Electrochemical Detection of Bacteria Using Graphene Oxide Electrodeposited on Titanium Implants

2014 ◽  
Vol 96 ◽  
pp. 45-53 ◽  
Author(s):  
Sirinrath Sirivisoot ◽  
Yardnapar Parcharoen ◽  
Thomas J. Webster
Keyword(s):  
Electron Transfer ◽  
Graphene Oxide ◽  
Electrochemical Sensors ◽  
Direct Electron Transfer ◽  
Orthopedic Implants ◽  
Titanium Implants ◽  
Cyclic Voltammograms ◽  
Label Free ◽  
Bacteria Growth ◽  
E Coli

Graphene oxide was electrodeposited on titanium (Ti-GO) and anodized titanium (ATi-GO) as label-free sensors for the detection of challenging living organisms, specificallyEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus). The graphene modification contributed to two sets of oxidation-reduction peaks in cyclic voltammograms (CVs) of bacteria growth on the electrode surfaces (ATi-GO) that resulted in increasing direct electron transfer and stimulating excretion of mediating molecules for higher electron transfer between electrodes and bacteria. Additionally, similar wave patterns of CVs were found whenE. coliorS. aureuswere grown and electrocatalyzed on ATi-GO. The results suggest that bacteria on titanium implant surfaces could be easily detected by using mediatorless ATi-GO sensors electrochemically. These finding open another interesting method in using ATi-GO asin situelectrochemical sensors for label-free, close to real-time detection of bacteria infection in orthopedic implants.

Direct electron transfer in E. coli catalyzed MFC with a magnetite/MWCNT modified anode

RSC Advances ◽  
2013 ◽  
Vol 3 (37) ◽  
pp. 16665 ◽  
Author(s):  
In Ho Park ◽  
Yoon Hye Heo ◽  
Pil Kim ◽  
Kee Suk Nahm
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
E Coli

Direct electrochemical deposition of polyaniline nanowire array on reduced graphene oxide modified graphite electrode for direct electron transfer biocatalysis

RSC Advances ◽  
2015 ◽  
Vol 5 (113) ◽  
pp. 93209-93214 ◽  
Author(s):  
Lin Xia ◽  
Jianfei Xia ◽  
Zonghua Wang
Keyword(s):  
Electron Transfer ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Electrochemical Deposition ◽  
Graphite Electrode ◽  
Direct Electron Transfer ◽  
Nanowire Array ◽  
Graphite Electrodes ◽  
Reduced Graphene

Direct electron transfer biocatalysis was achieved via electrochemically produced ordered PANI nanowire array on reduced graphene oxide modified graphite electrodes.

A label-free and sensitive electrochemical aptasensor for thrombin based on the direct electron transfer of hemin and hemin@rGO nanosheets as the signal probe

2015 ◽  
Vol 7 (20) ◽  
pp. 8771-8777 ◽  
Author(s):  
Shuyan Xue ◽  
Huafyu Yi ◽  
Yali Yuan ◽  
Pei Jing ◽  
Wenju Xu
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Sensitive Detection ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Signal Probe

In this work, a label-free electrochemical aptasensor for sensitive detection of thrombin was fabricated and characterized.

scholarly journals Hyaluronate-Functionalized Graphene for Label-Free Electrochemical Cytosensing

Micromachines ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 669 ◽  
Author(s):  
Aihua Jing ◽  
Chunxin Zhang ◽  
Gaofeng Liang ◽  
Wenpo Feng ◽  
Zhengshan Tian ◽  
...  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Cancer Cells ◽  
Electrochemical Sensors ◽  
Electrochemical Impedance ◽  
Cyclic Voltammograms ◽  
Cell Detection ◽  
Functionalized Graphene ◽  
Label Free ◽  
Promising Alternative

Electrochemical sensors for early tumor cell detection are currently an important area of research, as this special region directly improves the efficiency of cancer treatment. Functional graphene is a promising alternative for selective recognition and capture of target cancer cells. In our work, an effective cytosensor of hyaluronate-functionalized graphene (HG) was prepared through chemical reduction of graphene oxide. The as-prepared HG nanostructures were characterized with Fourier transform infrared spectroscopy and transmission electron microscopy coupled with cyclic voltammograms and electrochemical impedance spectroscopy, respectively. The self-assembly of HG with ethylene diamine, followed by sodium hyaluronate, enabled the fabrication of a label-free electrochemical impedance spectroscopy cytosensor with high stability and biocompatibility. Finally, the proposed cytosensor exhibited satisfying electrochemical behavior and cell-capture capacity for human colorectal cancer cells HCT-116, and also displayed a wide linear range, from 5.0 × 102 cells∙mL−1 to 5.0 × 106 cells∙mL−1, and a low detection limit of 100 cells∙mL−1 (S/N = 3) for quantification. This work paves the way for graphene applications in electrochemical cytosensing and other bioassays.

scholarly journals Novel Composite Electrode of the Reduced Graphene Oxide Nanosheets with Gold Nanoparticles Modified by Glucose Oxidase for Electrochemical Reactions

Catalysts ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 764 ◽  
Author(s):  
Dong ◽  
Yu ◽  
Liu ◽  
Liu ◽  
Shao ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Direct Electron Transfer ◽  
Cyclic Voltammograms ◽  
Graphene Oxide Nanosheets ◽  
X Ray ◽  
Reduced Graphene

Graphene-based composites have been widely explored for electrode and electrocatalyst materials for electrochemical energy systems. In this paper, a novel composite material of the reduced graphene oxide nanosheets (rGON) with gold nanoparticles (NPs) (rGON-AuNP) is synthesized, and its morphology, structure, and composition are characterized by scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), energy dispersive X-ray (EDX), Fourier transform infrared spectroscopic (FTIR), Raman, and UV-Vis techniques. To confirm this material’s electrochemical activity, a glucose oxidase (GOD) is chosen as the target reagent to modify the rGON-AuNP layer to form GOD/rGON-AuNP/glassy carbon (GC) electrode. Two pairs of distinguishable redox peaks, corresponding to the redox processes of two different conformational GOD on AuNP, are observed on the cyclic voltammograms of GOD/rGON-AuNP/GC electrode. Both cyclic voltammetry and electrochemical impedance spectroscopy are employed to study the mechanism of direct electron transfer from GOD to GC electrode on the rGON-AuNP layer. In addition, this GOD/rGON-AuNP/GC electrode shows catalytic activity toward glucose oxidation reaction.

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