scholarly journals Dissolution capacity of orange and eucalyptus oils versus endodontic types of cement (in vitro study)

2022 ◽  
Vol 10 (19) ◽  
pp. 29-33
Author(s):  
Sebastián M. Ulloa-Alvarez ◽  
María E. Guerrero-Coello ◽  
José E. Torracchi-Carrasco
Keyword(s):  
In Vitro Study ◽  
Control Group ◽  
Filling Material ◽  
Cement Sample ◽  
Orange Oil ◽  
Eucalyptus Oil ◽  
Before And After ◽  
The Difference ◽  
Post Hoc

Introduction: Endodontic retreatment seeks reentry to the root canal system to remove all filling material, identification and correction of pathological or iatrogenic defects. Objective: This study aimed to compare the dissolving capacity of eucalyptus oil, orange oil, and distilled water versus three types of endodontic cements. Materials and methods: Two hundred and four stainless steel molds were fabricated, where each cement sample was placed to the edge and left to set in the incubator, simulating normal oral conditions for 7 days. Subsequently, the solubility of each cement was analysed by immersing the samples in eucalyptus and orange oil for ten minutes in a static and ultrasonic environment. The solubility of the cement was evidenced by the difference between the weight before and after contact with the oils, data were statistically analysed with the ANOVA test and the post hoc Tukey bootstrap means test with 1,000 repetitions. Results: The control group and eucalyptus oil having no significant effect (p>0.05), the ultrasonic environment was found to be effective (p<0.05), whereas orange oil had better dissolution effects (p=0.004). Conclusions: The most susceptible cement was Sealapex, while the least soluble was MTA-Fillapex in all the solvents studied.

scholarly journals Comparative Evaluation of Influence of Three Different Obturation Techniques on Removal of Filling Material during Retreatment: An in vitro Study

2020 ◽  
pp. 35-40
Author(s):  
Deebah Choudhary
Keyword(s):  
Comparative Evaluation ◽  
In Vitro Study ◽  
Filling Material ◽  
Micro Computed Tomography ◽  
Jammu And Kashmir ◽  
Before And After ◽  
Conservative Dentistry ◽  
Time Required ◽  
Post Hoc

Aim: To evaluate the influence of various obturating techniques on retreatment teeth. Place and Duration of Study: Department of Conservative Dentistry and Endodontics, Institute of Dental Sciences, Jammu and Kashmir, India between December 2019 and February 2020. Methodology: Sixty extracted mandibular premolars were randomly divided into three groups (n=20) based on the obturation technique adopted which endodontic treatment i.e., cold lateral compaction, thermoplasticized and GuttaFlow techniques. The samples were evaluated using micro-computed tomography for the volume before and after the retreatment to assess the remaining amount of filling material in the canals, and also the time taken for the removal of filling material during the retreatment. Data were analyzed using ANOVA followed by Post hoc test at P < 0.05. Results: The percentage of remaining filling material was between 17%-27%. The highest percentage of remaining filling material was seen in samples obturated with GuttaFlow (P < 0.05). The time required for retreatment was highly significant in thermoplasticized technique (P < 0.05). Conclusion: The type of obturating technique influenced the amount of filling material remained in the canal after retreatment and also the time taken during this removal.

scholarly journals Potential of different fluoride gels to prevent erosive tooth wear caused by gastroesophageal reflux

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Philipp Körner ◽  
Luca Georgis ◽  
Daniel B. Wiedemeier ◽  
Thomas Attin ◽  
Florian J. Wegehaupt
Keyword(s):  
Gastroesophageal Reflux ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Tooth Wear ◽  
Control Group ◽  
Erosive Tooth Wear ◽  
Custom Made ◽  
Post Hoc ◽  
Bovine Enamel

Abstract Background This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear. Methods Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1–5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0.5 ml/min). Reflux was simulated 11 times a day during 12 h by adding HCl (pH 3.0) for 30 s (flow rate 2 ml/min). During the remaining 12 h (overnight), specimens were stored in artificial saliva and brushed twice a day (morning and evening) with a toothbrush and toothpaste slurry (15 brushing strokes). While specimens in the control group (G1) did not receive any further treatment, specimens in G2–5 were coated with different fluoride gels [Elmex Gelée (G2); Paro Amin Fluor Gelée (G3); Paro Fluor Gelée Natriumfluorid (G4); Sensodyne ProSchmelz Fluorid Gelée (G5)] in the evening for 30 s. After 20 days, surface profiles were recorded again and enamel loss was determined by comparing them with the baseline profiles. The results were statistically analysed using one-way analysis of variance (ANOVA) followed by Tukey`s HSD post-hoc test. Results The overall highest mean wear of enamel (9.88 ± 1.73 µm) was observed in the control group (G1), where no fluoride gel was applied. It was significantly higher (p < 0.001) compared to all other groups. G2 (5.03 ± 1.43 µm), G3 (5.47 ± 0.63 µm, p = 0.918) and G4 (5.14 ± 0.82 µm, p > 0.999) showed the overall best protection from hydrochloric acid induced erosion. Enamel wear in G5 (6.64 ± 0.86 µm) was significantly higher compared to G2 (p = 0.028) and G4 (p = 0.047). Conclusions After 20 days of daily application, all investigated fluoride gels are able to significantly reduce gastroesophageal reflux induced loss of enamel.

