Confocal Study of Specific Targeting of Quantum Dot Nanocomposites to Cancer Cells

2005 ◽  
Vol 288-289 ◽  
pp. 155-158 ◽  
Author(s):  
Yong Zhang ◽  
J. Zhang
Keyword(s):  
Quantum Dots ◽  
Optical Properties ◽  
Folic Acid ◽  
Cancer Cells ◽  
Carboxyl Groups ◽  
Target Cells ◽  
Intracellular Uptake ◽  
Cellular Targeting ◽  
Specific Targeting ◽  
The Ideal

Targeting of drugs and therapeutic materials to target cells or designated intracellular locations relies upon their cellular / sub-cellular targeting and trafficking. The ideal optical properties of quantum dots offer the possibility of using them as fluorescent probes to study the intracellular uptake and pathway of drugs or biomolecules. Quantum dots, ZnS coated CdSe, were synthesized and successfully incorporated into polystyrene (PS) particles grafted with carboxyl groups and folic acid was attached to the nanoparticle surfaces. The nanocomposites were monodisperse and highly luminescent, and their intracellular uptake to cancer cells was investigated using confocal microscopy.

Optical properties of core-multishell quantum dots and their imaging applications in cancer cells

2007 ◽  
Author(s):  
Y. Liu ◽  
P. Chen ◽  
L. Lin ◽  
G.-Q. Tang ◽  
G.-G. Mu
Keyword(s):  
Quantum Dots ◽  
Optical Properties ◽  
Cancer Cells

Folic acid-conjugated core/shell ZnS:Mn/ZnS quantum dots as targeted probes for two photon fluorescence imaging of cancer cells

2011 ◽  
Vol 7 (3) ◽  
pp. 1327-1338 ◽  
Author(s):  
Malgorzata Geszke ◽  
Marek Murias ◽  
Lavinia Balan ◽  
Ghouti Medjahdi ◽  
Jaroslaw Korczynski ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Folic Acid ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Core Shell ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals PEGylated-folic acid–modified black phosphorus quantum dots as near-infrared agents for dual-modality imaging-guided selective cancer cell destruction

Nanophotonics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 2425-2435 ◽  
Author(s):  
Jing Wang ◽  
Dong Liang ◽  
Zehua Qu ◽  
Ivan M. Kislyakov ◽  
Valery M. Kiselev ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Folic Acid ◽  
Cancer Cells ◽  
Near Infrared ◽  
Processing System ◽  
Black Phosphorus ◽  
Optical Absorbance ◽  
Present Design ◽  
Black Phosphorus Quantum Dots

AbstractBiological systems have high transparence to 700–1100-nm near-infrared (NIR) light. Black phosphorus quantum dots (BPQDs) have high optical absorbance in this spectrum. This optical property of BPQDs integrates both diagnostic and therapeutic functions together in an all-in-one processing system in cancer theranostic approaches. In the present study, BPQDs were synthesized and functionalized by targeting moieties (PEG-NH2-FA) and were further loaded with anticancer drugs (doxorubicin) for photodynamic–photothermal–chemotherapy. The precise killing of cancer cells was achieved by linking BPQDs with folate moiety (folic acid), internalizing BPQDs inside cancer cells with folate receptors and NIR triggering, without affecting the receptor-free cells. These in vitro experiments confirm that the agent exhibited an efficient photokilling effect and a light-triggered and heat-induced drug delivery at the precise tumor sites. Furthermore, the nanoplatform has good biocompatibility and effectively obliterates tumors in nude mice, showing no noticeable damages to noncancer tissues. Importantly, this nanoplatform can inhibit tumor growth through visualized synergistic treatment and photoacoustic and photothermal imaging. The present design of versatile nanoplatforms can allow for the adjustment of nanoplatforms for good treatment efficacy and multiplexed imaging, providing an innovation for targeted tumor treatment.

Folic Acid Immobilized Ferrimagnetic DP-Bioglass to Target Tumor Cell for Cancer Hyperthermia Treatment

2006 ◽  
Vol 53 ◽  
pp. 50-57 ◽  
Author(s):  
Min Hua Chen ◽  
Chung King Hsu ◽  
Feng Huei Lin ◽  
Leszek Stobinski ◽  
Jerzy Peszke
Keyword(s):  
Folic Acid ◽  
Biological Study ◽  
Target Cells ◽  
Intracellular Uptake ◽  
Hyperthermia Treatment ◽  
Amino Silane ◽  
Target Tumor Cell ◽  
Surface Modified ◽  
Drug And Gene Delivery

DP-bioglass is one of biodegradable glasses, which can be used as bioactive material in soft tissue and bone. It was often used in orthopedy and plastic surgery. Recently, bioglass was also used as a carrier for drug and gene delivery systems. Additionally, ferrimagnetic DP-bioglass can be potential candidates for magnetic induction hyperthermia, by using a magnetic field. The aim of this work is the preparation and characterization of surface-modified ferrimagnetic DP-bioglass. First DP-bioglass has to be surface-modified with polyethylene glycol (PEG) and folic acid (FA) to improve its intracellular uptake and ability to target specific cells. PEG-FA complex was synthesized using carbodiimide (DCC) to link PEG with FA. Then PEG-FA complex were immobilized on the surface of DP-bioglass by using amino-silane (AEAPS) as a coupling agent. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H NMR), and thermogravimetric analysis (TGA) was used to demonstrate this immobilization process. In biological study showed that immobilized ferromagnetic DP-bioglass with PEG-FA was non-cytotoxicity and significantly enhanced the intracellular uptake of DP-bioglass by target cells.

