Effect of Nitrate on Inducement of Denitrifying Phosphate-Accumulating Organisms

2013 ◽  
Vol 864-867 ◽  
pp. 201-204
Author(s):  
Li Qing Zhang ◽  
Gang Zhang

Denitrifying phosphate accumulating (DNPA) was investigated by controlling the concentration of nitrate in anoxic stage with batch experiments. A linear relationship between the quantity of phosphorus uptaken and the consumption of nitrate was shown under anoxic condition with the same concentration of carbon source. Based on the slope of the line between phosphorus release quantity and nitrate consumption, nitrate concentration was adjusted in the anoxic stage. Excellent DNPA performance had been achieved rapidly. The removal efficiency of phosphorus was above 95%, and nitrate more than 96%. According to the maximum phosphorus uptake rate of anoxic or aerobic, it was calculated that the percent of DNPAOs to PAOs was increased from 22% to 79% after cultivation.

2004 ◽  
Vol 50 (10) ◽  
pp. 153-161 ◽  
Author(s):  
Y.Y. Wang ◽  
Y.Z. Peng ◽  
C.Y. Peng ◽  
S.Y. Wang ◽  
W. Zeng

The effect of added carbon source and nitrate concentration on the denitrifying phosphorus removal by denitrifying phosphorus removal bacteria sludge was systematically studied using batch experiments, at the same time the variation of ORP was investigated. Results showed that the denitrifying and phosphorus uptake rate in the anoxic phase increased with the high initial anaerobic carbon source addition. However, once the initial COD concentration reached a certain level, which was in excess of the PHB saturation of Poly-p bacteria, residual COD carried over to the anoxic phase inhibited the subsequent denitrifying phosphorus uptake. This was equal to supplementing the external carbon source to the anoxic phase, furthermore the higher the external carbon source concentration the more powerful the inhibition caused. High nitrate concentration in the anoxic phase increased the initial denitrifying phosphorus rate. Once the nitrate was exhausted, phosphate uptake changed to phosphate release. Moreover, the time of this turning point occurred later with the higher nitrate addition. On the other hand, through on-line monitoring the variation of the ORP with different initial COD concentration, it was found that ORP could be used as a control parameter for phosphorus release, but it is impossible to utilize ORP for controlling the dinitrification and anoxic phosphorus uptake operations.


2010 ◽  
Vol 113-116 ◽  
pp. 2201-2207 ◽  
Author(s):  
Jun Yin ◽  
Lei Wu ◽  
Ke Zhao ◽  
Yu Juan Yu

In this article, analysis the start-up of A2/O humic activated sludge system phosphorus removal efficiency and the characteristics of anaerobic phosphorus release, aerobic phosphorus uptake, sludge activity and their change in the Series Technologies process. The results show that A2/O humic activated sludge system phosphorus removal rate stabilized at 90.7% ~ 97.6%. Sludge activity except for anoxic zone 2 increased, along the process showed a gradual decrease trend.


2013 ◽  
Vol 726-731 ◽  
pp. 2156-2159 ◽  
Author(s):  
Jing Ni Xiao ◽  
Li Na Zheng ◽  
Lei Zhang ◽  
Han Min Zhang ◽  
Feng Lin Yang

The phosphorus uptake rate characteristics have been investigated in different electron acceptor conditions (NO3-, O2, O2 and NO3- coexisting). The sludge was transferred from CAS, AO MBR, AOA MBR, A2O MBR or the A, B tank of MUCT-MBR systems. The results show that the phosphorus uptake rate (SPUR) have the same rule for the sludge in different electron acceptors, that is NSPUR (NO3- as the electron acceptor) <ASPUR (O2 as the electron acceptor) <TSPUR (both O2 and NO3- as the electron acceptors). There exists the aerobic denitrifying phosphorus removal process in mixed electron acceptor system. And this process shows a positive correlation with the ability of denitrifying phosphorus removal improved. The higher ability of denitrifying phosphorus uptake the sludge have, the faster nitrate consumption rate could be shown through the phosphorus uptake process under the condition of in O2 and NO3- coexisting. That is TSDNR(AO)<TSDNR(AOA)<TSDNR(A2O). The relationship between the nitrate consumption rates is ASDNR (only O2 exist) <TSDNR (TP and O2 coexist) <NSDNR (only TP exist). The phosphorus removal mechanism might include three parts: the aerobic phosphorus uptake, the anoxic denitrifying phosphorus uptake and the aerobic denitrifying phosphorus uptake.


2013 ◽  
Vol 860-863 ◽  
pp. 506-509
Author(s):  
Zhen Zhou ◽  
Wei Min Qiao ◽  
Rezihan Muhemaiti ◽  
Zhen Li ◽  
Ling Yan Jiang ◽  
...  

Effects of primary settling tank (PST) on phosphorus removal and the ratio of denitrifying phosphorus removing bacteria (DPB) to phosphorus accumulating organisms (PAOs) in a full-scale anaerobic/anoxic/aerobic (AAO) process were investigated by field survey and batch test. The AAO process without PST showed higher phosphorus removal efficiency than that with PST. In the anoxic tank, the phenomenon of anoxic phosphorus uptake and anaerobic phosphorus release were observed in the AAO process without and with PST, respectively. The DBP/PAOs ratio was 46% and 39% in the AAO process with and without PST, respectively. The AAO process with PST yielded higher microbial activities than that without PST.