scholarly journals Comparison of the Remineralizing Effect of Brushing with Aloe vera versus Fluoride Toothpaste

2020 ◽  
Author(s):  
Teresa Al Haddad ◽  
Elie Khoury ◽  
Nada Farhat Mchayleh
Keyword(s):  
Repeated Measures ◽  
Aloe Vera ◽  
In Vitro Study ◽  
P Value ◽  
Fluoride Toothpaste ◽  
Before And After ◽  
The Difference ◽  
Group B ◽  
Ca:P Ratio

Abstract Objectives The aim of the present in vitro study is to compare the remineralization brushing effect of three toothpastes and Aloe vera (AV) gel. Materials and Methods Forty sound extracted teeth were placed in a demineralizing solution for 4 days and randomly assigned to four groups: group A: 1,450-ppm fluoride toothpaste; group B: AV nonfluoridated toothpaste; group C: AV 1,000-ppm fluoridated toothpaste; and group D: AV gel. A 3-minute pH cycling was performed twice a day for each group for 12 days. Specimens were analyzed before and after by scanning electron microscope—energy dispersive X-ray. Statistical analysis The outcomes were analyzed by Kolmogorov–Smirnov’s tests, repeated-measures analyses of variance followed by univariate analyses, and Bonferroni’s multiple comparisons tests to compare the calcium-to-phosphorus (Ca:P) ratio within time among toothpaste groups. Results Following remineralization, the Ca:P ratio increased in all groups. The difference of the Ca:P ratio was not significant between groups C, D, and A. The mean ratio was significantly lower in group B (p-value = 0.026). Conclusions The AV gel demonstrated a remineralization capacity equal to that of the 1,450-ppm fluoride toothpaste. In contrast, fluoride-free AV toothpaste showed a lower remineralization efficiency. Further studies are required to understand its mechanism.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

scholarly journals The role of beluntas (pluchea indica less.) leaf extract in preventing the occurrence of fibroblasts hyperproliferation: An in vitro preliminary study

2019 ◽  
Author(s):  
Sakti Charlia Maharani ◽  
Indah Julianto ◽  
Suci Widhiati
Keyword(s):  
Leaf Extract ◽  
In Vitro Study ◽  
Collagen I ◽  
Control Group ◽  
Antibody Treatment ◽  
Average Decrease ◽  
Matrix Deposition ◽  
Post Hoc

Beluntas (Pluchea indica Less.) is a herbal plant which contains variety of benefits. Quercetin, one of flavonoid, is the most bioactive agent in beluntas leaf. Collagen inhibition by quercetin may modulate extracellular matrix deposition and inhibit the formation of hypertrophic scar. This was an in vitro study with senescent fibroblasts to determine the role of beluntas leaf extract in preventing the occurence of fibroblasts hyperproliferations. There were 4 groups were stained by anti-collagen I antibodies and secondary antibody. Flowcytometry analysis was done to measure the fibroblasts density. Anova test was performed with a value of p=0.000 (p<0.05). A post hoc analysis showed significant differences in the average decrease of fibroblasts that absorbs staining anti-collagen I antibody treatment group compared with the control group. There were significant effects of beluntas leaf extract in preventing the occurrence of fibroblasts hyperproliferations. Beluntas leaf extract with a concentration of 80 mol/L had the most significant effect on the fibroblasts density. Thus beluntas leaf extract has the ability in preventing the occurrence of fibroblasts hyperproliferation.

scholarly journals The difference of the salivary volume before and after drinking the rosella tea (Hibiscus sabdariffa)

2010 ◽  
Vol 22 (3) ◽  
Author(s):  
Mutiara Indah Permata Sari Islami ◽  
Edeh Roletta Haroen ◽  
Sri Tjahajawati
Keyword(s):  
In Vitro Study ◽  
T Test ◽  
Hibiscus Sabdariffa ◽  
Test Analysis ◽  
Community Benefits ◽  
Significant Difference ◽  
Before And After ◽  
Quasi Experimental ◽  
The Difference

