Collagen-Chitosan- Glycerol-HPMC Composite as Cornea Artificial Candidate

2019 ◽  
Vol 42 ◽  
pp. 14-21
Author(s):  
Prihartini Widiyanti ◽  
Reni Prastyani
Keyword(s):  
Pore Size ◽  
Host Response ◽  
Living Cells ◽  
Cytotoxicity Test ◽  
Artificial Cornea ◽  
Functional Cluster ◽  
Post Surgery ◽  
Cornea Transplantation ◽  
Simplex Virus

The number of blindness is tend to be increased year by year. One of the blindness cause is cornea ulcer.The cause of cornea ulcer is bacteria, fungi, and herpes simplex virus. Cornea transplantation is the only treatment which could widely accepted for blindness. Transplant by donor network becomes the only treatment that is acceptable on a large for blindness. However, treatment donor transplants have many shortcomings in complications post surgery such as host response, donor limitations, incompatibility and the length of time healing. As technology develops, there are many corneal substitutes based on natural ingredients derived from collagen or their derivatives because they promise better properties in biocompatibility. The aim of research are to conduct the synthesis and characterization of collagen- chitosan- glycerol - HPMC as artificial cornea such functional cluster test, cytotoxycity test, morphological test and antibacterial test. Based on functional cluster test, there are functional groups of all components of composite materials. While from cytotoxicity test, all samples have a percentage of living cells above 85%. The morphology test is showed that the pore size of sample B with composition collagen-chitosan-glycerol-HPMC is in accordance with the standard pore size for keratoprothesis. Sample A (collagen-chitosan-glycerol) and sample B (collagen-chitosan-glycerol-HPMC) have strong antibacterial properties.Biocomposite of collagen-chitosan-glycerol could be considered as artificial cornea due to the proximity with the corneal characteristics.

CHARACTERIZATION OF PORE SIZE DISTRIBUTIONS USING NON-NEWTONIAN FLUIDS

2020 ◽  
Author(s):  
Scott C. Hauswirth ◽  
◽  
Majdi Abou Najm ◽  
Christelle Basset
Keyword(s):  
Pore Size ◽  
Newtonian Fluids ◽  
Size Distributions ◽  
Pore Size Distributions

scholarly journals Real-Time Challenging of ERα Y537S Mutant Transcriptional Activity in Living Cells

Endocrines ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 54-64
Author(s):  
Manuela Cipolletti ◽  
Sara Pescatori ◽  
Filippo Acconcia
Keyword(s):  
Cell Line ◽  
Transcriptional Activity ◽  
Estrogen Receptor Α ◽  
Living Cells ◽  
Patient Treatment ◽  
Estrogen Responsive Element ◽  
Specific Proteins ◽  
Responsive Element ◽  
Mcf 7

Metastatic estrogen receptor α (ERα)-expressing breast cancer (BC) occurs after prolonged patient treatment with endocrine therapy (ET) (e.g., aromatase inhibitors—AI; 4OH-tamoxifen—4OH-Tam). Often these metastatic BCs express a mutated ERα variant (e.g., Y537S), which is transcriptionally hyperactive, sustains uncontrolled proliferation, and renders tumor cells insensitive to ET drugs. Therefore, new molecules blocking hyperactive Y537S ERα mutation transcriptional activity are requested. Here we generated an MCF-7 cell line expressing the Y537S ERα mutation stably expressing an estrogen-responsive element (ERE) promoter, which activity can be monitored in living cells. Characterization of this cell line shows both hyperactive basal transcriptional activity with respect to normal MCF-7 cells, which stably express the same ERE-based promoter and a decreased effect of selective ER downregulators (SERDs) in reducing Y537S ERα mutant transcriptional activity with respect to wild type ERα transcriptional activity. Kinetic profiles of Y537S ERα mutant-based transcription produced by both drugs inducing receptor degradation and siRNA-mediated depletion of specific proteins (e.g., FOXA1 and caveolin1) reveals biphasic dynamics of the inhibition of the receptor-regulated transcriptional effects. Overall, we report a new model where to study the behavior of the Y537S ERα mutant that can be used for the identification of new targets and pathways regulating the Y537S ERα transcriptional activity.

scholarly journals Characterization of Herpes Simplex Virus-induced Deoxyribonucleic Acid Polymerase

1973 ◽  
Vol 248 (18) ◽  
pp. 6270-6277
Author(s):  
Arthur Weissbach ◽  
Su-Chen L. Hong ◽  
Jayme Aucker ◽  
Robert Muller
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Deoxyribonucleic Acid ◽  
Simplex Virus

scholarly journals De-Coding the Contributions of the Viral RNAs to Alphaviral Pathogenesis

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 771
Author(s):  
Autumn T. LaPointe ◽  
Kevin J. Sokoloski
Keyword(s):  
Host Response ◽  
Severe Disease ◽  
Review Article ◽  
Structural Proteins ◽  
Virulence Determinants ◽  
Healthy Individuals ◽  
Viral Rnas ◽  
Positive Sense ◽  
Identification And Characterization

Alphaviruses are positive-sense RNA arboviruses that are capable of causing severe disease in otherwise healthy individuals. There are many aspects of viral infection that determine pathogenesis and major efforts regarding the identification and characterization of virulence determinants have largely focused on the roles of the nonstructural and structural proteins. Nonetheless, the viral RNAs of the alphaviruses themselves play important roles in regard to virulence and pathogenesis. In particular, many sequences and secondary structures within the viral RNAs play an important part in the development of disease and may be considered important determinants of virulence. In this review article, we summarize the known RNA-based virulence traits and host:RNA interactions that influence alphaviral pathogenesis for each of the viral RNA species produced during infection. Overall, the viral RNAs produced during infection are important contributors to alphaviral pathogenesis and more research is needed to fully understand how each RNA species impacts the host response to infection as well as the development of disease.

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