scholarly journals Targeted delivery and enhanced gene-silencing activity of centrally modified folic acid–siRNA conjugates

2019 ◽  
Vol 48 (1) ◽  
pp. 75-85 ◽  
Author(s):  
Lidya Salim ◽  
Golam Islam ◽  
Jean-Paul Desaulniers
Keyword(s):  
Folic Acid ◽  
Gene Silencing ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Target Cells ◽  
Gene Target ◽  
Folate Receptors ◽  
Normal Tissues ◽  
Sense Strand ◽  
Low Cytotoxicity

Abstract One of the major hurdles in RNAi research has been the development of safe and effective delivery systems for siRNAs. Although various chemical modifications have been proposed to improve their pharmacokinetic behaviour, their delivery to target cells and tissues presents many challenges. In this work, we implemented a receptor-targeting strategy to selectively deliver siRNAs to cancer cells using folic acid as a ligand. Folic acid is capable of binding to cell-surface folate receptors with high affinity. These receptors have become important molecular targets for cancer research as they are overexpressed in numerous cancers despite being expressed at low levels in normal tissues. Employing a post-column copper-catalyzed alkyne–azide cycloaddition (CuAAC), we report the synthesis of siRNAs bearing folic acid modifications at different positions within the sense strand. In the absence of a transfection carrier, these siRNAs were selectively taken up by cancer cells expressing folate receptors. We show that centrally modified folic acid–siRNAs display enhanced gene-silencing activity against an exogenous gene target (∼80% knockdown after 0.75 μM treatment) and low cytotoxicity. In addition, these siRNAs achieved potent dose-dependent knockdown of endogenous Bcl-2, an important anti-apoptotic gene.

Aligning CdS Quantum Dots in Apo-Ferritin Protein and PS-b-P2VP Organic Templates

2013 ◽  
Vol 832 ◽  
pp. 675-680
Author(s):  
S.A. Shamsudin ◽  
Hirokazu Hasegawa ◽  
Mikihito Takenaka ◽  
Saijo Kenji
Keyword(s):  
Quantum Dots ◽  
Optical Properties ◽  
Cancer Cells ◽  
Cadmium Sulphide ◽  
Cds Quantum Dots ◽  
Organic Templates ◽  
Ps I ◽  
Cds Qds ◽  
Guest System ◽  
Photo Stability

Quantum dots (QDs) have a great potential to be applied as a biosensor to detect cancer cells because of their properties such as high photoluminescence, photo stability, narrow peak of luminescence, etc. However, cadmium sulphide (CdS) quantum dots are toxic and hydrophobic, making it impossible to be applied into biomaterials. A template is needed to make CdS QDs nontoxic and hydrophilic. The optical properties of these QDs are needed to be protected. To achieve this purpose, the template for CdS QDs has been designed by capping CdS QDs with apo-ferritin shells before aligning them onto cylindrical perpendicular PS-b-P2VP template, with the aim of locating CdS QDs appropriately in sequence. A host-guest system can be developed.

SYNTHESIS OF POLYSTYRENE ENCAPSULATEDZnS-COATEDCdSeNANOCOMPOSITES MODIFIED WITH PLL–PEI–PEG–FA

2005 ◽  
Vol 04 (02) ◽  
pp. 229-235 ◽  
Author(s):  
JIANLING CAI ◽  
NING HUANG ◽  
YONG ZHANG
Keyword(s):  
Quantum Dots ◽  
Folic Acid ◽  
Biomedical Applications ◽  
Zinc Sulphide ◽  
Target Cells ◽  
Side Chains ◽  
Folate Receptors ◽  
Current Cell ◽  
Poly Ethylene ◽  
Polymerization Method

Current cell tags using dyes lose their luminescence quickly and are not suitable for optical barcoding. Semiconductor quantum dots (QDs), on the other hand, can be engineered to emit different wavelengths, thus permitting tagging of various cells at the same time. Core/shell luminescent quantum dots, cadmium selenide ( CdSe ) and zinc sulphide ( ZnS ), were synthesized and incorporated into polystyrene (PS) particles grafted with carboxyl groups using microemulsion polymerization method. Highly luminescent monodispersed PS particles with diameters from 30 nm to 50 nm were chosen for subsequent surface modification. Two series of surface modifications were carried out with PS particles. One was modified with poly(L-Lysine) (PLL), polyethylenimine (PEI), Poly(Ethylene Glycol) (PEG) and folic acid (FA) side chains in sequence through chemical bonding. Another was conjugated with PEG and FA in sequence. The folic acid side chains can enter target cells via folate receptors and assisted in the uptake of the luminescent particles into the cells. This property allows them to be used as fluorescent labels for marking their ingress into cells and also for a cornucopia of biomedical applications.

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