Introduction: Roselle plants (Hibiscus sabdariffa) is one of the herbs that began to be used by the community. Benefits of this plant is quite a lot for health. The portion taken for consumption is the red flower petals. oselle tea is one of the sour beverages which can affect the salivary gland secretion. The purpose of this study is to analyzed the difference of salivary volume before and after drinking roselle tea. Methods: This study has been conducted to 40 students of Faculty of Dentistry, Padjadjaran University, ranging from 18-23 years of age with good general condition. This study is quasi-experimental in vitro study using the paired test analysis with α = 0,05 of the data collected from salivary volume. Results: The result of study indicates that the average of salivary volume before drinking roselle tea is 1,90 milliliter. After drinking roselle tea, the average of salivary volume is 4,54 milliliter. The result of paired test analysis shows that t-test is 16,172 and t-table is 2,022. The value of t-test > t-table. Result of statistic shown there is significant difference of salivary volume before and after drinking roselle tea. Conclusion: There is a difference of salivary volume before and after drinking roselle tea.

scholarly journals An Evaluation of GuttaFlow2 in Filling Artificial Internal Resorption Cavities: An in vitro Study

2016 ◽  
Vol 17 (6) ◽  
pp. 445-450 ◽  
Author(s):  
Mohammad Y Hajeer ◽  
Yara Mohammad ◽  
Hisham Alafif ◽  
Oula Yassin
Keyword(s):  
Cross Sections ◽  
Root Canal ◽  
In Vitro Study ◽  
Free Flow ◽  
Control Group ◽  
Injection Technique ◽  
Filling Material ◽  
The Third ◽  
Gutta Percha

ABSTRACT Introduction Obturation of root canal with internal resorption represents a major challenge in Endodontics. In spite of that, usual obturation techniques are often employed without considering the best technique to solve this problem. The goal of this study was to investigate the ability of GuttaFlow2 in filling artificial internal resorption cavities. Materials and methods The study sample included 36 human upper central incisors that were prepared using Protaper system (F4). Internal resorption cavities were prepared by cutting each tooth at 7 mm from the apex and preparing hemispherical cavities on both the sides and then re-attaching them. The sample was randomly separated into three groups (n = 12 in each group). In the first group, thermal injection technique (Obtura II) was employed and served as the control group. In the second group, injection of cold free-flow obturation technique with a master cone (GF2-C) was employed, whereas in the third group injection of cold free-flow obturation without a master cone (GF2) was followed. The teeth were re-cut at the same level as before and examined under a stereomicroscope. Subsequently, the captured images were transferred to AutoCAD program to measure the percentage of total filling “TF,” gutta-percha “G,” sealer “S,” and voids “V” out of the total surface of the cross sections. Results All materials showed high filling properties in terms of “total filling,” ranging from 99.17% (for Obtura II) to 99.72% (for GF2-C). Regarding gutta-percha percentages of filling, they ranged from 83.15 to 83.93%, whereas those for the sealer ranged from 5.71 to 15.24%. GuttaFlow2 group with a master cone appeared to give the best results despite the insignificant differences among the three groups. Conclusion The GuttaFlow2 with a master cone technique seemed to be a promising filling material and gave results similar to those observed with Obtura II. It is recommended for use to obturate internal resorption cavities in clinical practice due to its good adaptability to root canal walls, ease of handling, and application. Clinical significance Internal resorption defects can be successfully filled with GuttaFlow2 material when supplemented with a master cone, and the results are comparable with those obtained with the Obtura II technique. How to cite this article Mohammad Y, Alafif H, Hajeer MY, Yassin O. An Evaluation of GuttaFlow2 in Filling Artificial Internal Resorption Cavities: An in vitro Study. J Contemp Dent Pract 2016;17(6):445-450.

scholarly journals Analysis of Surface Roughness and 3D Scanning Topography of Zirconia Implants Before and After Photofunctionalisation by 3D Optical Profilometry- An Invitro Study

2021 ◽  
Author(s):  
Arunjaikumar Ravindran ◽  
Suma Karthigeyan ◽  
Ramesh Bhat ◽  
Madhulika Naidu ◽  
Senthilnathan Natarajan ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Roughness Parameter ◽  
In Vitro Study ◽  
Control Group ◽  
P Value ◽  
Study Group ◽  
Optical Profilometry ◽  
Before And After ◽  
Zirconia Implants

