Cytotoxicity Evaluation of Bioactive Glass-Polyvinyl Alcohol Hybrid Foams Prepared by the Sol-Gel Method

2005 ◽  
Vol 284-286 ◽  
pp. 589-592 ◽  
Author(s):  
Marivalda Pereira ◽  
Najat Al-Saffar ◽  
Jamuna Selvakumaran ◽  
Larry L. Hench
Keyword(s):  
Tissue Engineering ◽  
Polyvinyl Alcohol ◽  
Cell Viability ◽  
Bioactive Glass ◽  
Pore Structure ◽  
Sol Gel ◽  
Cleaning Procedure ◽  
Gel Method ◽  
Cleaning Methods ◽  
Hybrid Foams

Hybrid bioactive glass-polyvinyl alcohol foams for use as scaffolds in tissue engineering were developed through the sol-gel route. Hybrids produced by this route present a high acidic character due to the catalysts added during processing and may also contain residual organics after the drying step. Therefore, an additional cleaning step is necessary to produce biocompatible materials. In this study hybrid PVA/bioactive glass foams were cleaned using various procedures and cytotoxicity evaluation was conducted. All the cleaning methods used increased the cell viability levels compared to samples not subjected to a cleaning procedure. The most effective cleaning procedure used was the immersion in NH4OH solution. The cleaning procedure changed the composition and pore structure of the final material.

Preparation of bioactive glass-polyvinyl alcohol hybrid foams by the sol-gel method

2005 ◽  
Vol 16 (11) ◽  
pp. 1045-1050 ◽  
Author(s):  
M. M. Pereira ◽  
J. R. Jones ◽  
R. L. Orefice ◽  
L. L. Hench
Keyword(s):  
Polyvinyl Alcohol ◽  
Bioactive Glass ◽  
Sol Gel ◽  
Gel Method ◽  
Sol Gel Method ◽  
Hybrid Foams

Mechanical Behavior of Bioactive Glass-Polyvinyl Alcohol Hybrid Foams Obtained by the Sol-Gel Process

2005 ◽  
Vol 284-286 ◽  
pp. 757-760 ◽  
Author(s):  
Marivalda Pereira ◽  
Showan N. Nazhat ◽  
Julian R. Jones ◽  
Larry L. Hench
Keyword(s):  
Polyvinyl Alcohol ◽  
Bioactive Glass ◽  
Mechanical Behavior ◽  
Sol Gel ◽  
Precursor Solution ◽  
High Porosity ◽  
Soft Tissue Regeneration ◽  
Sol Gel Process ◽  
Potential Applications ◽  
Hybrid Foams

The possibility of enhancing mechanical properties by incorporation of polymeric components to sol-gel derived materials is extremely attractive to prepare macroporous scaffolds, leading to materials with potential applications in both hard and soft tissue regeneration. In this work bioactive glass-polyvinyl alcohol hybrids were developed and their mechanical behavior was evaluated. Hybrids were synthesized by adding polyvinyl alcohol to a sol-gel precursor solution, which was then foamed with the addition of a surfactant and vigorous agitation. The foams were cast, aged and dried at 40°C. A cleaning step to decrease the acidic character of the obtained hybrids was undertaken by immersion in a NH4OH solution. The mechanical behavior of the hybrids was evaluated in compression using both stress and strain control tests. Hybrid foams had a high porosity varying from 60-90% and the macropore diameter ranged from 10 to 600 µm. The modal macropore diameter varied with the inorganic phase composition and with the polymer content in the hybrid. The strain at fracture of the as prepared hybrid foams was substantially greater than pure gel-glass foams. The cleaned hybrids presented a slightly higher strength and lower deformation than the as prepared foams.

Acid character control of bioactive glass/polyvinyl alcohol hybrid foams produced by sol–gel

2008 ◽  
Vol 47 (3) ◽  
pp. 335-346 ◽  
Author(s):  
A. A. R. de Oliveira ◽  
V. Ciminelli ◽  
M. S. S. Dantas ◽  
H. S. Mansur ◽  
M. M. Pereira
Keyword(s):  
Polyvinyl Alcohol ◽  
Bioactive Glass ◽  
Sol Gel ◽  
Hybrid Foams ◽  
Acid Character

scholarly journals Comparison of the Effect of Sol-Gel and Coprecipitation Routes on the Properties and Behavior of Nanocomposite Chitosan-Bioactive Glass Membranes for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Elke M. F. Lemos ◽  
Sandhra M. Carvalho ◽  
Patrícia S. O. Patrício ◽  
Claudio L. Donnici ◽  
Marivalda M. Pereira
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Sol Gel ◽  
Composite Films ◽  
Maximum Tensile Stress ◽  
Control Group ◽  
Nanoparticle Dispersion

Recent studies in tissue engineering have highlighted the importance of the development of composite materials based on biodegradable polymers containing bioactive glasses, in particular, composites for high load support and excellent cell viability for potential application in bone regeneration. In this work, hybrid composite films were obtained by combining chitosan with bioactive glass in solution form and in nanoparticle dispersion form obtained by the two different synthesis routes: the sol-gel method and coprecipitation. The bioactive glass served both as a mechanical reinforcing agent and as a triggering agent with high bioactivity. The results ofin vitroassays with simulated body fluid demonstrated the formation of a significant layer of fibrils on the surface of the film, with a typical morphology of carbonated hydroxyapatite, reflecting induction of a favorable bioactivity. Maximum tensile stress increased from 42 to 80 MPa to the sample with 5% wt bioactive glass. In addition, samples containing 5% and 10% wt bioactive glass showed a significant increase in cell viability, 18 and 30% increase compared to the control group. The samples showed significant response, indicating that they could be a potential material for use in bone regeneration through tissue engineering.