1998 ◽  
Vol 38 (1) ◽  
pp. 107-114 ◽  
Author(s):  
S. H. Chuang ◽  
C. F. Ouyang ◽  
H. C. Yuang ◽  
S. J. You

Accumulating and utilizing PHAs (polyhydroxyalkanoates), i.e. a major carbon reserve of polyphosphate accumulating organisms (PAOs), is a prerequisite for phosphorus removal in an enhanced biological phosphorus removal (EBPR) system. To evaluate phosphorus removal, this study investigates the behavior of PHAs in a hybrid anaerobic-anoxic-aerobic process, operating under various sludge retention times (5, 10, 12 and 15 days) and dissolved oxygen conditions (0.5, 1.0 and 2.0 mg/l in aerobic stage). PHAs and phosphorus measurements in the pilot-scale experiments demonstrate that the PHAs content of sludge closely relates to phosphorus release and uptake behavios under anaerobic and aerobic conditions, respectively. The aerobic specific-phosphorus-uptake-rate is directly proportional to PHAs content of sludge in the anoxic stage. When the process is under a high organic loading condition, the sludge exhibits a large amount of PHAs having accumulated in the anoxic stage and a high phosphorus uptake rate in the subsequent aerobic stage. However, experimental results confirm that anoxic phosphorus release, leading to a high concentration of phosphorus flow into the aerobic stage, causes deficient phosphorus removal under a high organic loading condition. Moreover, a low PHAs content of sludge causes incomplete phosphorus removal; the phenomenon occurs when the process is under a low organic loading condition. Based on the results presented herein, we can conclude that the organic loading should be carefully controlled for phosphorus removal in the anaerobic-anoxic-aerobic process.


2011 ◽  
Vol 383-390 ◽  
pp. 3707-3712
Author(s):  
L M Yuan ◽  
C Y Zhang ◽  
R Yan ◽  
G Z Zhao ◽  
L J Tian ◽  
...  

An innovative step-feed Anaerobic-multiple Anoxic/Oxic-Membrane Bioreactor [An-M(A/O-MBR] process was developed to treat synthetic domestic wastewater. Performance of the lab-scale system was investigated at different dissolved oxygen (DO) concentration (0.4-2.4mg/L) in the aerobic tank of the multiple A/O zone. The results showed that, under the conditions imposed, the DO level has no obvious influence on COD removal and the removal efficiency was more than 94% throughout the operation. However, DO levels have great influence on nitrogen and phosphorus removal. Better nitrification performance(>99%) could be obtained when DO was in the range of 0.8~2.4mg/L. High total nitrogen (TN) and total phosphorus (TP) removal performance can be obtained when DO was in the range of 0.8~1.2mg/L, the average removal efficiency was 74.81% and 71.41%, respectively. In this condition, DO can meet the demand of nitrification and phosphorus uptake simultaneously, and the simultaneous nitrification and denitrification (SND) occurred in the aerobic tanks under 1ower DO concentration. Meanwhile, since the DO circulated from the aerobic tank to the anoxic tank decreased, denitrification was enhanced and the nitrate quantity in the sludge recycle system decreased, resulting in the decrease of carbon substrate competition between denitrifcation and phosphorus release in the anaerobic zone. Ultimately, the performance of TN and TP removal was enhanced.


2013 ◽  
Vol 864-867 ◽  
pp. 1886-1889
Author(s):  
Xiao Yan Tian ◽  
Liang Wang

Based on An / OSBR, the paper explores the different sludge loading and nitrate concentrations under the conditions of An / OSBR effects of biological phosphorus removal systems.The influent phosphorus concentration constant at 10 ± 0.5mg / L, in order to compare the end of the anaerobic and effluent TP concentration.The results showed that: ①With the increasing of nitrate concentrations , PAOs release phosphorus in the anaerobic reaction zone is decreased, nitrate appears to reduce the synthesis of PHB, which is due to PHB synthesis and denitrification requires a simple carbon source as hydrogen donor, resulting in competition;② With the COD sludge load increases, the effluent TP concentration decreased, when the COD sludge load ≥ 0.46mgCOD/mgMLSS · d, the effluent TP concentrations is less than 1mg / L;③ When the reaction temperature was 25 °C and 15 °C , the releasing phosphorus in anaerobic and COD sludge load have a good correlation,Linear relationship between the two were: y = 0.72 +71.91 x ( 25 °C), y = 2.81 +73.33 x (15 °C);


2014 ◽  
Vol 955-959 ◽  
pp. 231-234
Author(s):  
Hui Yang ◽  
Ji Gang Yang ◽  
Meng Zhao

This paper aims to study the impact of different carbon sources on phosphorus removal after the (AO)2SBR system was started successfully. Four kinds of carbon resources were used in sequence experiments to observe the impact of different carbon resources on phosphorus release at anaerobic condition, uptake aerobic condition, uptake anoxic condition and phosphorus removal of the (AO)2SBR system. And the carbon sources are sodium acetate, sodium propionate, glucose, sodium acetate + sodium propionate separately. The experiment result shows that the phosphorus release and uptake of active sludge at anaerobic/aerobic/anoxic condition was preferable with sodium acetate as carbon source. And the phosphorus removal rate of (AO)2SBR system and TP concentration in the effluent were 95% and under 0.5mg·L-1 respectively. The phosphorus removal rate of (AO)2SBR system was only 60% with glucose as carbon resource. This study concluded that it is beneficial for the biological phosphorus removal to choose organics with short carbon chain, such as sodium acetate as carbon source.


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