Introduction: Zirconia is considered as an alternative material for the fabrication of implants. Surface roughness of the implant plays a fundamental role in the initial bone formation. The rationale of using 3D optical profilometry in this study was to evaluate the surface roughness of the zirconia implants before and after Ultraviolet (UV) photofunctionalisation. Also, Moreso 3D optical instruments have a better resolution than the mechanical ones like Atomic Force Microscopy (AFM). Aim: To analyse the surface roughness and topography of zirconia implants after photofunctionalisation using optical profilometry. Materials and Methods: This is an in-vitro study conducted over a period of six months from March 2020 to August 2020. Ten commercially machined Zirconia implants, five each in study and control group, were micro analysed at three different regions (abutment, thread and crest) by optical profilometry. Study group was surface treated by UV radiation for 48 hours. Quantitative morphometric analysis was done between two groups and p-value less than 0.05 was considered statistically significant. The statistical test applied in this study was independent t-test. Results: Scanning micrographs of the study group revealed highest density of summits contributing to increased surface area in the study group. Quantitative analysis of surface roughness showed statistically significant higher mean roughness parameter for photofunctionalised implants in abutment, crest and thread region (p<0.05). Conclusion: Photofunctionalisation is a potentially synergistic technique in producing textured zirconia implants.

scholarly journals In vitro evaluation of gutta-percha dissolution ability of different solvents

RSBO ◽  
2017 ◽  
Vol 14 (3) ◽  
pp. 152-05
Author(s):  
Kelly Cristina Santana de Oliveira ◽  
Flávia Sens Fagundes Tomazinho ◽  
Monique Marchiori ◽  
Marilisa Carneiro Leão Gabardo ◽  
Sérgio Herrero Moraes ◽  
...  
Keyword(s):  
Statistical Test ◽  
Control Group ◽  
Filling Material ◽  
Time Intervals ◽  
Orange Oil ◽  
Loss Analysis ◽  
Gutta Percha ◽  
Significant Difference ◽  
Endodontic Retreatment

The removal of the filling material during the Endodontic retreatment is essential, so a better action of solvents on the gutta-percha is desired. Objective: To evaluate in vitro the gutta-percha dissolution ability of different solvents. Material and methods: Four solvents (xylol, eucalyptol, citrol, and orange oil) were applied on gutta-percha points, which had been later submitted to the weight loss analysis at 2, 5, and 10 minutes. For each solvent, 30 size #60 main cones were used, divided into three groups. Distilled water was used as control group. The statistical test applied was Kruskal-Wallis. Results: No statistically significant difference occurred among the solvents at 2 minutes (p > 0.05); however, during this period, citrol had the best performance. In longer time intervals, the xylol showed differences compared to eucalyptol at 5 minutes and to eucalyptol and citrol at 10 minutes (p < 0.05). The worst results were found for eucalyptol. Conclusion: This xylol was the solvent with improved ability to dissolve the gutta-percha points, with better results at longer time periods.

scholarly journals Effect of Incorporation of Disinfectants on Surface Hardness and Dimensional Accuracy of Die Stone: An in vitro Study

2017 ◽  
Vol 7 (3) ◽  
pp. 86-91
Author(s):  
Anubhuti Bakshi ◽  
Rajat Dang
Keyword(s):  
Sodium Hypochlorite ◽  
Testing Machine ◽  
Surface Hardness ◽  
In Vitro Study ◽  
Dimensional Accuracy ◽  
Control Group ◽  
Time Interval ◽  
Type Iv ◽  
Post Hoc

ABSTRACT Aim To evaluate surface hardness and dimensional accuracy of type IV dental stone after incorporation of disinfectants. Materials and methods A total of 100 specimens, 50 (40 mm⨯ 10 mm) disk shaped and 50 frustum cone shaped, were fab- ricated from type IV dental stone after incorporation of various disinfectants, namely glutaraldehyde (2%), iodophor (1.76%), chlorhexidine (2%), and sodium hypochlorite (1%), for evaluation of surface hardness and dimensional accuracy. Surface hardness was tested after 1- and 24-hour time interval using Rockwell hard- ness testing machine. Dimensional accuracy was measured using traveling microscope. Data obtained were compiled and analyzed statistically by using analysis of variance and post hoc test. Results Results showed significant decrease in surface hard- ness of samples incorporated with disinfectants at 1 hour as compared with control (distilled water). Surface hardness of all the samples increased with time and was almost equal to control group at 24-hour interval, with iodophor showing the maximum value in comparison with the other disinfectants used. When dimensional accuracy of all the groups was compared, iodophor and sodium hypochlorite were found to cause negative alterations. Conclusion Within the limitations of this in vitro study, it was concluded that incorporation of disinfectants in die stone during mixing did not affect the hardness value at 24-hour interval. Dimensional accuracy was altered by iodophor and sodium hypochlorite, but remained unaffected by chlorhexidine and glutaraldehyde. How to cite this article Bakshi A, Dang R. Effect of Incorpo- ration of Disinfectants on Surface Hardness and Dimensional Accuracy of Die Stone An in vitro Study. Int J Prosthodont Restor Dent 2017;7(3):86-91.

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