicated that black-blue particles of poly-β-hydroxyalkanoate (PHBs) were observed clearly by Sudan black staining. DAPI staining and the Neisser new staining for polyphosphate (poly-P) were more proper than other staining methods. PHB content reached 198.3 mg/g MLSS detected by GC. After digested by HClO4, the poly-P content of sludge was determined to be 138.4 mg/g MLSS.
2004 ◽
2017 ◽
Vol 4
(3)
◽
pp. 01