Effect of Increasing Polyvinyl Alcohol Content on the Porous Structure and Mechanical Properties of Sol-Gel Derived Hybrids Foams

2007 ◽  
Vol 361-363 ◽  
pp. 555-558 ◽  
Author(s):  
Agda Aline Rocha de Oliveira ◽  
R.L. Oréfice ◽  
Herman S. Mansur ◽  
Marivalda Pereira
Keyword(s):  
Mechanical Properties ◽  
Polyvinyl Alcohol ◽  
Bioactive Glass ◽  
Structural Characteristics ◽  
Sol Gel ◽  
Acetate Solution ◽  
Compression Tests ◽  
Sol Gel Process ◽  
Hybrid Foams

Bioactive glass/polymer hybrids are promising materials for biomedical applications because they combine the bioactivity of these bioceramics with the flexibility of polymers. In previous work hybrid foams with 80% bioactive glass and 20% polyvinyl alcohol (PVA) were prepared by the sol-gel process for application as scaffold for bone tissue engineering. In this work it was evaluated the effect of increasing the PVA content of the hybrids on structural characteristics and mechanical properties of hybrid foams produced by this method. The hybrids were prepared with inorganic phase composition of 70%SiO2-30%CaO and PVA fractions of 20 to 60% by the sol-gel method. The structural and mechanical characterization of the obtained foams was done by FTIR, SEM, Helium Picnometry, and compression tests. To reduce the acidic character of the hybrids due to the catalysts added, different neutralization solutions were tested. The immersion of hybrids in a calcium acetate solution was the most adequate neutralization method, avoiding calcium loss while maintaining pH nearly 7,0 and low PVA loss. The foams presented porosity of 60-85% and pore diameters of 100-500μm with interconnected structure. The pore structure varied with the polymer content in the hybrid. The compression tests showed that an increase of PVA fraction in the hybrids improved their mechanical properties.

scholarly journals Attachment and Proliferation of Human-Adipose-Tissue-Derived Stem Cells on Bioactive Glass/PVA Hybrid Scaffolds

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Viviane Gomide ◽  
Alessandra Arcoverde Cavalcanti Zonari ◽  
Natalia Martins Breyner ◽  
Alfredo Miranda de Goes ◽  
Marivalda M. Pereira
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Bioactive Glass ◽  
Pore Structure ◽  
Bone Repair ◽  
Sol Gel ◽  
Human Adipose Tissue ◽  
Porous Scaffolds

Bioactive glass/polymer hybrids are promising materials for biomedical applications because they combine the bioactivity of bioceramics with the flexibility of polymers. These advantages may be used in porous scaffolds for tissue-engineering applications. In previous works, hybrid foams bioactive glass/polyvinyl alcohol (PVA) were prepared by the sol-gel process. The hybrid foam 50% PVA/50% bioactive glass was chosen in the composition range studied as the scaffold with better compromise in terms of pore structure and mechanical behavior. The aim of the present study was to evaluate the adhesion, viability, and growth behavior of human-adipose-tissue-derived stem cells on bioactive glass/PVA foams in vitro and their potential as scaffold for application in bone-tissue engineering. The pore structure of the hybrid samples used in the study was analyzed by microcomputed tomography, showing a modal pore diameter of 284 μm and modal interconnect diameter of 138 μm. We found that cells are capable of adhesion, proliferation, growth, and ECM production on the scaffolds tested. The results show that the hybrid bioactive glass/PVA is a promising material for bone repair, providing a good environment for the adhesion and proliferation of human-adipose-tissue-derived stem cells in vitro.

scholarly journals Study Structural properties of Nanocomposite (polyvinyl Alcohol\ (CdO/NiO) via Sol-Gel Method

2021 ◽  
Author(s):  
Amatalkareem Mohammed Al-Jezbi
Keyword(s):  
Polyvinyl Alcohol ◽  
Structural Properties ◽  
Sol Gel ◽  
Cadmium Oxide ◽  
Antibacterial Activities ◽  
Oxide Mixture ◽  
Gel Method ◽  
Sol Gel Method ◽  
Diffraction Patterns ◽  
Cracking Process

Abstract This study deals with the synthesis of some physical properties of (CdO/NiO) nanocomposite via Sol-Gel method and their antibacterial activities. The NiO and CdO nanoxides as well as the CdO/NiO oxide mixture were prepared in five samples with different concentrations where the ratio of CdO/NiO was [(1: 0), (3: 1), (1: 1), (1: 3), (0: 1)], by using the gel solution by means of a polyvinyl alcohol solution ( PVA ). The samples were platelimb at (500̊C) for three hours. The structural properties of the prepared oxides resulting from the cracking process were studied. The XRD results showed the presence of pure cadmium oxide diffraction patterns in the sample (S 1 ) as well as pure nickel oxide in the sample (S 5 ), and confirmed the presence of the CdO / NiO oxide mixture in the samples (S 2 , S 3 , S 4 ). The results of XRD also showed, through calculations, that the specific surface area increased with increasing granular size and decreased with decreasing granular size. XRD calculations also showed that the intensity of eruptions decreased as the granular volume increased and, inversely, increased with decreasing granular size.

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